##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552769_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1681495
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.755091748711713	31.0	31.0	34.0	30.0	34.0
2	31.92562749220188	33.0	31.0	34.0	30.0	34.0
3	31.966677867017147	33.0	31.0	34.0	30.0	34.0
4	35.58677962170569	37.0	35.0	37.0	33.0	37.0
5	35.46008522178181	37.0	35.0	37.0	33.0	37.0
6	35.43676609207878	37.0	35.0	37.0	33.0	37.0
7	35.82248177960684	37.0	35.0	37.0	35.0	37.0
8	35.801622365811376	37.0	35.0	37.0	35.0	37.0
9	37.40746181225636	39.0	37.0	39.0	35.0	39.0
10	36.84342445264482	39.0	37.0	39.0	32.0	39.0
11	36.65436828536511	39.0	35.0	39.0	32.0	39.0
12	36.527784501292004	39.0	35.0	39.0	32.0	39.0
13	36.47066568737939	39.0	35.0	39.0	32.0	39.0
14	37.52522249545791	39.0	36.0	41.0	32.0	41.0
15	37.63238546650451	39.0	36.0	41.0	33.0	41.0
16	37.70199970859265	39.0	36.0	41.0	33.0	41.0
17	37.69586469183673	39.0	36.0	41.0	33.0	41.0
18	37.657882420108294	39.0	36.0	41.0	33.0	41.0
19	37.63899922390492	39.0	36.0	41.0	33.0	41.0
20	37.58259346593359	39.0	36.0	41.0	32.0	41.0
21	37.53588205733588	39.0	36.0	41.0	32.0	41.0
22	37.47637013490971	39.0	36.0	41.0	32.0	41.0
23	37.43052581185195	39.0	36.0	41.0	32.0	41.0
24	37.424019696757945	39.0	36.0	41.0	32.0	41.0
25	37.411512969113794	39.0	36.0	41.0	32.0	41.0
26	37.3084689517364	39.0	35.0	41.0	32.0	41.0
27	37.267846767311234	39.0	35.0	41.0	32.0	41.0
28	37.19809693159956	39.0	35.0	41.0	32.0	41.0
29	37.18866544354875	39.0	35.0	41.0	32.0	41.0
30	37.11214722612913	39.0	35.0	41.0	31.0	41.0
31	37.0018489498928	39.0	35.0	41.0	31.0	41.0
32	36.88739484803701	39.0	35.0	40.0	31.0	41.0
33	36.81718887061811	39.0	35.0	40.0	31.0	41.0
34	36.7011278653817	39.0	35.0	40.0	30.0	41.0
35	36.66358151525874	39.0	35.0	40.0	30.0	41.0
36	36.61062506876321	39.0	35.0	40.0	30.0	41.0
37	36.553213658084026	39.0	35.0	40.0	30.0	41.0
38	36.48985813219784	39.0	35.0	40.0	30.0	41.0
39	36.43921926618872	39.0	35.0	40.0	30.0	41.0
40	36.31992185525381	38.0	35.0	40.0	30.0	41.0
41	36.255117023838906	38.0	35.0	40.0	30.0	41.0
42	36.18624854668018	38.0	35.0	40.0	30.0	41.0
43	36.09809068715637	38.0	35.0	40.0	30.0	41.0
44	35.99515728562975	38.0	35.0	40.0	30.0	41.0
45	35.95251606457349	38.0	35.0	40.0	29.0	41.0
46	35.80604164746253	38.0	34.0	40.0	29.0	41.0
47	35.7111623882319	38.0	34.0	40.0	29.0	41.0
48	35.645214526358984	38.0	34.0	40.0	29.0	41.0
49	35.58161100687186	38.0	34.0	40.0	28.0	41.0
50	35.48742577289852	38.0	34.0	40.0	28.0	41.0
51	33.965183958322804	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	5.0
11	4.0
12	13.0
13	22.0
14	34.0
15	66.0
16	124.0
17	273.0
18	514.0
19	1009.0
20	1569.0
21	2399.0
22	3428.0
23	5069.0
24	7154.0
25	9440.0
26	12344.0
27	16064.0
28	20359.0
29	26695.0
30	34386.0
31	44474.0
32	58312.0
33	78702.0
34	124277.0
35	141411.0
36	151803.0
37	212546.0
38	336926.0
39	391996.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.010000029735444	20.983470066815542	25.94173637150274	10.064793531946274
