##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552780_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2127849
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.001588928537693	33.0	33.0	33.0	27.0	33.0
2	31.237017288350817	33.0	33.0	33.0	27.0	33.0
3	31.41813164373976	33.0	33.0	33.0	27.0	33.0
4	31.387490371732206	33.0	33.0	33.0	27.0	33.0
5	31.51377423867953	33.0	33.0	33.0	27.0	33.0
6	34.74940797020841	37.0	37.0	37.0	27.0	37.0
7	35.03194399602604	37.0	37.0	37.0	27.0	37.0
8	35.10928454039737	37.0	37.0	37.0	33.0	37.0
9	35.01204314779855	37.0	37.0	37.0	33.0	37.0
10	35.048449866508385	37.0	37.0	37.0	27.0	37.0
11	35.05699746551564	37.0	37.0	37.0	27.0	37.0
12	35.06267550000023	37.0	37.0	37.0	27.0	37.0
13	35.14050198110862	37.0	37.0	37.0	33.0	37.0
14	35.1904138874516	37.0	37.0	37.0	33.0	37.0
15	35.222867788080826	37.0	37.0	37.0	33.0	37.0
16	35.250905021925895	37.0	37.0	37.0	33.0	37.0
17	35.2637611973406	37.0	37.0	37.0	33.0	37.0
18	35.257211860428065	37.0	37.0	37.0	33.0	37.0
19	35.25063902560755	37.0	37.0	37.0	33.0	37.0
20	35.220486510086005	37.0	37.0	37.0	33.0	37.0
21	35.16642346331906	37.0	37.0	37.0	33.0	37.0
22	35.19765453281694	37.0	37.0	37.0	33.0	37.0
23	35.20229489968508	37.0	37.0	37.0	33.0	37.0
24	35.20663214354026	37.0	37.0	37.0	33.0	37.0
25	35.18901529196856	37.0	37.0	37.0	33.0	37.0
26	35.135262887545124	37.0	37.0	37.0	33.0	37.0
27	35.13722590277788	37.0	37.0	37.0	33.0	37.0
28	35.115518065426635	37.0	37.0	37.0	33.0	37.0
29	35.10126517436153	37.0	37.0	37.0	33.0	37.0
30	35.088731860202486	37.0	37.0	37.0	33.0	37.0
31	35.07205727474083	37.0	37.0	37.0	33.0	37.0
32	35.059037553886576	37.0	37.0	37.0	33.0	37.0
33	35.044577411273075	37.0	37.0	37.0	33.0	37.0
34	35.01225368905406	37.0	37.0	37.0	27.0	37.0
35	35.00036280769923	37.0	37.0	37.0	27.0	37.0
36	34.98887468048719	37.0	37.0	37.0	27.0	37.0
37	34.98337663997774	37.0	37.0	37.0	27.0	37.0
38	34.96857624765667	37.0	37.0	37.0	27.0	37.0
39	34.957593795424394	37.0	37.0	37.0	27.0	37.0
40	34.92979342049178	37.0	37.0	37.0	27.0	37.0
41	34.91306995938152	37.0	37.0	37.0	27.0	37.0
42	34.90229099903235	37.0	37.0	37.0	27.0	37.0
43	34.89263100906126	37.0	37.0	37.0	27.0	37.0
44	34.867621715638656	37.0	37.0	37.0	27.0	37.0
45	34.821901366121374	37.0	37.0	37.0	27.0	37.0
46	34.84231164899389	37.0	37.0	37.0	27.0	37.0
47	34.80462147455012	37.0	37.0	37.0	27.0	37.0
48	34.79829254801445	37.0	37.0	37.0	27.0	37.0
49	34.785479608750435	37.0	37.0	37.0	27.0	37.0
50	34.76283373491258	37.0	37.0	37.0	27.0	37.0
51	34.32721776780213	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1367.0
15	6375.0
16	7752.0
17	7263.0
18	7013.0
19	6803.0
20	7073.0
21	7918.0
22	8670.0
23	10359.0
24	12394.0
25	15372.0
26	18864.0
27	24262.0
28	29771.0
29	35558.0
30	42262.0
31	51255.0
32	65216.0
33	86745.0
34	128472.0
35	260633.0
36	1286452.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.70127015591802	17.201596541859878	20.840764546732405	14.256368755489698
