##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552800_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1353813
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8116083979102	33.0	33.0	33.0	27.0	33.0
2	31.998284105707363	33.0	33.0	33.0	27.0	33.0
3	32.00615446889637	33.0	33.0	33.0	27.0	33.0
4	31.914336765860572	33.0	33.0	33.0	27.0	33.0
5	31.98387886657906	33.0	33.0	33.0	27.0	33.0
6	35.41782949343816	37.0	37.0	37.0	33.0	37.0
7	35.514554816654886	37.0	37.0	37.0	33.0	37.0
8	35.55678073707373	37.0	37.0	37.0	33.0	37.0
9	35.45867930061242	37.0	37.0	37.0	33.0	37.0
10	35.565996928674785	37.0	37.0	37.0	33.0	37.0
11	35.56339391038497	37.0	37.0	37.0	33.0	37.0
12	35.573316994296846	37.0	37.0	37.0	33.0	37.0
13	35.63340210206284	37.0	37.0	37.0	33.0	37.0
14	35.68080451288324	37.0	37.0	37.0	33.0	37.0
15	35.71336366248514	37.0	37.0	37.0	33.0	37.0
16	35.74581718450037	37.0	37.0	37.0	33.0	37.0
17	35.76400950500549	37.0	37.0	37.0	33.0	37.0
18	35.7444469804914	37.0	37.0	37.0	33.0	37.0
19	35.73944702850394	37.0	37.0	37.0	33.0	37.0
20	35.705954219674354	37.0	37.0	37.0	33.0	37.0
21	35.634659291940615	37.0	37.0	37.0	33.0	37.0
22	35.682154773222	37.0	37.0	37.0	33.0	37.0
23	35.70198025872111	37.0	37.0	37.0	33.0	37.0
24	35.70113302206435	37.0	37.0	37.0	33.0	37.0
25	35.694824913041906	37.0	37.0	37.0	33.0	37.0
26	35.66313885300259	37.0	37.0	37.0	33.0	37.0
27	35.65462733774901	37.0	37.0	37.0	33.0	37.0
28	35.63864433271065	37.0	37.0	37.0	33.0	37.0
29	35.624597340991706	37.0	37.0	37.0	33.0	37.0
30	35.6117971979882	37.0	37.0	37.0	33.0	37.0
31	35.59440262429154	37.0	37.0	37.0	33.0	37.0
32	35.579351062517496	37.0	37.0	37.0	33.0	37.0
33	35.5514661182896	37.0	37.0	37.0	33.0	37.0
34	35.52661039597049	37.0	37.0	37.0	33.0	37.0
35	35.50199695231173	37.0	37.0	37.0	33.0	37.0
36	35.486290942692975	37.0	37.0	37.0	33.0	37.0
37	35.4757392638422	37.0	37.0	37.0	33.0	37.0
38	35.4599224560556	37.0	37.0	37.0	33.0	37.0
39	35.45585690195027	37.0	37.0	37.0	33.0	37.0
40	35.43678188937468	37.0	37.0	37.0	33.0	37.0
41	35.430376277964534	37.0	37.0	37.0	33.0	37.0
42	35.41750300817026	37.0	37.0	37.0	33.0	37.0
43	35.40485502798392	37.0	37.0	37.0	33.0	37.0
44	35.377384468903756	37.0	37.0	37.0	33.0	37.0
45	35.33432239164493	37.0	37.0	37.0	33.0	37.0
46	35.34938355592685	37.0	37.0	37.0	33.0	37.0
47	35.33808879069709	37.0	37.0	37.0	33.0	37.0
48	35.33512678634346	37.0	37.0	37.0	33.0	37.0
49	35.335575888250446	37.0	37.0	37.0	33.0	37.0
50	35.319341740698306	37.0	37.0	37.0	33.0	37.0
51	34.97264688697774	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	963.0
15	3789.0
16	3646.0
17	2990.0
18	2893.0
19	2859.0
20	3000.0
21	3238.0
22	3650.0
23	4291.0
24	5178.0
25	6270.0
26	8023.0
27	10335.0
28	13309.0
29	16133.0
30	19042.0
31	23486.0
32	30773.0
33	42375.0
34	65636.0
35	143401.0
36	938533.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.41675992179127	18.970788432375816	23.223739172249047	12.388712473583869
2	30.68917199051863	22.989807307213034	25.93460101210433	20.386419690164004
3	33.10848691806032	24.694917244848437	24.60568778701342	17.590908050077818
4	29.21732912891219	26.847208587892123	24.216195294327946	19.71926698886774
5	26.32497989013254	30.068185192489654	23.42288041258283	20.183954504794976
6	27.28434429274944	35.793421986640695	21.831670991488487	15.090562729121379
7	83.16037739333275	6.232987864646003	6.484942898317566	4.1216918437036725
8	85.06211714616421	5.009332899004516	5.906576462184955	4.0219734926463255
9	80.11564374104843	6.626542956819	7.961660879309034	5.296152422823536
10	44.67071892499186	25.07384697886636	15.307357810864572	14.948076285277212
11	34.225923373464425	26.252517888364196	20.830424881427493	18.69113385674388
12	28.962345611986294	24.055094758286412	26.771865833759907	20.21069379596739
13	26.2602737601131	24.463053612278802	28.359899040709465	20.916773586898636
