##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552768_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	493395
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15096829112577	33.0	33.0	33.0	33.0	33.0
2	32.23004286626334	33.0	33.0	33.0	33.0	33.0
3	32.278494917865	33.0	33.0	33.0	33.0	33.0
4	32.26095927198289	33.0	33.0	33.0	33.0	33.0
5	32.29236210338573	33.0	33.0	33.0	33.0	33.0
6	35.76138996138996	37.0	37.0	37.0	33.0	37.0
7	36.06743481389151	37.0	37.0	37.0	37.0	37.0
8	36.09936865999858	37.0	37.0	37.0	37.0	37.0
9	35.984118201441035	37.0	37.0	37.0	37.0	37.0
10	35.97832568226269	37.0	37.0	37.0	37.0	37.0
11	35.93952918047406	37.0	37.0	37.0	33.0	37.0
12	35.91201572776376	37.0	37.0	37.0	33.0	37.0
13	35.92908521569939	37.0	37.0	37.0	33.0	37.0
14	35.972075112232595	37.0	37.0	37.0	37.0	37.0
15	36.011651921888145	37.0	37.0	37.0	37.0	37.0
16	36.02346395889703	37.0	37.0	37.0	37.0	37.0
17	36.02269378489851	37.0	37.0	37.0	37.0	37.0
18	36.00782942672706	37.0	37.0	37.0	37.0	37.0
19	36.00471427558042	37.0	37.0	37.0	37.0	37.0
20	35.97992683347014	37.0	37.0	37.0	37.0	37.0
21	35.89155747423464	37.0	37.0	37.0	37.0	37.0
22	35.92933653563575	37.0	37.0	37.0	37.0	37.0
23	35.96514354624591	37.0	37.0	37.0	37.0	37.0
24	35.97907964207177	37.0	37.0	37.0	37.0	37.0
25	35.97841080675726	37.0	37.0	37.0	37.0	37.0
26	35.92334539263673	37.0	37.0	37.0	37.0	37.0
27	35.91650503146566	37.0	37.0	37.0	37.0	37.0
28	35.921454412793	37.0	37.0	37.0	37.0	37.0
29	35.9218557139817	37.0	37.0	37.0	37.0	37.0
30	35.918582474487984	37.0	37.0	37.0	37.0	37.0
31	35.903813374679515	37.0	37.0	37.0	37.0	37.0
32	35.88875241946108	37.0	37.0	37.0	37.0	37.0
33	35.87627357391137	37.0	37.0	37.0	37.0	37.0
34	35.85875414221871	37.0	37.0	37.0	37.0	37.0
35	35.84263926468651	37.0	37.0	37.0	37.0	37.0
36	35.83336880187274	37.0	37.0	37.0	37.0	37.0
37	35.834055878150366	37.0	37.0	37.0	37.0	37.0
38	35.80227809361668	37.0	37.0	37.0	37.0	37.0
39	35.79704901752146	37.0	37.0	37.0	37.0	37.0
40	35.79830764397693	37.0	37.0	37.0	37.0	37.0
41	35.77857700219905	37.0	37.0	37.0	33.0	37.0
42	35.77119751922901	37.0	37.0	37.0	33.0	37.0
43	35.77066042420373	37.0	37.0	37.0	33.0	37.0
44	35.7560210379108	37.0	37.0	37.0	33.0	37.0
45	35.71196708519543	37.0	37.0	37.0	33.0	37.0
46	35.72639568702561	37.0	37.0	37.0	33.0	37.0
47	35.72651729344643	37.0	37.0	37.0	33.0	37.0
48	35.705777318375745	37.0	37.0	37.0	33.0	37.0
49	35.70110155149525	37.0	37.0	37.0	33.0	37.0
50	35.705775291602066	37.0	37.0	37.0	33.0	37.0
51	35.40684238794475	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	305.0
15	1282.0
16	895.0
17	453.0
18	311.0
19	254.0
20	236.0
21	290.0
22	368.0
23	539.0
24	808.0
25	1307.0
26	1920.0
27	2809.0
28	4018.0
29	4917.0
30	6402.0
31	8239.0
32	11213.0
33	15721.0
34	24414.0
35	51524.0
36	355170.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.879640044994375	20.216662106425886	24.509166083969234	12.394531764610504
2	29.777156233849144	23.43497603340123	26.86853332522624	19.919334407523383
3	31.965058421751337	24.146981627296586	26.2039542354503	17.684005715501776
4	29.835121961106214	26.529251411141175	25.09328225863659	18.542344369116023