2	30.420015706001696	22.935415059997208	24.28762567268636	22.356943561314736
3	32.300882252453064	24.86191454374817	24.250263998996168	18.586939204802597
4	29.26922916052784	26.02398008505303	24.522322777603108	20.184467976816023
5	25.77353938178884	29.36777938660121	24.01349907817707	20.84518215343288
6	26.873100487863567	34.7444297034235	22.540744197544093	15.841725611168838
7	80.03580141260024	6.826894201609231	7.786971725907242	5.35033265988329
8	80.53964355553424	5.676154651951337	7.654208545813167	6.129993246701247
9	75.57293774135289	7.359215809016523	10.188974875566828	6.87887157406376
10	40.06280520845229	26.05499732358828	17.2875518892553	16.594645578704128
11	31.35744124700578	26.438530177658283	22.71180896764761	19.492219607688327
12	27.216028956941962	24.96831307108728	28.255858380928345	19.55979959104241
13	25.56055434384677	24.974751497874145	29.425490248603165	20.03920390967592
14	21.885246556499073	27.309127668363686	28.631448942100686	22.174176833036555
15	20.970613986236806	25.945356084947758	31.652292996354536	21.431736932460904
16	23.89699645040602	26.067968168793932	28.45107900043659	21.583956380363457
17	23.710799027562576	25.536633473521853	27.085803550909866	23.66676394800571
18	24.802558828187525	24.095412785399716	29.42633617329049	21.675692213122268
19	24.0557013209114	26.0995963529367	27.094356789415038	22.750345536736866
20	26.36939933237744	26.35144693067976	27.254565526031215	20.02458821091158
21	24.847204853351908	26.999566228618665	28.13136646444367	20.021862453585758
22	23.430844951873937	24.844761070921855	27.206864772829277	24.517529204374934
23	22.30332133530152	26.903318797527454	27.69952191156421	23.093837955606812
24	22.922021252447895	25.235625272282007	28.47481188749766	23.367541587772443
25	22.20660394605068	27.078472203619715	25.898125289905437	24.816798560424168
26	21.552046221324915	26.335515349068473	26.99528960936608	25.11714882024053
27	22.135546272315377	25.6407292058788	27.693929409464673	24.529795112341148
28	19.203054352071035	26.462451047983198	28.45615454856054	25.878340051385223
29	21.113575258394746	25.864241306596476	27.075840437925812	25.946342997082972
30	22.82943949500176	25.10944150642268	27.817575401262022	24.24354359731353
31	22.620684080496314	27.041580488089146	25.979146076624797	24.358589354789743
32	23.60679728683755	26.275125725556652	25.304380151035154	24.81369683657064
33	22.174787778644067	26.589809709241585	25.95292241131772	25.28248010079663
34	20.123702386776504	25.71822530640097	29.416279068674516	24.74179323814801
35	21.19920163507843	25.7623073817738	26.589433742713886	26.44905724043388
36	21.815269786530905	28.386694732567957	25.91372790080499	23.884307580096145
37	20.905665768576622	27.913399870009574	26.857027918804388	24.323906442609413
38	21.017891777095084	28.114165995801393	26.76186139148032	24.106080835623203
39	21.769260882703612	26.158529106153676	27.480568404994905	24.591641606147803
40	23.966362274766677	24.86905790777447	27.856158966167243	23.308420851291608
