FastQCFastQC Report
Sat 18 Jun 2016
SRR3552802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618896
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC49050.7925402652465035RNA PCR Primer, Index 36 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT31080.5021845350430444RNA PCR Primer, Index 36 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC31070.5020229570073163RNA PCR Primer, Index 15 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC12700.2052041053747318RNA PCR Primer, Index 15 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC8480.13701817429745872RNA PCR Primer, Index 15 (96% over 25bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA7190.11617460768852926No Hit
GCCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTG7070.11423567125979162RNA PCR Primer, Index 15 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGT207.03076E-445.029
GATACCG207.03076E-445.01
GCGAATG207.03076E-445.01
CCTCGAA253.8887112E-545.023
TCGTCGG207.03076E-445.010
AGTTACC207.03076E-445.017
TGTAGCG502.1827873E-1145.01
ACATAAC207.03076E-445.010
GTGGTCG207.03076E-445.013
CTAGGTA253.8887112E-545.027
TTCGACA253.8887112E-545.019
AGTACGG302.1638716E-644.9999962
GCTACGA1000.042.7510
TCGTTCG603.6379788E-1241.24999645
TACGAAT1000.040.512
GGTATTA501.0804797E-940.58
CGAATAT1000.040.514
GGCCGAT900.040.08
ACGTTAG403.4547702E-739.3751
CGTACAC403.4547702E-739.37525