##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552797_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	924189
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.646110265324516	31.0	31.0	34.0	30.0	34.0
2	31.810186011735695	31.0	31.0	34.0	30.0	34.0
3	31.856734931924098	33.0	31.0	34.0	30.0	34.0
4	35.492900261742996	37.0	35.0	37.0	33.0	37.0
5	35.36459858319024	37.0	35.0	37.0	33.0	37.0
6	35.33796009257846	37.0	35.0	37.0	33.0	37.0
7	35.76225209345707	37.0	35.0	37.0	35.0	37.0
8	35.73876663756007	37.0	35.0	37.0	35.0	37.0
9	37.343580155141424	39.0	37.0	39.0	35.0	39.0
10	36.749401908051276	39.0	37.0	39.0	32.0	39.0
11	36.615015976169374	39.0	35.0	39.0	32.0	39.0
12	36.46991578562394	38.0	35.0	39.0	32.0	39.0
13	36.3782711112121	38.0	35.0	39.0	32.0	39.0
14	37.388517933020196	39.0	36.0	41.0	32.0	41.0
15	37.50282031056418	39.0	36.0	41.0	32.0	41.0
16	37.54417765197378	39.0	36.0	41.0	33.0	41.0
17	37.53037636241072	39.0	36.0	41.0	32.0	41.0
18	37.5209183402962	39.0	36.0	41.0	32.0	41.0
19	37.4969535452164	39.0	36.0	41.0	32.0	41.0
20	37.420423744493824	39.0	36.0	41.0	32.0	41.0
21	37.35989932795132	39.0	35.0	41.0	32.0	41.0
22	37.310527392124335	39.0	35.0	41.0	32.0	41.0
23	37.28631156614069	39.0	35.0	40.0	32.0	41.0
24	37.24581119229941	39.0	35.0	41.0	32.0	41.0
25	37.230293803540185	39.0	35.0	41.0	32.0	41.0
26	37.14458406235088	39.0	35.0	40.0	32.0	41.0
27	37.085815780105584	39.0	35.0	40.0	31.0	41.0
28	37.017770174715345	39.0	35.0	40.0	31.0	41.0
29	37.01349940326059	39.0	35.0	40.0	31.0	41.0
30	36.899274931859175	39.0	35.0	40.0	31.0	41.0
31	36.75998415908434	39.0	35.0	40.0	31.0	41.0
32	36.66810143812575	39.0	35.0	40.0	30.0	41.0
33	36.58751402581074	39.0	35.0	40.0	30.0	41.0
34	36.44193882420154	39.0	35.0	40.0	30.0	41.0
35	36.43910823435466	39.0	35.0	40.0	30.0	41.0
36	36.3768882771814	38.0	35.0	40.0	30.0	41.0
37	36.28059087481024	38.0	35.0	40.0	30.0	41.0
38	36.236182209483125	38.0	35.0	40.0	30.0	41.0
39	36.1887016616731	38.0	35.0	40.0	30.0	41.0
40	36.03109969930393	38.0	35.0	40.0	30.0	41.0
41	35.9365010836528	38.0	35.0	40.0	29.0	41.0
42	35.92658319889114	38.0	35.0	40.0	29.0	41.0
43	35.89900983456847	38.0	35.0	40.0	29.0	41.0
44	35.75322255512671	38.0	34.0	40.0	29.0	41.0
45	35.68269585550142	38.0	34.0	40.0	29.0	41.0
46	35.53193123917294	38.0	34.0	40.0	28.0	41.0
47	35.414532092461606	38.0	34.0	40.0	28.0	41.0
48	35.37548813067457	38.0	34.0	40.0	27.0	41.0
49	35.34398483427091	38.0	34.0	40.0	27.0	41.0
50	35.242150685628154	37.0	34.0	40.0	27.0	41.0
51	33.621578486651536	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	5.0
10	4.0
11	3.0
12	8.0
13	10.0
14	17.0
15	49.0
16	91.0
17	210.0
18	356.0
19	596.0
20	971.0
21	1478.0
22	2316.0
23	3348.0
24	4595.0
25	6130.0
26	7853.0
27	9827.0
28	12496.0
29	15694.0
30	20044.0
31	25925.0
32	33619.0
33	44650.0
34	69875.0
35	81683.0
36	84523.0
37	117645.0
38	182914.0
39	197227.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.79178825976072	19.757214162903907	26.75935333573544	10.691644241599931
2	30.37019484109852	24.7141006871971	26.015566080098335	18.90013839160605
