##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552798_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	697472
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.684216714075976	33.0	33.0	33.0	27.0	33.0
2	31.87496702376583	33.0	33.0	33.0	27.0	33.0
3	31.928896070379885	33.0	33.0	33.0	27.0	33.0
4	31.839752706918702	33.0	33.0	33.0	27.0	33.0
5	31.898935011011194	33.0	33.0	33.0	27.0	33.0
6	35.35537483942008	37.0	37.0	37.0	33.0	37.0
7	35.68502248118921	37.0	37.0	37.0	33.0	37.0
8	35.74864940814828	37.0	37.0	37.0	33.0	37.0
9	35.6163616603964	37.0	37.0	37.0	33.0	37.0
10	35.592151369517346	37.0	37.0	37.0	33.0	37.0
11	35.5938589649477	37.0	37.0	37.0	33.0	37.0
12	35.61243892227932	37.0	37.0	37.0	33.0	37.0
13	35.66081935905671	37.0	37.0	37.0	33.0	37.0
14	35.70862486236007	37.0	37.0	37.0	33.0	37.0
15	35.74471089878877	37.0	37.0	37.0	33.0	37.0
16	35.76074308359332	37.0	37.0	37.0	33.0	37.0
17	35.77196360570747	37.0	37.0	37.0	33.0	37.0
18	35.75703254037438	37.0	37.0	37.0	33.0	37.0
19	35.745019154890805	37.0	37.0	37.0	33.0	37.0
20	35.72443768351991	37.0	37.0	37.0	33.0	37.0
21	35.637563945219306	37.0	37.0	37.0	33.0	37.0
22	35.679677463754814	37.0	37.0	37.0	33.0	37.0
23	35.698861029546705	37.0	37.0	37.0	33.0	37.0
24	35.686859114057626	37.0	37.0	37.0	33.0	37.0
25	35.68054201458983	37.0	37.0	37.0	33.0	37.0
26	35.62896575059644	37.0	37.0	37.0	33.0	37.0
27	35.61486625986419	37.0	37.0	37.0	33.0	37.0
28	35.607164158561204	37.0	37.0	37.0	33.0	37.0
29	35.608597907873005	37.0	37.0	37.0	33.0	37.0
30	35.578309953661226	37.0	37.0	37.0	33.0	37.0
31	35.53827537162782	37.0	37.0	37.0	33.0	37.0
32	35.51601497981281	37.0	37.0	37.0	33.0	37.0
33	35.5001577124243	37.0	37.0	37.0	33.0	37.0
34	35.48042932189392	37.0	37.0	37.0	33.0	37.0
35	35.473838376307576	37.0	37.0	37.0	33.0	37.0
36	35.45813738759406	37.0	37.0	37.0	33.0	37.0
37	35.44851406221325	37.0	37.0	37.0	33.0	37.0
38	35.43502391493852	37.0	37.0	37.0	33.0	37.0
39	35.41978029225546	37.0	37.0	37.0	33.0	37.0
40	35.402053415764364	37.0	37.0	37.0	33.0	37.0
41	35.39354698109745	37.0	37.0	37.0	33.0	37.0
42	35.38555239493485	37.0	37.0	37.0	33.0	37.0
43	35.38367991833364	37.0	37.0	37.0	33.0	37.0
44	35.36118439163149	37.0	37.0	37.0	33.0	37.0
45	35.306370721692055	37.0	37.0	37.0	33.0	37.0
46	35.32927056570013	37.0	37.0	37.0	33.0	37.0
47	35.31396529179666	37.0	37.0	37.0	33.0	37.0
48	35.29683772251789	37.0	37.0	37.0	33.0	37.0
49	35.3028408308864	37.0	37.0	37.0	33.0	37.0
50	35.29753309093412	37.0	37.0	37.0	33.0	37.0
51	34.88876972839053	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	440.0
15	1742.0
16	1400.0
17	1016.0
18	947.0
19	965.0
20	1082.0
21	1208.0
22	1451.0
23	1826.0
24	2383.0
25	3137.0
26	4355.0
27	5916.0
28	7700.0
29	9623.0
30	11581.0
31	14495.0
32	18389.0
33	25608.0
34	37850.0
35	79451.0
36	464907.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.05502156358965	19.374684575151406	24.939639153973207	13.630654707285741
2	28.69534547623417	23.881102037071024	27.233207928060192	20.190344558634614
3	31.305199348504313	24.417324279684347	26.707452055423015	17.570024316388327
