##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552788_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	757270
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.968672996421354	33.0	33.0	33.0	27.0	33.0
2	32.089773792702736	33.0	33.0	33.0	33.0	33.0
3	32.13841166294716	33.0	33.0	33.0	33.0	33.0
4	32.06870204814663	33.0	33.0	33.0	33.0	33.0
5	32.11341397387986	33.0	33.0	33.0	33.0	33.0
6	35.63266602400729	37.0	37.0	37.0	33.0	37.0
7	36.00604407939044	37.0	37.0	37.0	37.0	37.0
8	36.06185244364625	37.0	37.0	37.0	37.0	37.0
9	35.93713734863391	37.0	37.0	37.0	37.0	37.0
10	35.8639019108112	37.0	37.0	37.0	33.0	37.0
11	35.86211522970671	37.0	37.0	37.0	33.0	37.0
12	35.846490683639914	37.0	37.0	37.0	33.0	37.0
13	35.90165594834075	37.0	37.0	37.0	33.0	37.0
14	35.92866216805103	37.0	37.0	37.0	37.0	37.0
15	35.94998085227198	37.0	37.0	37.0	37.0	37.0
16	35.94280375559576	37.0	37.0	37.0	37.0	37.0
17	35.950270048991776	37.0	37.0	37.0	37.0	37.0
18	35.946079997887146	37.0	37.0	37.0	37.0	37.0
19	35.950926353876426	37.0	37.0	37.0	37.0	37.0
20	35.93815415901858	37.0	37.0	37.0	37.0	37.0
21	35.837591611974595	37.0	37.0	37.0	37.0	37.0
22	35.89946782521426	37.0	37.0	37.0	37.0	37.0
23	35.92512313969918	37.0	37.0	37.0	37.0	37.0
24	35.925113895968416	37.0	37.0	37.0	37.0	37.0
25	35.921391313534144	37.0	37.0	37.0	37.0	37.0
26	35.88742588508722	37.0	37.0	37.0	37.0	37.0
27	35.880507612872556	37.0	37.0	37.0	37.0	37.0
28	35.880065234328576	37.0	37.0	37.0	37.0	37.0
29	35.87135235781161	37.0	37.0	37.0	37.0	37.0
30	35.84539596181018	37.0	37.0	37.0	37.0	37.0
31	35.846239782376166	37.0	37.0	37.0	37.0	37.0
32	35.832539252842444	37.0	37.0	37.0	33.0	37.0
33	35.8175551652647	37.0	37.0	37.0	37.0	37.0
34	35.78887979188401	37.0	37.0	37.0	33.0	37.0
35	35.77456521452058	37.0	37.0	37.0	33.0	37.0
36	35.775250571130506	37.0	37.0	37.0	33.0	37.0
37	35.78118240521874	37.0	37.0	37.0	33.0	37.0
38	35.77060493615223	37.0	37.0	37.0	33.0	37.0
39	35.75760296855811	37.0	37.0	37.0	33.0	37.0
40	35.750931636008296	37.0	37.0	37.0	33.0	37.0
41	35.74500640458489	37.0	37.0	37.0	33.0	37.0
42	35.74147529943085	37.0	37.0	37.0	33.0	37.0
43	35.73170599654021	37.0	37.0	37.0	33.0	37.0
44	35.69242542290068	37.0	37.0	37.0	33.0	37.0
45	35.655108481783245	37.0	37.0	37.0	33.0	37.0
46	35.67971793415823	37.0	37.0	37.0	33.0	37.0
47	35.67421659381726	37.0	37.0	37.0	33.0	37.0
48	35.67137216580612	37.0	37.0	37.0	33.0	37.0
49	35.66029949687694	37.0	37.0	37.0	33.0	37.0
50	35.66736038665205	37.0	37.0	37.0	33.0	37.0
51	35.323259867682594	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	523.0
15	1847.0
16	1344.0
17	736.0
18	556.0
19	551.0
20	602.0
21	702.0
22	811.0
23	1123.0
24	1510.0
25	2153.0
26	3207.0
27	4594.0
28	6554.0
29	8240.0
30	10277.0
31	13170.0
32	17610.0
33	24528.0
34	38709.0
35	81447.0
36	536476.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57556749904261	19.589974513713734	25.684234156905728	12.150223830337925
2	29.167536017536676	23.481453114477002	27.38164723282317	19.969363635163152
3	31.364374661613425	24.510412402445628	26.436937948155876	17.688274987785068
4	28.47267157024575	27.168117052042206	25.774426558559032	18.58478481915301
5	24.802910454659504	30.510518045082996	25.200787037648393	19.48578446260911
6	25.938304699776833	35.669708294267565	23.4476474705191	14.944339535436502
7	82.90147503532425	5.213728260726029	7.233879593804058	4.650917110145655
8	84.04400015846396	3.987877507361971	6.5905159322302485	5.377606401943825
9	78.41364374661613	6.98548734269151	9.318076775786707	5.282792134905648
10	36.2582698377065	33.32998798315	16.850660926750034	13.561081252393468
11	23.820830087023122	27.266364704794853	28.80267275872542	20.1101324494566
12	22.13926340671095	24.77702800850423	32.890910771587414	20.19279781319741
13	22.352133321008356	25.739036275040604	33.38954401996646	18.519286383984575
14	20.513027057720496	28.586237405416824	30.209436528582934	20.691299008279742
