##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552803_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	988457
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.692198041998793	31.0	31.0	34.0	30.0	34.0
2	31.86949558756729	33.0	31.0	34.0	30.0	34.0
3	31.91748958224789	33.0	31.0	34.0	30.0	34.0
4	35.51896845285126	37.0	35.0	37.0	33.0	37.0
5	35.39860307529817	37.0	35.0	37.0	33.0	37.0
6	35.36965391514249	37.0	35.0	37.0	33.0	37.0
7	35.747500397083535	37.0	35.0	37.0	35.0	37.0
8	35.73265200205977	37.0	35.0	37.0	35.0	37.0
9	37.33312931164431	39.0	37.0	39.0	35.0	39.0
10	36.802436524805834	39.0	37.0	39.0	32.0	39.0
11	36.59834975117784	39.0	35.0	39.0	32.0	39.0
12	36.38007014973843	38.0	35.0	39.0	32.0	39.0
13	36.23852327415356	38.0	35.0	39.0	32.0	39.0
14	37.287147544101565	39.0	36.0	41.0	32.0	41.0
15	37.37656569785029	39.0	36.0	41.0	32.0	41.0
16	37.456623808622936	39.0	36.0	41.0	32.0	41.0
17	37.441029807062925	39.0	36.0	41.0	32.0	41.0
18	37.41625685285248	39.0	36.0	41.0	32.0	41.0
19	37.40726202556105	39.0	36.0	41.0	32.0	41.0
20	37.350946981001705	39.0	35.0	41.0	32.0	41.0
21	37.28172191607728	39.0	35.0	41.0	32.0	41.0
22	37.220164357174866	39.0	35.0	41.0	32.0	41.0
23	37.20028590014538	39.0	35.0	40.0	32.0	41.0
24	37.19839203930975	39.0	35.0	41.0	32.0	41.0
25	37.15842064955785	39.0	35.0	41.0	32.0	41.0
26	37.04481833807642	39.0	35.0	40.0	31.0	41.0
27	36.9735608124582	39.0	35.0	40.0	31.0	41.0
28	36.877279436535936	39.0	35.0	40.0	31.0	41.0
29	36.87194283615777	39.0	35.0	40.0	31.0	41.0
30	36.736003690600604	39.0	35.0	40.0	31.0	41.0
31	36.581384926203164	38.0	35.0	40.0	30.0	41.0
32	36.53517249612275	38.0	35.0	40.0	30.0	41.0
33	36.44861132047221	38.0	35.0	40.0	30.0	41.0
34	36.24442034403115	38.0	35.0	40.0	30.0	41.0
35	36.28202339606073	38.0	35.0	40.0	30.0	41.0
36	36.19240290675265	38.0	35.0	40.0	30.0	41.0
37	36.1220123890063	38.0	35.0	40.0	30.0	41.0
38	36.04461397916146	38.0	35.0	40.0	30.0	41.0
39	35.96079748537367	38.0	34.0	40.0	29.0	41.0
40	35.84331538954148	38.0	34.0	40.0	29.0	41.0
41	35.74809425195026	38.0	34.0	40.0	28.0	41.0
42	35.7027205027634	38.0	34.0	40.0	28.0	41.0
43	35.58245831634558	38.0	34.0	40.0	28.0	41.0
44	35.411910685037384	38.0	34.0	40.0	28.0	41.0
45	35.28726995711498	37.0	34.0	40.0	27.0	41.0
46	35.10309097917259	37.0	34.0	40.0	26.0	41.0
47	34.96965877119592	37.0	33.0	40.0	26.0	41.0
48	34.925209695515335	37.0	33.0	40.0	26.0	41.0
49	34.85425567323617	37.0	33.0	40.0	26.0	41.0
50	34.66024824549778	36.0	33.0	40.0	26.0	41.0
51	32.9264570942388	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	3.0
11	7.0
12	10.0
13	6.0
14	15.0
15	36.0
16	97.0
17	162.0
18	348.0
19	631.0
20	1057.0
21	1573.0
22	2343.0
23	3572.0
24	5043.0
25	6720.0
26	8770.0
27	11292.0
28	14551.0
29	18367.0
30	23283.0
31	29811.0
32	38323.0
33	51238.0
34	81619.0
35	91852.0
36	90115.0
37	124755.0
38	187884.0
39	194943.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08563751382205	21.232183089400955	24.90568633739252	10.776493059384475
2	30.457875254057587	25.17863700697147	25.78564368505661	18.577844053914333
