FastQCFastQC Report
Sat 18 Jun 2016
SRR3552780_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552780_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2127849
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC103500.48640669521192526Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC86460.406325824811817Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCT86420.406137841547967Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC30770.14460612571662743Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC22740.10686848549873605Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGTA253.891929E-545.09
CACAACG7250.036.93103812
CGCATGG5150.036.6990282
ACAACGA7250.036.6206913
TCGTCGA250.002107510636.09
CGACCAA6300.035.71428729
ACACGAC6450.035.58139826
GGACTAA9650.035.4404148
TTACGCG702.1827873E-1135.3571431
ACGGGAG13600.035.238975
CGAATGG2750.035.181822
CGTAAGG3400.035.073532
TCGTAAG900.035.01
TACGGGA3550.034.8591584
ATAGCGG3100.034.838712
AACGGGA6950.034.6402854
CGTTAGG2750.034.3636362
TGCTACG1900.034.3421061
GCGTATG2900.034.1379281
GCGTACG1450.034.1379281
Produced by FastQC (version 0.11.5)