15	18.985038361482694	28.61132753179183	32.9858570919223	19.417777014803175
16	21.363846448426585	28.119693108138442	29.98798314999934	20.528477293435632
17	21.269956554465384	26.867431695432277	29.273970974685383	22.588640775416955
18	21.750762607788506	25.754354457459026	32.35754750617349	20.137335428578975
19	20.554887952777744	27.911709165819325	30.157011369788844	21.376391511614088
20	21.899058459994453	28.373367491119417	29.91099607801709	19.81657797086904
21	21.998362539120787	27.906823193841035	30.396291943428366	19.69852232360981
22	20.896905991258073	26.238197736606494	28.500006602664836	24.3648896694706
23	18.96734321972348	28.044686835606853	29.23567551863932	23.752294426030346
24	20.13825980165595	27.033686795990864	30.062461209344093	22.7655921930091
25	18.363859653756258	29.51655288074267	28.277364744410843	23.84222272109023
26	18.92719901752347	30.20811599561583	29.283610865345256	21.581074121515446
27	20.242317799463862	27.899956422412085	30.428909107715874	21.428816670408178
28	17.143687192150754	27.81029223394562	30.34452705111783	24.701493522785796
29	18.973681777965588	26.263948129465053	29.233958825782086	25.528411266787277
30	20.31111756705006	27.22463586303432	28.682900418608952	23.781346151306668
31	19.022805604341915	30.070384407146722	26.20043049374728	24.706379494764086
32	18.554412560909583	29.10045294280772	26.319278460786773	26.025856035495927
33	18.392251112549022	26.639771811903284	28.935122215326103	26.032854860221587
34	17.45150342678305	27.469726781729104	28.55111122849182	26.527658562996027
35	17.14830905753562	27.078188756982318	28.1879646625378	27.585537522944257
36	18.394231911999682	27.299774188862624	29.720971384050603	24.58502251508709
37	17.573124513053468	27.465897236124498	29.758342467019688	25.20263578380234
38	17.32473226194092	26.540335679480236	27.599799278989	28.535132779589844
39	19.03653914719981	26.00618009428605	28.56576914442669	26.39151161408745
40	19.39070608897751	26.665786311355262	28.633248379045785	25.310259220621447
41	17.806330635044304	27.511983836676485	27.84106065207918	26.840624876200035
42	19.100452942807717	25.43901118491423	30.633855824210656	24.826680048067402
43	19.889603443949976	24.473437479366673	30.7245764390508	24.91238263763255
44	18.635757391683285	25.67195320031165	27.916198977907484	27.77609043009759
45	19.396648487329486	24.508299549698258	27.48927066964227	28.60578129332999
46	20.086891069235545	25.641316835474797	28.377064983427314	25.89472711186235
47	16.557898767942742	26.441559813540742	30.819522759385688	26.18101865913082
48	17.56810648777847	24.244721169463997	30.627121106078413	27.560051236679122
49	19.415268002165675	22.831618841364374	31.61105021986874	26.142062936601214
50	18.852720958178722	22.790418212790684	29.91997570219341	28.43688512683719
51	17.74386942570021	22.747104731469623	27.650903904815983	31.858121938014182
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	171.0
1	200.5
2	230.0
3	702.5
4	1175.0
5	941.5
6	708.0
7	741.5
8	775.0
9	786.0
10	797.0
11	915.0
12	1033.0
13	1025.0
14	1017.0
15	1091.5
16	1166.0
17	1185.0
18	1204.0
19	1344.0
20	1484.0
21	1755.0
22	2026.0
23	2287.5
24	2549.0
25	4033.5
26	5754.5
27	5991.0
28	7565.0
29	9139.0
30	10896.5
31	12654.0
32	14924.0
33	17194.0
34	19790.0
35	22386.0
36	24534.0
37	26682.0
38	29355.5
39	32029.0
40	36277.0
41	40525.0
42	45409.0
43	50293.0
44	54861.5
45	59430.0
46	68238.5
47	77047.0
48	87258.0
49	97469.0
50	95560.5
51	93652.0
52	77640.0
53	61628.0
54	51566.0
55	41504.0
56	34992.5
57	28481.0
58	24237.5
59	19994.0
60	17964.5
61	15935.0
62	13343.5
63	10752.0
64	8778.5
65	6805.0
66	5255.5
67	3706.0
68	2709.5
69	1713.0
70	1373.0
71	1033.0
72	795.0
73	557.0
74	446.5
75	324.0
76	312.0
77	189.5
78	67.0
79	55.0
80	43.0
81	35.0
82	27.0
83	23.5
84	20.0
85	16.0
86	12.0