4	28.42178610754267	26.558628876858137	25.619666452560104	19.39991856303909
5	25.192409157643603	29.412650256927876	25.25592425215636	20.13901633327216
6	25.966777160946965	35.09789640300973	22.906009130115617	16.02931730592769
7	81.15322192145348	5.9879106258029	7.698947054505415	5.159920398238209
8	82.4878991558084	4.355013534593503	7.265668012479354	5.891419297118737
9	76.80021563589649	7.383522205909341	9.825770783630023	5.99049137456414
10	39.103935355111034	31.93662254542118	15.810383786015784	13.149058313452008
11	26.150153697926225	25.6986660396403	27.98578294182419	20.165397320609284
12	25.13620618462103	22.694531106625067	31.8546120847862	20.3146506239677
13	23.318355432189392	25.028531611304828	32.32774935768031	19.325363598825472
14	19.83334097999633	28.159553358414385	29.64964901816847	22.35745664342081
15	18.233993622683062	26.81555675353276	33.9455920811158	21.00485754266838
16	21.208163195081667	25.982405028445587	31.150354422829878	21.659077353642868
17	20.97646930629473	25.90197169205359	28.65161612222426	24.469942879427418
18	21.373904615525785	25.096061203890624	31.595103459350337	21.934930721233254
19	21.4506102037071	26.880505597357313	29.03155968985135	22.637324509084237
20	23.786761332354562	27.019579280601945	28.867682143512567	20.325977243530925
21	22.937121490181685	27.78491466324096	29.17393099651312	20.104032850064232
22	20.592224490732246	25.888207698660302	27.881692741787482	25.637875068819966
23	20.12955358781428	27.720539319141125	28.15926660855203	23.990640484492566
24	21.254760047715177	25.34840108276748	29.617963158377687	23.77887571113966
25	19.631612451826022	27.82018489631125	28.07496214901817	24.473240502844558
26	19.464007157276562	27.710789823820885	29.362325656083687	23.462877362818865
27	21.14794572398605	25.90928381354377	29.001450954303543	23.941319508166636
28	18.697524775188107	26.57267962011378	30.201929253073956	24.52786635162415
29	20.218159295283538	24.00340085336759	31.788946366305744	23.989493485043127
30	21.713129702697742	24.370870801982015	30.00120434942191	23.914795145898328
31	22.168058359331987	26.010649889888054	26.945024316388327	24.87626743439163
32	21.01059253991558	25.5263293723619	29.85897641769132	23.6041016700312
33	21.296195402826207	24.588083822719764	27.861907001284642	26.253813773169387
34	19.65698981464489	23.641522527069185	30.767113231785647	25.934374426500273
35	18.776094237474766	22.724353092310515	32.2911887502294	26.208363919985317
36	20.852019866030464	24.377035924022756	29.364189530189027	25.406754679757753
37	21.23597793173059	24.57159570563406	30.427027895026608	23.765398467608737
38	20.26317902367407	25.751141264452194	30.615279179665993	23.370400532207743
39	21.198413699761424	23.00321733345568	31.072788585061478	24.725580381721418
40	21.340785006423197	22.388712378418056	32.07684322811525	24.193659387043496
41	18.249764865112862	23.22745572582125	29.919337263718116	28.603442145347767
42	20.637100844191593	22.431438107909706	29.71416773719949	27.21729331069921
43	22.08590452376583	22.057659662323363	29.835462928977798	26.020972884933013
