##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552772_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	691234
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10455070207773	33.0	33.0	33.0	33.0	33.0
2	32.19366668884922	33.0	33.0	33.0	33.0	33.0
3	32.22727470002922	33.0	33.0	33.0	33.0	33.0
4	32.192154899787916	33.0	33.0	33.0	33.0	33.0
5	32.21876238726683	33.0	33.0	33.0	33.0	33.0
6	35.72289557515979	37.0	37.0	37.0	33.0	37.0
7	36.03739688730589	37.0	37.0	37.0	37.0	37.0
8	36.08248436853511	37.0	37.0	37.0	37.0	37.0
9	35.94884076882792	37.0	37.0	37.0	37.0	37.0
10	35.979659565357025	37.0	37.0	37.0	37.0	37.0
11	35.93824522520594	37.0	37.0	37.0	33.0	37.0
12	35.90665679060926	37.0	37.0	37.0	33.0	37.0
13	35.93210692761062	37.0	37.0	37.0	37.0	37.0
14	35.964735530949	37.0	37.0	37.0	37.0	37.0
15	35.994149593335976	37.0	37.0	37.0	37.0	37.0
16	35.99620823049792	37.0	37.0	37.0	37.0	37.0
17	35.994708014941395	37.0	37.0	37.0	37.0	37.0
18	35.98002847082175	37.0	37.0	37.0	37.0	37.0
19	35.97600957128845	37.0	37.0	37.0	37.0	37.0
20	35.947832427224355	37.0	37.0	37.0	37.0	37.0
21	35.85849365048594	37.0	37.0	37.0	37.0	37.0
22	35.90296628927396	37.0	37.0	37.0	37.0	37.0
23	35.94076246249461	37.0	37.0	37.0	37.0	37.0
24	35.94786570105059	37.0	37.0	37.0	37.0	37.0
25	35.9545219129846	37.0	37.0	37.0	37.0	37.0
26	35.90458657994254	37.0	37.0	37.0	37.0	37.0
27	35.89753252878186	37.0	37.0	37.0	37.0	37.0
28	35.88446170182601	37.0	37.0	37.0	37.0	37.0
29	35.87053298882867	37.0	37.0	37.0	37.0	37.0
30	35.85965534102778	37.0	37.0	37.0	37.0	37.0
31	35.85557857397061	37.0	37.0	37.0	37.0	37.0
32	35.84594652462118	37.0	37.0	37.0	37.0	37.0
33	35.819654415147404	37.0	37.0	37.0	37.0	37.0
34	35.798943628351616	37.0	37.0	37.0	37.0	37.0
35	35.79102879777326	37.0	37.0	37.0	37.0	37.0
36	35.78244704398221	37.0	37.0	37.0	33.0	37.0
37	35.774467112439496	37.0	37.0	37.0	33.0	37.0
38	35.74982856746051	37.0	37.0	37.0	33.0	37.0
39	35.739697410717646	37.0	37.0	37.0	33.0	37.0
40	35.73156123686046	37.0	37.0	37.0	33.0	37.0
41	35.73004655442296	37.0	37.0	37.0	33.0	37.0
42	35.711910872439724	37.0	37.0	37.0	33.0	37.0
43	35.72297803638131	37.0	37.0	37.0	33.0	37.0
44	35.69789680484467	37.0	37.0	37.0	33.0	37.0
45	35.66030461464569	37.0	37.0	37.0	33.0	37.0
46	35.67450241162906	37.0	37.0	37.0	33.0	37.0
47	35.67062818090545	37.0	37.0	37.0	33.0	37.0
48	35.67251610887196	37.0	37.0	37.0	33.0	37.0
49	35.65424443820761	37.0	37.0	37.0	33.0	37.0
50	35.66391699482375	37.0	37.0	37.0	33.0	37.0
51	35.34614761426666	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	566.0
15	2034.0
16	1424.0
17	753.0
18	554.0
19	503.0
20	510.0
21	545.0
22	673.0
23	887.0
24	1275.0
25	1871.0
26	2740.0
27	4070.0
28	5703.0
29	7301.0
30	9191.0
31	11556.0
32	15539.0
33	21169.0
34	32787.0
35	69841.0
36	499742.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.558476579566396	19.82512434284193	24.231736286120185	12.384662791471484
2	31.067771550589235	23.7229360824265	25.246153979694284	19.963138387289977
3	33.045104841486385	23.98724599773738	25.147634520292694	17.820014640483542
4	29.78267851407772	26.36429920981896	24.050466267573643	19.802556008529674
5	25.800958864870648	30.55086989355269	23.401915993715587	20.246255247861072
6	26.504338617602723	36.25877778002818	22.019605517089726	15.21727808527937
7	81.4306009253017	6.6452171044827075	7.368127146523464	4.556054823692122
8	82.1860614495236	5.591015488242766	7.127832253621784	5.095090808611845
9	75.93275215050186	7.746580752682883	9.787423651035684	6.5332434457795765
10	41.513148948113084	25.12723621812584	16.97485945425138	16.384755379509688
11	31.533315780184424	25.865915160423242	22.194654776819426	20.406114282572908
12	28.112766443780252	23.41782956278192	28.496862133517737	19.972541859920085
13	25.41469314298776	24.06637983663998	29.417389769600454	21.101537250771806