3	32.47657712980939	24.19650020182972	25.951356508173852	17.375566160187038
4	29.669373579224995	27.115696484520825	25.370754620585416	17.844175315668764
5	25.447338629803824	30.124122748890443	24.28289748567717	20.145641135628562
6	26.441312065168237	34.5264386817029	23.788996385275233	15.243252867853634
7	80.05598624927539	6.63043511250363	8.35696444053712	4.956614197683865
8	80.68241714105925	4.887617771941521	8.061251020529978	6.368714066469254
9	75.4250311343842	6.675657110020971	10.844680142889372	7.0546316127054585
10	42.89695960471725	24.58862651587272	17.527317829708323	14.987096049701707
11	32.01818592007543	25.419213987052547	21.789314052103432	20.773286040768593
12	28.509282649624616	22.46754284708389	29.91207508267937	19.111099420612128
13	24.47400342149431	25.747908103235652	29.562742739441372	20.21534573582867
14	20.889527819621893	28.240985697910986	28.743081388467072	22.12640509400004
15	19.954332864252063	26.20781682966482	33.36978745661167	20.46806284947145
16	22.128833120712383	25.66049914159139	29.931195793039052	22.27947194465718
17	21.745913074620344	25.645020471300217	27.610710430499253	24.998356023580186
18	23.779891285103954	24.291800250289086	30.04146867289118	21.886839791715776
19	24.058001511446626	27.00947031585593	26.50454192746877	22.427986245228674
20	26.182929555863332	26.43979454847302	27.00673878580454	20.370537109859104
21	24.595101253772295	27.737170155100323	27.032030730724756	20.635697860402626
22	22.732400094288373	25.78068646385225	25.85302142632406	25.633892015535327
23	22.346748518144945	26.735609136259846	26.64941418797176	24.268228157623447
24	24.054258303598434	23.79344776758119	28.084074471625982	24.068219457194395
25	21.975260431156844	25.574000689964254	26.031177886342043	26.41956099253685
26	22.684648902279005	27.227992719966576	26.82322043346347	23.26413794429095
27	24.24708409167015	26.6877567764708	25.884585773584483	23.180573358274565
28	20.66766687878178	27.744251899677984	27.06096471571348	24.527116505826758
29	23.57866857131873	25.630452311026175	25.771986034799692	25.0188930828554
30	25.19613903285626	27.141393100559764	24.739973514275277	22.9224943523087
31	24.230189072463446	29.711864046690955	23.11744466375371	22.94050221709189
32	24.665918699548893	27.877489865517667	23.894514379482366	23.562077055451073
33	24.570517483309846	28.030657883954486	22.81242380801593	24.58640082471974
34	20.916236113457643	28.017809575935015	26.91093289844677	24.15502141216057
35	23.100751980106367	25.227905715676048	26.156120094247903	25.51522220996968
36	24.195994362931316	30.03408342497448	23.80882527009268	21.961096942001525
37	23.340823121289038	29.18417290787561	25.1843024026336	22.290701568201754
38	20.94486659510732	29.826588308849043	24.15866345222908	25.06988164381455
39	22.542609339607083	26.786193026100275	25.48011699042042	25.191080643872215
40	23.89744824509311	25.671829932915646	27.548492245995526	22.88222957599572
41	20.39036599467655	25.97159006411002	26.630192309832395	27.00785163138103