44	20.96714993576803	22.91059712791338	29.971812488530002	26.150440447788586
45	21.107083868599744	21.323866764543954	30.202216002936318	27.366833363919984
46	21.498497430721233	22.363908515323914	31.204263396953568	24.933330657001285
47	18.513144613690585	23.139280143145534	32.819668746559	25.527906496604878
48	19.3546120847862	21.970057579372362	31.754106258028997	26.921224077812443
49	21.49821068085887	20.590790741420445	32.83558336391999	25.075415213800696
50	20.10489309965131	20.831230501009358	31.399540053220775	27.664336346118557
51	19.021121994861442	20.143460956138743	29.057223802532572	31.77819324646724
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	254.0
1	293.5
2	333.0
3	797.0
4	1261.0
5	1001.0
6	741.0
7	787.5
8	834.0
9	855.0
10	876.0
11	973.5
12	1071.0
13	1153.5
14	1236.0
15	1288.5
16	1341.0
17	1454.0
18	1567.0
19	1756.0
20	1945.0
21	2218.5
22	2492.0
23	2620.5
24	2749.0
25	3825.5
26	5102.5
27	5303.0
28	6383.0
29	7463.0
30	8795.5
31	10128.0
32	12092.5
33	14057.0
34	15796.0
35	17535.0
36	18773.5
37	20012.0
38	22954.0
39	25896.0
40	29093.0
41	32290.0
42	35827.5
43	39365.0
44	43566.0
45	47767.0
46	56905.0
47	66043.0
48	75788.5
49	85534.0
50	84039.0
51	82544.0
52	69316.5
53	56089.0
54	47697.0
55	39305.0
56	34429.0
57	29553.0
58	26617.0
59	23681.0
60	22191.0
61	20701.0
62	19253.5
63	17806.0
64	14854.5
65	11903.0
66	9754.0
67	7605.0
68	6214.5
69	4824.0
70	4183.0
71	3542.0
72	3124.0
73	2706.0
74	2253.0
75	1496.0
76	1192.0
77	906.0
78	620.0
79	487.0
80	354.0
81	263.5
82	173.0
83	119.0
84	65.0
85	35.5
86	6.0
87	6.5
88	7.0
89	3.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	697472.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.89897526796238
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.54229977658953	21.487858008740655
2	12.36498461304999	7.64130707494734
3	4.795460198431203	4.4452441820947115
4	2.343567898127962	2.896553860921859
5	1.3468550260272745	2.0808220069373795
6	0.9240801537730573	1.7131877890229141
7	0.6756317187602721	1.4613429437757193
8	0.5511363422206773	1.3623638566041567
9	0.4532252456715797	1.260377609114008
>10	6.787184320116397	46.67749148317869
>50	0.15610582248535432	3.016559956489139
>100	0.0538936768017318	2.971339550324963
>500	0.0032522046345872632	0.7207989120775746
>1k	0.0018584026483355791	1.415183364919484
>5k	4.646006620838948E-4	0.8495694008514412
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	5918	0.8484928427234355	Illumina PCR Primer Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	3568	0.5115617544503579	Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT	3536	0.5069737566525968	Illumina PCR Primer Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	1702	0.2440241328684162	Illumina PCR Primer Index 10 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	1052	0.15083042760139476	Illumina PCR Primer Index 10 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG	876	0.12559643971370893	Illumina PCR Primer Index 10 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCC	800	0.11469994494402642	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG	781	0.1119758212516058	Illumina PCR Primer Index 10 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG	758	0.10867819783446503	Illumina PCR Primer Index 10 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8674986236006605E-4	0.0	0.0	0.5205943751147	0.0
