FastQCFastQC Report
Sat 18 Jun 2016
SRR3552769_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552769_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1681495
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT68550.40767293390702913No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC67880.4036883844436052No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC53080.3156714709231963No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC34700.20636397967285064No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC23310.13862663879464404No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT18870.11222156473852138No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG207.034219E-445.041
TCGTTAC253.8915794E-545.043
CGAGTAG207.034219E-445.019
AATGCCG207.034219E-445.038
TCGGACG207.034219E-445.01
CATCGCG253.8915794E-545.01
TAGGCGA502.1827873E-1145.05
AAATGCG700.045.01
TAACGCG1200.044.9999961
TATAGCG650.041.538461
GTAAGCG603.6379788E-1241.2499961
ACCGTCG451.9292202E-840.0000041
CTAACGG850.039.7058832
TAAACGG800.039.3752
TAAGACG403.4594996E-739.3751
CGTACCC356.250015E-638.57142636
GTACGAG356.250015E-638.5714261
TAGACCG659.094947E-1238.076921
TCGTAAG659.094947E-1238.076921
GCTTGCG900.037.5000041