##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552782_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	906252
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.098176886782042	33.0	33.0	33.0	27.0	33.0
2	31.308906352758395	33.0	33.0	33.0	27.0	33.0
3	31.428950225765018	33.0	33.0	33.0	27.0	33.0
4	31.366707052784434	33.0	33.0	33.0	27.0	33.0
5	31.481320868809117	33.0	33.0	33.0	27.0	33.0
6	34.85489797539757	37.0	37.0	37.0	27.0	37.0
7	35.35193853365289	37.0	37.0	37.0	33.0	37.0
8	35.46150298151066	37.0	37.0	37.0	33.0	37.0
9	35.35518707820783	37.0	37.0	37.0	33.0	37.0
10	35.29403410971783	37.0	37.0	37.0	33.0	37.0
11	35.29386417905836	37.0	37.0	37.0	33.0	37.0
12	35.303597674818924	37.0	37.0	37.0	33.0	37.0
13	35.369418219214964	37.0	37.0	37.0	33.0	37.0
14	35.41999355587629	37.0	37.0	37.0	33.0	37.0
15	35.44079792375631	37.0	37.0	37.0	33.0	37.0
16	35.45473554816982	37.0	37.0	37.0	33.0	37.0
17	35.46483649139533	37.0	37.0	37.0	33.0	37.0
18	35.468644482991486	37.0	37.0	37.0	33.0	37.0
19	35.45488120302079	37.0	37.0	37.0	33.0	37.0
20	35.45001611030927	37.0	37.0	37.0	33.0	37.0
21	35.395082162577296	37.0	37.0	37.0	33.0	37.0
22	35.41020930160706	37.0	37.0	37.0	33.0	37.0
23	35.42873064004272	37.0	37.0	37.0	33.0	37.0
24	35.419607349832056	37.0	37.0	37.0	33.0	37.0
25	35.4041657287377	37.0	37.0	37.0	33.0	37.0
26	35.36871973799782	37.0	37.0	37.0	33.0	37.0
27	35.35228280875518	37.0	37.0	37.0	33.0	37.0
28	35.326708244505944	37.0	37.0	37.0	33.0	37.0
29	35.341712901047394	37.0	37.0	37.0	33.0	37.0
30	35.26028190834337	37.0	37.0	37.0	33.0	37.0
31	35.22962928633537	37.0	37.0	37.0	33.0	37.0
32	35.210123674209825	37.0	37.0	37.0	33.0	37.0
33	35.18090111801133	37.0	37.0	37.0	33.0	37.0
34	35.15523496775731	37.0	37.0	37.0	33.0	37.0
35	35.13244329391825	37.0	37.0	37.0	33.0	37.0
36	35.11152527111664	37.0	37.0	37.0	33.0	37.0
37	35.11679201811417	37.0	37.0	37.0	33.0	37.0
38	35.09331510440805	37.0	37.0	37.0	33.0	37.0
39	35.09811950759833	37.0	37.0	37.0	33.0	37.0
40	35.06912536468885	37.0	37.0	37.0	33.0	37.0
41	35.04188901100356	37.0	37.0	37.0	33.0	37.0
42	35.04466748763037	37.0	37.0	37.0	33.0	37.0
43	35.0369577115416	37.0	37.0	37.0	33.0	37.0
44	35.00208440919303	37.0	37.0	37.0	27.0	37.0
45	34.78904212073463	37.0	37.0	37.0	27.0	37.0
46	34.90666944735018	37.0	37.0	37.0	27.0	37.0
47	34.89322395978161	37.0	37.0	37.0	27.0	37.0
48	34.906729033425584	37.0	37.0	37.0	27.0	37.0
49	34.91405922414516	37.0	37.0	37.0	27.0	37.0
50	34.890983964725045	37.0	37.0	37.0	27.0	37.0
51	34.447935011453765	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	554.0
15	2453.0
16	2641.0
17	2235.0
18	2190.0
19	2193.0
20	2214.0
21	2483.0
22	2951.0
23	3511.0
24	4344.0
25	5537.0
26	7525.0
27	9945.0
28	12897.0
29	14840.0
30	17758.0
31	21215.0
32	26770.0
33	36260.0
34	55291.0
35	113166.0
36	557278.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.44530660346129	17.910581162855365	23.94543681007049	12.69867542361286
2	31.37228938529239	22.891977065981646	25.97511508940118	19.760618459324782
3	31.512978729977974	23.870733526657045	26.29301783609857	18.32326990726641