2	2.8674986236006605E-4	0.0	0.0	2.3198063864929344	0.0
3	2.8674986236006605E-4	0.0	0.0	3.0609114057625253	0.0
4	2.8674986236006605E-4	0.0	0.0	3.7911199302624334	0.0
5	2.8674986236006605E-4	0.0	0.0	5.575564323729124	0.0
6	2.8674986236006605E-4	0.0	0.0	6.456890025692788	0.0
7	2.8674986236006605E-4	0.0	0.0	7.50739814644889	0.0
8	2.8674986236006605E-4	0.0	0.0	9.298581161681042	0.0
9	2.8674986236006605E-4	0.0	0.0	9.930721233253808	0.0
10	2.8674986236006605E-4	0.0	0.0	11.50354422829877	0.0
11	2.8674986236006605E-4	0.0	0.0	13.946366305744172	0.0
12	2.8674986236006605E-4	0.0	0.0	15.75676156175445	0.0
13	2.8674986236006605E-4	0.0	0.0	16.427612864745825	0.0
14	2.8674986236006605E-4	0.0	0.0	16.66303450174344	0.0
15	2.8674986236006605E-4	0.0	0.0	17.112084786199304	0.0
16	2.8674986236006605E-4	0.0	0.0	18.111551431455315	0.0
17	2.8674986236006605E-4	0.0	0.0	19.44995641402092	0.0
18	2.8674986236006605E-4	0.0	0.0	20.982060928610753	0.0
19	2.8674986236006605E-4	0.0	0.0	21.87184575151404	0.0
20	2.8674986236006605E-4	0.0	0.0	22.638471508533677	0.0
21	2.8674986236006605E-4	0.0	0.0	23.6112704165902	0.0
22	7.168746559001652E-4	0.0	0.0	24.669377408698843	0.0
23	8.602495870801983E-4	0.0	0.0	25.677446549825657	0.0
24	0.0010036245182602312	0.0	0.0	26.48464741236924	0.0
25	0.003727748210680859	0.0	0.0	27.16868920902918	0.0
26	0.0038711231418608917	0.0	0.0	27.77588204257662	0.0
27	0.0038711231418608917	0.0	0.0	28.38393512571114	0.0
28	0.0038711231418608917	0.0	0.0	29.009910075243162	0.0
29	0.0038711231418608917	0.0	0.0	29.667570884565976	0.0
30	0.0038711231418608917	0.0	0.0	30.43333639199853	0.0
31	0.0038711231418608917	0.0	0.0	31.091714075977244	0.0
32	0.0038711231418608917	0.0	0.0	31.699910534042942	0.0
33	0.0038711231418608917	0.0	0.0	32.29104537529822	0.0
34	0.004014498073040925	0.0	0.0	32.876588594237475	0.0
35	0.005018122591301156	0.0	0.0	33.51561066250688	0.0
36	0.0051614975224811895	0.0	0.0	34.104594879794455	0.0
37	0.00616512204074142	0.0	0.0	34.72139383373096	0.0
38	0.00616512204074142	0.0	0.0	35.30994792622499	0.0
39	0.00616512204074142	0.0	0.0	35.89993576803083	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGAG	55	1.8189894E-12	45.000004	1
TCGTTGA	35	1.2110104E-7	45.0	24
CAACGCG	20	7.0313766E-4	45.0	1
CGGCATC	20	7.0313766E-4	45.0	22
TTCACCG	20	7.0313766E-4	45.0	23
TCGACGT	20	7.0313766E-4	45.0	26
CGATAGA	20	7.0313766E-4	45.0	10
TATTGCG	25	3.8892238E-5	44.999996	1
TATAGCG	25	3.8892238E-5	44.999996	1
ACGAGAC	25	3.8892238E-5	44.999996	12
CGTAAGG	150	0.0	43.500004	2
CGAAAGG	105	0.0	42.857143	2
GCGTAAG	90	0.0	42.5	1
AATGGGT	75	0.0	42.000004	4
TGTAGCG	70	0.0	41.785713	1
CACGACC	115	0.0	41.086956	27
CGAGGGT	55	6.002665E-11	40.909092	4
CGAATAT	50	1.0804797E-9	40.499996	14
CGTCAGG	50	1.0804797E-9	40.499996	2
GCGAAAG	95	0.0	40.26316	1
>>END_MODULE