FastQCFastQC Report
Sat 18 Jun 2016
SRR3552803_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552803_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988457
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC57910.5858626121318378RNA PCR Primer, Index 36 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC52710.5332553666977926RNA PCR Primer, Index 15 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT44420.44938727734236295RNA PCR Primer, Index 36 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC21440.21690371963575553RNA PCR Primer, Index 15 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC13840.14001620707830487RNA PCR Primer, Index 15 (96% over 25bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA10130.10248296081670726No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACG850.045.0000041
ATCCGGC302.1651922E-645.00000412
CGTGTCG302.1651922E-645.00000439
AGTTACG302.1651922E-645.0000041
TTATTCG302.1651922E-645.00000427
ACGAACG302.1651922E-645.0000041
ACGCGAA207.0328073E-445.043
CGTATCG207.0328073E-445.040
GCGAATG502.1827873E-1145.01
GAATCGC207.0328073E-445.037
TTATCCG406.8139343E-945.01
CTGCGAC207.0328073E-445.013
TCTACCG207.0328073E-445.038
GTATACG207.0328073E-445.01
ATGCGAG253.8904087E-545.01
CCGCGAT207.0328073E-445.026
CGGTAAT207.0328073E-445.06
TGTCGTA207.0328073E-445.037
TATCGGC253.8904087E-545.015
AGTACGG453.8380676E-1045.02