##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552789_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1907420
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6134102609808	31.0	31.0	34.0	30.0	34.0
2	31.779570309632906	31.0	31.0	34.0	30.0	34.0
3	31.830057879229535	31.0	31.0	34.0	30.0	34.0
4	35.4560899015424	37.0	35.0	37.0	33.0	37.0
5	35.34401547640268	37.0	35.0	37.0	33.0	37.0
6	35.32750993488587	37.0	35.0	37.0	33.0	37.0
7	35.81043084375754	37.0	35.0	37.0	35.0	37.0
8	35.82993100628074	37.0	35.0	37.0	35.0	37.0
9	37.420027052248585	39.0	37.0	39.0	35.0	39.0
10	36.70406989546088	39.0	37.0	39.0	32.0	39.0
11	36.538358096276646	38.0	35.0	39.0	32.0	39.0
12	36.543999748351176	39.0	35.0	39.0	32.0	39.0
13	36.57743077035996	39.0	35.0	39.0	32.0	39.0
14	37.6444112990322	39.0	36.0	41.0	33.0	41.0
15	37.70769730840612	39.0	36.0	41.0	33.0	41.0
16	37.74254700066058	39.0	36.0	41.0	33.0	41.0
17	37.75183913348921	39.0	36.0	41.0	33.0	41.0
18	37.70210493755963	39.0	36.0	41.0	33.0	41.0
19	37.692328905013056	39.0	36.0	41.0	33.0	41.0
20	37.65925490977341	39.0	36.0	41.0	32.0	41.0
21	37.63275261872057	39.0	36.0	41.0	32.0	41.0
22	37.59581843537344	39.0	36.0	41.0	32.0	41.0
23	37.52821507586164	39.0	36.0	41.0	32.0	41.0
24	37.52760430319489	39.0	36.0	41.0	32.0	41.0
25	37.50885961141228	39.0	36.0	41.0	32.0	41.0
26	37.42628681674723	39.0	36.0	41.0	32.0	41.0
27	37.39584884293968	39.0	36.0	41.0	32.0	41.0
28	37.35368350966227	39.0	36.0	41.0	32.0	41.0
29	37.351907812647454	39.0	36.0	41.0	32.0	41.0
30	37.27984869614453	39.0	36.0	41.0	32.0	41.0
31	37.192442671252266	39.0	36.0	41.0	31.0	41.0
32	37.10704249719517	39.0	36.0	40.0	31.0	41.0
33	37.04976145788552	39.0	36.0	40.0	31.0	41.0
34	36.99949775088864	39.0	35.0	40.0	31.0	41.0
35	36.99083107024148	39.0	35.0	40.0	31.0	41.0
36	36.94953497394386	39.0	35.0	40.0	31.0	41.0
37	36.90753321240209	39.0	35.0	40.0	31.0	41.0
38	36.88819085466232	39.0	35.0	40.0	31.0	41.0
39	36.84831552568391	39.0	35.0	40.0	31.0	41.0
40	36.76771974709293	39.0	35.0	40.0	30.0	41.0
41	36.70174004676474	39.0	35.0	40.0	30.0	41.0
42	36.65563483658554	39.0	35.0	40.0	30.0	41.0
43	36.609533296285036	39.0	35.0	40.0	30.0	41.0
44	36.511530234557675	39.0	35.0	40.0	30.0	41.0
45	36.469380629331766	39.0	35.0	40.0	30.0	41.0
46	36.354571620303865	38.0	35.0	40.0	30.0	41.0
47	36.27703756907236	38.0	35.0	40.0	30.0	41.0
48	36.250854557465054	38.0	35.0	40.0	30.0	41.0
49	36.22183525390318	38.0	35.0	40.0	30.0	41.0
50	36.128626102274275	38.0	35.0	40.0	29.0	41.0
51	34.638491260446045	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	12.0
11	8.0
12	10.0
13	9.0
14	30.0
15	40.0
16	87.0
17	206.0
18	390.0
19	667.0
20	1181.0
21	1834.0
22	2940.0
23	4240.0
24	6434.0
25	9095.0
26	12092.0
27	16295.0
28	21356.0
29	27894.0
30	36946.0
31	48528.0
32	64103.0
33	84224.0
34	122535.0
35	149358.0
36	177169.0
37	250733.0
38	405115.0
39	463826.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.53756382967569	20.631114280022228	27.27715972360571	9.554162166696376
