FastQCFastQC Report
Sat 18 Jun 2016
SRR3552798_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552798_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697472
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC59180.8484928427234355Illumina PCR Primer Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC35680.5115617544503579Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT35360.5069737566525968Illumina PCR Primer Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC17020.2440241328684162Illumina PCR Primer Index 10 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC10520.15083042760139476Illumina PCR Primer Index 10 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG8760.12559643971370893Illumina PCR Primer Index 10 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCC8000.11469994494402642No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG7810.1119758212516058Illumina PCR Primer Index 10 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG7580.10867819783446503Illumina PCR Primer Index 10 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAG551.8189894E-1245.0000041
TCGTTGA351.2110104E-745.024
CAACGCG207.0313766E-445.01
CGGCATC207.0313766E-445.022
TTCACCG207.0313766E-445.023
TCGACGT207.0313766E-445.026
CGATAGA207.0313766E-445.010
TATTGCG253.8892238E-544.9999961
TATAGCG253.8892238E-544.9999961
ACGAGAC253.8892238E-544.99999612
CGTAAGG1500.043.5000042
CGAAAGG1050.042.8571432
GCGTAAG900.042.51
AATGGGT750.042.0000044
TGTAGCG700.041.7857131
CACGACC1150.041.08695627
CGAGGGT556.002665E-1140.9090924
CGAATAT501.0804797E-940.49999614
CGTCAGG501.0804797E-940.4999962
GCGAAAG950.040.263161
Produced by FastQC (version 0.11.5)