##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552794_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	757685
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.980555243933825	33.0	33.0	33.0	27.0	33.0
2	32.08208028402304	33.0	33.0	33.0	33.0	33.0
3	32.13754000673103	33.0	33.0	33.0	33.0	33.0
4	32.08499706342345	33.0	33.0	33.0	33.0	33.0
5	32.12499257607053	33.0	33.0	33.0	33.0	33.0
6	35.6119891511644	37.0	37.0	37.0	33.0	37.0
7	35.97059464025288	37.0	37.0	37.0	37.0	37.0
8	36.01643163055887	37.0	37.0	37.0	37.0	37.0
9	35.89612041943552	37.0	37.0	37.0	37.0	37.0
10	35.866890594376294	37.0	37.0	37.0	33.0	37.0
11	35.82259646159024	37.0	37.0	37.0	33.0	37.0
12	35.82604380448339	37.0	37.0	37.0	33.0	37.0
13	35.86085642450359	37.0	37.0	37.0	33.0	37.0
14	35.90332658030712	37.0	37.0	37.0	37.0	37.0
15	35.919779327820926	37.0	37.0	37.0	37.0	37.0
16	35.93294046998423	37.0	37.0	37.0	37.0	37.0
17	35.93527917274329	37.0	37.0	37.0	37.0	37.0
18	35.92653807320984	37.0	37.0	37.0	37.0	37.0
19	35.91897424391403	37.0	37.0	37.0	37.0	37.0
20	35.897789978685076	37.0	37.0	37.0	37.0	37.0
21	35.80357404462276	37.0	37.0	37.0	33.0	37.0
22	35.85764796716313	37.0	37.0	37.0	33.0	37.0
23	35.877504503850545	37.0	37.0	37.0	37.0	37.0
24	35.87947102027888	37.0	37.0	37.0	37.0	37.0
25	35.882977754607786	37.0	37.0	37.0	37.0	37.0
26	35.84545160587843	37.0	37.0	37.0	33.0	37.0
27	35.83038069910319	37.0	37.0	37.0	33.0	37.0
28	35.81586675201436	37.0	37.0	37.0	33.0	37.0
29	35.807896421336046	37.0	37.0	37.0	33.0	37.0
30	35.811437470716726	37.0	37.0	37.0	33.0	37.0
31	35.79652890053254	37.0	37.0	37.0	33.0	37.0
32	35.77588047803507	37.0	37.0	37.0	33.0	37.0
33	35.74027069296607	37.0	37.0	37.0	33.0	37.0
34	35.714504048516204	37.0	37.0	37.0	33.0	37.0
35	35.69981852616853	37.0	37.0	37.0	33.0	37.0
36	35.692395916508836	37.0	37.0	37.0	33.0	37.0
37	35.70365653272798	37.0	37.0	37.0	33.0	37.0
38	35.68053082745468	37.0	37.0	37.0	33.0	37.0
39	35.66864990068432	37.0	37.0	37.0	33.0	37.0
40	35.66692358961838	37.0	37.0	37.0	33.0	37.0
41	35.65922381992517	37.0	37.0	37.0	33.0	37.0
42	35.63865854543775	37.0	37.0	37.0	33.0	37.0
43	35.63016952955384	37.0	37.0	37.0	33.0	37.0
44	35.591382962576795	37.0	37.0	37.0	33.0	37.0
45	35.399864059602606	37.0	37.0	37.0	33.0	37.0
46	35.532510212027425	37.0	37.0	37.0	33.0	37.0
47	35.53950652315936	37.0	37.0	37.0	33.0	37.0
48	35.56473864468744	37.0	37.0	37.0	33.0	37.0
49	35.55483611263256	37.0	37.0	37.0	33.0	37.0
50	35.55333284940312	37.0	37.0	37.0	33.0	37.0
51	35.218586879771934	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	516.0
15	1913.0
16	1359.0
17	781.0
18	568.0
19	560.0
20	656.0
21	709.0
22	863.0
23	1183.0
24	1709.0
25	2468.0
26	3482.0
27	5254.0
28	7177.0
29	8923.0
30	10789.0
31	13977.0
32	18163.0
33	25227.0
34	39018.0
35	83528.0
36	528862.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.919023076872314	19.082204346133288	24.566673485683367	13.432099091311034
2	29.18600737773613	24.334123019460595	26.27582702574289	20.204042577060388
