##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552795_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1777639
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.704733075725724	31.0	31.0	34.0	30.0	34.0
2	31.862726909119342	33.0	31.0	34.0	30.0	34.0
3	31.905974160107874	33.0	31.0	34.0	30.0	34.0
4	35.53026401873496	37.0	35.0	37.0	33.0	37.0
5	35.415576503440796	37.0	35.0	37.0	33.0	37.0
6	35.39085157335094	37.0	35.0	37.0	33.0	37.0
7	35.82179565142304	37.0	35.0	37.0	35.0	37.0
8	35.81111519267973	37.0	35.0	37.0	35.0	37.0
9	37.41790431015521	39.0	37.0	39.0	35.0	39.0
10	36.83056121068451	39.0	37.0	39.0	32.0	39.0
11	36.59778728977031	39.0	35.0	39.0	32.0	39.0
12	36.545551712130525	39.0	35.0	39.0	32.0	39.0
13	36.53015038486442	39.0	35.0	39.0	32.0	39.0
14	37.59310467423363	39.0	36.0	41.0	33.0	41.0
15	37.64510229579797	39.0	36.0	41.0	33.0	41.0
16	37.710861991664224	39.0	36.0	41.0	33.0	41.0
17	37.70208855678796	39.0	36.0	41.0	33.0	41.0
18	37.657328062671894	39.0	36.0	41.0	33.0	41.0
19	37.6334919519655	39.0	36.0	41.0	32.0	41.0
20	37.58386320282127	39.0	36.0	41.0	32.0	41.0
21	37.5264184685417	39.0	36.0	41.0	32.0	41.0
22	37.4841663577363	39.0	36.0	41.0	32.0	41.0
23	37.44206950905105	39.0	36.0	41.0	32.0	41.0
24	37.42503174154032	39.0	36.0	41.0	32.0	41.0
25	37.3819999448707	39.0	36.0	41.0	32.0	41.0
26	37.28086748771826	39.0	36.0	41.0	32.0	41.0
27	37.22618484405439	39.0	35.0	41.0	32.0	41.0
28	37.16340831856187	39.0	35.0	41.0	32.0	41.0
29	37.143045353977946	39.0	35.0	41.0	31.0	41.0
30	37.06168744047582	39.0	35.0	41.0	31.0	41.0
31	36.98219773531071	39.0	35.0	40.0	31.0	41.0
32	36.85880935330514	39.0	35.0	40.0	31.0	41.0
33	36.758551089394416	39.0	35.0	40.0	31.0	41.0
34	36.64789813904848	39.0	35.0	40.0	30.0	41.0
35	36.61339394556487	39.0	35.0	40.0	30.0	41.0
36	36.54527437798113	39.0	35.0	40.0	30.0	41.0
37	36.499854019854425	39.0	35.0	40.0	30.0	41.0
38	36.450565047234	39.0	35.0	40.0	30.0	41.0
39	36.42068608980789	39.0	35.0	40.0	30.0	41.0
40	36.31466568859032	38.0	35.0	40.0	30.0	41.0
41	36.22564311426561	38.0	35.0	40.0	30.0	41.0
42	36.11994167544704	38.0	35.0	40.0	30.0	41.0
43	36.01230508556574	38.0	35.0	40.0	30.0	41.0
44	35.89696164406834	38.0	35.0	40.0	29.0	41.0
45	35.874405883309265	38.0	35.0	40.0	29.0	41.0
46	35.75438770188998	38.0	34.0	40.0	29.0	41.0
47	35.66197917574941	38.0	34.0	40.0	28.0	41.0
48	35.57813931850055	38.0	34.0	40.0	28.0	41.0
49	35.52432411755142	38.0	34.0	40.0	28.0	41.0
50	35.404291309990384	38.0	34.0	40.0	27.0	41.0
51	33.786032484660836	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	4.0
11	7.0
12	19.0
13	22.0
14	17.0
15	62.0
16	116.0
17	279.0
18	640.0
19	1059.0
20	1824.0
21	2663.0
22	3979.0
23	5612.0
24	7879.0
25	10778.0
26	13932.0
27	17506.0
28	22193.0
29	28856.0
30	36849.0
31	47732.0
32	61943.0
33	83032.0
34	125320.0
35	146897.0
36	163975.0
37	228453.0
38	360189.0
39	405727.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44335379680576	20.66133787568792	24.807005246847083	11.088303080659234
