##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552802_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	618896
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53631951087097	33.0	33.0	33.0	27.0	33.0
2	31.74604780124609	33.0	33.0	33.0	27.0	33.0
3	31.789717496962332	33.0	33.0	33.0	27.0	33.0
4	31.70591828029265	33.0	33.0	33.0	27.0	33.0
5	31.78151741164913	33.0	33.0	33.0	27.0	33.0
6	35.180133657351156	37.0	37.0	37.0	33.0	37.0
7	35.374077389415994	37.0	37.0	37.0	33.0	37.0
8	35.42965377058504	37.0	37.0	37.0	33.0	37.0
9	35.32677865101729	37.0	37.0	37.0	33.0	37.0
10	35.42032587058246	37.0	37.0	37.0	33.0	37.0
11	35.421615263307565	37.0	37.0	37.0	33.0	37.0
12	35.440736408055635	37.0	37.0	37.0	33.0	37.0
13	35.44773435278302	37.0	37.0	37.0	33.0	37.0
14	35.54389590496626	37.0	37.0	37.0	33.0	37.0
15	35.58629236576097	37.0	37.0	37.0	33.0	37.0
16	35.6073023577467	37.0	37.0	37.0	33.0	37.0
17	35.60538765802332	37.0	37.0	37.0	33.0	37.0
18	35.598318618960214	37.0	37.0	37.0	33.0	37.0
19	35.585610506450195	37.0	37.0	37.0	33.0	37.0
20	35.571136346009666	37.0	37.0	37.0	33.0	37.0
21	35.47719164447662	37.0	37.0	37.0	33.0	37.0
22	35.532467490499215	37.0	37.0	37.0	33.0	37.0
23	35.55837168118715	37.0	37.0	37.0	33.0	37.0
24	35.56298473410718	37.0	37.0	37.0	33.0	37.0
25	35.55982911506941	37.0	37.0	37.0	33.0	37.0
26	35.51274850701895	37.0	37.0	37.0	33.0	37.0
27	35.493528799669086	37.0	37.0	37.0	33.0	37.0
28	35.46997880096171	37.0	37.0	37.0	33.0	37.0
29	35.4662156485096	37.0	37.0	37.0	33.0	37.0
30	35.432996820144254	37.0	37.0	37.0	33.0	37.0
31	35.40089449600579	37.0	37.0	37.0	33.0	37.0
32	35.391535896176414	37.0	37.0	37.0	33.0	37.0
33	35.376612548796565	37.0	37.0	37.0	33.0	37.0
34	35.33846397456115	37.0	37.0	37.0	33.0	37.0
35	35.33607100385202	37.0	37.0	37.0	33.0	37.0
36	35.308145471937124	37.0	37.0	37.0	33.0	37.0
37	35.29679138336651	37.0	37.0	37.0	33.0	37.0
38	35.27446776195031	37.0	37.0	37.0	33.0	37.0
39	35.271060081176806	37.0	37.0	37.0	33.0	37.0
40	35.2659202838603	37.0	37.0	37.0	33.0	37.0
41	35.26683643132287	37.0	37.0	37.0	33.0	37.0
42	35.25613188645588	37.0	37.0	37.0	33.0	37.0
43	35.24307153382798	37.0	37.0	37.0	33.0	37.0
44	35.22065581293141	37.0	37.0	37.0	33.0	37.0
45	35.170248313125306	37.0	37.0	37.0	33.0	37.0
46	35.177506398490216	37.0	37.0	37.0	33.0	37.0
47	35.15324222486492	37.0	37.0	37.0	33.0	37.0
48	35.16140191566919	37.0	37.0	37.0	33.0	37.0
49	35.156454396215196	37.0	37.0	37.0	33.0	37.0
50	35.137607934127864	37.0	37.0	37.0	33.0	37.0
51	34.742766151340454	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	486.0
15	1862.0
16	1819.0
17	1379.0
18	1268.0
19	1315.0
20	1405.0
21	1603.0
22	1795.0
23	2188.0
24	2599.0
25	3309.0
26	4239.0
27	5614.0
28	7040.0
29	8527.0
30	10383.0
31	12689.0
32	16502.0
33	22319.0
34	34081.0
35	70977.0
36	405497.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.597793490344095	19.535430831674468	23.215855329489933	13.650920348491507
2	29.605943486466224	24.64662883586257	25.661338900235258	20.08608877743595
3	32.602731315115946	23.844717044543835	25.195186267159585	18.35736537318063
4	30.509164706186496	26.61691140353145	24.470831932990357	18.403091957291693
5	25.853132028644556	29.528547607352447	24.025846022595072	20.592474341407925
6	26.238818799927614	34.3138427134769	23.3598213593237	16.087517127271784
7	78.70999327835372	7.346306972415398	8.652826969313098	5.290872779917789
8	79.63470437682582	5.4988237118999	8.109924769266565	6.756547142007705
9	74.69542540265246	7.102970450608826	11.032709857552804	7.168894289185905
10	42.650946200977224	24.718046327654402	17.524592177037825	15.10641529433055
11	32.09634575114397	25.570370466120316	21.60928492024508	20.723998862490628
12	28.22881388795533	22.698159303016986	29.91278017631395	19.160246632713733
13	24.762965021586826	25.58830562808614	29.25515757090044	20.39357177942659
14	21.265446860215608	28.12007186991029	28.487661901191803	22.1268193686823
