##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552785_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	651431
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.597957419895582	31.0	31.0	34.0	30.0	34.0
2	31.77456092817198	31.0	31.0	34.0	30.0	34.0
3	31.82158970021384	31.0	31.0	34.0	30.0	34.0
4	35.43049071966179	37.0	35.0	37.0	33.0	37.0
5	35.32513036683854	37.0	35.0	37.0	33.0	37.0
6	35.31848653195811	37.0	35.0	37.0	33.0	37.0
7	35.79582488398618	37.0	35.0	37.0	35.0	37.0
8	35.821889041203136	37.0	35.0	37.0	35.0	37.0
9	37.41880414042316	39.0	37.0	39.0	35.0	39.0
10	36.73246897983056	39.0	37.0	39.0	32.0	39.0
11	36.5292057025226	38.0	35.0	39.0	32.0	39.0
12	36.477594710721476	38.0	35.0	39.0	32.0	39.0
13	36.43460320433016	38.0	35.0	39.0	32.0	39.0
14	37.46237897797311	39.0	36.0	41.0	32.0	41.0
15	37.57468250666609	39.0	36.0	41.0	32.0	41.0
16	37.65239910289808	39.0	36.0	41.0	33.0	41.0
17	37.67568783186554	39.0	36.0	41.0	33.0	41.0
18	37.60649861612358	39.0	36.0	41.0	33.0	41.0
19	37.59166511879232	39.0	36.0	41.0	32.0	41.0
20	37.567662576696534	39.0	36.0	41.0	32.0	41.0
21	37.49177579820426	39.0	36.0	41.0	32.0	41.0
22	37.46393708619946	39.0	36.0	41.0	32.0	41.0
23	37.41812563418075	39.0	36.0	41.0	32.0	41.0
24	37.39442243307426	39.0	36.0	41.0	32.0	41.0
25	37.3654569708841	39.0	36.0	41.0	32.0	41.0
26	37.27483494030834	39.0	35.0	41.0	32.0	41.0
27	37.24341334692392	39.0	35.0	40.0	32.0	41.0
28	37.19102867379661	39.0	35.0	40.0	32.0	41.0
29	37.21153122894059	39.0	36.0	41.0	32.0	41.0
30	37.13870079870316	39.0	36.0	40.0	31.0	41.0
31	37.0243786371849	39.0	35.0	40.0	31.0	41.0
32	36.894946663576036	39.0	35.0	40.0	31.0	41.0
33	36.76734911295287	39.0	35.0	40.0	31.0	41.0
34	36.714394924404886	39.0	35.0	40.0	30.0	41.0
35	36.7097773977597	39.0	35.0	40.0	30.0	41.0
36	36.61721962878647	39.0	35.0	40.0	30.0	41.0
37	36.57360027385863	39.0	35.0	40.0	30.0	41.0
38	36.574430753218685	39.0	35.0	40.0	30.0	41.0
39	36.541403464066036	39.0	35.0	40.0	30.0	41.0
40	36.486990947621464	39.0	35.0	40.0	30.0	41.0
41	36.40031100761247	39.0	35.0	40.0	30.0	41.0
42	36.32620185407203	38.0	35.0	40.0	30.0	41.0
43	36.25804421343166	38.0	35.0	40.0	30.0	41.0
44	36.17359935280943	38.0	35.0	40.0	30.0	41.0
45	36.17226843671855	38.0	35.0	40.0	30.0	41.0
46	36.08110452219805	38.0	35.0	40.0	30.0	41.0
47	35.97239462045865	38.0	35.0	40.0	29.0	41.0
48	35.93984934705287	38.0	35.0	40.0	29.0	41.0
49	35.910787174696935	38.0	35.0	40.0	29.0	41.0
50	35.810348294754164	38.0	34.0	40.0	28.0	41.0
51	34.316871625697885	37.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	1.0
13	3.0
14	8.0
15	14.0
16	37.0
17	104.0
18	183.0
19	337.0
20	577.0
21	795.0
22	1286.0
23	1864.0
24	2687.0
25	3744.0
26	4813.0
27	6136.0
28	7680.0
29	10156.0
30	13196.0
31	17199.0
32	22383.0
33	29885.0
34	46046.0
35	55673.0
36	59447.0
37	83082.0
38	133299.0
39	150772.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.038929679428826	22.037944156787134	27.945400203551873	9.977725960232165
2	29.938397159484275	24.595697779196875	26.826785952771665	18.639119108547185
3	30.335215855554925	23.95234491450361	28.28726296415123	17.42517626579024