2	29.739052754805673	25.343672140406458	26.483667381284953	18.433607723502917
3	31.711387970223427	25.1299617076358	27.07248209563359	16.08616822650718
4	28.161904638680856	27.079682657727467	25.596816901519375	19.1615958020723
5	25.094802713036785	31.158632320735535	24.508294428733844	19.238270537493833
6	27.17891540408373	34.61068304644531	23.03932350719128	15.171078042279676
7	82.48187624146411	5.466914260994499	7.370787882129049	4.680421615412353
8	83.0597776038892	4.413382019633907	7.2753241799938	5.251516196483088
9	77.39254145526735	7.028929945843898	9.805252922556267	5.773275676332483
10	40.220820987838366	31.038529195185298	15.433336014792653	13.307313802183682
11	26.856127706469085	26.40012961011769	26.70778487645692	20.035957806956304
12	25.620725017846706	23.21894377879873	30.591138020711746	20.56919318264282
13	24.17689980924136	24.45333388837666	31.598485406767068	19.771280895614915
14	19.944881947347014	29.007970684711577	28.957904276402573	22.089243091538833
15	19.364336628528065	27.578827872250777	32.79676019709289	20.26007530212827
16	21.120092437215877	27.092396150174476	30.778296380761223	21.009215031848424
17	21.471457365640607	26.1731993953778	28.5898317937444	23.765511445237195
18	22.19950169860135	25.755904320281005	30.104200009113214	21.94039397200444
19	22.9506665864104	27.394538486160574	28.078141849948164	21.576653077480863
20	25.456630958254177	26.744912774753477	28.402448416129484	19.39600785086286
21	23.99570441467587	27.066519130149597	28.76495171404318	20.172824741131354
22	21.319289237016065	26.32390490982702	27.123111047856174	25.23369480530074
23	21.141356597149365	27.76418609177679	27.662196880243968	23.43226043082988
24	22.376140487466802	25.2721728089899	28.567948835505973	23.783737868037324
25	20.765521008483727	28.13034592512878	26.41183052352024	24.692302542867253
26	20.40729304431327	29.713175734780794	27.314657250431612	22.56487397047432
27	21.33228400141986	29.499634065184217	27.721151482387597	21.44693045100833
28	18.941022333555914	28.16421106872655	29.672447555437298	23.222319042280237
29	21.107378944768875	27.133349347083406	27.30109994211423	24.458171766033484
30	23.165614615790943	27.748378607805073	26.128758426204644	22.95724835019934
31	21.743728619815382	29.01173972893259	25.007495897648514	24.237035753603518
32	22.118776647002004	29.07108811181573	25.551532116475844	23.258603124706422
33	23.627069388104108	27.45152418460666	25.35925460681274	23.562151820476483
34	20.093449794924616	26.84662071432951	27.700731138324485	25.359198352421387
35	20.833701330810136	27.33305243640582	26.233897883653544	25.5993483491305
36	22.643461355202042	29.581090423871213	26.06423463931653	21.711213581610213
37	20.81569992557544	27.696005769450377	29.17510248143746	22.313191823536723
38	21.414134140846368	26.8312070110973	27.295643266152464	24.459015581903863
39	25.08816469485649	23.507697569641532	26.811574228513212	24.592563506988764
