FastQCFastQC Report
Sat 18 Jun 2016
SRR3552789_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552789_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907420
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC104570.548227448595485No Hit
CCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC79860.4186807310398339No Hit
CTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGCT79250.4154826939006616No Hit
TCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC56030.2937475752587264No Hit
ACTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC32870.1723270176468738No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCC22940.12026716716821675No Hit
GCCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTG21200.11114489729582369No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG207.0344575E-445.02
TCGCATA207.0344575E-445.043
GATACCG207.0344575E-445.012
TAAACGT207.0344575E-445.036
TTATCCG207.0344575E-445.01
AGACGCG253.8917773E-545.01
TACGATA253.8917773E-545.012
CTCGCGA207.0344575E-445.013
TCTCCGA207.0344575E-445.013
CTCGACG406.8193913E-945.01
ATCGGCC207.0344575E-445.010
TTCGCGC253.8917773E-545.01
ATCGCCC207.0344575E-445.09
TCGCGAA207.0344575E-445.014
AAGTACG600.044.9999961
GTCGTAG650.041.5384641
CGTAAGG3900.040.9615362
TAGACCG501.0822987E-940.51
GTTCGCG501.0822987E-940.51
GCGTAAG2650.039.9056631