##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552777_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1673976
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.757012047962455	31.0	31.0	34.0	30.0	34.0
2	31.92686573762109	33.0	31.0	34.0	30.0	34.0
3	31.970471500188772	33.0	31.0	34.0	30.0	34.0
4	35.58292472532462	37.0	35.0	37.0	33.0	37.0
5	35.455983239903084	37.0	35.0	37.0	33.0	37.0
6	35.427113052994784	37.0	35.0	37.0	33.0	37.0
7	35.79147490764503	37.0	35.0	37.0	35.0	37.0
8	35.74352320463376	37.0	35.0	37.0	35.0	37.0
9	37.33209556170459	39.0	37.0	39.0	35.0	39.0
10	36.80232870722161	39.0	37.0	39.0	32.0	39.0
11	36.639998422916456	39.0	35.0	39.0	32.0	39.0
12	36.49367254966619	38.0	35.0	39.0	32.0	39.0
13	36.421799954121205	39.0	35.0	39.0	32.0	39.0
14	37.49270479385606	39.0	36.0	41.0	32.0	41.0
15	37.58342592725344	39.0	36.0	41.0	33.0	41.0
16	37.633060450090085	39.0	36.0	41.0	33.0	41.0
17	37.6172770696832	39.0	36.0	41.0	33.0	41.0
18	37.58022217761784	39.0	36.0	41.0	32.0	41.0
19	37.55967110639579	39.0	36.0	41.0	32.0	41.0
20	37.50631371059083	39.0	36.0	41.0	32.0	41.0
21	37.42941953767557	39.0	36.0	41.0	32.0	41.0
22	37.366678494793234	39.0	36.0	41.0	32.0	41.0
23	37.289584199534524	39.0	35.0	41.0	32.0	41.0
24	37.262106505708566	39.0	35.0	41.0	32.0	41.0
25	37.185607798439165	39.0	35.0	41.0	32.0	41.0
26	37.054102328826694	39.0	35.0	41.0	31.0	41.0
27	37.00581489818253	39.0	35.0	41.0	31.0	41.0
28	36.90521309743987	39.0	35.0	40.0	31.0	41.0
29	36.88705154673663	39.0	35.0	40.0	31.0	41.0
30	36.83071680836523	39.0	35.0	40.0	31.0	41.0
31	36.69633017438721	39.0	35.0	40.0	30.0	41.0
32	36.60714848958408	39.0	35.0	40.0	30.0	41.0
33	36.508305973323395	39.0	35.0	40.0	30.0	41.0
34	36.40285404330767	38.0	35.0	40.0	30.0	41.0
35	36.40942761425492	39.0	35.0	40.0	30.0	41.0
36	36.37532676693095	38.0	35.0	40.0	30.0	41.0
37	36.31422075346361	38.0	35.0	40.0	30.0	41.0
38	36.24786735293696	38.0	35.0	40.0	30.0	41.0
39	36.19671906885164	38.0	35.0	40.0	30.0	41.0
40	36.079279511773166	38.0	35.0	40.0	30.0	41.0
41	35.9767648998552	38.0	35.0	40.0	29.0	41.0
42	35.913442606106656	38.0	35.0	40.0	29.0	41.0
43	35.84015899869532	38.0	34.0	40.0	29.0	41.0
44	35.70308236199324	38.0	34.0	40.0	29.0	41.0
45	35.642536691087564	38.0	34.0	40.0	29.0	41.0
46	35.512691938235676	38.0	34.0	40.0	28.0	41.0
47	35.42350607177164	38.0	34.0	40.0	28.0	41.0
48	35.37028069697534	37.0	34.0	40.0	28.0	41.0
49	35.32410500508968	37.0	34.0	40.0	28.0	41.0
50	35.2121398395198	37.0	34.0	40.0	27.0	41.0
51	33.64137598149555	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	9.0
11	15.0
12	15.0
13	21.0
14	27.0
15	80.0
16	168.0
17	275.0
18	562.0
19	964.0
20	1664.0
21	2478.0
22	3709.0
23	5539.0
24	7893.0
25	10839.0
26	14534.0
27	18483.0
28	23250.0
29	29592.0
30	37351.0
31	47403.0
32	61423.0
33	81580.0
34	125339.0
35	142647.0
36	153455.0
37	213187.0
38	326471.0
39	364934.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.94393945910814	20.616544084264053	24.716363914416934	10.72315254221088
2	30.764598775609684	24.28696707718629	25.449409071575698	19.499025075628325
