Basic Statistics
Measure | Value |
---|---|
Filename | SRR3552803_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 988457 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC | 5791 | 0.5858626121318378 | RNA PCR Primer, Index 36 (96% over 25bp) |
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC | 5271 | 0.5332553666977926 | RNA PCR Primer, Index 15 (96% over 25bp) |
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT | 4442 | 0.44938727734236295 | RNA PCR Primer, Index 36 (96% over 26bp) |
TCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC | 2144 | 0.21690371963575553 | RNA PCR Primer, Index 15 (96% over 25bp) |
ACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC | 1384 | 0.14001620707830487 | RNA PCR Primer, Index 15 (96% over 25bp) |
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA | 1013 | 0.10248296081670726 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTTGACG | 85 | 0.0 | 45.000004 | 1 |
ATCCGGC | 30 | 2.1651922E-6 | 45.000004 | 12 |
CGTGTCG | 30 | 2.1651922E-6 | 45.000004 | 39 |
AGTTACG | 30 | 2.1651922E-6 | 45.000004 | 1 |
TTATTCG | 30 | 2.1651922E-6 | 45.000004 | 27 |
ACGAACG | 30 | 2.1651922E-6 | 45.000004 | 1 |
ACGCGAA | 20 | 7.0328073E-4 | 45.0 | 43 |
CGTATCG | 20 | 7.0328073E-4 | 45.0 | 40 |
GCGAATG | 50 | 2.1827873E-11 | 45.0 | 1 |
GAATCGC | 20 | 7.0328073E-4 | 45.0 | 37 |
TTATCCG | 40 | 6.8139343E-9 | 45.0 | 1 |
CTGCGAC | 20 | 7.0328073E-4 | 45.0 | 13 |
TCTACCG | 20 | 7.0328073E-4 | 45.0 | 38 |
GTATACG | 20 | 7.0328073E-4 | 45.0 | 1 |
ATGCGAG | 25 | 3.8904087E-5 | 45.0 | 1 |
CCGCGAT | 20 | 7.0328073E-4 | 45.0 | 26 |
CGGTAAT | 20 | 7.0328073E-4 | 45.0 | 6 |
TGTCGTA | 20 | 7.0328073E-4 | 45.0 | 37 |
TATCGGC | 25 | 3.8904087E-5 | 45.0 | 15 |
AGTACGG | 45 | 3.8380676E-10 | 45.0 | 2 |