FastQCFastQC Report
Sat 18 Jun 2016
SRR3552782_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552782_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906252
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC77970.8603567219713723No Hit
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC72480.7997775453185206No Hit
CTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGCT71820.7924948027700905Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC33260.36700608660725714No Hit
ACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC20420.22532364066506888No Hit
GCCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTG15340.16926859195896948No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCC14020.15470310686210897No Hit
ACCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTG12940.14278589178285953No Hit
CGCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTG11470.12656523792499216No Hit
GGCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTG10090.11133768532372894No Hit
GGCCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCT9680.10681355737697683No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTC351.2115561E-745.023
GACGTAA253.8901497E-545.016
TAGCGCG502.1827873E-1145.01
GATCCGC351.2115561E-745.09
GCACGCG453.8380676E-1045.01
TTCCGGT207.0324965E-445.024
CGATTCA253.8901497E-545.010
CGATTAT207.0324965E-445.010
CTGCGAC207.0324965E-445.029
GAGACGT253.8901497E-545.014
TGCGACA207.0324965E-445.030
CGAATCG253.8901497E-545.02
TCCGTAG253.8901497E-545.01
CTCGCAG207.0324965E-445.01
ACGTAAG502.1827873E-1145.01
GCGTGAT207.0324965E-445.013
ATACGCG253.8901497E-545.01
TATCGCG406.8121153E-945.01
TGATCCG302.164992E-644.9999961
GTGTCGA302.164992E-644.9999969
Produced by FastQC (version 0.11.5)