3	31.789045960037658	24.35972797698414	26.162083566311583	17.68914249666662
4	29.208901441836684	26.049357935836593	25.272823505235444	19.468917117091284
5	25.436386184748166	30.198760316754843	24.172389568309224	20.192463930187767
6	26.19493947344526	35.24716005486339	22.90648133545523	15.651419136236122
7	81.58283033926412	6.00253528127046	7.522807973352068	4.891826406113349
8	81.73438567816982	5.372299244433612	7.46306996038175	5.430245117014819
9	76.10318188552286	7.47633179926116	9.850559386753455	6.569926928462534
10	41.34485799079557	26.054973309055807	16.39623268195004	16.20393601819859
11	30.909284243023794	26.69823223272018	21.70084875768828	20.69163476656774
12	27.78725620916907	23.169985710667298	28.549513254670316	20.493244825493317
13	24.669708526287117	25.562074964037716	29.17855453124776	20.589661978427408
14	20.17962025739915	27.990305715255175	28.36229432202134	23.46777970532433
15	20.321975942307414	26.462207343474457	32.14627927760016	21.06953743661797
16	22.18054500183993	25.47420034695838	30.24195090013238	22.103303751069312
17	22.223078467074796	25.739616338585495	27.190234507543714	24.847070686795988
18	22.9218340047886	25.350901088187644	28.925265356253615	22.80199955077014
19	24.377529904849293	26.732701066204058	25.70042820207697	23.18934082686968
20	26.66065702256185	25.88101621528624	26.53072684435141	20.927599917800492
21	25.02401468121407	26.94208280166502	26.42522951344583	21.608673003675083
22	22.855644286417487	26.23281337366844	25.050179930895066	25.861362409019005
23	23.062098859242905	27.148358160451526	26.341058653170656	23.448484327134917
24	23.362401850444687	24.14765803093951	28.413191109072056	24.076749009543743
25	22.20139356836657	26.553606503319045	26.07080388249294	25.174196045821446
26	21.261893838382388	26.8441124603937	26.668363226235027	25.225630474988886
27	22.91293304085602	26.34356764971541	27.27171715723523	23.47178215219334
28	19.878779624080632	26.977806133421268	27.515687202206006	25.627727040292097
29	20.965473818023675	25.23895205188127	27.77094773162817	26.024626398466882
30	22.518781631277868	24.856449554832327	27.86861938283464	24.756149431055164
31	22.36429912973663	26.465552672200797	24.497065668802897	26.673082529259677
32	22.79214277863004	25.40801062858727	26.760897408326045	25.038949184456648
33	21.823251946264463	25.448333787342232	27.245551907554226	25.482862358839075
34	20.144852733850428	24.564450147433416	27.955597929719424	27.335099188996736
35	20.338164943822374	23.493706002953445	29.040022079169592	27.12810697405459
36	21.38453597901045	26.092548519214137	26.95409014227205	25.56882535950336
37	20.76702413893628	25.947743575774084	28.12830052521661	25.156931760073025
38	20.311641266063553	26.913707245504114	28.187620372096134	24.587031116336195
39	21.793502415805243	23.75750906823037	28.48224825206574	25.966740263898647
40	23.039816580404977	23.162040554942244	29.768825837407466	24.029317027245312
41	18.80958866793789	24.470123824953287	28.265399264983486	28.454888242125335
42	20.667500609327732	23.921848341911712	28.17011713429583	27.240533914464727
