SRR3552796_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552796_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	398465
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	2796	0.7016927459124389	Illumina Paired End PCR Primer 2 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT	2021	0.5071963660547351	TruSeq Adapter, Index 1 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	2004	0.5029299938514048	Illumina Paired End PCR Primer 2 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	930	0.23339565582924474	Illumina Paired End PCR Primer 2 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	578	0.1450566549132295	No Hit
ACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	555	0.13928450428519445	TruSeq Adapter, Index 1 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTG	526	0.13200657523245454	Illumina Paired End PCR Primer 2 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCC	464	0.11644686484383823	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	431	0.1081650835079618	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	410	0.10289485902149498	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGG	30	2.161918E-6	45.000004	2
TACGTGG	30	2.161918E-6	45.000004	2
TCGTTTG	25	3.8862025E-5	45.0	1
TCGTTCG	25	3.8862025E-5	45.0	45
TCGTTAA	20	7.027732E-4	45.0	14
GAGTCGA	20	7.027732E-4	45.0	25
GGCACGC	25	3.8862025E-5	45.0	9
TAAACGG	20	7.027732E-4	45.0	2
TTCGTTA	20	7.027732E-4	45.0	13
CCTCGAT	20	7.027732E-4	45.0	15
CGTTAAC	20	7.027732E-4	45.0	15
AATGCGC	35	1.2092642E-7	45.0	8
AGACGCA	20	7.027732E-4	45.0	24
GCACATA	20	7.027732E-4	45.0	9
AAGCATA	20	7.027732E-4	45.0	25
TACTGGC	25	3.8862025E-5	45.0	3
TCGTCGG	20	7.027732E-4	45.0	12
GCCTCGA	20	7.027732E-4	45.0	14
GTGTACG	20	7.027732E-4	45.0	1
TCGTAAG	20	7.027732E-4	45.0	1