2	30.284992293254763	23.56769877635759	27.426051944511435	18.72125698587621
3	31.165815604324166	24.530727369955226	27.183368109802768	17.120088915917837
4	27.54867831940527	27.585796520955007	26.43602352916505	18.42950163047467
5	24.30922397793879	30.899120277652536	25.517662601839135	19.273993142569545
6	25.958782019691522	35.39550806849042	24.107852491847627	14.537857419970432
7	83.46698681989284	4.76119575132902	7.206540772352183	4.565276656425957
8	84.13317465476928	3.6038208679787354	6.850038271591993	5.412966205660001
9	78.32590619790084	6.744555472837654	9.40972622705015	5.519812102211364
10	36.06526092837446	33.2536095878202	17.104465718090406	13.576663765714944
11	23.691845529563494	26.969047194639877	28.86427740088706	20.474829874909563
12	21.77401935598872	24.77016074068637	33.09197764519613	20.36384225812878
13	22.210420358389868	25.82970714368099	33.62012561470468	18.33974688322446
14	20.221765526208177	29.00609199861593	30.171802749263403	20.60033972591249
15	18.719422046534063	28.99932893646916	33.11845319856141	19.162795818435374
16	21.155906931876565	28.55684642081975	29.842614631282043	20.44463201602164
17	20.927063782491533	27.14289459059882	29.106069979343825	22.82397164756582
18	21.462551509368677	26.350095941114176	31.992324710865987	20.19502783865116
19	20.538371202986234	28.239978609849953	29.696134044940287	21.525516142223527
20	21.613383523293244	28.998437680217258	29.593167734426608	19.79501106206289
21	21.64368623585786	28.06602636021432	30.45548437155949	19.83480303236833
22	20.655912174560402	26.165605896970778	28.479516834257794	24.69896509421103
23	18.821444673957494	27.972392026926425	29.39551855385809	23.810644745257992
24	20.261295362321878	27.09932788793239	29.83915445995114	22.80022228979459
25	18.436684107328226	29.691939897872523	28.011607302010045	23.859768692789213
26	19.04053643140997	30.327825020184335	28.918539178576296	21.713099369829404
27	20.5020394040117	28.057847773432176	30.09101299137054	21.34909983118558
28	16.878086630107685	27.864235459416385	30.128393327112015	25.129284583363916
29	18.78133814262197	26.470363108282392	28.952459342986863	25.795839406108772
30	20.239223663377757	27.094190057774377	28.67265730672846	23.99392897211941
31	18.7565402480838	30.1241467532059	25.782732696522004	25.336580302188295
32	18.203070115653606	29.265709702110705	26.017447651801913	26.513772530433783
33	18.116880393410995	26.58696039676631	28.550188212349664	26.74597099747303
34	17.129473319981965	27.63224669973053	28.297333571001666	26.940946409285843
35	16.983359721508634	26.98241603841839	27.959337744177997	28.07488649589498
36	18.263203699237714	27.36298245797989	29.700013630977974	24.673800211804426
37	17.380126034119385	27.30536536263644	29.948149856874736	25.366358746369443
38	17.28937517694058	26.48299797632404	27.413469503308136	28.814157343427247
39	19.247203028174184	25.78572102630779	28.539021295781737	26.428054649736293
