##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552792_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1831661
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.350374878320824	33.0	33.0	33.0	27.0	33.0
2	31.561398643089525	33.0	33.0	33.0	27.0	33.0
3	31.642403807254727	33.0	33.0	33.0	27.0	33.0
4	31.555752947734323	33.0	33.0	33.0	27.0	33.0
5	31.646863147711287	33.0	33.0	33.0	27.0	33.0
6	35.06182148334217	37.0	37.0	37.0	27.0	37.0
7	35.48391705670427	37.0	37.0	37.0	33.0	37.0
8	35.58917998472425	37.0	37.0	37.0	33.0	37.0
9	35.460894237525395	37.0	37.0	37.0	33.0	37.0
10	35.4347966135655	37.0	37.0	37.0	33.0	37.0
11	35.43421681195374	37.0	37.0	37.0	33.0	37.0
12	35.464130098309674	37.0	37.0	37.0	33.0	37.0
13	35.533680631951	37.0	37.0	37.0	33.0	37.0
14	35.580157572825975	37.0	37.0	37.0	33.0	37.0
15	35.605846824275886	37.0	37.0	37.0	33.0	37.0
16	35.61466614182428	37.0	37.0	37.0	33.0	37.0
17	35.617709827309746	37.0	37.0	37.0	33.0	37.0
18	35.612283604881036	37.0	37.0	37.0	33.0	37.0
19	35.6074956009873	37.0	37.0	37.0	33.0	37.0
20	35.590423664641	37.0	37.0	37.0	33.0	37.0
21	35.51236991997973	37.0	37.0	37.0	33.0	37.0
22	35.54593508296568	37.0	37.0	37.0	33.0	37.0
23	35.55687542618421	37.0	37.0	37.0	33.0	37.0
24	35.55910291260228	37.0	37.0	37.0	33.0	37.0
25	35.54626920592839	37.0	37.0	37.0	33.0	37.0
26	35.492670859946244	37.0	37.0	37.0	33.0	37.0
27	35.487634447640694	37.0	37.0	37.0	33.0	37.0
28	35.47064767989273	37.0	37.0	37.0	33.0	37.0
29	35.47239691187398	37.0	37.0	37.0	33.0	37.0
30	35.46184037330052	37.0	37.0	37.0	33.0	37.0
31	35.45055717187842	37.0	37.0	37.0	33.0	37.0
32	35.432129089389356	37.0	37.0	37.0	33.0	37.0
33	35.41929374485781	37.0	37.0	37.0	33.0	37.0
34	35.37648287537923	37.0	37.0	37.0	33.0	37.0
35	35.35678108558297	37.0	37.0	37.0	33.0	37.0
36	35.35962386052878	37.0	37.0	37.0	33.0	37.0
37	35.353594360528504	37.0	37.0	37.0	33.0	37.0
38	35.34056574879303	37.0	37.0	37.0	33.0	37.0
39	35.34503928401598	37.0	37.0	37.0	33.0	37.0
40	35.33556755316622	37.0	37.0	37.0	33.0	37.0
41	35.3124655708671	37.0	37.0	37.0	33.0	37.0
42	35.30039455991037	37.0	37.0	37.0	33.0	37.0
43	35.29338944269709	37.0	37.0	37.0	33.0	37.0
44	35.23822803455443	37.0	37.0	37.0	33.0	37.0
45	35.21538537971819	37.0	37.0	37.0	33.0	37.0
46	35.22830698475318	37.0	37.0	37.0	33.0	37.0
47	35.21247599856087	37.0	37.0	37.0	33.0	37.0
48	35.20556642304444	37.0	37.0	37.0	33.0	37.0
49	35.19327320939846	37.0	37.0	37.0	33.0	37.0
50	35.18057981253081	37.0	37.0	37.0	33.0	37.0
51	34.77075834447532	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1172.0
15	4732.0
16	4566.0
17	3794.0
18	3660.0
19	3810.0
20	3904.0
21	4314.0
22	4979.0
23	6025.0
24	7467.0
25	9634.0
26	12935.0
27	16875.0
28	21542.0
29	26186.0
30	31901.0
31	38896.0
32	49778.0
33	67987.0
34	102766.0
35	211560.0
36	1193178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.6031012288846	19.678859789011177	24.798147692176663	11.919891289927557
2	30.516072570197217	23.164439271240695	26.41029098725146	19.90919717131063
