##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552801_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2555196
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.825267807244533	31.0	31.0	34.0	30.0	34.0
2	31.99327996756413	33.0	31.0	34.0	30.0	34.0
3	32.03494252495699	33.0	31.0	34.0	30.0	34.0
4	35.6415413925194	37.0	35.0	37.0	33.0	37.0
5	35.52635179453944	37.0	35.0	37.0	33.0	37.0
6	35.49656347301733	37.0	35.0	37.0	33.0	37.0
7	35.85588033168493	37.0	35.0	37.0	35.0	37.0
8	35.834111355841195	37.0	35.0	37.0	35.0	37.0
9	37.45677043952793	39.0	37.0	39.0	35.0	39.0
10	36.86710099734032	39.0	37.0	39.0	32.0	39.0
11	36.67095948803927	39.0	35.0	39.0	32.0	39.0
12	36.44507662034537	38.0	35.0	39.0	32.0	39.0
13	36.369001829996606	38.0	35.0	39.0	32.0	39.0
14	37.320988292091876	39.0	36.0	41.0	32.0	41.0
15	37.44162796122098	39.0	36.0	41.0	32.0	41.0
16	37.52381461148186	39.0	36.0	41.0	32.0	41.0
17	37.50653100584065	39.0	36.0	41.0	32.0	41.0
18	37.490093910604116	39.0	36.0	41.0	32.0	41.0
19	37.45221932094446	39.0	36.0	41.0	32.0	41.0
20	37.4092312292286	39.0	35.0	41.0	32.0	41.0
21	37.32850278413085	39.0	35.0	41.0	32.0	41.0
22	37.29601760491172	39.0	35.0	41.0	32.0	41.0
23	37.23872610946479	39.0	35.0	41.0	32.0	41.0
24	37.214346766353735	39.0	35.0	41.0	32.0	41.0
25	37.15958423541677	39.0	35.0	41.0	32.0	41.0
26	37.05261279369567	39.0	35.0	41.0	31.0	41.0
27	36.975550994913895	39.0	35.0	41.0	31.0	41.0
28	36.87750920085974	39.0	35.0	40.0	31.0	41.0
29	36.81844406456491	39.0	35.0	40.0	31.0	41.0
30	36.70894287561502	39.0	35.0	40.0	31.0	41.0
31	36.569192343757585	39.0	35.0	40.0	30.0	41.0
32	36.43951814263955	38.0	35.0	40.0	30.0	41.0
33	36.34097149494598	38.0	35.0	40.0	30.0	41.0
34	36.165847160061304	38.0	35.0	40.0	30.0	41.0
35	36.09779093267209	38.0	35.0	40.0	29.0	41.0
36	35.99836803125866	38.0	35.0	40.0	29.0	41.0
37	35.860368441403324	38.0	35.0	40.0	29.0	41.0
38	35.76253759007137	38.0	34.0	40.0	28.0	41.0
39	35.69015292760321	38.0	34.0	40.0	28.0	41.0
40	35.538670223340986	38.0	34.0	40.0	28.0	41.0
41	35.401798531306405	38.0	34.0	40.0	27.0	41.0
42	35.30912188340933	38.0	34.0	40.0	27.0	41.0
43	35.254560119849906	38.0	34.0	40.0	27.0	41.0
44	35.09863392084208	37.0	34.0	40.0	26.0	41.0
45	34.985731035897054	37.0	34.0	40.0	26.0	41.0
46	34.83846718607887	37.0	33.0	40.0	26.0	41.0
47	34.68262708614134	37.0	33.0	40.0	26.0	41.0
48	34.57895793512513	36.0	33.0	40.0	25.0	41.0
49	34.49856762455796	36.0	33.0	40.0	25.0	41.0
50	34.37651475659793	36.0	33.0	40.0	25.0	41.0
51	32.735077857041105	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	15.0
10	20.0
11	13.0
12	27.0
13	27.0
14	67.0
15	143.0
16	318.0
17	590.0
18	1165.0
19	2083.0
20	3339.0
21	5120.0
22	7643.0
23	10934.0
24	15210.0
25	20205.0
26	26641.0
27	33651.0
28	41268.0
29	50491.0
30	61030.0
31	75637.0
32	95662.0
33	128087.0
34	199784.0
35	216647.0
36	235641.0
37	325464.0
38	477849.0
39	520339.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.45525900948499	19.921955106379315	25.68166982102351	9.941116063112183
