FastQCFastQC Report
Sat 18 Jun 2016
SRR3552772_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552772_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691234
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC27430.39682654499055314Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC21780.31508866751346143Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT19030.27530474484762035Illumina Single End Adapter 1 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA10370.15002155565264438No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9360.13541000587355367No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC9210.13323997372814417Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.11052697060619124No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG253.8891856E-545.0000042
TATCGCG253.8891856E-545.0000041
TCGAAAG253.8891856E-545.0000041
TTCGTAA207.031333E-445.024
CGGATAT207.031333E-445.014
TGCGACA207.031333E-445.030
GAACGAT406.8084773E-945.043
GGTTACG406.8084773E-945.01
TGCAACG207.031333E-445.01
TCGTTGA302.1642427E-644.99999624
TCGTAAG302.1642427E-644.9999961
GTTACGG603.6379788E-1241.2499962
CACAACG1150.041.0869612
GGCCGAT1250.039.68
GCTTACG403.4555524E-739.3751
CATACGA403.4555524E-739.37518
CGCATGG1200.039.3749962
CGTTAGG1150.039.1304362
GTTAGCG700.038.5714261
TACGGGA1400.038.5714264