##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552774_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	819383
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.860773289170023	33.0	27.0	33.0	27.0	33.0
2	31.088479990431825	33.0	33.0	33.0	27.0	33.0
3	31.302179810906498	33.0	33.0	33.0	27.0	33.0
4	31.273597328721735	33.0	33.0	33.0	27.0	33.0
5	31.405519763041216	33.0	33.0	33.0	27.0	33.0
6	34.72726429520749	37.0	37.0	37.0	27.0	37.0
7	35.16138118559941	37.0	37.0	37.0	33.0	37.0
8	35.26681295560196	37.0	37.0	37.0	33.0	37.0
9	35.18205527817882	37.0	37.0	37.0	33.0	37.0
10	35.184946478020656	37.0	37.0	37.0	33.0	37.0
11	35.178846766408384	37.0	37.0	37.0	33.0	37.0
12	35.17675494854055	37.0	37.0	37.0	33.0	37.0
13	35.23147539062929	37.0	37.0	37.0	33.0	37.0
14	35.29330606077988	37.0	37.0	37.0	33.0	37.0
15	35.33014475526097	37.0	37.0	37.0	33.0	37.0
16	35.35424337580838	37.0	37.0	37.0	33.0	37.0
17	35.360377259474504	37.0	37.0	37.0	33.0	37.0
18	35.357287129462044	37.0	37.0	37.0	33.0	37.0
19	35.351905030980625	37.0	37.0	37.0	33.0	37.0
20	35.332718643174196	37.0	37.0	37.0	33.0	37.0
21	35.26953817689652	37.0	37.0	37.0	33.0	37.0
22	35.29981949832984	37.0	37.0	37.0	33.0	37.0
23	35.303543031769024	37.0	37.0	37.0	33.0	37.0
24	35.30339291881818	37.0	37.0	37.0	33.0	37.0
25	35.29363557701343	37.0	37.0	37.0	33.0	37.0
26	35.24855775626295	37.0	37.0	37.0	33.0	37.0
27	35.24535168535349	37.0	37.0	37.0	33.0	37.0
28	35.210019002102804	37.0	37.0	37.0	33.0	37.0
29	35.22157037673469	37.0	37.0	37.0	33.0	37.0
30	35.18201256311151	37.0	37.0	37.0	33.0	37.0
31	35.156731345414784	37.0	37.0	37.0	33.0	37.0
32	35.140591152123	37.0	37.0	37.0	33.0	37.0
33	35.12147066756328	37.0	37.0	37.0	33.0	37.0
34	35.09463950313834	37.0	37.0	37.0	33.0	37.0
35	35.08046542337344	37.0	37.0	37.0	33.0	37.0
36	35.05686717932883	37.0	37.0	37.0	33.0	37.0
37	35.054261560222756	37.0	37.0	37.0	33.0	37.0
38	35.0271826484074	37.0	37.0	37.0	27.0	37.0
39	35.0174362904771	37.0	37.0	37.0	27.0	37.0
40	35.013522369880754	37.0	37.0	37.0	27.0	37.0
41	34.984714108054476	37.0	37.0	37.0	27.0	37.0
42	34.95690171751183	37.0	37.0	37.0	27.0	37.0
43	34.933301032606245	37.0	37.0	37.0	27.0	37.0
44	34.89305489618408	37.0	37.0	37.0	27.0	37.0
45	34.85894874558051	37.0	37.0	37.0	27.0	37.0
46	34.87224289495877	37.0	37.0	37.0	27.0	37.0
47	34.84411563334851	37.0	37.0	37.0	27.0	37.0
48	34.84586084895586	37.0	37.0	37.0	27.0	37.0
49	34.83242512964023	37.0	37.0	37.0	27.0	37.0
50	34.81447991964686	37.0	37.0	37.0	27.0	37.0
51	34.375853538577196	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	495.0
15	2179.0
16	2664.0
17	2432.0
18	2447.0
19	2438.0
20	2526.0
21	2664.0
22	3103.0
23	3604.0
24	4578.0
25	5531.0
26	7111.0
27	9192.0
28	11457.0
29	13474.0
30	16042.0
31	19609.0
32	24679.0
33	33652.0
34	50670.0
35	103506.0
36	495330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.026217287886126	17.366847981957157	21.86193757986192	14.744997150294795
2	28.81546236619505	23.729684408878388	24.978672977105944	22.476180247820615
3	31.841031605488517	24.65196373368742	24.557136284252906	18.949868376571153
