FastQCFastQC Report
Sat 18 Jun 2016
SRR3552792_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552792_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1831661
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC101860.5561072709415116No Hit
GCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC89430.488245368548001No Hit
CTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGCT87690.4787457941180163No Hit
TCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC44630.24365862460357018No Hit
ACTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC26900.14686123687734795No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCC23090.12606044459100238No Hit
GCCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTG19360.10569641434741471No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG406.8193913E-945.01
CCCGCTT207.0343865E-445.033
CTTACCG551.8189894E-1245.012
CTAGCGA253.8917162E-545.016
ATCCGGG1200.043.1250043
GCGCGAG2250.043.01
TTTCGCG650.041.5384641
GTAAGCG603.6379788E-1241.2500041
TAACGCG556.184564E-1140.9090921
GCGTAAG1600.040.781251
CTACGCG451.929402E-840.01
CGCGAGG4000.039.93752
CGTTAGG3000.039.7500042
GCTAGCG1250.039.6000021
GATCGCA403.4597178E-739.3759
GTCCGTG403.4597178E-739.37530
CATACGA403.4597178E-739.37518
ACGCATT356.2503186E-638.5714317
CGTAAGG2900.038.017242
TAGCGGA301.1401864E-437.5000043
Produced by FastQC (version 0.11.5)