15	20.128745378868178	25.89756598846979	33.224483596597814	20.749205036064218
16	22.356906491559165	25.37631524521083	29.754756857371838	22.512021405858174
17	22.282903751195676	25.301989348775884	27.635660918797345	24.779445981231095
18	23.930999715622658	24.014050825986917	30.137696802047515	21.91725265634291
19	24.18112251492981	26.658760114785036	26.648580698534165	22.51153667175099
20	26.28939272511052	26.078048654378115	27.066744654998576	20.565813965512785
21	24.9793180114268	27.41736899252863	26.967858897132956	20.63545409891161
22	22.877672500710943	25.771858276673303	25.95977353222512	25.39069569039063
23	22.08787906207182	26.66797006282154	26.596067836922522	24.648083038184122
24	23.828882397042474	23.560339701662315	28.10132881776583	24.50944908352938
25	21.808510638297875	25.46486000878985	26.25142188671441	26.475207466197876
26	22.70106770766009	26.83552648587162	26.981916186241307	23.481489620226984
27	24.079813086528272	26.465351206018457	26.17774230242238	23.277093405030893
28	20.752275018743052	27.38909283627621	27.35338409038029	24.50524805460045
29	23.28258705824565	25.54419482433236	26.096145394379672	25.07707272304232
30	24.804490576768956	27.160621493756626	24.99660686124971	23.03828106822471
31	24.093385641529434	29.575728393785063	23.427522556293788	22.903363408391716
32	24.61528269693131	27.410744293063775	24.26885938833019	23.705113621674723
33	24.52496057495928	27.89660944649828	23.016306455365683	24.562123523176755
34	21.081086321449806	27.457117189317753	27.20537860965332	24.25641787957912
35	23.240092034849148	25.107934127866393	26.134600966882964	25.517372870401488
36	24.462591453168223	29.525477624673613	24.049597993847108	21.962332928311056
37	23.316518445748557	29.159826529820844	25.16125487965668	22.36240014477392
38	20.87976008893255	29.905024430599003	24.253347896900287	24.96186758356816
39	22.199206328688504	26.969151521418784	25.68880716630904	25.142834983583672
40	23.786225795610246	25.794156045603785	27.483777565212897	22.935840593573072
41	20.39292546728368	25.921641115793282	26.559874356919416	27.125559060003617
42	22.801246089811535	24.73743569194178	25.863957757038342	26.597360461208346
43	23.653247072205993	23.491184302370673	26.59784519531553	26.257723430107806
44	21.353345311651715	25.51995811897314	26.016002688658514	27.11069388071663
45	23.23314417931284	24.777183888730903	24.89158113802642	27.098090793929835
46	22.744209043199504	26.596390992993978	26.91744654998578	23.741953413820738
47	19.89300302474083	26.30118792171867	28.979505441948245	24.826303611592255
48	20.457233525503476	24.027300224916626	29.23189033375559	26.283575915824308
49	22.08594012564308	22.812233396241048	29.357113311444895	25.744713166670973
50	20.658721336056463	24.208429202967867	28.206192962953384	26.926656498022282
51	20.416515860499988	23.570680695948916	26.348853442258473	29.663950001292623
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	231.0
1	263.5
2	296.0
3	705.0
4	1114.0
5	905.0
6	696.0
7	694.5
8	693.0
9	769.0
10	845.0
11	829.0
12	813.0
13	870.0
14	927.0
15	1031.0
16	1135.0
17	1186.0
18	1237.0
19	1301.0
20	1365.0
21	1430.0
22	1495.0
23	1838.0
24	2181.0
25	2655.0
26	3460.5
27	3792.0
28	4912.0
29	6032.0
30	7061.5
31	8091.0
32	8799.0
33	9507.0
34	11793.0
35	14079.0
36	14430.0
37	14781.0
38	16392.0
39	18003.0
40	20320.0
41	22637.0
42	26347.5
43	30058.0
44	34065.5
45	38073.0
46	44915.5
47	51758.0
48	59722.0
49	67686.0
50	68903.0
51	70120.0
52	60272.5
53	50425.0
54	44891.5
55	39358.0
56	36523.0
57	33688.0
58	31410.0
59	29132.0
60	27802.5
61	26473.0
62	24102.0
63	21731.0
64	19129.5
65	16528.0
66	13508.5
67	10489.0
68	9171.0
69	7853.0
70	6512.0
71	5171.0
72	4022.0
73	2873.0
74	2478.5
75	1675.0
76	1266.0
77	952.0
78	638.0
79	462.0
80	286.0
81	185.0
82	84.0
83	59.5
84	35.0
85	20.5
86	6.0