4	29.407371156101604	25.838588303638225	24.78718743249494	19.966853107765235
5	24.639027170444102	29.74591857531826	25.379706437648814	20.23534781658882
6	25.584494674651538	35.4621709261725	23.16889659658548	15.784437802590487
7	77.47915199607509	6.9894054428759205	9.494339033150553	6.037103527898431
8	77.13303790779159	6.199176697588308	9.105631920603674	7.562153474016424
9	72.36628048177714	7.388486214627347	12.703949191037648	7.541284112557864
10	38.06644755871186	25.095712261543135	19.46684273410603	17.370997445638974
11	29.37625017848796	26.279895970504636	23.620089750458575	20.723764100548827
12	25.73216676450451	24.577273387414678	29.974261120867773	19.716298727213037
13	23.975967282699298	25.604875863912234	30.693216725267668	19.725940128120794
14	19.003079146138006	29.108365684911696	28.488386993628133	23.400168175322165
15	17.720528739307504	26.229370148026014	34.203540957037184	21.846560155629298
16	19.487345966416196	26.129050761365562	31.12268621633595	23.26091705588229
17	20.16212198690966	25.286831677005743	28.24344659335134	26.307599742733252
18	21.309570737982117	24.08556194111911	32.141135464123614	22.46373185677516
19	21.50728047811585	26.309552431524697	29.026596841769965	23.156570248589485
20	24.63109437222886	25.643075338394866	29.03013609020446	20.695694199171815
21	22.68829106779125	27.235615090867153	28.927131756455772	21.148962084885824
22	20.255484919750593	26.332618567873634	26.54546164614106	26.866434866234716
23	19.835046614342744	27.278452201229463	27.60943295137927	25.277068233048528
24	21.63005578587791	24.07140494738114	28.989373711683058	25.309165555057888
25	19.903634808142222	26.18152927263563	27.326048990520917	26.58878692870123
26	18.848084473317115	26.51983260575335	28.64142897765758	25.99065394327195
27	20.377161840067465	26.74610041946196	27.30383715551824	25.572900584952336
28	17.13435597272582	27.707311477050418	28.1044395600104	27.05389299021337
29	19.398254540306546	24.491965295838455	27.219261322238808	28.890518841616192
30	20.497984459038083	24.93437135015005	28.145812153779126	26.421832037032743
31	19.111453374063167	28.319113284019803	26.352877714084865	26.21655562783216
32	19.800264345245143	26.257562092452492	26.200567988352212	27.741605573950157
33	21.45565626819204	25.05214289288404	26.748419237401798	26.74378160152212
34	18.56323599586518	24.669782018909352	29.64486693036101	27.122115054864455
35	18.481345109673985	21.956520943197503	28.432369233923577	31.12976471320494
36	21.25733631281098	25.67444040210744	27.55585605266402	25.512367232417567
37	19.695429365754478	26.60867994576407	29.12313289389699	24.57275779458446
38	19.225685668362658	27.18814034462516	27.912465843201527	25.673708143810646
39	21.536570809987516	22.692806660621468	30.70041726518612	25.070205264204898
40	21.50361918663189	21.078543245344363	33.6556897079876	23.762147860036148
41	18.945108697642006	22.777138407801967	29.847946564671226	28.429806329884805
42	22.36951462258797	22.09003603931251	28.445061711067964	27.09538762703156
43	22.69207440232468	22.441886150920876	27.848881414430128	27.017158032324325
