##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552809_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1450471
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.612392802062228	31.0	31.0	34.0	30.0	34.0
2	31.786467981779712	31.0	31.0	34.0	30.0	34.0
3	31.823367030433563	31.0	31.0	34.0	30.0	34.0
4	35.45105969026613	37.0	35.0	37.0	33.0	37.0
5	35.33375917202068	37.0	35.0	37.0	33.0	37.0
6	35.30548283971206	37.0	35.0	37.0	32.0	37.0
7	35.75445768995037	37.0	35.0	37.0	35.0	37.0
8	35.74336405209067	37.0	35.0	37.0	35.0	37.0
9	37.33541587525707	39.0	37.0	39.0	35.0	39.0
10	36.72461221217108	39.0	37.0	39.0	32.0	39.0
11	36.542475513126426	38.0	35.0	39.0	32.0	39.0
12	36.44206054447142	38.0	35.0	39.0	32.0	39.0
13	36.396987599200536	38.0	35.0	39.0	32.0	39.0
14	37.43874024368636	39.0	36.0	41.0	32.0	41.0
15	37.540833977377005	39.0	36.0	41.0	32.0	41.0
16	37.59520528159474	39.0	36.0	41.0	33.0	41.0
17	37.58522231744033	39.0	36.0	41.0	32.0	41.0
18	37.55094793346437	39.0	36.0	41.0	32.0	41.0
19	37.539835680961566	39.0	36.0	41.0	32.0	41.0
20	37.48206065478041	39.0	36.0	41.0	32.0	41.0
21	37.43714489982909	39.0	36.0	41.0	32.0	41.0
22	37.386316582682454	39.0	36.0	41.0	32.0	41.0
23	37.34894872079483	39.0	36.0	41.0	32.0	41.0
24	37.32376517696665	39.0	36.0	41.0	32.0	41.0
25	37.27006262103827	39.0	35.0	41.0	32.0	41.0
26	37.1814624353055	39.0	35.0	40.0	32.0	41.0
27	37.14128583060261	39.0	35.0	40.0	32.0	41.0
28	37.07501976944041	39.0	35.0	40.0	31.0	41.0
29	37.07363952812569	39.0	35.0	40.0	31.0	41.0
30	36.98555710524374	39.0	35.0	40.0	31.0	41.0
31	36.85493332855328	39.0	35.0	40.0	31.0	41.0
32	36.7916462997192	39.0	35.0	40.0	31.0	41.0
33	36.70108881873543	39.0	35.0	40.0	30.0	41.0
34	36.603345396081686	39.0	35.0	40.0	30.0	41.0
35	36.58494792381234	39.0	35.0	40.0	30.0	41.0
36	36.50876577332466	39.0	35.0	40.0	30.0	41.0
37	36.44768906100156	39.0	35.0	40.0	30.0	41.0
38	36.43082764150404	39.0	35.0	40.0	30.0	41.0
39	36.378304012972336	39.0	35.0	40.0	30.0	41.0
40	36.274719728970794	38.0	35.0	40.0	30.0	41.0
41	36.16928570098954	38.0	35.0	40.0	30.0	41.0
42	36.10915075172134	38.0	35.0	40.0	30.0	41.0
43	36.0415858021291	38.0	35.0	40.0	29.0	41.0
44	35.907383877375004	38.0	35.0	40.0	29.0	41.0
45	35.84544606545047	38.0	34.0	40.0	29.0	41.0
46	35.70689796624683	38.0	34.0	40.0	28.0	41.0
47	35.59235103631855	38.0	34.0	40.0	28.0	41.0
48	35.54560070487449	38.0	34.0	40.0	28.0	41.0
49	35.499580481098896	38.0	34.0	40.0	28.0	41.0
50	35.37707062050879	38.0	34.0	40.0	28.0	41.0
51	33.79052321625183	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	5.0
12	14.0
13	16.0
14	23.0
15	53.0
16	87.0
17	236.0
18	488.0
19	818.0
20	1356.0
21	2107.0
22	3166.0
23	4673.0
24	6612.0
25	8958.0
26	11772.0
27	15013.0
28	19187.0
29	24960.0
30	31570.0
31	40405.0
32	52319.0
33	69177.0
34	103773.0
35	121174.0
36	135617.0
37	187067.0
38	289666.0
39	320113.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.558707481914496	20.84267799907754	26.648723069954517	10.949891449053444