3	31.465425362128308	24.274688402480447	26.852624300873522	17.407261934517724
4	29.203983167945086	25.33615959506118	26.361274587773714	19.09858264922002
5	24.542057955677897	30.470174390736094	25.53546947648154	19.452298177104467
6	26.2513403643627	35.533532643214755	23.520297255215112	14.694829737207431
7	80.7031894991176	5.856918876982955	8.293433486007732	5.146458137891708
8	80.47791090350567	5.159009683084304	8.320376026981494	6.042703386428534
9	75.14902254841813	7.167906131754435	10.836636229169574	6.846435090657864
10	39.498089676462286	24.47053578867526	18.719547624998782	17.311826909863676
11	29.381219642302604	26.694756159183893	23.443364939422565	20.480659259090945
12	26.538294656179634	23.707704809297667	29.381003236351006	20.372997298171693
13	23.717118468192112	25.76507619112541	30.940424523555247	19.57738081712723
14	19.022407754258058	29.398423915454526	29.309156460420976	22.270011869866448
15	19.346042854870596	25.898815069212034	33.99164023808983	20.763501837827544
16	20.49115494774337	26.364412474071862	31.116795374106378	22.02763720407839
17	20.42244605811149	25.333995535545217	28.006933646689152	26.23662475965414
18	21.084864675948317	25.101575543530597	31.182258174464316	22.63130160605677
19	22.366096112375285	26.918087101231457	27.72603872151692	22.98977806487634
20	24.14711709401432	27.39710167509027	27.510606596702626	20.94517463419279
21	23.268617133508407	26.804473976643305	29.17671601804393	20.75019287180436
22	21.059653382587328	25.826968293282004	26.528880997285185	26.584497326845486
23	20.730716336160675	26.761517395251406	27.643696256934458	24.86407001165346
24	21.91315845568385	25.105470850659334	29.045682214352258	23.935688479304556
25	20.291087645492425	27.276347154099433	26.725161195383194	25.707404005024948
26	19.358486197087394	28.092305794593962	29.360661076900936	23.188546931417708
27	22.96640622210392	27.032674052601795	26.99404559024182	23.006874135052463
28	18.041439575671212	26.289211405892082	29.171414072229812	26.49793494620689
29	20.82582675188733	24.282803625665313	28.729404916094005	26.161964706353352
30	24.81667710825383	25.14399111004351	26.625506254673013	23.413825527029644
31	22.443353037095225	27.99027038841622	24.50862323615624	25.05775333833231
32	22.216992411725307	26.49501346586034	26.304576228455435	24.98341789395892
33	23.853021405794703	25.45680591307622	25.634366996361134	25.05580568476794
34	19.20537898633288	25.721037579975526	29.10843994031524	25.965143493376353
35	20.83069588579825	23.91177562165315	29.2154526833797	26.042075809168903
36	24.660756620128566	25.292986607717687	27.928702895187023	22.117553876966724
37	21.888812786129243	25.890483440075567	28.345825366889237	23.874878406905946
38	21.03433388625054	26.401742500722253	26.878484812089305	25.685438800937906
39	23.72339424078841	24.071050402028156	28.29745863670743	23.908096720476006
40	23.464789128630613	24.334200039169477	29.918231011189274	22.28277982101064
41	19.742498558195347	25.504631628379045	29.656812621660723	25.096057191764885
42	21.248900387258452	23.206075813497023	31.53197019224423	24.013053607000302