3	32.210747210252286	24.37213353834377	26.13473937058276	17.282379880821185
4	28.952533044734952	26.2615730811617	25.03032262747712	19.755571246626236
5	25.376112764539354	30.560721144011033	24.386651444861652	19.67651464658796
6	26.871721097817698	34.744649821495734	22.534694497053525	15.84893458363304
7	81.72631106594429	5.881731854266615	7.536113292463227	4.855843787325868
8	82.75576261903034	4.648237724120182	7.166434600130661	5.429565056718822
9	77.2833037475996	7.120241261210134	9.88537452899292	5.7110804621973505
10	40.4414763391119	30.834053729452215	15.456687145713587	13.267782785722298
11	27.12010928024179	26.276486930584607	26.73485683364459	19.868546955529013
12	25.75331437206755	23.16463965896118	30.66023479414268	20.421811174828587
13	24.442743356408005	24.16043606511941	31.554009911770724	19.842810666701862
14	20.335759583468064	28.38356309020239	28.9806449909923	22.300032335337246
15	19.552848479249292	27.28099408065357	32.673472485267624	20.492684954829514
16	21.47013600638788	26.483433088948573	30.88169885902453	21.164732045639017
17	21.726047104007602	25.724542520968473	28.822795752852436	23.726614622171482
18	22.309402984089694	25.16454727228334	30.40999887816177	22.1160508654652
19	23.00652645888463	27.063885387727087	28.426192942977625	21.50339521041066
20	25.74790315236543	26.322944231441824	28.51158462949643	19.41756798669632
21	24.1711265235553	26.786725354203924	28.77541458521681	20.266733537023963
22	21.419719276480333	26.180140823693222	27.29353227264629	25.10660762718016
23	21.1019091047071	27.61266225410296	27.65542408784653	23.630004553343408
24	22.193391712915	25.181176874294724	28.639210225885424	23.98622118690485
25	20.679833967941825	27.70161742676706	26.767324151857302	24.851224453433815
26	20.421283250955213	29.28129763688076	27.70848043712097	22.588938675043057
27	21.00305535941717	29.13770234332209	28.241023644390477	21.618218652870254
28	19.066234648963622	27.65106871589117	30.080310419237545	23.202386215907666
29	21.076568758784983	26.72403439424035	27.58982954657938	24.609567300395284
30	23.246335878366338	27.204973042887215	26.48488487960036	23.063806199146082
31	21.938008539168653	28.58338227627576	25.12086157176135	24.357747612794235
32	22.15630506081023	28.711139853633107	25.872757148419197	23.259797937137463
33	23.55543530622884	27.252750153427876	25.661719580036557	23.530094960306723
34	20.01095442037258	26.78105017256512	28.045823792209163	25.16217161485314
35	20.792149772002876	27.384467159835552	26.364518236470303	25.45886483169127
36	22.571385206253257	29.35467905528023	26.299847561981558	21.774088176484952
37	20.869490619452673	27.444122557527205	29.15208826887163	22.534298554148492
38	21.387251958267615	26.750166625972533	27.45098556788111	24.411595847878736
39	24.831955232055538	23.52455175963626	26.901020872790145	24.74247213551806
40	23.40128153520262	23.952830001913725	28.833354230319983	23.812534232563664
41	20.31899800048833	26.189775434382362	27.877548057570102	25.61367850755921