42	22.781365299653906	24.643864123578467	25.954290373784595	26.620480202983032
43	23.712614711616187	23.752980655708846	26.38182541071589	26.152579221959073
44	21.38777913455011	25.76551129690012	25.558016180774683	27.288693387775087
45	23.427220405136488	24.760510573550494	24.677047155313787	27.13522186599923
46	22.96174745082487	26.43534316616707	26.549156918308032	24.053752464700032
47	20.031625047928237	26.04321685212407	29.001868568890703	24.92328953105699
48	20.601199647531455	23.64796850040012	29.385597957220195	26.365233894848235
49	22.03646693786376	22.788447044231567	29.35909199894381	25.815994018960865
50	20.57671704484869	23.946514618238325	28.428955432558016	27.047812904354966
51	20.338669259259635	23.43288580079862	26.47985698922664	29.748587950715105
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	328.0
1	591.5
2	855.0
3	1142.5
4	1430.0
5	1128.0
6	826.0
7	873.5
8	921.0
9	978.5
10	1036.0
11	1051.0
12	1066.0
13	1158.0
14	1250.0
15	1502.0
16	1754.0
17	1708.5
18	1663.0
19	1828.5
20	1994.0
21	2123.0
22	2252.0
23	2829.5
24	3407.0
25	4310.0
26	6009.5
27	6806.0
28	8467.0
29	10128.0
30	11792.0
31	13456.0
32	14659.5
33	15863.0
34	19205.0
35	22547.0
36	23306.0
37	24065.0
38	26590.5
39	29116.0
40	33121.0
41	37126.0
42	43183.0
43	49240.0
44	56530.0
45	63820.0
46	74791.5
47	85763.0
48	98030.5
49	110298.0
50	110610.0
51	110922.0
52	94801.5
53	78681.0
54	70028.5
55	61376.0
56	56401.5
57	51427.0
58	48585.5
59	45744.0
60	43915.0
61	42086.0
62	37778.5
63	33471.0
64	29367.0
65	25263.0
66	20631.0
67	15999.0
68	13818.5
69	11638.0
70	9777.5
71	7917.0
72	6218.0
73	4519.0
74	3927.5
75	2583.5
76	1831.0
77	1438.5
78	1046.0
79	819.0
80	592.0
81	388.5
82	185.0
83	127.0
84	69.0
85	63.0
86	57.0
87	40.0
88	23.0
89	15.5
90	8.0
91	5.0
92	2.0
93	2.5
94	3.0
95	21.0
96	39.0
97	19.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	988457.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.768339744226346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.1115697904157	15.951305915469577
2	12.992454572750859	6.17618148793139
3	5.729882243550994	4.085693635773898
4	3.032457444547829	2.8830591520768487
5	1.7329455336193493	2.059461910065216
6	1.1417618919305152	1.628267073264908
7	0.7667707977904975	1.2757408179485217
8	0.5652952523026882	1.0748903690022753
9	0.4387886713694251	0.938635039532356
>10	4.601899215378479	27.957793881589797
>50	1.7370749421963667	27.481092176998896
>100	0.14055525193253138	5.400564908879386
>500	0.005981074550320483	1.0518840254034327
>1k	0.0017088784429487094	0.9121608456606849
>5k	8.544392214743547E-4	1.123268760402816
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	5791	0.5858626121318378	RNA PCR Primer, Index 36 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	5271	0.5332553666977926	RNA PCR Primer, Index 15 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT	4442	0.44938727734236295	RNA PCR Primer, Index 36 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	2144	0.21690371963575553	RNA PCR Primer, Index 15 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	1384	0.14001620707830487	RNA PCR Primer, Index 15 (96% over 25bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1013	0.10248296081670726	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0233555936171224E-4	0.0	0.0	0.4832784835354497	0.0