3	32.32939938121738	24.207863791389343	26.13715092476173	17.325585902631545
4	28.761872420715406	26.670819545756558	25.50815898793499	19.059149045593042
5	25.07030504007019	30.996674602996954	25.019804428876302	18.913215928056555
6	26.775205673975698	35.55106539911043	22.94829665533087	14.725432271583006
7	81.86438429381856	5.85501356419119	7.509850348945575	4.770751793044674
8	82.98598921962088	4.581797614296532	6.876927553734015	5.555285612348573
9	77.35568972642864	7.552926005412573	9.471676254503427	5.619708013655365
10	37.86333824872616	33.05742711123947	16.39708439498357	12.682150245050803
11	24.71297909383887	26.667489235180525	28.804620505650334	19.814911165330265
12	23.95470559235579	23.591155787015175	32.435969319650304	20.01816930097873
13	23.678235219290034	24.92508166085318	33.09193131261735	18.304751807239438
14	20.6738037224137	28.04793026657225	29.871029628299123	21.407236382714924
15	19.11570973013019	27.668602432437005	33.626473457697685	19.589214379735115
16	21.370821347399982	26.447197379864505	31.075073389672	21.106907883063514
17	21.612569138066487	26.30432159662732	29.21097299118123	22.87213627412496
18	21.54405209260884	25.65245424781114	31.77891542157637	21.024578238003645
19	21.8677473615478	27.614498534390368	29.88183948885738	20.635914615204452
20	23.19796075802236	27.18952906678692	30.324224842915804	19.288285332274913
21	22.670079234094082	28.040669097611403	30.15863743345521	19.130614234839307
22	20.31702372873583	26.228379596442792	28.97599501217747	24.478601662643907
23	19.840843911619015	27.818466408358315	28.94902495603717	23.391664723985496
24	21.309456280392496	26.15625926413239	29.481219505137684	23.053064950337426
25	19.007611124547612	29.20589563243417	27.56705525749579	24.21943798552243
26	19.319295437310725	29.458617069424964	29.075030805372826	22.14705668789148
27	20.492656665179858	28.026474331221774	29.15140956760012	22.329459435998256
28	18.150411020379863	28.9311177122841	30.05900109245106	22.859470174884983
29	20.73085576424895	26.65334906404624	29.178434218995765	23.437360952709042
30	21.243778188212776	26.668854116564145	28.96802410489714	23.11934359032594
31	20.296113745938797	30.018218436708537	25.461807616147308	24.223860201205355
32	21.198518721531986	30.82912176434395	25.65310939087528	22.31925012324879
33	21.573915697282413	27.3874368674116	26.606833906492522	24.431813528813464
34	19.494655397478027	28.328549879044214	27.591950693932993	24.58484402954477
35	20.032964615177153	28.105528260960956	26.51058247131975	25.350924652542144
36	20.65087371516891	29.019452835431885	26.686870550827912	23.642802898571297
37	20.508653074995863	29.124876273502576	27.24019346374684	23.12627718775472
38	21.253332357898106	29.752721709967073	26.456478573273113	22.53746735886171
39	23.208825213835965	24.737110196701245	29.111718816964494	22.942345772498296
40	21.18383259784425	25.303645161413602	29.39244761994714	24.120074620795005
41	19.54046081671226	28.461052563765893	26.063720306323056	25.934766313198786
42	21.970386441595906	28.038103120610202	26.16985348271323	23.821656955080662
