FastQCFastQC Report
Sat 18 Jun 2016
SRR3552774_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552774_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819383
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC70240.8572303794440451TruSeq Adapter, Index 27 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC60650.7401910950068528TruSeq Adapter, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT53810.6567136491726091TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC22280.27191191420861793TruSeq Adapter, Index 27 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC15980.19502479304549886TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCC13210.16121886834361954No Hit
GCCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTG13210.16121886834361954TruSeq Adapter, Index 27 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTG11890.1451091858142041No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10350.12631455619655277No Hit
CGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTG10270.12533821180083063No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8310.10141777410563803No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA302.1647356E-645.00000418
TCCGCTA351.211356E-745.026
ATAGCCG253.8898223E-545.01
TAACGCG253.8898223E-545.01
TATTGCG253.8898223E-545.01
ACGATTG207.0321007E-445.01
GCGATTC207.0321007E-445.09
CTCGACG253.8898223E-545.01
TGCTACG207.0321007E-445.016
ACGTACG253.8898223E-545.01
CCGAAAT207.0321007E-445.034
CCAACGG207.0321007E-445.02
CGACTAT207.0321007E-445.032
TATACGG351.211356E-745.02
CGATCTA253.8898223E-545.011
ACGGTTG207.0321007E-445.01
ATAGGGA5800.041.5086254
CGGGATC1200.041.2500046
TTACGGG1800.041.253
TAAACGG556.002665E-1140.9090922
Produced by FastQC (version 0.11.5)