2	30.56637099723758	23.598167107246827	27.010309278350515	18.825152617165084
3	31.791887576234245	24.241047401270894	27.05663709972376	16.910427922771106
4	29.089708860270175	26.87685660676957	25.602811783561652	18.4306227493986
5	25.11461526796095	29.963752494060348	25.000431163934472	19.92120107404423
6	27.26127642365871	35.67723959928516	22.745414051186593	14.31606992586954
7	82.66286845931745	5.700462980859295	6.9709990217039	4.665669538119352
8	83.59334996535821	4.575154847323364	6.759877371029946	5.071617816288481
9	77.998923576936	7.253188382956834	9.03422252221981	5.713665517887357
10	41.45644203521272	28.584979437940643	15.502097835557048	14.456480691289597
11	29.69684715089846	25.928652776249706	24.31758643350114	20.056913639350697
12	27.81619927504988	22.50919568598182	30.12545383721034	19.549151201757958
13	24.777415335757762	24.362486953574052	30.469671334140152	20.39042637652803
14	19.826345008459732	28.37344149105409	29.00882845325142	22.79138504723475
15	18.705259307937283	26.505104088920874	33.663674289843264	21.125962313298583
16	21.697180187868533	25.303019039604635	31.756502398163537	21.243298374363288
17	21.501342555285625	25.66543462811367	27.568503028554947	25.26471978804576
18	22.358972224121988	25.606261095037453	29.601812672651423	22.43295400818914
19	23.309792773692458	26.735018540049182	27.787712719930774	22.167475966327583
20	25.22600424027428	26.003942919842167	27.837489852779818	20.93256298710374
21	24.29433331648325	27.294223295341347	27.062881542912702	21.348561845262697
22	21.879874754310897	25.855027817507636	26.65711167740612	25.607985750775352
23	21.63830400922988	27.235763412915293	27.59235085444798	23.533581723406847
24	22.207856698949445	25.342031941813687	28.86354107505523	23.586570284181636
25	21.357423007502252	26.906592050526466	27.24046161302888	24.4955233289424
26	20.955102453471465	27.733534741405713	28.349296310723492	22.96206649439933
27	22.16414559662681	26.756725413991717	28.937641800897417	22.141487188484057
28	19.39078022830874	27.594491806398473	29.56797373765607	23.446754227636717
29	21.79625868646651	25.204594720769315	29.17546588006506	23.823680712699115
30	22.597807308377366	25.12942351895189	28.044389070440296	24.228380102230457
31	21.948087862289213	28.712782375207773	25.424101766582712	23.915027995920298
32	22.99388341921921	28.445520206720804	26.062224389605674	22.49837198445431
33	23.44211549841064	26.535315299777878	25.394009497500736	24.62855970431075
34	21.081121264113182	26.28964106345841	27.61768545252885	25.011552219899553
35	21.867326397045485	25.467931810680376	27.786642243955527	24.87809954831861
36	21.589299997918516	27.676918456492583	27.854914822821357	22.878866722767537
37	21.797685987766837	28.35726540965034	28.029223994124276	21.815824608458545
38	22.69450697147479	28.523664952913926	25.27964698081172	23.502181094799568
39	24.31473183090048	24.205781164975214	25.917353307622086	25.562133696502222
40	22.414042265959754	24.64729303387759	28.224110092506965	24.714554607655685