4	27.384481242065544	27.108319990912317	27.511279014968586	17.995919752053556
5	25.089380149240668	30.43300057872591	25.45779982837783	19.019819443655585
6	24.897648407889708	35.535152610176674	23.717477369053668	15.849721612879952
7	81.42059558111296	5.497589153724646	8.047206841553441	5.0346084236089474
8	82.46567940426537	3.8748539753250917	7.562734963488074	6.096731656921454
9	76.74151214787138	7.149490890055893	10.297790556482575	5.811206405590155
10	37.65448681441319	34.07452209059747	16.45193428006957	11.819056814919769
11	24.80907417669715	25.15615621608428	30.848393766953063	19.18637584026551
12	23.27967198367901	22.374894654997995	34.97162400929646	19.373809352026537
13	21.991124155896788	25.458874385775314	34.58155967400999	17.96844178431791
14	18.138221853120285	29.636753547190725	30.837187668379308	21.387836931309685
15	16.472350870621753	27.926825711395374	35.94333091302072	19.65749250496215
16	18.83177189909599	26.408936633350272	33.26522686209284	21.4940646054609
17	19.18683636486443	26.328037812139737	29.094409077860895	25.390716745134938
18	19.198042463438185	26.42167781392043	33.18095086049021	21.19932886215117
19	19.9075573621765	27.399832062029596	30.258461755734682	22.434148820059225
20	21.651871034691318	27.91316348162737	30.137957819016904	20.297007664664406
21	20.5286515379219	27.61397600052807	31.956722968357354	19.90064949319268
22	18.35635700480941	26.69415486828229	29.147215898537223	25.802272228371077
23	17.474605906074473	27.194591599110268	30.45249612008025	24.878306374735008
24	20.239135073399947	25.882403508583412	30.75367920777488	23.124782210241758
25	16.90892819039929	28.727985005319056	29.32482488552126	25.03826191876039
26	17.444057774346017	31.010805442172696	29.36627209942419	22.178864684057096
27	18.70789078198612	29.526534659848856	29.957432176239696	21.80814238192533
28	15.740884299334848	29.779823189255655	30.741859076402566	23.73743343500693
29	17.147479932640604	26.835228903751897	31.36218571114976	24.655105452457743
30	19.311792039371785	27.6546556734328	28.157702043654666	24.87585024354076
31	19.491089616551868	29.624165874820203	25.69450947222346	25.190235036404466
32	20.743870033817856	28.898379106919997	26.948824971485852	23.408925887776295
33	19.458545878228087	27.133648843852992	27.473055473258107	25.934749804660818
34	17.68337705758553	27.330292847592453	28.828072351484657	26.15825774333736
35	19.128656757200684	26.240998662943582	27.943711613355827	26.686632966499907
36	19.50122115772814	27.09957002353281	28.330552276449843	25.068656542289208
37	17.935437521395205	28.08401810782723	30.48457933380512	23.49596503697245
38	18.53089582780064	27.277946551515047	29.983221553779295	24.207936066905013
39	19.41249341833594	24.383856463693007	31.65293024126884	24.550719876702214
40	21.110601122758972	23.22717217940196	30.102036900301027	25.560189797538037
41	19.3929978769816	25.744399637106618	27.414722357394723	27.447880128517067
42	20.933913184972774	25.236901529095178	29.037918060393196	24.791267225538853
