Basic Statistics
Measure | Value |
---|---|
Filename | SRR3552802_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 618896 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC | 4905 | 0.7925402652465035 | RNA PCR Primer, Index 36 (96% over 25bp) |
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT | 3108 | 0.5021845350430444 | RNA PCR Primer, Index 36 (96% over 26bp) |
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC | 3107 | 0.5020229570073163 | RNA PCR Primer, Index 15 (96% over 25bp) |
TCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC | 1270 | 0.2052041053747318 | RNA PCR Primer, Index 15 (96% over 25bp) |
ACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC | 848 | 0.13701817429745872 | RNA PCR Primer, Index 15 (96% over 25bp) |
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA | 719 | 0.11617460768852926 | No Hit |
GCCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTG | 707 | 0.11423567125979162 | RNA PCR Primer, Index 15 (95% over 24bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCTTCGT | 20 | 7.03076E-4 | 45.0 | 29 |
GATACCG | 20 | 7.03076E-4 | 45.0 | 1 |
GCGAATG | 20 | 7.03076E-4 | 45.0 | 1 |
CCTCGAA | 25 | 3.8887112E-5 | 45.0 | 23 |
TCGTCGG | 20 | 7.03076E-4 | 45.0 | 10 |
AGTTACC | 20 | 7.03076E-4 | 45.0 | 17 |
TGTAGCG | 50 | 2.1827873E-11 | 45.0 | 1 |
ACATAAC | 20 | 7.03076E-4 | 45.0 | 10 |
GTGGTCG | 20 | 7.03076E-4 | 45.0 | 13 |
CTAGGTA | 25 | 3.8887112E-5 | 45.0 | 27 |
TTCGACA | 25 | 3.8887112E-5 | 45.0 | 19 |
AGTACGG | 30 | 2.1638716E-6 | 44.999996 | 2 |
GCTACGA | 100 | 0.0 | 42.75 | 10 |
TCGTTCG | 60 | 3.6379788E-12 | 41.249996 | 45 |
TACGAAT | 100 | 0.0 | 40.5 | 12 |
GGTATTA | 50 | 1.0804797E-9 | 40.5 | 8 |
CGAATAT | 100 | 0.0 | 40.5 | 14 |
GGCCGAT | 90 | 0.0 | 40.0 | 8 |
ACGTTAG | 40 | 3.4547702E-7 | 39.375 | 1 |
CGTACAC | 40 | 3.4547702E-7 | 39.375 | 25 |