2	31.797012831892346	23.253676038941826	26.17591762040955	18.773393508756275
3	32.803550099483566	24.54203121795745	25.644099317625734	17.010319364933256
4	28.402948345254142	26.761547842122486	25.382319008013475	19.4531848046099
5	26.031975629266796	30.44463125333634	23.667303799786787	19.856089317610078
6	27.42118412834084	35.46549853709852	22.264867352641442	14.848449981919195
7	84.29693847360437	5.475392102993274	6.304056518560612	3.923612904841742
8	85.98170159940763	4.325499883374896	5.826715445703578	3.866083071513888
9	80.8145050320993	6.0805902952258855	7.890353616708855	5.214551055965961
10	44.98954287655428	24.82427962473329	15.222002539139853	14.964174959572574
11	34.14865239300625	26.008298384937984	20.977294892446608	18.865754329609157
12	28.98951782955202	23.63740394083272	26.83477118780712	20.538307041808142
13	26.047316918154223	24.381652131578164	28.64195153718149	20.929079413086118
14	21.75394764237264	26.305966352483335	29.543604482787227	22.396481522356797
15	22.456868279380526	24.868855461577116	31.207586423898597	21.46668983514376
16	26.347528721867132	24.355235371376597	28.314931613856626	20.982304292899645
17	25.887798822477805	24.118932559380962	27.0804666256522	22.91280199248903
18	27.902712746889087	22.99177832150645	27.131812980295837	21.973695951308628
19	26.389638994425475	25.78851094006096	25.58269502613498	22.239155039378584
20	28.24534791068865	26.277475387406678	25.120029931167707	20.35714677073696
21	26.727616981241358	26.348428848511034	26.76863144745061	20.155322722797
22	25.577372538153632	23.2454966272646	26.609113351774187	24.56801748280758
23	24.270975690318863	26.126293247171645	26.9026720455104	22.700059016999088
24	24.304906551199988	24.76397113959164	27.672084646344153	23.25903766286422
25	24.85194873504811	25.669537679301314	25.39640794678764	24.08210563886293
26	24.806433635619342	25.39092891504213	25.608524747220958	24.194112702117568
27	24.602339703099098	25.186834982521887	26.31938998025983	23.891435334119183
28	21.88419205415162	26.990180009674404	26.325260371415737	24.80036756475824
29	24.83836856350746	25.967244782787702	25.429243001319662	23.765143652385177
30	26.18186628344753	24.476948148008997	26.61169632388279	22.729489244660684
31	25.969866890837334	26.23963093242162	24.163273580578554	23.627228596162485
32	26.23192115203687	26.37586314317962	24.326235639066436	23.065980065717074
33	25.515616023193523	25.529704961967692	24.286121299501097	24.668557715337688
34	23.042224549506184	26.730160817408922	26.984035666931227	23.24357896615367
35	24.112749080696744	27.526733761323985	25.31336147990213	23.047155678077143
36	24.868894597518153	28.748831792159972	23.709100984816818	22.67317262550505
37	23.91659974420749	28.25955425728594	24.057371724126057	23.766474274380517
38	23.199590168425434	28.89437835688534	24.525124491428446	23.380906983260775
39	23.89585769545663	25.26937268217389	25.318175200649968	25.516594421719507
