FastQCFastQC Report
Sat 18 Jun 2016
SRR3552794_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552794_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757685
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC44530.5877112520374562TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC35390.4670806469707068TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCT33850.44675557784567466TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC15180.2003471099467457TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC9630.12709767251562323TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8800.11614325214304097No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCC8770.11574730923800788No Hit
CGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTG7680.10136138368847213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATG406.8102963E-945.0000041
ATAGCCG207.031762E-445.0000041
CGTAGCG207.031762E-445.0000042
TATACGG207.031762E-445.0000042
GCGCTAG207.031762E-445.0000041
ATCGAGC207.031762E-445.00000432
TGCGTAG406.8102963E-945.0000041
AACTGCG253.889542E-545.01
ACGTACG253.889542E-545.01
CGCATGG650.041.538462
TTTGACG603.6379788E-1241.2500041
TTAACGG501.0804797E-940.52
AACACGT1500.040.541
CGACCAA1700.039.70588329
TATTGCG403.4561344E-739.3750041
TCATACG403.4561344E-739.3750041
GACACGA1800.038.7525
TTAGGCG700.038.571431
GCGATTA1050.038.571439
ACCGTTG356.245469E-638.571431