43	22.731826467888983	25.983028518923533	27.761414366523063	23.52373064666442
44	20.844359300110664	26.445777903225544	27.749403410347224	24.960459386316572
45	21.60765556508546	24.393924421604215	28.01102387395921	25.987396139351116
46	21.617264330026135	26.3102724794599	28.27739412478619	23.795069065727777
47	18.83918476180909	27.134770025676147	29.654231869325166	24.371813343189597
48	19.80715863907131	26.283138637553566	28.84922482926699	25.060477894108136
49	22.5852928025437	24.13994729374049	29.906243567996484	23.368516335719328
50	21.003231493163852	23.393520962667218	29.000453686571916	26.602793857597014
51	19.545811151736046	23.243056438937117	26.70996434383873	30.5011680654881
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	461.0
1	541.5
2	622.0
3	1904.5
4	3187.0
5	2536.5
6	1886.0
7	1869.5
8	1853.0
9	2007.0
10	2161.0
11	2353.5
12	2546.0
13	2670.5
14	2795.0
15	2969.0
16	3143.0
17	3394.5
18	3646.0
19	4135.0
20	4624.0
21	4841.0
22	5058.0
23	6481.5
24	7905.0
25	10086.0
26	13525.5
27	14784.0
28	18304.5
29	21825.0
30	25635.0
31	29445.0
32	34427.0
33	39409.0
34	45561.5
35	51714.0
36	57512.0
37	63310.0
38	68494.0
39	73678.0
40	80678.5
41	87679.0
42	97608.0
43	107537.0
44	121674.5
45	135812.0
46	155984.0
47	176156.0
48	206066.5
49	235977.0
50	230800.0
51	225623.0
52	191956.0
53	158289.0
54	132262.0
55	106235.0
56	89544.0
57	72853.0
58	63994.5
59	55136.0
60	49956.0
61	44776.0
62	39185.5
63	33595.0
64	26808.0
65	20021.0
66	15444.0
67	10867.0
68	8912.5
69	6958.0
70	5116.5
71	3275.0
72	2518.5
73	1762.0
74	1525.5
75	953.5
76	618.0
77	511.5
78	405.0
79	345.0
80	285.0
81	197.0
82	109.0
83	79.5
84	50.0
85	38.5
86	27.0
87	17.5
88	8.0
89	4.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1831661.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.202209674704616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.92433327217451	18.874140792816757
2	12.905509915625151	7.2792779319887995
3	5.834002378960263	4.935952750024885
4	3.160265301854217	3.5650585868234517
5	1.9241777168342018	2.7133031710776283
6	1.2064809207162515	2.041525673674224
7	0.8669571885659394	1.7115075887650333
8	0.6637080445620611	1.4974426748421952
9	0.46514374413459336	1.180627326086486
>10	5.243434677152963	35.79652345870962
>50	0.7208973445950337	12.805330731406864
>100	0.07669766863344789	3.8906110205306788
>500	0.005659603710529221	1.021965627964438
>1k	0.002146746235028325	1.151252425685704
>5k	3.9031749727787735E-4	0.9748521759212693
>10k+	1.9515874863893867E-4	0.5606280636819133
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	10186	0.5561072709415116	No Hit
GCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	8943	0.488245368548001	No Hit
CTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGCT	8769	0.4787457941180163	No Hit
TCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	4463	0.24365862460357018	No Hit
ACTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	2690	0.14686123687734795	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCC	2309	0.12606044459100238	No Hit
GCCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTG	1936	0.10569641434741471	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0919051068947801E-4	0.0	0.0	0.4973627761905724	0.0
2	1.0919051068947801E-4	0.0	0.0	1.9867759372503973	0.0
3	1.0919051068947801E-4	0.0	0.0	2.6046304419868087	0.0
4	1.0919051068947801E-4	0.0	0.0	3.2739136772579642	0.0
5	1.0919051068947801E-4	0.0	0.0	4.894082474868439	0.0
6	1.0919051068947801E-4	0.0	0.0	5.649298642052214	0.0
7	1.0919051068947801E-4	0.0	0.0	6.572995767229853	0.0
8	1.0919051068947801E-4	0.0	0.0	8.293401453653269	0.0
9	1.0919051068947801E-4	0.0	0.0	8.876860947522495	0.0
10	1.0919051068947801E-4	0.0	0.0	10.549823357051332	0.0
11	1.0919051068947801E-4	0.0	0.0	13.14844832095022	0.0
12	1.0919051068947801E-4	0.0	0.0	15.204287256211712	0.0
13	1.0919051068947801E-4	0.0	0.0	15.933024724553288	0.0
14	1.0919051068947801E-4	0.0	0.0	16.195627902761483	0.0
15	1.0919051068947801E-4	0.0	0.0	16.716139067218226	0.0
16	1.0919051068947801E-4	0.0	0.0	17.84626085285432	0.0
17	1.0919051068947801E-4	0.0	0.0	19.422262088890903	0.0
18	1.0919051068947801E-4	0.0	0.0	21.031839406964497	0.0
19	1.0919051068947801E-4	0.0	0.0	22.114572510961363	0.0
20	1.6378576603421702E-4	0.0	0.0	23.10263744219045	0.0
21	3.2757153206843404E-4	0.0	0.0	24.262022284691326	0.0
22	4.913572981026511E-4	0.0	0.0	25.606430447555525	0.0
23	6.005478087921291E-4	0.0	0.0	26.84252162381576	0.0
24	9.281193408605631E-4	0.0	0.0	27.77719239531769	0.0
25	0.0014740718943079532	0.0	0.0	28.617194994051847	0.0
26	0.0015286671496526922	0.0	0.0	29.418871723533993	0.0
27	0.0015286671496526922	0.0	0.0	30.151048692962288	0.0
28	0.0015832624049974312	0.0	0.0	30.92995920096568	0.0
29	0.0015832624049974312	0.0	0.0	31.73496624102386	0.0
30	0.0015832624049974312	0.0	0.0	32.63393171553033	0.0
31	0.0015832624049974312	0.0	0.0	33.447018853379525	0.0
32	0.0015832624049974312	0.0	0.0	34.2349375785148	0.0
33	0.0016924529156869092	0.0	0.0	34.9774876464586	0.0
34	0.0016924529156869092	0.0	0.0	35.734341671302715	0.0
35	0.0016924529156869092	0.0	0.0	36.498402269852335	0.0
36	0.0016924529156869092	0.0	0.0	37.27283596691746	0.0
37	0.0016924529156869092	0.0	0.0	38.01352979617953	0.0
38	0.0018016434263763872	0.0	0.0	38.75356848237747	0.0
39	0.0019654291924106043	0.0	0.0	39.44878446393738	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTACG	40	6.8193913E-9	45.0	1
CCCGCTT	20	7.0343865E-4	45.0	33
CTTACCG	55	1.8189894E-12	45.0	12
CTAGCGA	25	3.8917162E-5	45.0	16
ATCCGGG	120	0.0	43.125004	3
GCGCGAG	225	0.0	43.0	1
TTTCGCG	65	0.0	41.538464	1
GTAAGCG	60	3.6379788E-12	41.250004	1
TAACGCG	55	6.184564E-11	40.909092	1
GCGTAAG	160	0.0	40.78125	1
CTACGCG	45	1.929402E-8	40.0	1
CGCGAGG	400	0.0	39.9375	2
CGTTAGG	300	0.0	39.750004	2
GCTAGCG	125	0.0	39.600002	1
GATCGCA	40	3.4597178E-7	39.375	9
GTCCGTG	40	3.4597178E-7	39.375	30
CATACGA	40	3.4597178E-7	39.375	18
ACGCATT	35	6.2503186E-6	38.57143	17
CGTAAGG	290	0.0	38.01724	2
TAGCGGA	30	1.1401864E-4	37.500004	3
>>END_MODULE