41	20.782818705650634	26.79565138315736	25.6869730887859	26.734556822406102
42	22.319910858336282	25.61318965772477	26.54427076357392	25.522628720365027
43	22.40017971200024	23.830168400107336	28.12821774477418	25.641434143118236
44	21.006659777080046	24.129296768708684	28.148190966558246	26.715852487653024
45	21.87086583681455	23.295919964245584	27.428638028356332	27.404576170583532
46	21.77598128438625	24.752226309291682	27.945592003943887	25.526200402378173
47	19.498329063763453	26.341013859536083	29.628841144272926	24.531815932427538
48	20.55831969279775	25.55557278735474	28.481109326836634	25.404998193010876
49	22.564618072053044	23.95080665968309	29.448659185872682	24.035916082391186
50	20.452767090145965	25.043976334786915	28.4381551510469	26.06510142402022
51	20.14442754161597	23.852961370849155	26.927286663668333	29.075324423866544
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	411.0
1	664.5
2	918.0
3	1956.5
4	2995.0
5	2518.0
6	2041.0
7	2038.5
8	2036.0
9	2093.0
10	2150.0
11	2269.5
12	2389.0
13	2753.0
14	3117.0
15	3349.5
16	3582.0
17	3668.0
18	3754.0
19	4289.0
20	4824.0
21	5192.0
22	5560.0
23	6556.5
24	7553.0
25	9132.0
26	13567.5
27	16424.0
28	19272.0
29	22120.0
30	25998.0
31	29876.0
32	35224.5
33	40573.0
34	45514.5
35	50456.0
36	55484.0
37	60512.0
38	65411.0
39	70310.0
40	76933.5
41	83557.0
42	92831.0
43	102105.0
44	114353.0
45	126601.0
46	146142.5
47	165684.0
48	192988.0
49	220292.0
50	224691.5
51	229091.0
52	206198.5
53	183306.0
54	166163.0
55	149020.0
56	132661.0
57	116302.0
58	106172.0
59	96042.0
60	90329.5
61	84617.0
62	78824.0
63	73031.0
64	62662.0
65	52293.0
66	44278.5
67	36264.0
68	30354.5
69	24445.0
70	20675.5
71	16906.0
72	13444.0
73	9982.0
74	8645.0
75	5603.5
76	3899.0
77	3182.5
78	2466.0
79	1819.5
80	1173.0
81	928.5
82	684.0
83	504.5
84	325.0
85	214.0
86	103.0
87	68.0
88	33.0
89	20.0
90	7.0
91	3.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2127849.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.48949531937028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.89288447090358	18.070238468977546
2	11.25158147727568	5.735942668010636
3	4.891682225326705	3.7405953355893558
4	2.6785003963084453	2.7309449325854223
5	1.5914432806162269	2.0282543026155295
6	0.9907510243382557	1.5152246158518767
7	0.7056697732603683	1.259101646777887
8	0.5259837475887358	1.0725648217782335
9	0.38918467468283446	0.8928108849328878
>10	4.422715013034866	29.21784963935643
>50	1.5490349260473297	26.192702457198337
>100	0.10203521524849195	4.273852720148301
>500	0.0059365393867022	1.0711521525659313
>1k	0.0020406854141788813	0.8918386719668667
>5k	3.710337116688875E-4	0.817503020199533
>10k+	1.8551685583444375E-4	0.48942366144523175
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	10350	0.48640669521192526	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	8646	0.406325824811817	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCT	8642	0.406137841547967	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	3077	0.14460612571662743	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	2274	0.10686848549873605	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8798326385001943E-4	0.0	0.0	0.42512415119681896	0.0
