FastQCFastQC Report
Sat 18 Jun 2016
SRR3552800_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552800_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353813
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC27330.20187426180720675Illumina PCR Primer Index 6 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC22620.16708363710497684Illumina PCR Primer Index 6 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGCT19950.14736156322919045Illumina PCR Primer Index 6 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16410.12121319561859727No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG253.891174E-545.0000041
GCGAGAC6500.040.15384321
CACGACC6350.039.33070827
GCGATAC750.039.09
AACGGGA5000.038.6999974
CGTAAGG2350.038.297872
ACACGAC6550.038.12977226
GACACGA6550.038.12977225
ACTACGG659.094947E-1238.076922
AACACGT6350.037.55905541
TCGCTAA301.14002934E-437.49999620
TACGTAG301.14002934E-437.4999961
AAACACG6550.036.75572640
CGACCAA6800.036.3970629
CGAGACA7050.036.3829822
ACGACCA6750.036.33333228
GTCAAGC7300.036.06164616
ACGTTAG504.8799848E-836.0000041
ACCGTAG250.002107240336.00000434
CGTCATT250.002107240336.00000421
Produced by FastQC (version 0.11.5)