42	22.867550499218012	25.87830034908966	28.218058955898563	23.036090195793765
43	23.362215168572693	24.827863822036864	28.470538548341327	23.339382461049116
44	21.88693190441938	24.7872136837868	27.858938741033544	25.466915670760276
45	22.87322568085682	23.795640668615587	26.626236496697175	26.70489715383042
46	22.221767621109038	25.50690590416862	27.46299583600045	24.808330638721895
47	19.94588780297881	25.50189062737153	30.51202016669196	24.040201402957692
48	21.02786778146591	24.15014154958855	29.933679563406955	24.888311105538584
49	22.65677689277206	23.175462098365415	30.243966819984557	23.923794188877963
50	21.47237968284973	23.00045533434079	28.890765951549785	26.636399031259693
51	20.85536865583983	22.25991012096056	26.75782152213651	30.12689970106311
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	234.0
1	349.5
2	465.0
3	1121.0
4	1777.0
5	1395.5
6	1014.0
7	972.0
8	930.0
9	1091.5
10	1253.0
11	1257.0
12	1261.0
13	1352.5
14	1444.0
15	1492.0
16	1540.0
17	1617.0
18	1694.0
19	1817.5
20	1941.0
21	2142.0
22	2343.0
23	2683.0
24	3023.0
25	3925.0
26	5650.0
27	6473.0
28	7757.0
29	9041.0
30	10177.0
31	11313.0
32	12896.0
33	14479.0
34	17094.5
35	19710.0
36	21122.5
37	22535.0
38	24534.5
39	26534.0
40	29752.5
41	32971.0
42	36721.0
43	40471.0
44	45298.0
45	50125.0
46	58360.0
47	66595.0
48	76703.5
49	86812.0
50	86170.5
51	85529.0
52	73600.0
53	61671.0
54	52727.0
55	43783.0
56	38284.0
57	32785.0
58	31134.0
59	29483.0
60	28293.0
61	27103.0
62	24754.5
63	22406.0
64	18521.0
65	14636.0
66	11983.0
67	9330.0
68	7941.0
69	6552.0
70	5591.0
71	4630.0
72	3983.0
73	3336.0
74	2834.5
75	1988.5
76	1644.0
77	1201.0
78	758.0
79	550.0
80	342.0
81	276.0
82	210.0
83	208.0
84	206.0
85	147.5
86	89.0
87	68.5
88	48.0
89	26.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	757685.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.134319630950408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.37810492806874	21.289057745891576
2	12.403644443366694	7.723580613768812
3	4.749081428653193	4.435782574592973
4	2.3614085518144203	2.940833945257995
5	1.44528808008088	2.2499030522020385
6	0.9739153872990424	1.8193315777001544
7	0.7341631190489646	1.600036844680817
8	0.5988202750014622	1.4915089474711314
9	0.5543871405938751	1.5534419791084515
>10	7.642686530490178	47.47331674123412
>50	0.10381543973043136	2.154017307189956
>100	0.049985211521103676	2.8394348098587545
>500	0.0029905682106643222	0.7145474211159053
>1k	0.0017088961203796126	1.7152064399273266
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	4453	0.5877112520374562	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	3539	0.4670806469707068	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCT	3385	0.44675557784567466	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	1518	0.2003471099467457	TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	963	0.12709767251562323	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	880	0.11614325214304097	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCC	877	0.11574730923800788	No Hit
CGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTG	768	0.10136138368847213	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3198096834436473E-4	0.0	0.0	0.4598216937117668	0.0
2	1.3198096834436473E-4	0.0	0.0	1.8795409701920982	0.0
3	1.3198096834436473E-4	0.0	0.0	2.4552419541102175	0.0
4	1.3198096834436473E-4	0.0	0.0	3.0540396074886003	0.0
5	1.3198096834436473E-4	0.0	0.0	4.529322871641909	0.0
6	1.3198096834436473E-4	0.0	0.0	5.271847799547305	0.0
7	1.3198096834436473E-4	0.0	0.0	6.159683773599847	0.0
8	1.3198096834436473E-4	0.0	0.0	7.746490956004144	0.0
9	1.3198096834436473E-4	0.0	0.0	8.308069976309417	0.0
10	1.3198096834436473E-4	0.0	0.0	9.766459676514646	0.0
11	1.3198096834436473E-4	0.0	0.0	12.072695117364075	0.0
12	1.3198096834436473E-4	0.0	0.0	13.847311217722405	0.0
13	1.3198096834436473E-4	0.0	0.0	14.46735780700423	0.0
14	1.3198096834436473E-4	0.0	0.0	14.729867953041172	0.0
15	1.3198096834436473E-4	0.0	0.0	15.193385113866581	0.0
16	1.3198096834436473E-4	0.0	0.0	16.2456693744762	0.0
17	1.3198096834436473E-4	0.0	0.0	17.539874750061042	0.0
18	1.3198096834436473E-4	0.0	0.0	18.963025531718326	0.0
19	1.3198096834436473E-4	0.0	0.0	19.87752166137643	0.0
20	1.3198096834436473E-4	0.0	0.0	20.679701986973477	0.0
21	5.279238733774589E-4	0.0	0.0	21.669163306651182	0.0
22	6.599048417218238E-4	0.0	0.0	22.727650672772985	0.0
23	7.918858100661885E-4	0.0	0.0	23.726746603139826	0.0
24	0.0014517906517880121	0.0	0.0	24.535525977154094	0.0
25	0.003035562271920389	0.0	0.0	25.19912628598956	0.0
26	0.0032995242086091186	0.0	0.0	25.85256406026251	0.0
27	0.0032995242086091186	0.0	0.0	26.45070180879917	0.0
28	0.0032995242086091186	0.0	0.0	27.07391594132126	0.0
29	0.0032995242086091186	0.0	0.0	27.738572097903482	0.0
30	0.0032995242086091186	0.0	0.0	28.478589387410334	0.0
31	0.0032995242086091186	0.0	0.0	29.157103545668715	0.0
32	0.0035634861452978482	0.0	0.0	29.828754693573188	0.0
33	0.0035634861452978482	0.0	0.0	30.49103519272521	0.0
34	0.003959429050330942	0.0	0.0	31.102635000032997	0.0
35	0.004091410018675307	0.0	0.0	31.753697116875745	0.0
36	0.004091410018675307	0.0	0.0	32.410698377294	0.0
37	0.004619333892052766	0.0	0.0	33.06070464638999	0.0
38	0.004619333892052766	0.0	0.0	33.685238588595524	0.0
39	0.004619333892052766	0.0	0.0	34.31782336987006	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATG	40	6.8102963E-9	45.000004	1
ATAGCCG	20	7.031762E-4	45.000004	1
CGTAGCG	20	7.031762E-4	45.000004	2
TATACGG	20	7.031762E-4	45.000004	2
GCGCTAG	20	7.031762E-4	45.000004	1
ATCGAGC	20	7.031762E-4	45.000004	32
TGCGTAG	40	6.8102963E-9	45.000004	1
AACTGCG	25	3.889542E-5	45.0	1
ACGTACG	25	3.889542E-5	45.0	1
CGCATGG	65	0.0	41.53846	2
TTTGACG	60	3.6379788E-12	41.250004	1
TTAACGG	50	1.0804797E-9	40.5	2
AACACGT	150	0.0	40.5	41
CGACCAA	170	0.0	39.705883	29
TATTGCG	40	3.4561344E-7	39.375004	1
TCATACG	40	3.4561344E-7	39.375004	1
GACACGA	180	0.0	38.75	25
TTAGGCG	70	0.0	38.57143	1
GCGATTA	105	0.0	38.57143	9
ACCGTTG	35	6.245469E-6	38.57143	1
>>END_MODULE