2	1.8798326385001943E-4	0.0	0.0	1.6236114498726177	0.0
3	1.8798326385001943E-4	0.0	0.0	2.137745676502421	0.0
4	2.3497907981252428E-4	0.0	0.0	2.65798935920735	0.0
5	2.3497907981252428E-4	0.0	0.0	3.9256544989799558	0.0
6	2.3497907981252428E-4	0.0	0.0	4.501212257072753	0.0
7	2.3497907981252428E-4	0.0	0.0	5.1921447433534995	0.0
8	2.3497907981252428E-4	0.0	0.0	6.430578485597427	0.0
9	2.3497907981252428E-4	0.0	0.0	6.86947241087126	0.0
10	2.3497907981252428E-4	0.0	0.0	8.13121607783259	0.0
11	2.3497907981252428E-4	0.0	0.0	9.89332419734671	0.0
12	2.3497907981252428E-4	0.0	0.0	11.18956279322452	0.0
13	2.3497907981252428E-4	0.0	0.0	11.702099162111598	0.0
14	2.3497907981252428E-4	0.0	0.0	11.910854576617044	0.0
15	2.3497907981252428E-4	0.0	0.0	12.362061405673053	0.0
16	2.3497907981252428E-4	0.0	0.0	13.270678511492122	0.0
17	2.3497907981252428E-4	0.0	0.0	14.32578157566632	0.0
18	2.3497907981252428E-4	0.0	0.0	15.42863238885842	0.0
19	2.3497907981252428E-4	0.0	0.0	16.114677310279067	0.0
20	2.3497907981252428E-4	0.0	0.0	16.76373652453722	0.0
21	4.229623436625437E-4	0.0	0.0	17.59227275995618	0.0
22	4.6995815962504856E-4	0.0	0.0	18.41662636775448	0.0
23	6.109456075125631E-4	0.0	0.0	19.2335546366307	0.0
24	7.989288713625826E-4	0.0	0.0	19.92063346600252	0.0
25	0.001268887030987631	0.0	0.0	20.50690627013477	0.0
26	0.001315882846950136	0.0	0.0	21.040731743652863	0.0
27	0.001315882846950136	0.0	0.0	21.590676782046096	0.0
28	0.0014098744788751457	0.0	0.0	22.13319648151725	0.0
29	0.0014098744788751457	0.0	0.0	22.70311474169455	0.0
30	0.0014568702948376507	0.0	0.0	23.37393301874334	0.0
31	0.0015038661108001555	0.0	0.0	23.930880433714986	0.0
32	0.0015038661108001555	0.0	0.0	24.477018811015256	0.0
33	0.0015508619267626603	0.0	0.0	25.015402878681712	0.0
34	0.0015508619267626603	0.0	0.0	25.552236084421406	0.0
35	0.0015508619267626603	0.0	0.0	26.148049039194042	0.0
36	0.0016918493746501749	0.0	0.0	26.67562406918912	0.0
37	0.0017388451906126797	0.0	0.0	27.187361509204834	0.0
38	0.0017388451906126797	0.0	0.0	27.742570078985867	0.0
39	0.0017388451906126797	0.0	0.0	28.436322314224366	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGTA	25	3.891929E-5	45.0	9
CACAACG	725	0.0	36.931038	12
CGCATGG	515	0.0	36.699028	2
ACAACGA	725	0.0	36.62069	13
TCGTCGA	25	0.0021075106	36.0	9
CGACCAA	630	0.0	35.714287	29
ACACGAC	645	0.0	35.581398	26
GGACTAA	965	0.0	35.440414	8
TTACGCG	70	2.1827873E-11	35.357143	1
ACGGGAG	1360	0.0	35.23897	5
CGAATGG	275	0.0	35.18182	2
CGTAAGG	340	0.0	35.07353	2
TCGTAAG	90	0.0	35.0	1
TACGGGA	355	0.0	34.859158	4
ATAGCGG	310	0.0	34.83871	2
AACGGGA	695	0.0	34.640285	4
CGTTAGG	275	0.0	34.363636	2
TGCTACG	190	0.0	34.342106	1
GCGTATG	290	0.0	34.137928	1
GCGTACG	145	0.0	34.137928	1
>>END_MODULE