41	19.59518166869363	26.857528568327588	27.17028596576261	26.37700379721617
42	22.7359581800719	26.274000220042282	27.430173744197873	23.559867855687944
43	23.35546641530305	24.986455505368735	27.805435044409887	23.85264303491833
44	21.904317289079064	25.373610983083505	27.145486605669362	25.576585122168073
45	22.590908685425767	23.79828664373073	26.482743035215684	27.12806163562782
46	22.13464803641997	24.91407943526445	27.760593995224486	25.190678533091088
47	20.45869895539386	25.573492636017352	29.641360812848088	24.326447595740692
48	21.17032759538387	24.88642547257054	28.560596374059987	25.382650557985603
49	22.81880112637861	23.288264312412466	29.55132188915221	24.341612672056712
50	20.849303744584432	23.05924192459686	29.04724664658533	27.044207684233374
51	20.384360346001625	23.00399347009655	26.706294101380024	29.9053520825218
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	334.0
1	722.5
2	1111.0
3	1640.0
4	2169.0
5	1708.0
6	1247.0
7	1341.5
8	1436.0
9	1494.0
10	1552.0
11	1627.0
12	1702.0
13	1878.5
14	2055.0
15	2244.0
16	2433.0
17	2535.5
18	2638.0
19	2762.0
20	2886.0
21	3501.5
22	4117.0
23	4411.0
24	4705.0
25	7354.5
26	11936.0
27	13868.0
28	17747.0
29	21626.0
30	23901.5
31	26177.0
32	30467.5
33	34758.0
34	41832.5
35	48907.0
36	51292.5
37	53678.0
38	56306.0
39	58934.0
40	67564.5
41	76195.0
42	84451.5
43	92708.0
44	103331.5
45	113955.0
46	128877.5
47	143800.0
48	164252.5
49	184705.0
50	178198.5
51	171692.0
52	151961.0
53	132230.0
54	113292.5
55	94355.0
56	84354.0
57	74353.0
58	72659.0
59	70965.0
60	68806.0
61	66647.0
62	60279.0
63	53911.0
64	45445.0
65	36979.0
66	30858.5
67	24738.0
68	20143.0
69	15548.0
70	13454.0
71	11360.0
72	9945.5
73	8531.0
74	6977.5
75	4094.0
76	2764.0
77	2483.0
78	2202.0
79	1564.0
80	926.0
81	795.0
82	664.0
83	487.0
84	310.0
85	203.5
86	97.0
87	75.5
88	54.0
89	39.0
90	24.0
91	18.5
92	13.0
93	8.0
94	3.0
95	4.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1681495.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.40679720025051
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.18390285321173	13.953785890995341
2	13.41422821511612	5.743113259977382
3	6.054726294107019	3.8883689364291976
4	3.2953924984017897	2.8217519563405586
5	1.9709070330751821	2.109540357879392
6	1.2929652018215265	1.6606946317424633
7	0.9101097635161622	1.3637774596290904
8	0.6484455143818783	1.1104913297427994
9	0.4781752444403115	0.9212580435522566
>10	4.373291139293845	22.49121764311703
>50	2.0164906182606215	30.588797670827727
>100	0.35080262931100986	10.287562644663616
>500	0.007227312411447906	0.9987953700864826
>1k	0.002501761988578121	0.9331610958413
>5k	8.339206628593738E-4	1.1276837091753906
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT	6855	0.40767293390702913	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	6788	0.4036883844436052	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	5308	0.3156714709231963	No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	3470	0.20636397967285064	No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	2331	0.13862663879464404	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1887	0.11222156473852138	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3788355005515926E-4	0.0	0.0	0.42634679258635916	0.0
