##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552790_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	504835
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53828280527301	33.0	33.0	33.0	27.0	33.0
2	31.742755553794805	33.0	33.0	33.0	27.0	33.0
3	31.789245991264472	33.0	33.0	33.0	27.0	33.0
4	31.70264739964543	33.0	33.0	33.0	27.0	33.0
5	31.794118870522052	33.0	33.0	33.0	27.0	33.0
6	35.24123327423812	37.0	37.0	37.0	33.0	37.0
7	35.52609664543861	37.0	37.0	37.0	33.0	37.0
8	35.590666257291986	37.0	37.0	37.0	33.0	37.0
9	35.477369833708046	37.0	37.0	37.0	33.0	37.0
10	35.5500965662048	37.0	37.0	37.0	33.0	37.0
11	35.55467628036883	37.0	37.0	37.0	33.0	37.0
12	35.554103816098326	37.0	37.0	37.0	33.0	37.0
13	35.574872978299844	37.0	37.0	37.0	33.0	37.0
14	35.633082096130416	37.0	37.0	37.0	33.0	37.0
15	35.67970921192072	37.0	37.0	37.0	33.0	37.0
16	35.690978240415184	37.0	37.0	37.0	33.0	37.0
17	35.6982241722543	37.0	37.0	37.0	33.0	37.0
18	35.68868442164271	37.0	37.0	37.0	33.0	37.0
19	35.67533154396981	37.0	37.0	37.0	33.0	37.0
20	35.657973397248604	37.0	37.0	37.0	33.0	37.0
21	35.579228856953264	37.0	37.0	37.0	33.0	37.0
22	35.614299721691246	37.0	37.0	37.0	33.0	37.0
23	35.63797280299504	37.0	37.0	37.0	33.0	37.0
24	35.621319837174525	37.0	37.0	37.0	33.0	37.0
25	35.62903919102281	37.0	37.0	37.0	33.0	37.0
26	35.577384690047246	37.0	37.0	37.0	33.0	37.0
27	35.574587736587205	37.0	37.0	37.0	33.0	37.0
28	35.54792555983638	37.0	37.0	37.0	33.0	37.0
29	35.53718739786267	37.0	37.0	37.0	33.0	37.0
30	35.52638981053215	37.0	37.0	37.0	33.0	37.0
31	35.5094813156774	37.0	37.0	37.0	33.0	37.0
32	35.48722453871067	37.0	37.0	37.0	33.0	37.0
33	35.463598997692316	37.0	37.0	37.0	33.0	37.0
34	35.44157199877188	37.0	37.0	37.0	33.0	37.0
35	35.410048827834835	37.0	37.0	37.0	33.0	37.0
36	35.40543345845672	37.0	37.0	37.0	33.0	37.0
37	35.402279952855885	37.0	37.0	37.0	33.0	37.0
38	35.35825170600295	37.0	37.0	37.0	33.0	37.0
39	35.36560460348431	37.0	37.0	37.0	33.0	37.0
40	35.36419820337338	37.0	37.0	37.0	33.0	37.0
41	35.3448730773421	37.0	37.0	37.0	33.0	37.0
42	35.32666712886389	37.0	37.0	37.0	33.0	37.0
43	35.303505105628574	37.0	37.0	37.0	33.0	37.0
44	35.267752830132615	37.0	37.0	37.0	33.0	37.0
45	35.22758128893599	37.0	37.0	37.0	33.0	37.0
46	35.248435627482245	37.0	37.0	37.0	33.0	37.0
47	35.22686422296394	37.0	37.0	37.0	33.0	37.0
48	35.204981825745044	37.0	37.0	37.0	33.0	37.0
49	35.1970802341359	37.0	37.0	37.0	33.0	37.0
50	35.17680430239583	37.0	37.0	37.0	33.0	37.0
51	34.799643447859204	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	348.0
15	1471.0
16	1325.0
17	1041.0
18	935.0
19	964.0
20	987.0
21	1175.0
22	1320.0
23	1564.0
24	2043.0
25	2545.0
26	3327.0
27	4325.0
28	5742.0
29	6806.0
30	8083.0
31	10240.0
32	12639.0
33	17536.0
34	26723.0
35	56638.0
36	337058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.05129398714431	18.691849812314913	23.31118088088187	14.9456753196589
2	30.56959204492557	23.309794289223213	26.111105608763257	20.00950805708796
3	32.641358067487396	23.548288054512863	25.864292293521647	17.946061584478095