43	23.94661697986018	21.292938998408335	29.8302619918653	24.930182029866184
44	21.705516945127023	22.757574478813318	27.509199958017245	28.027708618042414
45	22.931132051993693	22.065941057510962	27.3708083519713	27.632118538524047
46	23.51542812130419	24.072132431786137	28.952519452189975	23.459919994719694
47	19.712634536875033	24.29405673514833	32.06151555580082	23.931793172175823
48	20.07251763437998	22.509356852332154	31.696005903554358	25.722119609733507
49	22.080548459243726	21.51962423270565	32.1301162424569	24.26971106559373
50	21.35277524402476	21.724019653988524	30.197719297676123	26.72548580431059
51	20.66103361974661	21.218495351059143	28.08960072019901	30.03087030899524
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	360.0
1	508.0
2	656.0
3	1093.5
4	1531.0
5	1209.5
6	888.0
7	902.0
8	916.0
9	1024.0
10	1132.0
11	1192.5
12	1253.0
13	1327.5
14	1402.0
15	1498.5
16	1595.0
17	1648.0
18	1701.0
19	1988.5
20	2276.0
21	2482.5
22	2689.0
23	2784.5
24	2880.0
25	4052.0
26	6023.5
27	6823.0
28	8469.0
29	10115.0
30	11949.5
31	13784.0
32	15827.5
33	17871.0
34	18746.5
35	19622.0
36	22291.5
37	24961.0
38	26495.0
39	28029.0
40	32907.5
41	37786.0
42	43418.5
43	49051.0
44	54843.0
45	60635.0
46	74446.5
47	88258.0
48	101301.5
49	114345.0
50	111687.5
51	109030.0
52	93315.0
53	77600.0
54	66690.5
55	55781.0
56	49256.0
57	42731.0
58	39265.5
59	35800.0
60	32690.0
61	29580.0
62	26880.5
63	24181.0
64	20881.5
65	17582.0
66	14897.0
67	12212.0
68	10090.0
69	7968.0
70	6705.0
71	5442.0
72	4854.5
73	4267.0
74	3515.0
75	2143.5
76	1524.0
77	1229.0
78	934.0
79	730.0
80	526.0
81	373.5
82	221.0
83	174.5
84	128.0
85	101.0
86	74.0
87	51.5
88	29.0
89	21.5
90	14.0
91	13.0
92	12.0
93	9.5
94	7.0
95	3.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	924189.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.271774894859053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.33684410926185	15.858411725605013
2	13.300134665634438	6.456357493511811
3	6.221981339124738	4.530555913897479
4	3.34705630535532	3.2495598881601175
5	1.9496563750979106	2.3660810329351682
6	1.3035933118139191	1.898431405127475
7	0.8597902872510504	1.4608045416260589
8	0.6527908893317369	1.2675514815419813
9	0.4965996052807797	1.084801844902585
>10	4.950523652864598	30.559639271350797
>50	1.457211206335716	23.147558579903592
>100	0.1153253039104135	4.940933402299449
>500	0.005363967623740163	0.9689615119548621
>1k	0.0026819838118700813	1.6449887408195745
>5k	4.469973019783469E-4	0.5653631663639891
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	5211	0.5638457068846308	Illumina Paired End PCR Primer 2 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	4357	0.47144036555293345	Illumina Paired End PCR Primer 2 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT	4157	0.4497997703932854	TruSeq Adapter, Index 1 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	2805	0.30350934711406435	Illumina Paired End PCR Primer 2 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	1668	0.180482563631465	TruSeq Adapter, Index 1 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTG	1151	0.1245416251437747	Illumina Paired End PCR Primer 2 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCC	1024	0.11079984721739818	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.082029757982404E-4	0.0	0.0	0.470358335794951	0.0
