FastQCFastQC Report
Sat 18 Jun 2016
SRR3552788_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552788_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757270
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC46610.6155004159678846No Hit
GCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC35620.4703738428829876No Hit
CTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGCT34820.45980957914614334No Hit
TCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC17600.23241380221057217No Hit
ACTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC11250.14855995879937142No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCC9710.12822375110594636No Hit
GCCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTG8260.10907602308291628No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGCG351.2111923E-745.0000041
ACTGCGC207.0317596E-445.036
GTTTACG207.0317596E-445.01
TAAACGG406.8102963E-945.02
GTTCGCA207.0317596E-445.041
TAGTCGT207.0317596E-445.017
ACGACTA207.0317596E-445.027
TGCGTTG207.0317596E-445.01
TTATACG302.1645174E-644.9999961
CGCGCGG800.042.18752
GCGTAAG650.041.538461
CGGGCCA603.6379788E-1241.2499966
TTGGACG556.002665E-1140.9090921
TCAAGCG556.002665E-1140.90909217
CGTAAGG1050.040.7142832
TTTTGCG501.0804797E-940.51
CGTTTTT7600.039.3750041
CAGCGCG403.4561344E-739.3751
GCGCGCG800.039.3751
TCGATTG403.4561344E-739.3751