2	31.279356843397764	23.966352998439817	26.212106274444647	18.542183883717772
3	31.51748638890402	24.521896680457587	27.1284982602203	16.83211867041809
4	28.601261245485087	26.20493618969287	26.313728437176614	18.880074127645432
5	24.88060774741446	30.550628037375443	24.88819149090192	19.680572724308174
6	26.26402044577244	35.653866916332696	23.626325517711145	14.45578712018372
7	81.23085535663932	5.652715566185053	8.036217201171207	5.080211876004415
8	81.22775291612173	4.822295654308152	8.017947273678688	5.932004155891431
9	75.765734027085	7.259710811177886	10.56477516613569	6.409779995601429
10	39.443601423261825	28.818501024839517	17.42151342563898	14.316384126259676
11	27.806002326140955	25.657665682388686	26.144955673019314	20.39137631845104
12	25.498062353538952	23.615846163073925	30.897894545978517	19.988196937408606
13	23.369719215344535	25.084679390349756	31.709355099136765	19.836246295168948
14	19.26712081799636	28.617462879299204	29.523789169173327	22.59162713353111
15	18.916476096385242	26.69636276768029	33.884096958849916	20.503064177084546
16	20.213572005231402	26.7358671769377	30.921473093912255	22.129087723918644
17	20.450253745162776	25.97094323154341	29.103305064354956	24.475497958938856
18	20.94595479675223	25.820302508633404	30.722227469559886	22.51151522505448
19	22.288484223400538	27.36904081501802	28.0954944979941	22.246980463587345
20	23.762350298627137	26.91491246636437	28.8802051195784	20.442532115430094
21	22.494899932504683	28.16519599495612	28.77941027431779	20.560493798221405
22	20.64226034164075	26.311660143498216	27.279414755620763	25.766664759240278
23	20.590828772171246	27.500515349841535	27.68797170022703	24.22068417776019
24	21.78499259895579	25.93984988324482	28.891994393545268	23.38316312425412
25	20.924168770006432	27.896524646132185	26.305524205585634	24.873782378275745
26	21.073085914851106	27.87722057180047	27.504445107830488	23.54524840551793
27	22.276005518207533	26.685538697430005	27.87653114057434	23.16192464378812
28	18.927024394145075	26.59446483245787	29.549436010785463	24.929074762611595
29	21.550930697683718	24.819868856392166	27.436329302688574	26.192871143235543
30	21.987823265684042	25.566729703661778	26.40983515009952	26.03561188055466
31	21.642073505778466	27.787663455525824	25.908756534946235	24.66150650374947
32	22.309511875797586	26.937870526194597	25.28413184406996	25.46848575393786
33	20.683626215208715	27.29665053627408	24.790843801771974	27.22887944674523
34	19.646101163001536	26.4715392448384	28.06702098835482	25.815338603805248
35	21.03923484164799	24.5718115012296	26.577918483030682	27.811035174091725
36	20.906312501249595	28.020898039326536	26.7727517475358	24.30003771188807
37	19.54172127536504	27.97877379140982	29.312064839627954	23.167440093597182
38	19.033610461705198	27.03308097852353	27.6374363913515	26.295872168419777
39	21.30825090608499	26.54958285963663	26.515318127697828	25.626848106580553
40	21.621528455239712	25.79961957184942	28.99954566482198	23.579306308088892