43	22.608508126759286	22.17845417138597	28.561879023355175	26.65115867849957
44	21.17897747637959	23.649084574689244	27.715809545656565	27.456128403274597
45	21.85443518903497	22.490167123065085	27.696573905480125	27.958823782419817
46	21.13722060531334	24.225257709787954	28.205123609896436	26.432398075002272
47	19.44113894106009	23.455832102730266	30.93467289853618	26.16835605767347
48	19.602252362041035	22.58670375202512	30.634489383360336	27.176554502573513
49	20.709556170458836	22.39070333146951	31.091305968544354	25.808434529527307
50	19.3344468498951	22.921176886645924	30.417222230187292	27.327154033271682
51	19.482895812126337	22.10891912428852	27.62912968883664	30.779055374748506
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	451.0
1	774.0
2	1097.0
3	1606.0
4	2115.0
5	1791.0
6	1467.0
7	1437.5
8	1408.0
9	1508.0
10	1608.0
11	1720.0
12	1832.0
13	2176.5
14	2521.0
15	2398.5
16	2276.0
17	2619.5
18	2963.0
19	3045.0
20	3127.0
21	3547.5
22	3968.0
23	4514.5
24	5061.0
25	7393.5
26	12200.0
27	14674.0
28	16249.5
29	17825.0
30	20673.0
31	23521.0
32	27166.5
33	30812.0
34	35339.0
35	39866.0
36	43839.5
37	47813.0
38	49776.5
39	51740.0
40	59299.5
41	66859.0
42	74877.0
43	82895.0
44	94516.5
45	106138.0
46	120615.0
47	135092.0
48	155159.5
49	175227.0
50	173735.5
51	172244.0
52	149687.5
53	127131.0
54	115367.5
55	103604.0
56	95534.0
57	87464.0
58	85084.5
59	82705.0
60	77146.5
61	71588.0
62	67735.5
63	63883.0
64	54255.5
65	44628.0
66	36433.0
67	28238.0
68	24518.0
69	20798.0
70	18107.5
71	15417.0
72	12435.5
73	9454.0
74	7732.5
75	5029.5
76	4048.0
77	3283.0
78	2518.0
79	1829.0
80	1140.0
81	811.5
82	483.0
83	329.5
84	176.0
85	165.5
86	155.0
87	109.0
88	63.0
89	50.0
90	37.0
91	29.0
92	21.0
93	26.0
94	31.0
95	43.5
96	56.0
97	28.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1673976.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.796249898928906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.05631458937451	14.615761900911991
2	12.896813986193244	5.622043610861388
3	5.746712987152425	3.757703770961835
4	2.9839469633450006	2.6015541479287076
5	1.7537615422079447	1.9112712418547662
6	1.105789214825057	1.4461234837120394
7	0.7709279873883235	1.1762337347035947
8	0.5662137396673439	0.9873068932797203
9	0.4216508123509434	0.8271365828478788
>10	4.3407293475921245	23.990070602526554
>50	2.1041451280423935	31.714577607641825
>100	0.242566629631929	8.001795833879031
>500	0.005762329389626533	0.8358790795973162
>1k	0.003841552926417689	1.3660187591587507
>5k	8.231899128037906E-4	1.1465227501345556
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	7115	0.4250359622838081	RNA PCR Primer, Index 35 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	7023	0.419540065090539	RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	5032	0.300601681266637	TruSeq Adapter, Index 10 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	3221	0.19241613977739228	RNA PCR Primer, Index 35 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2294	0.13703900175390807	No Hit
ACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	2148	0.12831725186024173	TruSeq Adapter, Index 10 (95% over 24bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2046	0.12222397453726934	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1749	0.1044817846850851	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1718	0.10262990628300526	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.389520518812695E-4	0.0	0.0	0.3155959225221867	0.0
2	2.389520518812695E-4	0.0	0.0	1.5465574177885466	0.0
3	2.389520518812695E-4	0.0	0.0	2.1131724708120068	0.0
4	2.389520518812695E-4	0.0	0.0	2.7476499065697477	0.0
5	2.389520518812695E-4	0.0	0.0	4.342057472747518	0.0
6	2.389520518812695E-4	0.0	0.0	5.169309476360474	0.0
7	2.389520518812695E-4	0.0	0.0	6.052774950178497	0.0
8	2.389520518812695E-4	0.0	0.0	7.392459629050835	0.0
9	2.389520518812695E-4	0.0	0.0	7.802620826105033	0.0
10	2.389520518812695E-4	0.0	0.0	8.928622632582545	0.0
11	2.389520518812695E-4	0.0	0.0	10.600510401582818	0.0
12	2.389520518812695E-4	0.0	0.0	11.85076727503859	0.0
13	2.389520518812695E-4	0.0	0.0	12.341634527615689	0.0
14	2.986900648515869E-4	0.0	0.0	12.535125951626547	0.0
15	2.986900648515869E-4	0.0	0.0	12.931965571788364	0.0
16	3.5842807782190424E-4	0.0	0.0	13.782814090524596	0.0
17	3.5842807782190424E-4	0.0	0.0	14.78336606976444	0.0
18	4.1816609079222165E-4	0.0	0.0	15.884098696755508	0.0
19	4.1816609079222165E-4	0.0	0.0	16.51110888089196	0.0
20	4.1816609079222165E-4	0.0	0.0	17.113148575606818	0.0
21	0.0018518784020798386	0.0	0.0	17.886755843572427	0.0
22	0.001911616415050156	0.0	0.0	18.712932562951917	0.0
23	0.0019713544280204735	0.0	0.0	19.484329524437626	0.0
24	0.002329782505842378	0.0	0.0	20.133383035360126	0.0
25	0.0025687345577236472	0.0	0.0	20.705255033524974	0.0
26	0.0025687345577236472	0.0	0.0	21.21691111461574	0.0
27	0.0026284725706939645	0.0	0.0	21.706523868920463	0.0
28	0.0026882105836642817	0.0	0.0	22.19350815065449	0.0
29	0.0026882105836642817	0.0	0.0	22.711795151184962	0.0
30	0.0026882105836642817	0.0	0.0	23.308099996654672	0.0
31	0.0026882105836642817	0.0	0.0	23.869876270627536	0.0
32	0.0027479485966345994	0.0	0.0	24.42406581695317	0.0
33	0.0027479485966345994	0.0	0.0	24.966964878827415	0.0
34	0.0027479485966345994	0.0	0.0	25.473065324711943	0.0
35	0.0028076866096049167	0.0	0.0	26.027613299115398	0.0
36	0.002867424622575234	0.0	0.0	26.562686681290533	0.0
37	0.002867424622575234	0.0	0.0	27.095370542946853	0.0
38	0.0029271626355455516	0.0	0.0	27.594840069391676	0.0
39	0.0029271626355455516	0.0	0.0	28.11868270512839	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTTA	30	2.166098E-6	45.000004	13
GTCGACG	45	3.8562575E-10	45.0	1
AACCCGC	25	3.8915718E-5	45.0	22
ATAGACG	50	2.1827873E-11	45.0	1
TATCTCG	40	6.8175723E-9	45.0	1
CGCACGA	20	7.03421E-4	45.0	26
GCTACGA	320	0.0	45.0	10
GCGTAAT	20	7.03421E-4	45.0	34
GTACGAG	55	1.8189894E-12	45.0	1
TGCGTAG	120	0.0	43.125004	1
CGAATAT	320	0.0	42.890625	14
CTACGAA	365	0.0	42.534245	11
ACGGGTA	165	0.0	40.909092	5
CGGGTAC	105	0.0	40.714287	6
CGATATT	50	1.0822987E-9	40.5	10
AACACGT	510	0.0	40.14706	41
ACATGCG	45	1.9292202E-8	40.0	1
TACGAAT	345	0.0	39.782608	12
GCGAGAC	555	0.0	39.72973	21
ACGGGCC	290	0.0	39.56897	5
>>END_MODULE