2	2.3788355005515926E-4	0.0	0.0	1.5496923868343349	0.0
3	2.3788355005515926E-4	0.0	0.0	2.0836220149331397	0.0
4	2.3788355005515926E-4	0.0	0.0	2.69248496129932	0.0
5	2.3788355005515926E-4	0.0	0.0	4.176105192105834	0.0
6	2.3788355005515926E-4	0.0	0.0	4.969863127752387	0.0
7	2.3788355005515926E-4	0.0	0.0	5.819583168549416	0.0
8	2.3788355005515926E-4	0.0	0.0	7.274538431574284	0.0
9	2.3788355005515926E-4	0.0	0.0	7.733415799630686	0.0
10	2.3788355005515926E-4	0.0	0.0	8.960181267265142	0.0
11	2.3788355005515926E-4	0.0	0.0	10.98742488083521	0.0
12	2.3788355005515926E-4	0.0	0.0	12.460399822776756	0.0
13	2.3788355005515926E-4	0.0	0.0	13.023291773094776	0.0
14	2.3788355005515926E-4	0.0	0.0	13.234056598443647	0.0
15	2.3788355005515926E-4	0.0	0.0	13.652850588315754	0.0
16	2.9735443756894906E-4	0.0	0.0	14.557462258288012	0.0
17	2.9735443756894906E-4	0.0	0.0	15.69763811370239	0.0
18	2.9735443756894906E-4	0.0	0.0	16.95883722520733	0.0
19	2.9735443756894906E-4	0.0	0.0	17.68896131121413	0.0
20	3.568253250827389E-4	0.0	0.0	18.398984237241265	0.0
21	9.51534200220637E-4	0.0	0.0	19.26934067600558	0.0
22	0.001546243075358535	0.0	0.0	20.203449906184677	0.0
23	0.0017246557378999044	0.0	0.0	21.099973535455057	0.0
24	0.0017841266254136944	0.0	0.0	21.801848949892804	0.0
25	0.0023788355005515925	0.0	0.0	22.417729460985612	0.0
26	0.0024383063880653822	0.0	0.0	22.97729104160286	0.0
27	0.002557248163092962	0.0	0.0	23.521092837028952	0.0
28	0.002557248163092962	0.0	0.0	24.097306266150063	0.0
29	0.002616719050606752	0.0	0.0	24.674173875033823	0.0
30	0.002616719050606752	0.0	0.0	25.342388767138765	0.0
31	0.002616719050606752	0.0	0.0	25.962253827694997	0.0
32	0.002616719050606752	0.0	0.0	26.532163342739647	0.0
33	0.002616719050606752	0.0	0.0	27.09148703980684	0.0
34	0.0026761899381205416	0.0	0.0	27.651940683736793	0.0
35	0.0027356608256343313	0.0	0.0	28.259078974365075	0.0
36	0.002854602600661911	0.0	0.0	28.84480774548839	0.0
37	0.002854602600661911	0.0	0.0	29.431131225486844	0.0
38	0.002854602600661911	0.0	0.0	29.990930689654146	0.0
39	0.002854602600661911	0.0	0.0	30.57784887852774	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACTCG	20	7.034219E-4	45.0	41
TCGTTAC	25	3.8915794E-5	45.0	43
CGAGTAG	20	7.034219E-4	45.0	19
AATGCCG	20	7.034219E-4	45.0	38
TCGGACG	20	7.034219E-4	45.0	1
CATCGCG	25	3.8915794E-5	45.0	1
TAGGCGA	50	2.1827873E-11	45.0	5
AAATGCG	70	0.0	45.0	1
TAACGCG	120	0.0	44.999996	1
TATAGCG	65	0.0	41.53846	1
GTAAGCG	60	3.6379788E-12	41.249996	1
ACCGTCG	45	1.9292202E-8	40.000004	1
CTAACGG	85	0.0	39.705883	2
TAAACGG	80	0.0	39.375	2
TAAGACG	40	3.4594996E-7	39.375	1
CGTACCC	35	6.250015E-6	38.571426	36
GTACGAG	35	6.250015E-6	38.571426	1
TAGACCG	65	9.094947E-12	38.07692	1
TCGTAAG	65	9.094947E-12	38.07692	1
GCTTGCG	90	0.0	37.500004	1
>>END_MODULE