41	19.205692495747932	27.299752976791673	27.541467564673823	25.95308696278657
42	21.091011126730557	25.646083237789657	28.4102198527237	24.852685782756083
43	21.689506374136403	23.92643493044673	28.84966331626072	25.534395379156148
44	20.50940694436497	24.22971572682253	27.468801513439427	27.792075815373074
45	21.365060039118326	23.017419858790696	26.953451671905192	28.66406843018578
46	21.60422373146378	24.621105833898092	28.110799871214248	25.66387056342388
47	18.76466334039081	25.414779061422117	30.83818980179542	24.982367796391657
48	18.706475344905208	23.900994918202432	30.590201389755467	26.802328347136893
49	20.816479612484496	23.365375798619898	30.513950296145183	25.304194292750427
50	19.327446050282976	22.65222813830818	30.012526965378832	28.007798846030013
51	18.971354821985408	22.409479403586836	27.300993952998713	31.31817182142904
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	555.0
1	870.0
2	1185.0
3	1705.0
4	2225.0
5	1779.5
6	1334.0
7	1350.0
8	1366.0
9	1507.5
10	1649.0
11	1723.5
12	1798.0
13	1944.0
14	2090.0
15	2336.0
16	2582.0
17	2710.5
18	2839.0
19	3067.5
20	3296.0
21	3682.0
22	4068.0
23	5193.0
24	6318.0
25	7952.0
26	11369.5
27	13153.0
28	16363.0
29	19573.0
30	22430.0
31	25287.0
32	28644.5
33	32002.0
34	35105.0
35	38208.0
36	40760.0
37	43312.0
38	46110.5
39	48909.0
40	55395.0
41	61881.0
42	69936.5
43	77992.0
44	87139.0
45	96286.0
46	114083.0
47	131880.0
48	154451.5
49	177023.0
50	170307.5
51	163592.0
52	137479.5
53	111367.0
54	96809.5
55	82252.0
56	72236.5
57	62221.0
58	58332.5
59	54444.0
60	51398.5
61	48353.0
62	43832.0
63	39311.0
64	33569.0
65	27827.0
66	23030.0
67	18233.0
68	15189.0
69	12145.0
70	10715.5
71	9286.0
72	7819.0
73	6352.0
74	5151.5
75	3049.0
76	2147.0
77	1640.0
78	1133.0
79	991.0
80	849.0
81	585.0
82	321.0
83	220.5
84	120.0
85	85.5
86	51.0
87	58.0
88	65.0
89	42.5
90	20.0
91	23.5
92	27.0
93	16.0
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1450471.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.677881996522398
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.81496530846614	15.994960654910203
2	13.777140582297731	6.7998129907888645
3	6.110316106845575	4.523689795385557
4	3.317027261908998	3.2742882939455216
5	1.9426159921519526	2.3969824109441586
6	1.277475714103336	1.8915236955639185
7	0.9122498060682475	1.5758675145851855
8	0.6467135088462868	1.2767615725492485
9	0.5022441768353192	1.115489027644428
>10	5.425795259417384	33.898556099089504
>50	1.1589904600466656	18.40769343868959
>100	0.10131204878133573	4.503477211614981
>500	0.008955761178591577	1.473509892662938
>1k	0.003358410441971841	1.4249569301814735
>5k	8.396026104929603E-4	1.4424304714443619