4	29.530242554498003	25.769211722642048	24.51771370843939	20.182832014420555
5	25.652936107837217	29.493398833282164	24.269117632493785	20.58454742638684
6	25.182683451028552	36.421999267087266	22.589360880287618	15.80595640159656
7	79.30016738142164	6.679806273336833	8.759297592282627	5.2607287529588875
8	78.78811889033051	5.756534313191438	8.73731021026672	6.718036586211336
9	73.77677855140789	7.230283161825151	11.86605524577337	7.126883040993592
10	40.254142442580246	24.738776035734446	18.313508374023197	16.693573147662107
11	31.31082432874107	25.00381312706132	22.571533273247695	21.113829270949914
12	27.634177503540762	22.944328344904772	29.502708805847455	19.918785345707015
13	24.43273544821575	25.131973813226104	30.404191468499608	20.031099270058533
14	19.638099577089545	28.406112888369467	28.71968068774946	23.236106846791525
15	18.96996048213773	25.558449790525618	34.534848019649985	20.93674170768667
16	20.129151108778114	24.95052839046421	31.724622896589977	23.195697604167698
17	21.049253716560855	23.740628126021374	28.106609090098743	27.103509067319028
18	21.965592718412946	24.843364663702	30.92396525597472	22.267077361910328
19	22.79358602315608	26.059603632870143	28.211395802588964	22.93541454138481
20	25.12642744659146	26.06851743639011	27.43034852971763	21.3747065873008
21	23.879089207364782	26.673665653134194	28.145433656541247	21.301811482959778
22	21.23208573098141	26.493804906553624	26.11902898966989	26.15508037279507
23	21.465033129636417	26.555409193102697	26.76914239305912	25.21041528420177
24	22.084047262967108	25.03530856616518	28.993433498073628	23.887210672794083
25	21.149880654075094	25.995226163003753	27.339625818336682	25.51526736458447
26	22.072360276129825	27.215624907147877	27.54266245406915	23.169352362653147
27	23.594045579248665	25.852209137639033	27.745897174324284	22.807848108788022
28	19.369100795309357	26.050689829350183	30.363980310398446	24.21622906494201
29	22.469123575029464	25.761090257212754	28.432854298929357	23.33693186882843
30	23.778858438895877	25.106420909802214	28.147810670813232	22.966909980488676
31	23.287410738161974	26.896708825655907	25.60381114621609	24.212069289966028
32	23.79014925668783	27.2189923440332	26.533223726564124	22.457634672714846
33	25.15336694167401	24.92457931799499	26.449037804431153	23.47301593589985
34	21.7734507314271	24.35667099151208	28.894589321263382	24.97528895579744
35	23.94465518436717	23.986054849604326	27.179771608545366	24.889518357483137
36	25.958580526310577	25.337189378707897	27.651608941535354	21.052621153446175
37	22.759317400734894	26.245010746085356	29.38108490893064	21.614586944249112
38	20.70696366139432	28.14622599463191	28.735131280517397	22.411679063456376
39	23.195895688690367	23.885031743044756	29.389602543405267	23.52947002485961
40	23.146572642546577	22.273019897590302	30.347143125971854	24.23326433389127
41	21.99728624203948	23.426862242118712	28.482771598641136	26.09307991720067
42	24.964592391573483	22.946903443699426	28.49029881050244	23.598205354224646
43	25.37918329751305	22.65987897035665	29.02928679667614	22.931650935454158
