FastQCFastQC Report
Sat 18 Jun 2016
SRR3552771_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552771_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1925020
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC39100.20311477283352902No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT31980.16612814412317795No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT26940.1399465979574238No Hit
CCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC23370.12140133609001465No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19540.10150543890453086No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT253.8917904E-545.016
CACGGTA207.034475E-445.04
CGACGCA253.8917904E-545.035
CGTAGAT207.034475E-445.015
TCGTAGC253.8917904E-545.018
ACGATAG850.042.352941
TAGTGCG1450.041.896551
GCGTTAG2400.038.4374961
GCTACGA4000.038.2510
TCGTAAG1300.038.0769271
GTATACG659.094947E-1238.0769271
TACGGGA6100.037.9918024
CACAACG4750.037.89473712
CGAATAT4100.037.86585214
CGACCAA5450.037.5688129
TCTAGCG601.5643309E-1037.4999961
TTACGCG601.5643309E-1037.4999961
CGTTAGG4700.036.8617022
CGTAAGG2750.036.8181842
ACACGAC5650.036.6371726
Produced by FastQC (version 0.11.5)