40	23.44840544114975	24.029569558273643	28.848939520341304	23.6730854802353
41	20.229810439577438	26.51618241949012	27.83456033536618	25.41944680556626
42	22.827581978118168	26.03239465380766	28.127308187995425	23.012715180078743
43	23.465956811253577	24.973293227702587	28.3104162318671	23.250333729176734
44	21.992373029619625	24.83209470539294	27.487977030206924	25.68755523478051
45	22.983125370224215	23.45301830124114	26.527827078501314	27.036029250033334
46	22.44960872258091	25.554963634348702	27.11714808237218	24.878279560698207
47	19.98757903038806	25.493815110942098	30.603795258767384	23.914810599902456
48	20.90430059196496	24.194563688127904	29.830016105632247	25.071119614274888
49	22.52695851069874	23.22147522640986	30.31498521353323	23.936581049358168
50	21.489402516483942	22.819650108936628	29.091508455878838	26.599438918700592
51	20.927308638030556	22.206139716781642	26.57164924936953	30.294902395818273
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	662.0
1	1021.5
2	1381.0
3	2027.0
4	2673.0
5	2147.5
6	1622.0
7	1713.5
8	1805.0
9	2048.5
10	2292.0
11	2433.5
12	2575.0
13	2887.5
14	3200.0
15	3158.5
16	3117.0
17	3464.5
18	3812.0
19	4130.0
20	4448.0
21	5026.0
22	5604.0
23	6314.5
24	7025.0
25	9431.0
26	13680.5
27	15524.0
28	19135.0
29	22746.0
30	25731.5
31	28717.0
32	32392.5
33	36068.0
34	42314.0
35	48560.0
36	51715.0
37	54870.0
38	59517.5
39	64165.0
40	72395.5
41	80626.0
42	88796.0
43	96966.0
44	109317.0
45	121668.0
46	141580.0
47	161492.0
48	184975.0
49	208458.0
50	201982.5
51	195507.0
52	167325.0
53	139143.0
54	119545.0
55	99947.0
56	86888.0
57	73829.0
58	70295.5
59	66762.0
60	64152.0
61	61542.0
62	55575.0
63	49608.0
64	41380.5
65	33153.0
66	26520.5
67	19888.0
68	17194.5
69	14501.0
70	12622.0
71	10743.0
72	8975.0
73	7207.0
74	6368.5
75	4896.5
76	4263.0
77	3012.0
78	1761.0
79	1314.5
80	868.0
81	727.5
82	587.0
83	503.5
84	420.0
85	335.5
86	251.0
87	178.5
88	106.0
89	74.0
90	42.0
91	33.0
92	24.0
93	21.0
94	18.0
95	21.5
96	25.0
97	12.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1777639.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.753619485494646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.72262749092033	15.373966655207367
2	13.630614472226648	6.475528590534964
3	6.1481942606761315	4.3812560097300866
4	3.372430503712373	3.20429723705835
5	1.9801811697094285	2.3518235008809727
6	1.311922408177854	1.869774340701032
7	0.8800815326568163	1.463358529005866
8	0.6752151816689087	1.2831043596953915
9	0.5169102600068555	1.1050640661915851
>10	5.18757322360655	30.930754369490003
>50	1.4196289765510985	22.298820353489614
>100	0.1442019898530915	5.639964384788116
>500	0.006866758563223956	1.1054383490235722
>1k	0.0028414173365064648	1.1600522004991949
>5k	7.103543341266162E-4	1.3567970537038585