14	21.98966917883046	26.456238786302094	29.368974887964587	22.185117146902858
15	22.667310773349055	24.850034679826535	30.943490718437477	21.539163828386933
16	26.407192130670925	24.35365888789663	28.30656818925509	20.932580792177355
17	26.00972216990087	24.089442190317275	27.263883564421377	22.63695207536048
18	28.05047669065078	22.97894908676457	27.144221543152565	21.82635267943209
19	26.50144443878143	25.562836226273493	25.75592050009861	22.17979883484647
20	28.408871830895404	26.026194164186634	25.269664274164892	20.295269730753066
21	26.766621387148742	26.247273441753034	26.786121864688845	20.19998330640938
22	25.74801689745925	23.35935612968704	26.82416256898109	24.068464403872618
23	24.191967428293275	26.32431510112549	26.910511274452233	22.573206196129007
24	24.05893576143825	24.96563410160783	27.808050299413583	23.167379837540338
25	24.746992383733943	25.67090137264157	25.660117017638328	23.92198922598616
26	24.687752296661355	25.377507824197288	25.886736203596804	24.048003675544553
27	24.34767578683319	25.220986945759865	26.66926673033868	23.762070537068265
28	21.91912767863804	26.83908338891708	26.697261734079962	24.544527198364914
29	24.45581479864649	26.154720038882772	26.014154096614522	23.375311065856216
30	25.758062598010213	24.523623277365488	27.118811829994243	22.599502294630057
31	25.81154118035504	26.24454042027961	24.664780143195554	23.279138256169794
32	26.13832191004223	26.24010849356595	24.711463104579437	22.910106491812385
33	25.496061863787688	25.571183021584222	24.508850188319954	24.423904926308136
34	23.161766063703038	26.673033868045295	27.30303225039204	22.86216781785963
35	24.133392130227733	27.41331336011694	25.507732604133658	22.945561905521664
36	24.94532110417022	28.633718246168417	24.022446231495785	22.39851441816558
37	23.930336021296885	28.320233296622206	24.173796528767266	23.57563415331364
38	23.18377796638088	29.14279889467748	24.595715951907685	23.077707187033955
39	23.48551831013589	25.49886875070634	25.58344468549201	25.43216825366576
40	25.283994170539064	25.668685409284738	26.80170747363188	22.245612946544316
41	22.491067821035845	26.52330861056881	25.308886825580785	25.676736742814555
42	23.88564742693415	25.36857010532474	25.646156448490302	25.099626019250813
43	23.83468026972706	23.670772846766873	26.936733507508055	25.557813375998013
44	23.459813135196665	24.274401265167345	26.563048220101297	25.70273737953469
45	24.29574837883814	24.280458231676015	25.967471135230642	25.456322254255205
46	23.383583995721715	25.499164212487248	26.134333175999934	24.982918615791103
47	21.595153835869503	27.265877931442528	27.792095363244407	23.346872869443562
48	22.03450550408365	26.662175647596825	27.27584976654826	24.02746908177126
49	23.39252171459426	25.197423868732237	27.4521665843067	23.957887832366804
50	21.725157019470192	25.765301411642522	26.87897072934002	25.63057083954726
51	21.717253416830832	25.330086208361124	25.96370399752403	26.988956377284012
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	238.0
1	349.5
2	461.0
3	1840.0
4	3219.0
5	2520.0
6	1821.0
7	1745.5
8	1670.0
9	1860.5
10	2051.0
11	2135.5
12	2220.0
13	2395.0
14	2570.0
15	2612.5
16	2655.0
17	2603.5
18	2552.0
19	2757.0
20	2962.0
21	3416.5
22	3871.0
23	4323.5
24	4776.0
25	5954.0
26	8641.0
27	10150.0
28	11902.5
29	13655.0
30	15723.0
31	17791.0
32	20537.0
33	23283.0
34	26712.0
35	30141.0
36	33112.0
37	36083.0
38	39174.5
39	42266.0
40	45153.5
41	48041.0
42	55065.5
43	62090.0
44	67900.5
45	73711.0
46	83444.5
47	93178.0
48	104038.0
49	114898.0
50	117559.0
51	120220.0
52	112661.0
53	105102.0
54	98339.5
55	91577.0
56	86712.5
57	81848.0
58	78482.5
59	75117.0
60	71815.5
61	68514.0
62	62209.5
63	55905.0
64	49831.5
65	43758.0
66	38207.5
67	32657.0
68	28681.0
69	24705.0
70	21263.0
71	17821.0
72	15260.5
73	12700.0
74	10477.5