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	7425	0.5119026854035689	Illumina PCR Primer Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	7065	0.4870831612627898	RNA PCR Primer, Index 14 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	6395	0.44089126911189536	Illumina PCR Primer Index 7 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	4000	0.2757724904531011	RNA PCR Primer, Index 14 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	2439	0.1681522760537784	RNA PCR Primer, Index 14 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	2155	0.14857242923160824	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	1747	0.12044363520539192	RNA PCR Primer, Index 14 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1678	0.11568655974507593	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5316893615935789	0.0
2	0.0	0.0	0.0	1.9814942870281447	0.0
3	0.0	0.0	0.0	2.7350426171912434	0.0
4	0.0	0.0	0.0	3.5668413915204096	0.0
5	0.0	0.0	0.0	5.532409817224887	0.0
6	0.0	0.0	0.0	6.517744925613818	0.0
7	0.0	0.0	0.0	7.6158020394754535	0.0
8	0.0	0.0	0.0	9.477955781260018	0.0
9	0.0	0.0	0.0	10.075761597439728	0.0
10	0.0	0.0	0.0	11.662142848771193	0.0
11	0.0	0.0	0.0	14.125549562866132	0.0
12	0.0	0.0	0.0	15.931445716598263	0.0
13	0.0	0.0	0.0	16.60198652713498	0.0
14	0.0	0.0	0.0	16.862867303103613	0.0
15	0.0	0.0	0.0	17.364980065096095	0.0
16	0.0	0.0	0.0	18.466070676352714	0.0
17	0.0	0.0	0.0	19.78495261194467	0.0
18	0.0	0.0	0.0	21.271159506119048	0.0
19	0.0	0.0	0.0	22.158595380397127	0.0
20	0.0	0.0	0.0	22.987084884840854	0.0
21	2.757724904531011E-4	0.0	0.0	24.017646681664093	0.0
22	4.826018582929269E-4	0.0	0.0	25.102535659106593	0.0
23	0.001240976207038955	0.0	0.0	26.13571729458914	0.0
24	0.0015167486974920561	0.0	0.0	26.967722898286144	0.0
25	0.00248195241407791	0.0	0.0	27.67618242626016	0.0
26	0.002757724904531011	0.0	0.0	28.309011348727413	0.0
27	0.0028266680271442864	0.0	0.0	28.925845466748388	0.0
28	0.0028266680271442864	0.0	0.0	29.57487602302976	0.0
29	0.0028266680271442864	0.0	0.0	30.262032126116274	0.0
30	0.0028266680271442864	0.0	0.0	30.997517358154695	0.0
31	0.0028266680271442864	0.0	0.0	31.690464683540725	0.0
32	0.0028266680271442864	0.0	0.0	32.34907833386534	0.0
33	0.0028956111497575616	0.0	0.0	32.98121782510646	0.0
34	0.0031713836402106625	0.0	0.0	33.626387566521494	0.0
35	0.003516099253277039	0.0	0.0	34.297273092671276	0.0
36	0.003516099253277039	0.0	0.0	34.94340803780289	0.0
37	0.003516099253277039	0.0	0.0	35.57003207923495	0.0
38	0.003516099253277039	0.0	0.0	36.20816962214343	0.0
39	0.003516099253277039	0.0	0.0	36.844790416354414	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTGCG	50	2.1827873E-11	45.000004	1
TACGCTA	20	7.033899E-4	45.0	18
ACTACGT	20	7.033899E-4	45.0	23
CCGTTAG	20	7.033899E-4	45.0	1
TTACGAG	85	0.0	42.35294	1
GCAACGT	45	1.9288564E-8	40.000004	39
CAGTACG	85	0.0	39.705883	1
AATTGAC	40	3.459063E-7	39.375	31
AAGTACG	35	6.24942E-6	38.57143	1
TTAGGCG	35	6.24942E-6	38.57143	1
TAGCACG	155	0.0	37.741936	1
TGCGGGT	275	0.0	37.636364	4
ATTTGCG	90	0.0	37.500004	1
ATTTACG	60	1.5643309E-10	37.499996	1
TTGTCGC	30	1.14006936E-4	37.499996	42
CCTACGG	30	1.14006936E-4	37.499996	2
GGCGAAT	60	1.5643309E-10	37.499996	8
ACTTGCG	55	2.750312E-9	36.81818	1
CTAACGG	80	0.0	36.5625	2
TCAACGG	80	0.0	36.5625	2
>>END_MODULE