FastQCFastQC Report
Sat 18 Jun 2016
SRR3552768_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552768_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493395
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC20580.417110023409236No Hit
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT19760.40049047923063674No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC17000.34455152565388786No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC8490.17207308545891223No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC5960.12079571134689246No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCATA207.029369E-445.018
CAACGTA207.029369E-445.01
TAACGCG207.029369E-445.01
TCGGACG207.029369E-445.01
GCGATCA207.029369E-445.09
ATGCGTA207.029369E-445.012
AAATGCG207.029369E-445.01
GCATACC207.029369E-445.042
GCTACGA700.041.78571310
ACGGGTA603.6379788E-1241.2500045
CTCGTAA1000.040.50000433
CCGCTAT451.9244908E-840.00000425
ACGGGTC451.9244908E-840.0000045
AATGCGG900.040.0000042
GCGTATG451.9244908E-840.0000041
TATGGGC1700.039.7058834
CGTAAGG850.039.7058832
CGACAGG1250.039.62
GACCCAC403.4528784E-739.37512
CGATGCA403.4528784E-739.37510