4	28.113482784037995	26.65527910559094	26.583996504283576	18.64724160608749
5	24.100139916932598	29.81334110159205	26.12860440583855	19.9579145756368
6	25.049655062830205	35.4775492909257	24.41473232610797	15.05806332013612
7	79.18868041118806	5.869338771114436	9.246655455656926	5.69532536204058
8	79.00892908374271	4.812679034087649	9.051786920194383	7.126604961975256
9	74.00524357463487	7.2335288639363	12.073242321120395	6.687985240308435
10	36.2143200787419	28.547026654837726	20.4956237337959	14.74302953262448
11	25.0802205126168	26.316190198752665	28.51513707004233	20.088452218588206
12	22.433053940846477	23.792499216553452	34.06657309445938	19.707873748140695
13	22.721273994429804	26.84021662848744	33.62894647404916	16.809562903033594
14	20.953664102258532	28.985756721088617	29.886278871660423	20.17430030499243
15	18.943737503475855	27.48529106694385	34.49658593856896	19.07438549101133
16	20.22450709074297	27.40286366264571	30.77510449632111	21.597524750290205
17	20.897057330632098	26.908519926024994	28.752488270370712	23.441934472972196
18	21.30996676421128	24.78659357441418	33.729801423886514	20.173638237488028
19	20.91272626156963	27.267912236331615	30.88258012120249	20.936781380896264
20	22.580253615992017	27.667469975238674	30.493174084029604	19.259102324739697
21	21.383456257199985	28.697867701257486	31.37615144573474	18.542524595807787
22	19.209888640245758	26.73715478696875	28.60716445315431	25.445792119631182
23	19.086964773594982	27.443470469582408	29.610527756076678	23.859037000745932
24	20.72966459660227	25.795805140292106	29.935602900738427	23.5389273623672
25	18.144622025661736	27.60501494065668	28.221399787255642	26.02896324642594
26	17.558692284265305	28.965122283868062	29.51055556291186	23.96562986895477
27	20.103459082021335	29.200707970851376	28.9186672139758	21.777165733151485
28	18.602662394124373	26.609706792371217	30.867793946937493	23.919836866566914
29	18.844979100735777	25.510674735062654	31.117172706929196	24.52717345727237
30	20.82290577013899	29.769865335469603	27.380353367496017	22.026875526895388
31	20.763761073079014	28.499137105352595	25.89423250927998	24.842869312288414
32	20.924533132064813	27.975883087706286	27.409925716026006	23.68965806420289
33	20.472230681973667	28.032820893084924	27.886614319195985	23.60833410574542
34	19.61750153378972	24.8630623711727	28.696102187912416	26.823333907125168
35	19.49700524798842	25.876356686661104	28.126834478710116	26.499803586640358
36	22.27404739520575	27.271332918437697	28.05455877614615	22.400060910210403
37	20.96833993193946	27.066754059577246	30.341339936353247	21.62356607213005
38	21.542021424504444	28.375882204949615	28.624267863684715	21.457828506861226
39	20.53810639866174	25.71723979643631	31.15082780506967	22.593825999832276
40	21.917634388668937	24.327008381774608	28.304599603642256	25.450757625914207
41	20.344561998208004	24.82797279343935	26.703941067164543	28.1235241411881
42	22.270406023931532	23.431341392901754	29.505038333708505	24.79321424945821
43	22.328005896814574	24.128167441285647	30.40953288930673	23.134293772593054