40	19.422256241415106	26.492854221933293	28.757798492204127	25.327091044447474
41	17.750311939688164	27.64587767770077	27.78381268939195	26.81999769321911
42	19.115454383407954	25.287456354657078	30.766847364502837	24.830241897432135
43	20.165249394470017	24.246783613467407	30.5803126736639	25.007654318398675
44	18.721571546906294	25.36153547724151	27.599112937895164	28.31778003795703
45	19.48228497132252	24.180096675089914	27.138910150884442	29.198708202703127
46	20.34428704742532	25.30522905285674	28.114573612523724	26.23591028719422
47	16.4449885185224	26.0670958677166	31.066466745656435	26.42144886810456
48	17.34458063772006	23.83371255413071	30.769206572228452	28.052500235920775
49	19.297742500340775	22.469199232471087	32.04669134223192	26.18636692495622
50	18.69299892000713	22.33902339285527	30.23183147917082	28.736146207966783
51	17.66794937664489	22.32319048767445	27.565874322383117	32.44298581329754
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	320.0
1	488.0
2	656.0
3	1185.5
4	1715.0
5	1379.5
6	1044.0
7	1161.5
8	1279.0
9	1414.0
10	1549.0
11	1699.0
12	1849.0
13	1996.0
14	2143.0
15	2354.0
16	2565.0
17	2635.0
18	2705.0
19	3037.0
20	3369.0
21	4084.5
22	4800.0
23	5507.5
24	6215.0
25	9864.0
26	14142.5
27	14772.0
28	19137.5
29	23503.0
30	27701.0
31	31899.0
32	38118.5
33	44338.0
34	50736.5
35	57135.0
36	62145.5
37	67156.0
38	74601.0
39	82046.0
40	92467.0
41	102888.0
42	115752.5
43	128617.0
44	141946.5
45	155276.0
46	179209.0
47	203142.0
48	228906.5
49	254671.0
50	243675.5
51	232680.0
52	192140.0
53	151600.0
54	125991.0
55	100382.0
56	84365.5
57	68349.0
58	58122.0
59	47895.0
60	42455.0
61	37015.0
62	31020.5
63	25026.0
64	20380.0
65	15734.0
66	12307.5
67	8881.0
68	6699.0
69	4517.0
70	3526.5
71	2536.0
72	1933.5
73	1331.0
74	1077.5
75	859.5
76	895.0
77	566.0
78	237.0
79	180.5
80	124.0
81	101.5
82	79.0
83	71.5
84	64.0
85	50.5
86	37.0
87	28.5
88	20.0
89	16.5
90	13.0
91	10.0
92	7.0
93	6.0
94	5.0
95	4.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1907420.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.948529497456374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.453536647294385	16.465259674102473
2	14.43859312018468	7.493205189617658
3	6.543238851890103	5.093622790715188
4	3.489975939803231	3.622389744775887
5	2.1336510390358527	2.7682553461850135
6	1.3773438151489914	2.144402796931962
7	0.9468826227680284	1.719914816726753
8	0.6787305110895757	1.4089646950265198
9	0.5286022352035896	1.2344805623341546
>10	5.332884670597676	33.58234607150456
>50	0.9717263948516652	15.964290507126758
>100	0.09428993909349936	4.57624157713568
>500	0.0077053864513539835	1.335569613419951
>1k	0.002027733276672101	0.908848202836655
>5k	6.083199830016303E-4	1.1319956137224454
>10k+	2.0277332766721013E-4	0.5502127978384127
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	10457	0.548227448595485	No Hit
CCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	7986	0.4186807310398339	No Hit
CTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGCT	7925	0.4154826939006616	No Hit
TCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	5603	0.2937475752587264	No Hit
ACTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	3287	0.1723270176468738	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCC	2294	0.12026716716821675	No Hit
GCCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTG	2120	0.11114489729582369	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4329408310702415	0.0
2	0.0	0.0	0.0	1.9353891644210504	0.0
3	0.0	0.0	0.0	2.6434660431368027	0.0
4	0.0	0.0	0.0	3.4646276121672206	0.0
5	0.0	0.0	0.0	5.4881462918497235	0.0
6	0.0	0.0	0.0	6.506485199903534	0.0
7	0.0	0.0	0.0	7.666795986201256	0.0
8	0.0	0.0	0.0	9.503622694529783	0.0
9	0.0	0.0	0.0	10.119271057239622	0.0
10	0.0	0.0	0.0	11.74051860628493	0.0
11	0.0	0.0	0.0	14.240806953895838	0.0
12	0.0	0.0	0.0	16.062692013295447	0.0
13	0.0	0.0	0.0	16.751895230206248	0.0
14	0.0	0.0	0.0	16.996833418963835	0.0
15	0.0	0.0	0.0	17.484088454561657	0.0
16	0.0	0.0	0.0	18.64298371622401	0.0
17	0.0	0.0	0.0	20.254060458629983	0.0
18	0.0	0.0	0.0	21.93014648058634	0.0
19	0.0	0.0	0.0	22.948852376508583	0.0
20	5.242683834708664E-5	0.0	0.0	23.885824831447714	0.0
21	4.194147067766931E-4	0.0	0.0	25.017929978714704	0.0
22	4.194147067766931E-4	0.0	0.0	26.29101089429701	0.0
23	5.242683834708664E-4	0.0	0.0	27.568548091138815	0.0
24	5.242683834708664E-4	0.0	0.0	28.54290088181942	0.0
25	0.0011009636052888194	0.0	0.0	29.36726048798901	0.0
26	0.001153390443635906	0.0	0.0	30.13374086462342	0.0
27	0.001153390443635906	0.0	0.0	30.882710677249896	0.0
28	0.0012058172819829928	0.0	0.0	31.680227742185778	0.0
29	0.0012582441203300794	0.0	0.0	32.484560296106785	0.0
30	0.0012582441203300794	0.0	0.0	33.390915477451216	0.0
31	0.0012582441203300794	0.0	0.0	34.238133185140136	0.0
32	0.0012582441203300794	0.0	0.0	35.016986295624456	0.0
33	0.001310670958677166	0.0	0.0	35.82262952050414	0.0
34	0.001467951473718426	0.0	0.0	36.549475207348145	0.0
35	0.0015728051504125993	0.0	0.0	37.33058267188139	0.0
36	0.0015728051504125993	0.0	0.0	38.08379905841398	0.0
37	0.0018349393421480324	0.0	0.0	38.82820773610427	0.0
38	0.0019397930188422058	0.0	0.0	39.578435792851074	0.0
39	0.001992219857189292	0.0	0.0	40.314141615375746	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGG	20	7.0344575E-4	45.0	2
TCGCATA	20	7.0344575E-4	45.0	43
GATACCG	20	7.0344575E-4	45.0	12
TAAACGT	20	7.0344575E-4	45.0	36
TTATCCG	20	7.0344575E-4	45.0	1
AGACGCG	25	3.8917773E-5	45.0	1
TACGATA	25	3.8917773E-5	45.0	12
CTCGCGA	20	7.0344575E-4	45.0	13
TCTCCGA	20	7.0344575E-4	45.0	13
CTCGACG	40	6.8193913E-9	45.0	1
ATCGGCC	20	7.0344575E-4	45.0	10
TTCGCGC	25	3.8917773E-5	45.0	1
ATCGCCC	20	7.0344575E-4	45.0	9
TCGCGAA	20	7.0344575E-4	45.0	14
AAGTACG	60	0.0	44.999996	1
GTCGTAG	65	0.0	41.538464	1
CGTAAGG	390	0.0	40.961536	2
TAGACCG	50	1.0822987E-9	40.5	1
GTTCGCG	50	1.0822987E-9	40.5	1
GCGTAAG	265	0.0	39.905663	1
>>END_MODULE