SRR3552777_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552777_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1673976
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	7115	0.4250359622838081	RNA PCR Primer, Index 35 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	7023	0.419540065090539	RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	5032	0.300601681266637	TruSeq Adapter, Index 10 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	3221	0.19241613977739228	RNA PCR Primer, Index 35 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2294	0.13703900175390807	No Hit
ACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	2148	0.12831725186024173	TruSeq Adapter, Index 10 (95% over 24bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2046	0.12222397453726934	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1749	0.1044817846850851	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1718	0.10262990628300526	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTTA	30	2.166098E-6	45.000004	13
GTCGACG	45	3.8562575E-10	45.0	1
AACCCGC	25	3.8915718E-5	45.0	22
ATAGACG	50	2.1827873E-11	45.0	1
TATCTCG	40	6.8175723E-9	45.0	1
CGCACGA	20	7.03421E-4	45.0	26
GCTACGA	320	0.0	45.0	10
GCGTAAT	20	7.03421E-4	45.0	34
GTACGAG	55	1.8189894E-12	45.0	1
TGCGTAG	120	0.0	43.125004	1
CGAATAT	320	0.0	42.890625	14
CTACGAA	365	0.0	42.534245	11
ACGGGTA	165	0.0	40.909092	5
CGGGTAC	105	0.0	40.714287	6
CGATATT	50	1.0822987E-9	40.5	10
AACACGT	510	0.0	40.14706	41
ACATGCG	45	1.9292202E-8	40.0	1
TACGAAT	345	0.0	39.782608	12
GCGAGAC	555	0.0	39.72973	21
ACGGGCC	290	0.0	39.56897	5