FastQCFastQC Report
Sat 18 Jun 2016
SRR3552805_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552805_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621988
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC52270.3222588576487619TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT42560.2623940497710217TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC34720.21405830376057033TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC27710.17083973494255197TruSeq Adapter, Index 16 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC16310.10055561446817116TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG850.045.0000041
ATCCGTT207.034146E-445.013
GCGGATA253.8915176E-545.01
TATCGCG253.8915176E-545.01
ATCGATC207.034146E-445.030
CGACAGG1500.040.5000042
CCGCTAT501.0822987E-940.55
AGTTGCG950.040.263161
AACGGTT900.040.029
GTGTACG1200.039.3749961
AACACGT3850.039.1558441
CGACCAA4000.038.812529
TACGGGA6400.038.6718754
TCGTAAG700.038.5714261
ATTAGCG659.094947E-1238.0769271
GTTAACG659.094947E-1238.0769271
TCGAGAG659.094947E-1238.0769271
ACACGAC4200.038.03571326
CGCACGG2250.038.02
ACGGGAT7950.037.924535
Produced by FastQC (version 0.11.5)