44	20.384606466084847	23.422380010324844	27.70902007974293	28.483993443847382
45	21.10844379246335	20.588296315642378	28.79959676976457	29.503663122129698
46	20.82689047734698	22.310567829696247	31.203844844230353	25.65869684872642
47	18.140112743369098	23.010118589231165	33.45675953735921	25.39300913004053
48	18.864438241945464	21.9976494508673	31.67273424027591	27.46517806691132
49	22.198654353336597	19.99992677417032	32.41341350748063	25.388005365012457
50	20.132099396741207	20.11550154201393	31.016386720251703	28.73601234099316
51	19.08435981708188	19.934999871854796	28.56661658833537	32.414023722727954
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	632.0
1	622.0
2	612.0
3	1269.5
4	1927.0
5	1494.5
6	1062.0
7	1097.0
8	1132.0
9	1141.5
10	1151.0
11	1258.5
12	1366.0
13	1468.5
14	1571.0
15	1625.5
16	1680.0
17	1698.5
18	1717.0
19	1934.0
20	2151.0
21	2211.0
22	2271.0
23	2686.0
24	3101.0
25	3468.0
26	4826.5
27	5818.0
28	6179.5
29	6541.0
30	8354.0
31	10167.0
32	11816.5
33	13466.0
34	14725.0
35	15984.0
36	17687.5
37	19391.0
38	22252.5
39	25114.0
40	27183.5
41	29253.0
42	34671.0
43	40089.0
44	45378.5
45	50668.0
46	60439.0
47	70210.0
48	87028.0
49	103846.0
50	106672.5
51	109499.0
52	92035.5
53	74572.0
54	65338.0
55	56104.0
56	46749.0
57	37394.0
58	34581.0
59	31768.0
60	28901.5
61	26035.0
62	25136.0
63	24237.0
64	19912.5
65	15588.0
66	12889.0
67	10190.0
68	8444.5
69	6699.0
70	5793.5
71	4888.0
72	3983.0
73	3078.0
74	2487.5
75	1575.0
76	1253.0
77	913.0
78	573.0
79	527.5
80	482.0
81	325.0
82	168.0
83	183.0
84	198.0
85	100.5
86	3.0
87	1.5
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	819383.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.253388817129437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.13193301172763	19.638365384375554
2	12.73888407606131	7.453110579467204
3	5.868561046602779	5.150258942799935
4	3.205192557629139	3.75050976488379
5	1.9151509538141414	2.801232774771069
6	1.261942282181719	2.2149652947262504
7	0.8991389109333275	1.8412002113500063
8	0.6399237838654155	1.497595141019496
9	0.46368674448123826	1.2207967763092749
>10	5.299553383417492	37.09180042892758
>50	0.4931447444881996	9.02161274943743
>100	0.07409729974330584	3.9744179052219977
>500	0.004604916933199798	0.892021323153911
>1k	0.002930401684763508	1.1902190060039624
>5k	0.0012558864363272175	2.261893717552545
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	7024	0.8572303794440451	TruSeq Adapter, Index 27 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	6065	0.7401910950068528	TruSeq Adapter, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT	5381	0.6567136491726091	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	2228	0.27191191420861793	TruSeq Adapter, Index 27 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	1598	0.19502479304549886	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCC	1321	0.16121886834361954	No Hit
GCCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTG	1321	0.16121886834361954	TruSeq Adapter, Index 27 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTG	1189	0.1451091858142041	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1035	0.12631455619655277	No Hit
CGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTG	1027	0.12533821180083063	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	831	0.10141777410563803	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2204304946526839E-4	0.0	0.0	0.677094838433309	0.0
2	1.2204304946526839E-4	0.0	0.0	2.8104073430862004	0.0
3	1.2204304946526839E-4	0.0	0.0	3.677645252586397	0.0
4	1.2204304946526839E-4	0.0	0.0	4.472389590704225	0.0
5	1.2204304946526839E-4	0.0	0.0	6.396398265524181	0.0
6	2.4408609893053677E-4	0.0	0.0	7.250577568731594	0.0
7	2.4408609893053677E-4	0.0	0.0	8.347622540374893	0.0
8	2.4408609893053677E-4	0.0	0.0	10.349860809902085	0.0
9	2.4408609893053677E-4	0.0	0.0	11.028908337126838	0.0
10	2.4408609893053677E-4	0.0	0.0	12.954991743787703	0.0
11	2.4408609893053677E-4	0.0	0.0	15.757222202559731	0.0
12	2.4408609893053677E-4	0.0	0.0	17.72394594469253	0.0
13	2.4408609893053677E-4	0.0	0.0	18.516249421821055	0.0
14	2.4408609893053677E-4	0.0	0.0	18.82709306880909	0.0
15	2.4408609893053677E-4	0.0	0.0	19.472944886579292	0.0
16	2.4408609893053677E-4	0.0	0.0	20.8514211302895	0.0
17	2.4408609893053677E-4	0.0	0.0	22.47923132405725	0.0
18	2.4408609893053677E-4	0.0	0.0	24.34404911988655	0.0
19	2.4408609893053677E-4	0.0	0.0	25.400209669958983	0.0
20	3.661291483958051E-4	0.0	0.0	26.355196532024706	0.0
21	0.0010983874451874155	0.0	0.0	27.542187231123908	0.0
22	0.0013424735441179521	0.0	0.0	28.757369874649584	0.0
23	0.0020747318409095623	0.0	0.0	29.962178858970713	0.0
24	0.0024408609893053677	0.0	0.0	30.949140999996338	0.0
25	0.0030510762366317097	0.0	0.0	31.771100938144922	0.0
26	0.0030510762366317097	0.0	0.0	32.496890953314875	0.0
27	0.0030510762366317097	0.0	0.0	33.18680031194204	0.0
28	0.0030510762366317097	0.0	0.0	33.92967635403712	0.0
29	0.0030510762366317097	0.0	0.0	34.679630893001196	0.0
30	0.0030510762366317097	0.0	0.0	35.51013384461235	0.0
31	0.0030510762366317097	0.0	0.0	36.2798593575898	0.0
32	0.003173119286096978	0.0	0.0	37.04860852617152	0.0
33	0.0032951623355622463	0.0	0.0	37.75682434221847	0.0
34	0.003539248434492783	0.0	0.0	38.46699284705687	0.0
35	0.003539248434492783	0.0	0.0	39.220364591405975	0.0
36	0.0036612914839580513	0.0	0.0	39.90636857245025	0.0
37	0.0036612914839580513	0.0	0.0	40.59261663959345	0.0
38	0.0036612914839580513	0.0	0.0	41.27105395157088	0.0
39	0.0036612914839580513	0.0	0.0	41.949735349647234	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATACGA	30	2.1647356E-6	45.000004	18
TCCGCTA	35	1.211356E-7	45.0	26
ATAGCCG	25	3.8898223E-5	45.0	1
TAACGCG	25	3.8898223E-5	45.0	1
TATTGCG	25	3.8898223E-5	45.0	1
ACGATTG	20	7.0321007E-4	45.0	1
GCGATTC	20	7.0321007E-4	45.0	9
CTCGACG	25	3.8898223E-5	45.0	1
TGCTACG	20	7.0321007E-4	45.0	16
ACGTACG	25	3.8898223E-5	45.0	1
CCGAAAT	20	7.0321007E-4	45.0	34
CCAACGG	20	7.0321007E-4	45.0	2
CGACTAT	20	7.0321007E-4	45.0	32
TATACGG	35	1.211356E-7	45.0	2
CGATCTA	25	3.8898223E-5	45.0	11
ACGGTTG	20	7.0321007E-4	45.0	1
ATAGGGA	580	0.0	41.508625	4
CGGGATC	120	0.0	41.250004	6
TTACGGG	180	0.0	41.25	3
TAAACGG	55	6.002665E-11	40.909092	2
>>END_MODULE