14	21.33749207938267	27.382044286016022	28.4906413747009	22.78982225990041
15	21.174160993238182	26.084075725441746	31.72138523278658	21.02037804853349
16	22.817309333742266	25.304310841191263	29.91635249423495	21.96202733083153
17	22.90324260670048	24.758909428645005	27.602085545560552	24.73576241909397
18	23.815379451820945	25.311399612866264	28.82048626080314	22.052734674509644
19	24.66834675377658	26.393666978186836	26.72220984500184	22.215776423034747
20	26.688936018772225	26.11995359024585	26.648573420867606	20.542536970114316
21	25.443771573736246	26.69385475830182	27.357161250748664	20.505212417213272
22	23.130227969110315	25.532164216459257	26.060928715890714	25.276679098539713
23	22.917130812431104	26.482493627338933	26.97610360601475	23.624271954215214
24	23.256523839973152	24.631745544924005	28.18669220553387	23.925038409568973
25	22.463594094040513	25.918719275961543	26.60227940176554	25.015407228232405
26	21.544368477245044	26.33362942216384	28.78258303266332	23.339419067927793
27	22.897166516693336	25.818897797272705	28.792709849341904	22.49122583669206
28	20.99665236374368	26.257244290645428	28.74887520000463	23.997228145606265
29	22.430609605430288	25.34337141980863	28.875460408486852	23.35055856627423
30	23.13254267006542	24.998914983927296	28.097431549952695	23.771110796054593
31	23.886845843809766	27.364828697662443	25.22778104086315	23.52054441766464
32	24.22826423468753	26.260716342078084	27.0012759789015	22.509743444332887
33	24.35296874864373	25.50221777285261	26.009137282020273	24.13567619648339
34	22.285072782878157	26.056299313980503	28.233130893445633	23.425497009695704
35	22.83351224042799	24.235353006362534	29.045880266306344	23.885254486903133
36	23.541232057450877	26.87049537493815	27.44106916037116	22.147203407239807
37	24.216112054673236	27.305369816878223	27.445409224661983	21.033108903786562
38	23.750133818648965	26.848505715864672	26.53558708049662	22.865773384989744
39	24.123379347659405	24.19846245989057	26.88192421090398	24.796233981546045
40	23.845615233046985	24.388412607018754	28.55111293715298	23.214859222781286
41	22.604067508253355	25.22995107300856	27.70031566734275	24.46566575139533
42	24.649973814945444	24.328809057424838	28.149078315013437	22.872138812616278
43	24.789289878680737	22.430609605430288	29.370372406449913	23.40972810943906
44	22.68305667834626	23.60358431442898	28.02726717725112	25.68609182997364
45	23.285168264292555	22.67784860119728	28.20347378745837	25.83350934705179
46	23.150336933657776	24.478975281887173	28.130994713801694	24.239693070653352
47	22.179319882991866	24.718546830740387	29.94586493141252	23.156268354855232
48	22.17107376083931	23.33088360815585	30.246486717956582	24.251555913048257
49	23.964388325805732	22.10105405694743	30.31361304565458	23.620944571592254
50	21.642164592598164	22.91119939123365	29.43214598818924	26.014490027978947
51	21.56650280512822	22.23964677663425	27.52367505070642	28.670175367531108
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	109.0
1	269.0
2	429.0
3	1002.0
4	1575.0
5	1230.5
6	886.0
7	866.0
8	846.0
9	892.5
10	939.0
11	983.5
12	1028.0
13	1079.5
14	1131.0
15	1179.0
16	1227.0
17	1282.0
18	1337.0
19	1414.5
20	1492.0
21	1737.5
22	1983.0
23	2200.0
24	2417.0
25	3591.5
26	5071.5
27	5377.0
28	6643.5
29	7910.0
30	9574.5
31	11239.0
32	11885.0
33	12531.0
34	13714.5
35	14898.0
36	16413.5
37	17929.0
38	19687.5
39	21446.0
40	24570.5
41	27695.0
42	30385.0
43	33075.0
44	37034.0
45	40993.0
46	47819.0
47	54645.0
48	62824.0
49	71003.0
50	71857.5
51	72712.0
52	63335.5
53	53959.0
54	48109.5
55	42260.0
56	38763.5
57	35267.0
58	33408.0
59	31549.0
60	31680.5
61	31812.0
62	29591.5
63	27371.0
64	23378.0
65	19385.0
66	16451.5
67	13518.0
68	11277.5
69	9037.0
70	7289.0
71	5541.0
72	5020.0
73	4499.0
74	3466.0
75	1972.5
76	1512.0
77	1180.0
78	848.0
79	645.5
80	443.0
81	282.5
82	122.0
83	77.5
84	33.0
85	27.5
86	22.0