43	21.20239902614398	24.921288670634343	29.991510996559885	23.884801306661796
44	18.427891825841876	26.18512167827444	28.582766248459162	26.804220247424514
45	19.566001617976426	24.55563213909071	27.77869029874231	28.09967594419056
46	21.073145122046693	24.658329124650194	28.812414515121326	25.45611123818179
47	17.155001834422986	25.391944810732063	31.957644017555197	25.495409337289754
48	18.47854953172324	23.361184837688107	31.534575419346023	26.62569021124263
49	20.117710087484323	21.670752543247097	33.34059938811632	24.870937981152263
50	18.861091965227324	22.270662587442107	31.091090230584665	27.777155216745903
51	18.687474191433935	21.568976606885457	27.975180794282128	31.76836840739848
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	325.0
1	404.5
2	484.0
3	791.5
4	1099.0
5	841.5
6	584.0
7	636.5
8	689.0
9	803.5
10	918.0
11	922.0
12	926.0
13	993.0
14	1060.0
15	1091.0
16	1122.0
17	1346.5
18	1571.0
19	1697.5
20	1824.0
21	1974.0
22	2124.0
23	2531.0
24	2938.0
25	3318.0
26	4741.5
27	5785.0
28	8079.0
29	10373.0
30	11327.5
31	12282.0
32	14440.0
33	16598.0
34	17850.0
35	19102.0
36	20612.0
37	22122.0
38	24814.5
39	27507.0
40	30511.0
41	33515.0
42	37985.5
43	42456.0
44	48820.5
45	55185.0
46	63301.0
47	71417.0
48	80259.5
49	89102.0
50	83398.5
51	77695.0
52	62494.5
53	47294.0
54	39039.5
55	30785.0
56	25595.5
57	20406.0
58	18283.5
59	16161.0
60	14736.5
61	13312.0
62	11146.0
63	8980.0
64	7140.0
65	5300.0
66	4243.0
67	3186.0
68	2360.0
69	1534.0
70	1223.0
71	912.0
72	747.5
73	583.0
74	391.5
75	160.5
76	121.0
77	85.5
78	50.0
79	58.5
80	67.0
81	37.5
82	8.0
83	10.0
84	12.0
85	10.5
86	9.0
87	6.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	2.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	651431.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.45052610589505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.0034440628859	17.193752941584524
2	13.886355614921001	7.346028234164205
3	6.270066678057494	4.975396870609872
4	3.347010506835872	3.5412075515106904
5	2.0438871073676226	2.703094464546481
6	1.3110774306226174	2.0807212683320007
7	0.867978511194143	1.607094178878762
8	0.6256123478110377	1.3238220590356917
9	0.503193782103616	1.1978766242870185
>10	5.008298779157373	32.13430175721502
>50	1.0236105677071776	17.629268798366937
>100	0.10073073277209338	4.921866130780713
>500	0.0058225857093695595	1.0292518728333282
>1k	0.0029112928546847798	2.3163172478547445
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC	4545	0.6976947673659989	No Hit
CTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGCT	3316	0.5090331900078442	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC	3037	0.4662044023081493	No Hit
TCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC	2477	0.38023981050947836	No Hit
ACTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC	1665	0.2555911524014055	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCC	866	0.13293810088865898	No Hit
GCCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTG	832	0.12771882210088253	No Hit
TGCATGGGGACTCACTGTGGTGGTTTTATCCTCTCGTTTTTCTCCCTTGGT	817	0.12541619910627527	No Hit
AGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTG	718	0.11021888734186736	No Hit
ACCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTG	713	0.10945134634366493	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5350819964048379E-4	0.0	0.0	0.5320594199539168	0.0
2	1.5350819964048379E-4	0.0	0.0	2.431723390504904	0.0
3	1.5350819964048379E-4	0.0	0.0	3.2800097017182175	0.0
4	1.5350819964048379E-4	0.0	0.0	4.234523687082746	0.0
5	1.5350819964048379E-4	0.0	0.0	6.473594287038842	0.0
6	1.5350819964048379E-4	0.0	0.0	7.5493797501193525	0.0
7	1.5350819964048379E-4	0.0	0.0	8.76654626506875	0.0
8	1.5350819964048379E-4	0.0	0.0	10.755859024209778	0.0
9	1.5350819964048379E-4	0.0	0.0	11.426229332039771	0.0
10	1.5350819964048379E-4	0.0	0.0	13.052495199031057	0.0
11	1.5350819964048379E-4	0.0	0.0	15.78877885762268	0.0
12	1.5350819964048379E-4	0.0	0.0	17.81953883066664	0.0
13	1.5350819964048379E-4	0.0	0.0	18.547474713361815	0.0
14	1.5350819964048379E-4	0.0	0.0	18.814885997135537	0.0
15	1.5350819964048379E-4	0.0	0.0	19.306419252384366	0.0
16	1.5350819964048379E-4	0.0	0.0	20.479989438635865	0.0
17	1.5350819964048379E-4	0.0	0.0	22.127439437177536	0.0
18	1.5350819964048379E-4	0.0	0.0	23.886489896857842	0.0
19	1.5350819964048379E-4	0.0	0.0	24.96519201573152	0.0
20	1.5350819964048379E-4	0.0	0.0	25.911416558315462	0.0
21	4.605245989214514E-4	0.0	0.0	27.045995661858278	0.0
22	9.210491978429028E-4	0.0	0.0	28.262701652208754	0.0
23	9.210491978429028E-4	0.0	0.0	29.488618134537656	0.0
24	0.0013815737967643542	0.0	0.0	30.361619265893086	0.0
25	0.00322367219245016	0.0	0.0	31.10198931275914	0.0
26	0.0035306885917311273	0.0	0.0	31.753478112033353	0.0
27	0.0035306885917311273	0.0	0.0	32.43336592824106	0.0
28	0.0036841967913716113	0.0	0.0	33.12215722002791	0.0
29	0.0036841967913716113	0.0	0.0	33.853777299514455	0.0
30	0.0036841967913716113	0.0	0.0	34.71495829949757	0.0
31	0.003837704991012095	0.0	0.0	35.488793133885245	0.0
32	0.003837704991012095	0.0	0.0	36.23898770552829	0.0
33	0.003837704991012095	0.0	0.0	36.93315178430256	0.0
34	0.004144721390293062	0.0	0.0	37.60367560033219	0.0
35	0.004144721390293062	0.0	0.0	38.28555902313522	0.0
36	0.00445173778957403	0.0	0.0	38.9456442815893	0.0
37	0.004605245989214514	0.0	0.0	39.592988359473225	0.0
38	0.004605245989214514	0.0	0.0	40.230814928979434	0.0
39	0.004758754188854998	0.0	0.0	40.87201867887773	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACGG	65	0.0	45.000004	2
TATTGCG	65	0.0	45.000004	1
TCCGGCA	20	7.0310343E-4	45.0	17
TTTAGCG	25	3.8889382E-5	45.0	1
GTCGCTT	20	7.0310343E-4	45.0	27
GTCGCGC	35	1.2108467E-7	45.0	9
CGAACGA	20	7.0310343E-4	45.0	16
ACACGCC	20	7.0310343E-4	45.0	32
GCGAATG	35	1.2108467E-7	45.0	1
CCCTATC	20	7.0310343E-4	45.0	20
GCGAAAG	50	2.1827873E-11	45.0	1
GCGCGAC	55	1.8189894E-12	45.0	9
CGTGAGT	20	7.0310343E-4	45.0	33
CGTTGCC	25	3.8889382E-5	45.0	15
CACGCCA	50	2.1827873E-11	45.0	27
CATCTAG	20	7.0310343E-4	45.0	20
ATTCGGC	25	3.8889382E-5	45.0	17
CGCGAGA	35	1.2108467E-7	45.0	41
CGCGACC	55	1.8189894E-12	45.0	10
CGCGAAG	20	7.0310343E-4	45.0	2
>>END_MODULE