40	25.45119826424274	25.3513233427103	26.953627040743644	22.243851352303306
41	22.624683194557285	26.334065958149587	25.135018996585778	25.906231850707346
42	24.045826621519446	25.061052068021393	25.574867837927112	25.31825347253205
43	23.949826158149904	23.491779104225273	26.71262791582329	25.845766821801536
44	23.43256642543273	24.008960565060374	26.29712162980844	26.261351379698468
45	24.3782081687667	23.902863028902672	25.834887030192593	25.884041772138026
46	23.857621881061178	25.150047197944893	25.810701018630272	25.181629902363657
47	21.570713166426373	26.8006446472208	27.784169981480872	23.844472204871956
48	22.08182855640037	26.1361946402546	27.281351411007222	24.500625392337806
49	23.588836238002877	24.80713808255805	27.464898974481798	24.139126704957274
50	21.814490943160525	25.162844650664763	27.052875787219456	25.96978861895526
51	21.874486340773856	24.62773892883364	25.75735873099363	27.74041599939887
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	596.0
1	1104.0
2	1612.0
3	2785.5
4	3959.0
5	3107.0
6	2255.0
7	2313.5
8	2372.0
9	2681.0
10	2990.0
11	3118.0
12	3246.0
13	3551.0
14	3856.0
15	4135.5
16	4415.0
17	4296.0
18	4177.0
19	4589.5
20	5002.0
21	5883.5
22	6765.0
23	7470.5
24	8176.0
25	10670.0
26	16107.0
27	19050.0
28	22672.5
29	26295.0
30	29816.5
31	33338.0
32	38057.5
33	42777.0
34	49152.5
35	55528.0
36	61231.5
37	66935.0
38	72677.5
39	78420.0
40	83193.0
41	87966.0
42	102205.5
43	116445.0
44	129494.0
45	142543.0
46	162980.5
47	183418.0
48	204929.0
49	226440.0
50	229819.0
51	233198.0
52	216753.5
53	200309.0
54	185015.5
55	169722.0
56	160611.5
57	151501.0
58	144681.0
59	137861.0
60	132652.5
61	127444.0
62	116438.5
63	105433.0
64	93612.5
65	81792.0
66	71262.0
67	60732.0
68	53347.5
69	45963.0
70	39642.5
71	33322.0
72	28865.5
73	24409.0
74	20349.5
75	14105.0
76	11920.0
77	9129.0
78	6338.0
79	4996.0
80	3654.0
81	2707.0
82	1760.0
83	1406.5
84	1053.0
85	721.5
86	390.0
87	307.5
88	225.0
89	153.5
90	82.0
91	55.5
92	29.0
93	22.5
94	16.0
95	12.5
96	9.0
97	6.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2555196.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.680065855170973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.00068893286453	14.959394801166702
2	12.09879369930377	5.246052883380668
3	4.965379036211004	3.2294923350281985
4	2.621167289849783	2.2730831784545327
5	1.5172748150032425	1.6447308954831323
6	1.0373706815515529	1.3494158815356758
7	0.7101121339172591	1.0776694479517517
8	0.5242616334784077	0.9092821391321109
9	0.41444670550195034	0.8086708681866841
>10	4.626059075007684	25.994386538583015
>50	2.250809359774183	33.59227592927317
>100	0.22566175811222136	6.896553099840838
>500	0.004893676010474297	0.7367723484788412
>1k	0.0030812034140023357	1.282219653504697
