FastQCFastQC Report
Sat 18 Jun 2016
SRR3552795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777639
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC84540.47557462454412847TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC78750.4430033319476002TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCT77940.4384467262475677TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC41870.23553713661772724TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC25570.1438424787034938TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCC21250.11954058163665401No Hit
CGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTG17870.10052659735750621No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG207.034329E-445.01
TCCAACG207.034329E-445.038
GTATACG207.034329E-445.01
TACGTAA207.034329E-445.01
CTTACCG253.8916696E-545.01
TTAACGG1500.042.0000042
TCGACGT650.041.53846426
TACGCAT603.6379788E-1241.24999616
CGCATCG556.184564E-1140.90909210
TAACGCG800.039.3751
TGCGACG403.459645E-739.3751
TAGTACG1200.039.3749961
CGCGTTC356.2502168E-638.5714269
GCCGTAA356.2502168E-638.57142617
GCTACGA2650.038.20754610
CTACGAA2550.037.94117411
TACGAAT2500.037.812
TTCGTAG900.037.51
TCTACGG900.037.52
ACGTACG900.037.51