2	2.0233555936171224E-4	0.0	0.0	2.097005737224786	0.0
3	2.0233555936171224E-4	0.0	0.0	2.7975926115147143	0.0
4	2.0233555936171224E-4	0.0	0.0	3.550382060119965	0.0
5	2.0233555936171224E-4	0.0	0.0	5.321829882331755	0.0
6	2.0233555936171224E-4	0.0	0.0	6.235476100629567	0.0
7	2.0233555936171224E-4	0.0	0.0	7.2748738690706825	0.0
8	2.0233555936171224E-4	0.0	0.0	8.867355889027039	0.0
9	2.0233555936171224E-4	0.0	0.0	9.389078128841215	0.0
10	2.0233555936171224E-4	0.0	0.0	10.695862338978833	0.0
11	2.0233555936171224E-4	0.0	0.0	12.697568027744252	0.0
12	2.0233555936171224E-4	0.0	0.0	14.193839489224114	0.0
13	2.0233555936171224E-4	0.0	0.0	14.79244924159574	0.0
14	2.0233555936171224E-4	0.0	0.0	15.042131321848093	0.0
15	2.0233555936171224E-4	0.0	0.0	15.515495362974818	0.0
16	3.035033390425684E-4	0.0	0.0	16.519787911866676	0.0
17	3.035033390425684E-4	0.0	0.0	17.700011229623545	0.0
18	3.035033390425684E-4	0.0	0.0	19.061122537449783	0.0
19	3.035033390425684E-4	0.0	0.0	19.800052000238757	0.0
20	3.035033390425684E-4	0.0	0.0	20.47757262076145	0.0
21	4.046711187234245E-4	0.0	0.0	21.34144429145628	0.0
22	8.09342237446849E-4	0.0	0.0	22.19105130521611	0.0
23	9.105100171277051E-4	0.0	0.0	23.070705149541155	0.0
24	0.0015175166952128417	0.0	0.0	23.80882527009268	0.0
25	0.0019221878139362664	0.0	0.0	24.445271772064945	0.0
26	0.0020233555936171226	0.0	0.0	24.999367701376993	0.0
27	0.0021245233732979786	0.0	0.0	25.508545136510744	0.0
28	0.0022256911529788346	0.0	0.0	26.05191728117662	0.0
29	0.0022256911529788346	0.0	0.0	26.63160865874793	0.0
30	0.0022256911529788346	0.0	0.0	27.245494745851364	0.0
31	0.0022256911529788346	0.0	0.0	27.827310646795965	0.0
32	0.0022256911529788346	0.0	0.0	28.389398830702802	0.0
33	0.002428026712340547	0.0	0.0	28.960592114780916	0.0
34	0.002529194492021403	0.0	0.0	29.502547910531263	0.0
35	0.002529194492021403	0.0	0.0	30.086387167069482	0.0
36	0.002630362271702259	0.0	0.0	30.674981309252704	0.0
37	0.002630362271702259	0.0	0.0	31.21430674273135	0.0
38	0.002630362271702259	0.0	0.0	31.809577958373506	0.0
39	0.002630362271702259	0.0	0.0	32.369238115568	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACG	85	0.0	45.000004	1
ATCCGGC	30	2.1651922E-6	45.000004	12
CGTGTCG	30	2.1651922E-6	45.000004	39
AGTTACG	30	2.1651922E-6	45.000004	1
TTATTCG	30	2.1651922E-6	45.000004	27
ACGAACG	30	2.1651922E-6	45.000004	1
ACGCGAA	20	7.0328073E-4	45.0	43
CGTATCG	20	7.0328073E-4	45.0	40
GCGAATG	50	2.1827873E-11	45.0	1
GAATCGC	20	7.0328073E-4	45.0	37
TTATCCG	40	6.8139343E-9	45.0	1
CTGCGAC	20	7.0328073E-4	45.0	13
TCTACCG	20	7.0328073E-4	45.0	38
GTATACG	20	7.0328073E-4	45.0	1
ATGCGAG	25	3.8904087E-5	45.0	1
CCGCGAT	20	7.0328073E-4	45.0	26
CGGTAAT	20	7.0328073E-4	45.0	6
TGTCGTA	20	7.0328073E-4	45.0	37
TATCGGC	25	3.8904087E-5	45.0	15
AGTACGG	45	3.8380676E-10	45.0	2
>>END_MODULE