FastQCFastQC Report
Sat 18 Jun 2016
SRR3552790_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552790_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504835
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC40780.80778868343122TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC23960.4746105163073083TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT21460.42508938564085297TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC12010.2378995117216516TruSeq Adapter, Index 13 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7910.1566848574286648No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA7410.14678063129537375No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7280.14420553250071805No Hit
ACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC6820.13509364445809027TruSeq Adapter, Index 16 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTG6610.13093386948210803No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC6430.12736834807412323No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTG5710.11310626244218408No Hit
ACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTG5490.10874840294353601No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA207.029524E-445.037
CTATGCG253.8876868E-545.01
TCGTTCA253.8876868E-545.016
TCCGCGC207.029524E-445.013
GATCGTG207.029524E-445.01
CTCAACG207.029524E-445.01
TCCGATA207.029524E-445.07
TTCACCG351.2101191E-745.023
ACCGAAA207.029524E-445.016
ATTCGGA207.029524E-445.034
GCGCATT207.029524E-445.07
TCTAGCG351.2101191E-745.01
ATCCGAT207.029524E-445.06
AAACGGA207.029524E-445.023
TGCGCAC207.029524E-445.023
CATAGCG207.029524E-445.02
TGCGAAG207.029524E-445.01
GCCCTAT207.029524E-445.018
ACTACGG253.8876868E-545.01
AATCTCC207.029524E-445.044