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	8454	0.47557462454412847	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	7875	0.4430033319476002	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCT	7794	0.4384467262475677	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	4187	0.23553713661772724	TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	2557	0.1438424787034938	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCC	2125	0.11954058163665401	No Hit
CGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTG	1787	0.10052659735750621	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.45982339496376934	0.0
2	0.0	0.0	0.0	1.8292240438019194	0.0
3	0.0	0.0	0.0	2.4387403741704587	0.0
4	0.0	0.0	0.0	3.1065925083776853	0.0
5	0.0	0.0	0.0	4.8301145508171235	0.0
6	0.0	0.0	0.0	5.680793456939233	0.0
7	0.0	0.0	0.0	6.648762769043659	0.0
8	0.0	0.0	0.0	8.262757511508243	0.0
9	0.0	0.0	0.0	8.790198684884839	0.0
10	0.0	0.0	0.0	10.19993373232698	0.0
11	5.625439135842542E-5	0.0	0.0	12.471261037814765	0.0
12	5.625439135842542E-5	0.0	0.0	14.244343199040975	0.0
13	5.625439135842542E-5	0.0	0.0	14.887105874702344	0.0
14	5.625439135842542E-5	0.0	0.0	15.141825758773294	0.0
15	5.625439135842542E-5	0.0	0.0	15.619200523840892	0.0
16	5.625439135842542E-5	0.0	0.0	16.689834100174444	0.0
17	1.1250878271685083E-4	0.0	0.0	18.02441328076173	0.0
18	1.1250878271685083E-4	0.0	0.0	19.4410113639496	0.0
19	1.1250878271685083E-4	0.0	0.0	20.340237809814028	0.0
20	1.6876317407527624E-4	0.0	0.0	21.13578741240488	0.0
21	5.625439135842541E-4	0.0	0.0	22.13824066641202	0.0
22	6.75052696301105E-4	0.0	0.0	23.231094727332152	0.0
23	7.875614790179558E-4	0.0	0.0	24.23540437625412	0.0
24	0.0011813422185269337	0.0	0.0	25.0371419618944	0.0
25	0.0037690442210145027	0.0	0.0	25.69807480596454	0.0
26	0.0038252986123729284	0.0	0.0	26.325705050350493	0.0
27	0.003937807395089779	0.0	0.0	26.931114810149868	0.0
28	0.003937807395089779	0.0	0.0	27.556832405229635	0.0
29	0.003937807395089779	0.0	0.0	28.234754075490017	0.0
30	0.003937807395089779	0.0	0.0	28.96561112801868	0.0
31	0.003937807395089779	0.0	0.0	29.651745939417395	0.0
32	0.00405031617780663	0.0	0.0	30.311272423703574	0.0
33	0.004106570569165056	0.0	0.0	30.96528597763663	0.0
34	0.004275333743240331	0.0	0.0	31.584365554536102	0.0
35	0.004612860091390884	0.0	0.0	32.237366529424705	0.0
36	0.004612860091390884	0.0	0.0	32.91236297133445	0.0
37	0.004837877656824586	0.0	0.0	33.56603899891935	0.0
38	0.004837877656824586	0.0	0.0	34.20593270062144	0.0
39	0.004837877656824586	0.0	0.0	34.83901962096916	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.034329E-4	45.0	1
TCCAACG	20	7.034329E-4	45.0	38
GTATACG	20	7.034329E-4	45.0	1
TACGTAA	20	7.034329E-4	45.0	1
CTTACCG	25	3.8916696E-5	45.0	1
TTAACGG	150	0.0	42.000004	2
TCGACGT	65	0.0	41.538464	26
TACGCAT	60	3.6379788E-12	41.249996	16
CGCATCG	55	6.184564E-11	40.909092	10
TAACGCG	80	0.0	39.375	1
TGCGACG	40	3.459645E-7	39.375	1
TAGTACG	120	0.0	39.374996	1
CGCGTTC	35	6.2502168E-6	38.571426	9
GCCGTAA	35	6.2502168E-6	38.571426	17
GCTACGA	265	0.0	38.207546	10
CTACGAA	255	0.0	37.941174	11
TACGAAT	250	0.0	37.8	12
TTCGTAG	90	0.0	37.5	1
TCTACGG	90	0.0	37.5	2
ACGTACG	90	0.0	37.5	1
>>END_MODULE