44	21.630631790585042	24.140164608238337	28.927273267503246	25.30193033367338
45	23.30761535947389	23.031683619400397	27.360424693216594	26.300276327909117
46	23.09130706072281	23.80857111729575	29.7968643220062	23.30325749997524
47	21.498311329444274	23.524914080838293	31.65152970772629	23.325244881991143
48	21.768102449315123	21.94637851971436	30.849683559975045	25.43583547099547
49	23.326235304604477	21.468994820089733	32.086721404023095	23.118048471282695
50	21.28101260807987	21.53753206493211	30.6638802777145	26.517575049273525
51	20.731526142204878	21.25902522606396	28.364118969564313	29.645329662166848
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	222.0
1	257.0
2	292.0
3	834.0
4	1376.0
5	1060.5
6	745.0
7	743.5
8	742.0
9	778.5
10	815.0
11	834.0
12	853.0
13	934.0
14	1015.0
15	1062.5
16	1110.0
17	1139.5
18	1169.0
19	1140.5
20	1112.0
21	1429.5
22	1747.0
23	1855.0
24	1963.0
25	2661.5
26	3600.5
27	3841.0
28	4813.5
29	5786.0
30	6167.5
31	6549.0
32	7471.5
33	8394.0
34	9066.5
35	9739.0
36	10883.0
37	12027.0
38	12885.5
39	13744.0
40	16176.0
41	18608.0
42	21398.0
43	24188.0
44	28352.5
45	32517.0
46	38088.0
47	43659.0
48	51187.0
49	58715.0
50	59632.0
51	60549.0
52	51626.5
53	42704.0
54	37150.0
55	31596.0
56	28290.0
57	24984.0
58	23071.5
59	21159.0
60	20017.5
61	18876.0
62	17271.5
63	15667.0
64	13652.0
65	11637.0
66	9926.0
67	8215.0
68	6676.5
69	5138.0
70	4384.0
71	3630.0
72	2973.5
73	2317.0
74	2043.5
75	1472.0
76	1174.0
77	963.0
78	752.0
79	432.5
80	113.0
81	142.0
82	171.0
83	123.5
84	76.0
85	47.0
86	18.0
87	9.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	504835.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.467601709207624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.30774135593299	20.811730572881366
2	12.70610427292904	7.742490485265262
3	5.295289111214061	4.8400427872672225
4	2.6303099731178192	3.2055694653084137
5	1.511501103988171	2.3025906809669605
6	0.9618242534457028	1.7582686960943787
7	0.7096001430915022	1.513387017275604
8	0.5571858793311381	1.3580893959564588
9	0.42622645038066814	1.168748795531479
>10	6.637681162352877	45.39750124466922
>50	0.18686780822331048	3.563640705771832
>100	0.06185519784395294	3.325191800832078
>500	0.005208858765806563	1.0644898701387024
>1k	0.0026044293829032817	1.9482584820410356
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	4078	0.80778868343122	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	2396	0.4746105163073083	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT	2146	0.42508938564085297	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	1201	0.2378995117216516	TruSeq Adapter, Index 13 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	791	0.1566848574286648	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	741	0.14678063129537375	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	728	0.14420553250071805	No Hit
ACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	682	0.13509364445809027	TruSeq Adapter, Index 16 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTG	661	0.13093386948210803	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC	643	0.12736834807412323	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTG	571	0.11310626244218408	No Hit
ACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTG	549	0.10874840294353601	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.96169045331643E-4	0.0	0.0	0.43598403438747313	0.0
2	3.96169045331643E-4	0.0	0.0	2.1339645626788952	0.0
3	3.96169045331643E-4	0.0	0.0	2.8561807323184802	0.0
4	3.96169045331643E-4	0.0	0.0	3.574435211504749	0.0
5	3.96169045331643E-4	0.0	0.0	5.351451464339834	0.0
6	3.96169045331643E-4	0.0	0.0	6.2343141818614	0.0
7	3.96169045331643E-4	0.0	0.0	7.210672794081234	0.0
8	3.96169045331643E-4	0.0	0.0	8.874978953519467	0.0
9	3.96169045331643E-4	0.0	0.0	9.507264749868769	0.0
10	3.96169045331643E-4	0.0	0.0	11.155327978448405	0.0
11	3.96169045331643E-4	0.0	0.0	13.736963562352056	0.0
12	3.96169045331643E-4	0.0	0.0	15.505660265235177	0.0
13	3.96169045331643E-4	0.0	0.0	16.1795438113443	0.0
14	3.96169045331643E-4	0.0	0.0	16.444778987193835	0.0
15	3.96169045331643E-4	0.0	0.0	16.974457000802243	0.0
16	3.96169045331643E-4	0.0	0.0	18.133845711965296	0.0
17	3.96169045331643E-4	0.0	0.0	19.47527409945824	0.0
18	7.92338090663286E-4	0.0	0.0	21.033605039270256	0.0
19	7.92338090663286E-4	0.0	0.0	21.88437806411996	0.0
20	7.92338090663286E-4	0.0	0.0	22.680677845236563	0.0
21	0.0013865916586607505	0.0	0.0	23.704180573850863	0.0
22	0.0021789297493240364	0.0	0.0	24.769082967702317	0.0
23	0.002377014271989858	0.0	0.0	25.818336684263176	0.0
24	0.0025750987946556794	0.0	0.0	26.671090554339536	0.0
25	0.002773183317321501	0.0	0.0	27.364782552715244	0.0
26	0.0033674368853189654	0.0	0.0	28.021432745352442	0.0
27	0.0033674368853189654	0.0	0.0	28.608159101488607	0.0
28	0.0033674368853189654	0.0	0.0	29.26778056196579	0.0
29	0.0033674368853189654	0.0	0.0	29.89986827379243	0.0
30	0.003565521407984787	0.0	0.0	30.6638802777145	0.0
31	0.003565521407984787	0.0	0.0	31.345489120207592	0.0
32	0.003565521407984787	0.0	0.0	32.038586865015304	0.0
33	0.003565521407984787	0.0	0.0	32.66275119593531	0.0
34	0.004159774975982251	0.0	0.0	33.300979527964586	0.0
35	0.004357859498648073	0.0	0.0	33.973080313369714	0.0
36	0.004357859498648073	0.0	0.0	34.638248140481544	0.0
37	0.004357859498648073	0.0	0.0	35.27449562728416	0.0
38	0.004555944021313894	0.0	0.0	35.90678142363346	0.0
39	0.004555944021313894	0.0	0.0	36.48954608931631	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	20	7.029524E-4	45.0	37
CTATGCG	25	3.8876868E-5	45.0	1
TCGTTCA	25	3.8876868E-5	45.0	16
TCCGCGC	20	7.029524E-4	45.0	13
GATCGTG	20	7.029524E-4	45.0	1
CTCAACG	20	7.029524E-4	45.0	1
TCCGATA	20	7.029524E-4	45.0	7
TTCACCG	35	1.2101191E-7	45.0	23
ACCGAAA	20	7.029524E-4	45.0	16
ATTCGGA	20	7.029524E-4	45.0	34
GCGCATT	20	7.029524E-4	45.0	7
TCTAGCG	35	1.2101191E-7	45.0	1
ATCCGAT	20	7.029524E-4	45.0	6
AAACGGA	20	7.029524E-4	45.0	23
TGCGCAC	20	7.029524E-4	45.0	23
CATAGCG	20	7.029524E-4	45.0	2
TGCGAAG	20	7.029524E-4	45.0	1
GCCCTAT	20	7.029524E-4	45.0	18
ACTACGG	25	3.8876868E-5	45.0	1
AATCTCC	20	7.029524E-4	45.0	44
>>END_MODULE