44	20.321941358474245	24.679780017037203	29.169039075224113	25.829239549264443
45	20.551458093333864	24.36066347991508	28.303054779465313	26.784823647285744
46	22.50555033257858	24.92209672364861	28.7246814351858	23.847671508587016
47	18.775572357357557	24.282980892731825	32.320921774517466	24.620524975393156
48	20.567789091775797	23.007507845499926	31.301337817737227	25.123365244987045
49	22.0865719468757	20.722271509469774	33.72207730300181	23.46907924065271
50	20.693030194691982	21.113222370819596	30.829614720850273	27.36413271363815
51	19.908921580310995	21.03984322241496	27.67364927194643	31.37758592532761
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	786.0
1	707.5
2	629.0
3	1225.0
4	1821.0
5	1572.0
6	1323.0
7	1338.5
8	1354.0
9	1459.0
10	1564.0
11	1664.0
12	1764.0
13	1771.0
14	1778.0
15	1871.5
16	1965.0
17	1942.5
18	1920.0
19	2038.0
20	2156.0
21	2379.5
22	2603.0
23	3173.5
24	3744.0
25	4196.5
26	5804.5
27	6960.0
28	8065.0
29	9170.0
30	11359.0
31	13548.0
32	15603.5
33	17659.0
34	20205.0
35	22751.0
36	24515.0
37	26279.0
38	29205.5
39	32132.0
40	35820.0
41	39508.0
42	47876.5
43	56245.0
44	61444.5
45	66644.0
46	79077.0
47	91510.0
48	110341.0
49	129172.0
50	129402.0
51	129632.0
52	106716.0
53	83800.0
54	68742.0
55	53684.0
56	43843.0
57	34002.0
58	28358.0
59	22714.0
60	19564.0
61	16414.0
62	13473.5
63	10533.0
64	8729.5
65	6926.0
66	5531.0
67	4136.0
68	3105.0
69	2074.0
70	1615.0
71	1156.0
72	890.5
73	625.0
74	445.5
75	262.0
76	258.0
77	219.5
78	181.0
79	172.5
80	164.0
81	95.0
82	26.0
83	22.0
84	18.0
85	12.5
86	7.0
87	4.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	906252.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.205395696297256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.19313642630655	19.993898781355774
2	13.304460489832184	8.037329872422678
3	6.205920838233022	5.623568837361756
4	3.3799739438269665	4.0837380166587165
5	2.020231840791124	3.0510951074677455
6	1.3186580204938725	2.3898352378268086
7	0.9079560087914952	1.9197619364264507
8	0.6543753622796336	1.5812533401251363
9	0.47790060469201	1.2991659181399706
>10	5.034819949529419	35.44023023433758
>50	0.42516395478063146	7.966180828589333
>100	0.06859847773871847	3.8210932087999416
>500	0.005135714910919545	1.0276009400727424
>1k	0.0025678574554597725	1.3023961019640948
>5k	0.0011005103380541882	2.462851638451245
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	7797	0.8603567219713723	No Hit
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	7248	0.7997775453185206	No Hit
CTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGCT	7182	0.7924948027700905	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	3326	0.36700608660725714	No Hit
ACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	2042	0.22532364066506888	No Hit
GCCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTG	1534	0.16926859195896948	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCC	1402	0.15470310686210897	No Hit
ACCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTG	1294	0.14278589178285953	No Hit
CGCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTG	1147	0.12656523792499216	No Hit
GGCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTG	1009	0.11133768532372894	No Hit
GGCCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCT	968	0.10681355737697683	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8169913004329922	0.0
2	0.0	0.0	0.0	3.153758557222494	0.0
3	0.0	0.0	0.0	4.145094300481544	0.0
4	1.1034458406712482E-4	0.0	0.0	5.156843791793011	0.0
5	1.1034458406712482E-4	0.0	0.0	7.326218314552685	0.0
6	1.1034458406712482E-4	0.0	0.0	8.29702996517525	0.0
7	1.1034458406712482E-4	0.0	0.0	9.476503224268747	0.0
8	1.1034458406712482E-4	0.0	0.0	11.581105476181017	0.0
9	2.2068916813424965E-4	0.0	0.0	12.299669407626135	0.0
10	2.2068916813424965E-4	0.0	0.0	14.400740632848258	0.0
11	2.2068916813424965E-4	0.0	0.0	17.4616993948703	0.0
12	2.2068916813424965E-4	0.0	0.0	19.70963926148577	0.0
13	2.2068916813424965E-4	0.0	0.0	20.538437432413943	0.0
14	2.2068916813424965E-4	0.0	0.0	20.83669884314738	0.0
15	2.2068916813424965E-4	0.0	0.0	21.457056094772756	0.0
16	2.2068916813424965E-4	0.0	0.0	22.889108106795902	0.0
17	2.2068916813424965E-4	0.0	0.0	24.76430396843262	0.0
18	2.2068916813424965E-4	0.0	0.0	26.816602887497076	0.0
19	2.2068916813424965E-4	0.0	0.0	27.966724487228717	0.0
20	2.2068916813424965E-4	0.0	0.0	29.028018696786326	0.0
21	6.620675044027489E-4	0.0	0.0	30.326774451256384	0.0
22	6.620675044027489E-4	0.0	0.0	31.687543861972166	0.0
23	8.827566725369986E-4	0.0	0.0	33.038161570953775	0.0
24	0.0014344795928726225	0.0	0.0	34.031483516726034	0.0
25	0.001875857929141122	0.0	0.0	34.898791947493635	0.0
26	0.001875857929141122	0.0	0.0	35.722403922970656	0.0
27	0.001875857929141122	0.0	0.0	36.52317456954578	0.0
28	0.001875857929141122	0.0	0.0	37.33398657327101	0.0
29	0.001875857929141122	0.0	0.0	38.222591508763564	0.0
30	0.001875857929141122	0.0	0.0	39.1538997982901	0.0
31	0.001875857929141122	0.0	0.0	40.00785653438558	0.0
32	0.001875857929141122	0.0	0.0	40.81767543685421	0.0
33	0.001875857929141122	0.0	0.0	41.57265308104147	0.0
34	0.0019862025132082466	0.0	0.0	42.325423833547404	0.0
35	0.0026482700176109954	0.0	0.0	43.09331179407052	0.0
36	0.002868959185745245	0.0	0.0	43.86186182209805	0.0
37	0.002868959185745245	0.0	0.0	44.62048083755953	0.0
38	0.00297930376981237	0.0	0.0	45.36188609790654	0.0
39	0.00297930376981237	0.0	0.0	46.1383809359869	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTC	35	1.2115561E-7	45.0	23
GACGTAA	25	3.8901497E-5	45.0	16
TAGCGCG	50	2.1827873E-11	45.0	1
GATCCGC	35	1.2115561E-7	45.0	9
GCACGCG	45	3.8380676E-10	45.0	1
TTCCGGT	20	7.0324965E-4	45.0	24
CGATTCA	25	3.8901497E-5	45.0	10
CGATTAT	20	7.0324965E-4	45.0	10
CTGCGAC	20	7.0324965E-4	45.0	29
GAGACGT	25	3.8901497E-5	45.0	14
TGCGACA	20	7.0324965E-4	45.0	30
CGAATCG	25	3.8901497E-5	45.0	2
TCCGTAG	25	3.8901497E-5	45.0	1
CTCGCAG	20	7.0324965E-4	45.0	1
ACGTAAG	50	2.1827873E-11	45.0	1
GCGTGAT	20	7.0324965E-4	45.0	13
ATACGCG	25	3.8901497E-5	45.0	1
TATCGCG	40	6.8121153E-9	45.0	1
TGATCCG	30	2.164992E-6	44.999996	1
GTGTCGA	30	2.164992E-6	44.999996	9
>>END_MODULE