87	3.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	618896.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.192237709417263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.03357163752409	19.744030992445822
2	12.131336841442945	6.840190639339413
3	4.8083912654765415	4.06677928668601
4	2.2989627218307955	2.592516141557708
5	1.368893038035784	1.929607896353559
6	0.9176565448820235	1.5522474869349923
7	0.575655147387783	1.1360304727664206
8	0.5083822976616257	1.146594766634902
9	0.40058536880125767	1.0164058146143673
>10	6.4221222805251195	46.25381773281673
>50	0.4695749264891993	7.930721063815597
>100	0.05797930021443626	2.9794419799372385
>500	0.004592419818965248	0.8064399449227192
>1k	0.002296209909482624	2.0051757811744912
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	4905	0.7925402652465035	RNA PCR Primer, Index 36 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT	3108	0.5021845350430444	RNA PCR Primer, Index 36 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	3107	0.5020229570073163	RNA PCR Primer, Index 15 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	1270	0.2052041053747318	RNA PCR Primer, Index 15 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	848	0.13701817429745872	RNA PCR Primer, Index 15 (96% over 25bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	719	0.11617460768852926	No Hit
GCCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTG	707	0.11423567125979162	RNA PCR Primer, Index 15 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5223817895090613	0.0
2	0.0	0.0	0.0	2.2275148005480725	0.0
3	0.0	0.0	0.0	2.8835216256043017	0.0
4	0.0	0.0	0.0	3.587355549236059	0.0
5	0.0	0.0	0.0	5.1396357384762545	0.0
6	0.0	0.0	0.0	5.933630206044311	0.0
7	0.0	0.0	0.0	6.855594477909051	0.0
8	0.0	0.0	0.0	8.3564928517877	0.0
9	0.0	0.0	0.0	8.894062976655205	0.0
10	0.0	0.0	0.0	10.222396008376206	0.0
11	0.0	0.0	0.0	12.228387321941005	0.0
12	0.0	0.0	0.0	13.75126030867868	0.0
13	0.0	0.0	0.0	14.336819110157442	0.0
14	0.0	0.0	0.0	14.593728186965176	0.0
15	0.0	0.0	0.0	15.063435536826866	0.0
16	0.0	0.0	0.0	16.00995967012228	0.0
17	0.0	0.0	0.0	17.156355833613404	0.0
18	0.0	0.0	0.0	18.511672397301002	0.0
19	0.0	0.0	0.0	19.252830847185958	0.0
20	0.0	0.0	0.0	19.952302163853055	0.0
21	1.6157803572813527E-4	0.0	0.0	20.77828908249528	0.0
22	3.2315607145627054E-4	0.0	0.0	21.648709960962748	0.0
23	4.847341071844058E-4	0.0	0.0	22.48972363692769	0.0
24	9.694682143688116E-4	0.0	0.0	23.23589100592022	0.0
25	0.0016157803572813525	0.0	0.0	23.858451177580726	0.0
26	0.001777358393009488	0.0	0.0	24.435769499237352	0.0
27	0.001777358393009488	0.0	0.0	24.952819213567384	0.0
28	0.0019389364287376231	0.0	0.0	25.496044569685374	0.0
29	0.0019389364287376231	0.0	0.0	26.060759804555207	0.0
30	0.0019389364287376231	0.0	0.0	26.670878467464647	0.0
31	0.0019389364287376231	0.0	0.0	27.23898684108477	0.0
32	0.0021005144644657583	0.0	0.0	27.780919572916936	0.0
33	0.0024236705359220287	0.0	0.0	28.33093120653551	0.0
34	0.0024236705359220287	0.0	0.0	28.861715053902433	0.0
35	0.0024236705359220287	0.0	0.0	29.423198728057702	0.0
36	0.0025852485716501643	0.0	0.0	29.98225873167705	0.0
37	0.0025852485716501643	0.0	0.0	30.527907758330965	0.0
38	0.0025852485716501643	0.0	0.0	31.08292831105711	0.0
39	0.0025852485716501643	0.0	0.0	31.631647320389856	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGT	20	7.03076E-4	45.0	29
GATACCG	20	7.03076E-4	45.0	1
GCGAATG	20	7.03076E-4	45.0	1
CCTCGAA	25	3.8887112E-5	45.0	23
TCGTCGG	20	7.03076E-4	45.0	10
AGTTACC	20	7.03076E-4	45.0	17
TGTAGCG	50	2.1827873E-11	45.0	1
ACATAAC	20	7.03076E-4	45.0	10
GTGGTCG	20	7.03076E-4	45.0	13
CTAGGTA	25	3.8887112E-5	45.0	27
TTCGACA	25	3.8887112E-5	45.0	19
AGTACGG	30	2.1638716E-6	44.999996	2
GCTACGA	100	0.0	42.75	10
TCGTTCG	60	3.6379788E-12	41.249996	45
TACGAAT	100	0.0	40.5	12
GGTATTA	50	1.0804797E-9	40.5	8
CGAATAT	100	0.0	40.5	14
GGCCGAT	90	0.0	40.0	8
ACGTTAG	40	3.4547702E-7	39.375	1
CGTACAC	40	3.4547702E-7	39.375	25
>>END_MODULE