FastQCFastQC Report
Sat 18 Jun 2016
SRR3552801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555196
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC49880.19521007390431105Illumina PCR Primer Index 6 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGCT39300.1538042482846717Illumina PCR Primer Index 6 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC33420.13079231495353Illumina PCR Primer Index 6 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAC302.1665492E-644.99999623
ATTACGG1700.042.352942
CTATCGT403.4604454E-739.37542
TATTACG403.4604454E-739.3751
ACACGAC14250.039.026
ACGACCA13750.038.94545428
AACACGT13000.038.94230741
GCGAGAC14450.038.92733821
CACGACC14500.038.63792827
TTAACGG2400.038.4374962
GACACGA14650.037.93515425
TGCGATG1500.037.5000041
GTAATCG601.5643309E-1037.4999961
CACGTTA301.1403129E-437.49999641
CGTAAGG5050.037.4257432
GTCAAGC14800.037.39864716
GTTAACG2050.037.3170741
CGACCAA14450.037.21453529
TCAAGCG15000.036.89999817
AGACACG15150.036.3861424
Produced by FastQC (version 0.11.5)