2	1.082029757982404E-4	0.0	0.0	2.074900263907058	0.0
3	1.082029757982404E-4	0.0	0.0	2.8290750052207936	0.0
4	1.082029757982404E-4	0.0	0.0	3.6605066712544727	0.0
5	1.082029757982404E-4	0.0	0.0	5.695263631140383	0.0
6	1.082029757982404E-4	0.0	0.0	6.736933679150044	0.0
7	1.082029757982404E-4	0.0	0.0	7.86743837029006	0.0
8	1.082029757982404E-4	0.0	0.0	9.70169521602183	0.0
9	1.082029757982404E-4	0.0	0.0	10.289237374606277	0.0
10	1.082029757982404E-4	0.0	0.0	11.932191359126758	0.0
11	1.082029757982404E-4	0.0	0.0	14.431247288162918	0.0
12	1.082029757982404E-4	0.0	0.0	16.211186240043975	0.0
13	1.082029757982404E-4	0.0	0.0	16.897301309580616	0.0
14	1.082029757982404E-4	0.0	0.0	17.143246673570015	0.0
15	1.082029757982404E-4	0.0	0.0	17.66510962584493	0.0
16	1.082029757982404E-4	0.0	0.0	18.877199360736817	0.0
17	1.082029757982404E-4	0.0	0.0	20.24899668790691	0.0
18	1.082029757982404E-4	0.0	0.0	21.87799248854942	0.0
19	1.082029757982404E-4	0.0	0.0	22.7338780271135	0.0
20	1.082029757982404E-4	0.0	0.0	23.538475355149217	0.0
21	0.001082029757982404	0.0	0.0	24.61325551375314	0.0
22	0.0020558565401665677	0.0	0.0	25.610454138709724	0.0
23	0.002488668443359529	0.0	0.0	26.605705110101937	0.0
24	0.004869133910920818	0.0	0.0	27.434215295789066	0.0
25	0.006383975572096184	0.0	0.0	28.128986603389567	0.0
26	0.0066003815236926645	0.0	0.0	28.768141581429774	0.0
27	0.0066003815236926645	0.0	0.0	29.35265405669187	0.0
28	0.0066003815236926645	0.0	0.0	29.98088053417645	0.0
29	0.0066003815236926645	0.0	0.0	30.643407354989076	0.0
30	0.0066003815236926645	0.0	0.0	31.336988429855797	0.0
31	0.0066003815236926645	0.0	0.0	31.988370344161204	0.0
32	0.0066003815236926645	0.0	0.0	32.594523414582945	0.0
33	0.0066003815236926645	0.0	0.0	33.20489640106082	0.0
34	0.0066003815236926645	0.0	0.0	33.81050845660357	0.0
35	0.0066003815236926645	0.0	0.0	34.44749937512781	0.0
36	0.006708584499490905	0.0	0.0	35.054301663404345	0.0
37	0.006924990451087386	0.0	0.0	35.63935515354543	0.0
38	0.006924990451087386	0.0	0.0	36.22062153953358	0.0
39	0.007033193426885626	0.0	0.0	36.82039063438323	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCACG	35	1.2115743E-7	45.000004	1
TGCGATT	35	1.2115743E-7	45.000004	27
GCTACGA	35	1.2115743E-7	45.000004	10
ATACCGG	30	2.1650376E-6	45.000004	1
CTATCGA	20	7.032569E-4	45.0	27
GCTATCG	25	3.890211E-5	45.0	26
ACCGAAC	20	7.032569E-4	45.0	35
TTCGTTA	25	3.890211E-5	45.0	13
CCTCGAT	45	3.8380676E-10	45.0	15
ACCTCGT	20	7.032569E-4	45.0	45
CGTTAAC	25	3.890211E-5	45.0	15
CCGTATT	20	7.032569E-4	45.0	16
AATGCGC	50	2.1827873E-11	45.0	8
AATGCGA	20	7.032569E-4	45.0	13
TACGCGG	50	2.1827873E-11	45.0	2
CCGTAAC	20	7.032569E-4	45.0	30
CGCTAGC	20	7.032569E-4	45.0	22
AACTCGC	25	3.890211E-5	45.0	39
ATAGTCG	25	3.890211E-5	45.0	11
CACGTAA	25	3.890211E-5	45.0	43
>>END_MODULE