87	13.5
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	691234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.80082736758681
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.04612123390378	20.958768331593532
2	12.054918060883884	7.426029002473776
3	4.530008279793461	4.185840089989719
4	2.2643989269495015	2.7898144176128166
5	1.3305649787486986	2.049125110589774
6	1.0229131799946956	1.8903943361427522
7	0.8144492754402023	1.7559998072742882
8	0.7195070761504688	1.7729130593814182
9	0.6237218358037361	1.7290033730986685
>10	8.44166553330155	48.75877434323042
>50	0.09500951846218124	1.9571749149050375
>100	0.05104989051109062	2.7905357130533206
>500	0.0037814733711918976	0.7911395139944563
>1k	0.0018907366855959488	1.1444879866599966
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	2743	0.39682654499055314	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	2178	0.31508866751346143	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT	1903	0.27530474484762035	Illumina Single End Adapter 1 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1037	0.15002155565264438	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	936	0.13541000587355367	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	921	0.13323997372814417	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	764	0.11052697060619124	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2809468284256851	0.0
2	0.0	0.0	0.0	1.2403903743160782	0.0
3	0.0	0.0	0.0	1.6725161088719593	0.0
4	0.0	0.0	0.0	2.1314055732212247	0.0
5	0.0	0.0	0.0	3.406516461863855	0.0
6	0.0	0.0	0.0	4.092391288622956	0.0
7	0.0	0.0	0.0	4.855374590948941	0.0
8	0.0	0.0	0.0	6.111244527902273	0.0
9	0.0	0.0	0.0	6.544527612935706	0.0
10	0.0	0.0	0.0	7.76871508056606	0.0
11	0.0	0.0	0.0	9.744023008127494	0.0
12	0.0	0.0	0.0	11.113023954261509	0.0
13	0.0	0.0	0.0	11.65741268513991	0.0
14	0.0	0.0	0.0	11.868484478483408	0.0
15	0.0	0.0	0.0	12.307409647094905	0.0
16	0.0	0.0	0.0	13.277558684902653	0.0
17	0.0	0.0	0.0	14.409736789567642	0.0
18	0.0	0.0	0.0	15.638842996727591	0.0
19	0.0	0.0	0.0	16.371879855446924	0.0
20	0.0	0.0	0.0	17.088713807480534	0.0
21	1.446688096939676E-4	0.0	0.0	17.961645405173936	0.0
22	1.446688096939676E-4	0.0	0.0	18.894325221270943	0.0
23	1.446688096939676E-4	0.0	0.0	19.8403145678598	0.0
24	1.446688096939676E-4	0.0	0.0	20.6100973042414	0.0
25	1.446688096939676E-4	0.0	0.0	21.340964130815326	0.0
26	1.446688096939676E-4	0.0	0.0	21.94567975533611	0.0
27	2.893376193879352E-4	0.0	0.0	22.540991907226786	0.0
28	2.893376193879352E-4	0.0	0.0	23.13037263792001	0.0
29	2.893376193879352E-4	0.0	0.0	23.731760879817834	0.0
30	2.893376193879352E-4	0.0	0.0	24.4448334427994	0.0
31	2.893376193879352E-4	0.0	0.0	25.08586093855337	0.0
32	2.893376193879352E-4	0.0	0.0	25.736436575747142	0.0
33	2.893376193879352E-4	0.0	0.0	26.360393151957226	0.0
34	2.893376193879352E-4	0.0	0.0	26.989413136506595	0.0
35	4.340064290819028E-4	0.0	0.0	27.64259281227486	0.0
36	4.340064290819028E-4	0.0	0.0	28.269876771107903	0.0
37	4.340064290819028E-4	0.0	0.0	28.894990697795535	0.0
38	5.786752387758705E-4	0.0	0.0	29.504914399465303	0.0
39	5.786752387758705E-4	0.0	0.0	30.14174649973815	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	25	3.8891856E-5	45.000004	2
TATCGCG	25	3.8891856E-5	45.000004	1
TCGAAAG	25	3.8891856E-5	45.000004	1
TTCGTAA	20	7.031333E-4	45.0	24
CGGATAT	20	7.031333E-4	45.0	14
TGCGACA	20	7.031333E-4	45.0	30
GAACGAT	40	6.8084773E-9	45.0	43
GGTTACG	40	6.8084773E-9	45.0	1
TGCAACG	20	7.031333E-4	45.0	1
TCGTTGA	30	2.1642427E-6	44.999996	24
TCGTAAG	30	2.1642427E-6	44.999996	1
GTTACGG	60	3.6379788E-12	41.249996	2
CACAACG	115	0.0	41.08696	12
GGCCGAT	125	0.0	39.6	8
GCTTACG	40	3.4555524E-7	39.375	1
CATACGA	40	3.4555524E-7	39.375	18
CGCATGG	120	0.0	39.374996	2
CGTTAGG	115	0.0	39.130436	2
GTTAGCG	70	0.0	38.571426	1
TACGGGA	140	0.0	38.571426	4
>>END_MODULE