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC	4988	0.19521007390431105	Illumina PCR Primer Index 6 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGCT	3930	0.1538042482846717	Illumina PCR Primer Index 6 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC	3342	0.13079231495353	Illumina PCR Primer Index 6 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.827188207871333E-5	0.0	0.0	0.17274604374772032	0.0
2	7.827188207871333E-5	0.0	0.0	0.7242497248743345	0.0
3	7.827188207871333E-5	0.0	0.0	0.9985926715602247	0.0
4	7.827188207871333E-5	0.0	0.0	1.3258865464723646	0.0
5	7.827188207871333E-5	0.0	0.0	2.221982188450514	0.0
6	7.827188207871333E-5	0.0	0.0	2.7613145919138886	0.0
7	7.827188207871333E-5	0.0	0.0	3.346553454216428	0.0
8	7.827188207871333E-5	0.0	0.0	4.298574355939818	0.0
9	7.827188207871333E-5	0.0	0.0	4.597925168949858	0.0
10	7.827188207871333E-5	0.0	0.0	5.471791596417653	0.0
11	7.827188207871333E-5	0.0	0.0	6.795995297425325	0.0
12	7.827188207871333E-5	0.0	0.0	7.750012132141722	0.0
13	7.827188207871333E-5	0.0	0.0	8.132448547978315	0.0
14	7.827188207871333E-5	0.0	0.0	8.28006931757877	0.0
15	1.1740782311807E-4	0.0	0.0	8.588969300202411	0.0
16	1.1740782311807E-4	0.0	0.0	9.257998212270213	0.0
17	1.1740782311807E-4	0.0	0.0	10.030619960269192	0.0
18	1.1740782311807E-4	0.0	0.0	10.860067094657317	0.0
19	1.1740782311807E-4	0.0	0.0	11.382571043473769	0.0
20	1.1740782311807E-4	0.0	0.0	11.8928254427449	0.0
21	1.9567970519678333E-4	0.0	0.0	12.545456395517213	0.0
22	2.3481564623614E-4	0.0	0.0	13.223251758377831	0.0
23	3.130875283148533E-4	0.0	0.0	13.909109125092556	0.0
24	5.087672335116367E-4	0.0	0.0	14.462882690799454	0.0
25	0.0010175344670232734	0.0	0.0	14.968753864674177	0.0
26	0.0010958063491019866	0.0	0.0	15.42053916803251	0.0
27	0.0010958063491019866	0.0	0.0	15.900150125469827	0.0
28	0.0011349422901413434	0.0	0.0	16.343716881209897	0.0
29	0.0011740782311807	0.0	0.0	16.83123329873716	0.0
30	0.0011740782311807	0.0	0.0	17.422303416254564	0.0
31	0.0011740782311807	0.0	0.0	17.923047781853136	0.0
32	0.0011740782311807	0.0	0.0	18.413264579312116	0.0
33	0.0013306219953381267	0.0	0.0	18.925593183458332	0.0
34	0.00140889387741684	0.0	0.0	19.41076144452324	0.0
35	0.0014871657594955534	0.0	0.0	19.945201855356693	0.0
36	0.0015263017005349101	0.0	0.0	20.454125632632486	0.0
37	0.0015263017005349101	0.0	0.0	20.95099554006816	0.0
38	0.0015263017005349101	0.0	0.0	21.514944450445288	0.0
39	0.0016045735826136233	0.0	0.0	22.28009123370575	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGTAC	30	2.1665492E-6	44.999996	23
ATTACGG	170	0.0	42.35294	2
CTATCGT	40	3.4604454E-7	39.375	42
TATTACG	40	3.4604454E-7	39.375	1
ACACGAC	1425	0.0	39.0	26
ACGACCA	1375	0.0	38.945454	28
AACACGT	1300	0.0	38.942307	41
GCGAGAC	1445	0.0	38.927338	21
CACGACC	1450	0.0	38.637928	27
TTAACGG	240	0.0	38.437496	2
GACACGA	1465	0.0	37.935154	25
TGCGATG	150	0.0	37.500004	1
GTAATCG	60	1.5643309E-10	37.499996	1
CACGTTA	30	1.1403129E-4	37.499996	41
CGTAAGG	505	0.0	37.425743	2
GTCAAGC	1480	0.0	37.398647	16
GTTAACG	205	0.0	37.317074	1
CGACCAA	1445	0.0	37.214535	29
TCAAGCG	1500	0.0	36.899998	17
AGACACG	1515	0.0	36.38614	24
>>END_MODULE