87	6.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	757270.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.92979880415088
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.78340397884661	24.025904620457432
2	12.697034006948513	8.870096865443465
3	4.825989415327872	5.057125179250929
4	2.419100749875086	3.379948099204291
5	1.4650814609603937	2.5587500331518993
6	1.049272842304515	2.1990533563413748
7	0.8595862100572523	2.101762136048568
8	0.6838123583147011	1.9108342476579554
9	0.6245357361703644	1.9633416849389778
>10	6.453139161970538	40.602503980881664
>50	0.09104530272198155	2.172777782814149
>100	0.04380840712503253	2.6530002928393146
>500	0.002285656023914716	0.5635197231504877
>1k	0.0019047133532622638	1.941381997819508
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	4661	0.6155004159678846	No Hit
GCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	3562	0.4703738428829876	No Hit
CTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGCT	3482	0.45980957914614334	No Hit
TCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	1760	0.23241380221057217	No Hit
ACTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	1125	0.14855995879937142	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCC	971	0.12822375110594636	No Hit
GCCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTG	826	0.10907602308291628	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.47103410936654033	0.0
2	0.0	0.0	0.0	1.9724800929655208	0.0
3	0.0	0.0	0.0	2.5998653056373553	0.0
4	0.0	0.0	0.0	3.3017285776539413	0.0
5	0.0	0.0	0.0	4.959129504668084	0.0
6	0.0	0.0	0.0	5.813514334385358	0.0
7	0.0	0.0	0.0	6.8327016783974015	0.0
8	0.0	0.0	0.0	8.586633565306958	0.0
9	0.0	0.0	0.0	9.204775047209054	0.0
10	0.0	0.0	0.0	10.84619752532122	0.0
11	0.0	0.0	0.0	13.296050285895387	0.0
12	0.0	0.0	0.0	15.079694164564819	0.0
13	0.0	0.0	0.0	15.779576637130745	0.0
14	0.0	0.0	0.0	16.027704781649874	0.0
15	0.0	0.0	0.0	16.502964596511152	0.0
16	0.0	0.0	0.0	17.596894106461367	0.0
17	0.0	0.0	0.0	19.110224886764296	0.0
18	0.0	0.0	0.0	20.713351908830404	0.0
19	2.6410659342110474E-4	0.0	0.0	21.712995364929284	0.0
20	2.6410659342110474E-4	0.0	0.0	22.647800652343285	0.0
21	3.961598901316571E-4	0.0	0.0	23.746616134271793	0.0
22	7.923197802633142E-4	0.0	0.0	24.990162029395062	0.0
23	0.001056426373684419	0.0	0.0	26.224464193748595	0.0
24	0.0014525862638160763	0.0	0.0	27.18211470149352	0.0
25	0.0018487461539477333	0.0	0.0	27.986583385054207	0.0
26	0.0018487461539477333	0.0	0.0	28.793957241142525	0.0
27	0.0018487461539477333	0.0	0.0	29.50995021590714	0.0
28	0.0018487461539477333	0.0	0.0	30.293422425290846	0.0
29	0.0018487461539477333	0.0	0.0	31.079271594015346	0.0
30	0.0018487461539477333	0.0	0.0	31.94884255285433	0.0
31	0.0018487461539477333	0.0	0.0	32.772458964438044	0.0
32	0.0018487461539477333	0.0	0.0	33.560288932613204	0.0
33	0.0018487461539477333	0.0	0.0	34.33676231727125	0.0
34	0.0018487461539477333	0.0	0.0	35.05222707884902	0.0
35	0.0018487461539477333	0.0	0.0	35.8240785981222	0.0
36	0.0018487461539477333	0.0	0.0	36.553541009151296	0.0
37	0.002376959340789943	0.0	0.0	37.29356768391723	0.0
38	0.0025090126375004952	0.0	0.0	38.02012492241869	0.0
39	0.0025090126375004952	0.0	0.0	38.72423310047935	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGGCG	35	1.2111923E-7	45.000004	1
ACTGCGC	20	7.0317596E-4	45.0	36
GTTTACG	20	7.0317596E-4	45.0	1
TAAACGG	40	6.8102963E-9	45.0	2
GTTCGCA	20	7.0317596E-4	45.0	41
TAGTCGT	20	7.0317596E-4	45.0	17
ACGACTA	20	7.0317596E-4	45.0	27
TGCGTTG	20	7.0317596E-4	45.0	1
TTATACG	30	2.1645174E-6	44.999996	1
CGCGCGG	80	0.0	42.1875	2
GCGTAAG	65	0.0	41.53846	1
CGGGCCA	60	3.6379788E-12	41.249996	6
TTGGACG	55	6.002665E-11	40.909092	1
TCAAGCG	55	6.002665E-11	40.909092	17
CGTAAGG	105	0.0	40.714283	2
TTTTGCG	50	1.0804797E-9	40.5	1
CGTTTTT	760	0.0	39.375004	1
CAGCGCG	40	3.4561344E-7	39.375	1
GCGCGCG	80	0.0	39.375	1
TCGATTG	40	3.4561344E-7	39.375	1
>>END_MODULE