75	7091.0
76	5927.0
77	4469.0
78	3011.0
79	2391.5
80	1772.0
81	1302.5
82	833.0
83	625.0
84	417.0
85	252.0
86	87.0
87	86.0
88	85.0
89	50.0
90	15.0
91	8.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1353813.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.853806002916564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.60194282428816	18.511827392110693
2	10.441941219387067	5.399278451597825
3	3.871255839744081	3.0026009240520395
4	1.8861990689218744	1.9506169924315193
5	1.1515993203990469	1.4886612711343756
6	0.7896698377399249	1.2249582474769571
7	0.615910334027749	1.1146538403801474
8	0.4801194818011478	0.9930332752566164
9	0.411455516841287	0.9573922000119963
>10	8.127373393664431	52.741921466201994
>50	0.5640110753815919	8.755968232171355
>100	0.05307150119802891	2.440632672200892
>500	0.0043030946886311065	0.7783146215431767
>1k	0.001147491916968295	0.6401404134304515
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC	2733	0.20187426180720675	Illumina PCR Primer Index 6 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC	2262	0.16708363710497684	Illumina PCR Primer Index 6 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGCT	1995	0.14736156322919045	Illumina PCR Primer Index 6 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1641	0.12121319561859727	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16811775333816414	0.0
2	0.0	0.0	0.0	0.7130231427826442	0.0
3	0.0	0.0	0.0	0.9418582920979486	0.0
4	0.0	0.0	0.0	1.2064443169034424	0.0
5	0.0	0.0	0.0	1.8946486700895915	0.0
6	0.0	0.0	0.0	2.2961073649019474	0.0
7	0.0	0.0	0.0	2.7441012902077317	0.0
8	0.0	0.0	0.0	3.5481266615108584	0.0
9	0.0	0.0	0.0	3.837679206803303	0.0
10	0.0	0.0	0.0	4.668443869278844	0.0
11	0.0	0.0	0.0	5.897269416086269	0.0
12	0.0	0.0	0.0	6.798944905980368	0.0
13	0.0	0.0	0.0	7.161845838383883	0.0
14	0.0	0.0	0.0	7.310093786955806	0.0
15	0.0	0.0	0.0	7.591890460499346	0.0
16	0.0	0.0	0.0	8.186728890917726	0.0
17	0.0	0.0	0.0	8.88424029020256	0.0
18	0.0	0.0	0.0	9.666918547834893	0.0
19	0.0	0.0	0.0	10.16262955075775	0.0
20	0.0	0.0	0.0	10.641573097613925	0.0
21	7.386544522766439E-5	0.0	0.0	11.250002769954197	0.0
22	7.386544522766439E-5	0.0	0.0	11.887092235042802	0.0
23	1.4773089045532877E-4	0.0	0.0	12.53984117451967	0.0
24	1.4773089045532877E-4	0.0	0.0	13.062439199505397	0.0
25	7.386544522766438E-4	0.0	0.0	13.543894171499314	0.0
26	7.386544522766438E-4	0.0	0.0	14.002229259136971	0.0
27	7.386544522766438E-4	0.0	0.0	14.472678279791966	0.0
28	8.125198975043082E-4	0.0	0.0	14.914245911362944	0.0
29	9.602507879596369E-4	0.0	0.0	15.379893678078139	0.0
30	9.602507879596369E-4	0.0	0.0	15.91778185022599	0.0
31	0.0010341162331873014	0.0	0.0	16.38468532951006	0.0
32	0.0010341162331873014	0.0	0.0	16.841690839133616	0.0
33	0.0011079816784149658	0.0	0.0	17.310514819993603	0.0
34	0.0011079816784149658	0.0	0.0	17.768185118624213	0.0
35	0.0011818471236426302	0.0	0.0	18.282879540970576	0.0
36	0.0012557125688702946	0.0	0.0	18.75909006635333	0.0
37	0.0012557125688702946	0.0	0.0	19.239289325778376	0.0
38	0.0012557125688702946	0.0	0.0	19.78973462361493	0.0
39	0.001551174349780952	0.0	0.0	20.505860115097136	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	25	3.891174E-5	45.000004	1
GCGAGAC	650	0.0	40.153843	21
CACGACC	635	0.0	39.330708	27
GCGATAC	75	0.0	39.0	9
AACGGGA	500	0.0	38.699997	4
CGTAAGG	235	0.0	38.29787	2
ACACGAC	655	0.0	38.129772	26
GACACGA	655	0.0	38.129772	25
ACTACGG	65	9.094947E-12	38.07692	2
AACACGT	635	0.0	37.559055	41
TCGCTAA	30	1.14002934E-4	37.499996	20
TACGTAG	30	1.14002934E-4	37.499996	1
AAACACG	655	0.0	36.755726	40
CGACCAA	680	0.0	36.39706	29
CGAGACA	705	0.0	36.38298	22
ACGACCA	675	0.0	36.333332	28
GTCAAGC	730	0.0	36.061646	16
ACGTTAG	50	4.8799848E-8	36.000004	1
ACCGTAG	25	0.0021072403	36.000004	34
CGTCATT	25	0.0021072403	36.000004	21
>>END_MODULE