5	25.56207501089391	29.63670081780318	24.640298341085742	20.16092583021717
6	27.023581511770487	35.81653644645771	22.378824268588048	14.781057773183756
7	82.13540874958197	6.090860264088611	7.02378418913852	4.7499467971908915
8	83.33992034779436	4.869729121697626	6.7408465833662685	5.049503947141742
9	77.93106942713241	7.3900221931717995	8.924289869171758	5.754618510524023
10	41.60925830217169	28.483263916334785	15.52468103649206	14.38279674500147
11	29.816272965879264	26.126126126126124	23.93376503612724	20.123835871867367
12	27.922658316359104	22.689528673780643	29.97213186189564	19.415681147964612
13	25.039167401372126	24.221364221364222	30.348301056962473	20.39116732030118
14	20.230646844820075	28.11641788019741	29.049949837351413	22.602985437631105
15	18.985397095633317	26.123085965605654	33.597624621246666	21.293892317514366
16	21.76349577924381	25.01423808510423	31.715967936440375	21.506298199211585
17	21.673507027837736	25.182054945834476	27.947182277890942	25.197255748436852
18	22.69277151166915	25.328590682921394	29.67764164614558	22.300996159263875
19	23.66015058928445	26.400348605073017	27.886581744849458	22.052919060793077
20	25.434793623770002	25.665035113854014	27.92346902583123	20.976702236544757
21	24.47207612561943	27.09026236585292	27.094923945317646	21.342737563210004
22	22.084942084942085	25.778534439951763	26.80286585798397	25.333657617122185
23	21.610069011643816	27.093505203741426	27.650259933724502	23.64616585089026
24	22.24890807567973	25.131182926458518	28.85659562824917	23.763313369612582
25	21.3184162790462	26.845630782638658	27.349892074301525	24.48606086401362
26	20.96433891709482	27.611751233798476	28.477791627397924	22.946118221708772
27	22.127707009596772	26.548505761104185	29.14743765137466	22.17634957792438
28	19.538706310359856	27.505142938213805	29.644402557788386	23.311748193637957
29	21.86564517273179	25.226238612065384	29.198512348118648	23.70960386708418
30	22.431115029540226	25.11496873701598	28.2205940473657	24.23332218607809
31	22.04724409448819	28.49846471893716	25.550927755652165	23.903363430922486
32	23.140891172387235	28.22181011157389	26.151866151866155	22.485432564172722
33	23.4757141843756	26.665653279826508	25.481206741049263	24.377425794748632
34	21.295108381722557	26.1994953333536	27.793552832922913	24.71184345200093
35	21.87071210693258	25.4143232095988	27.9271172184558	24.78784746501282
36	21.490489364505112	27.608711073278002	28.216135145269007	22.684664416947882
37	21.98076591777379	28.18370676638393	27.959950952076934	21.87557636376534
38	22.568935639801783	28.335106760303614	25.412904468022578	23.68305313187203
39	24.09104267371984	24.288855784918777	25.86690177241358	25.7531997689478
40	22.27647219773204	24.659552691048752	28.24207784837706	24.821897262842143
41	19.62302009546104	26.92913385826772	27.187952857244195	26.259893189027046
42	22.778504038346558	26.585595719453988	27.24591858450126	23.389981657698193
43	23.419369876062788	25.03937007874016	27.817266084982624	23.723993960214433
44	21.8970601647767	25.428510625361017	27.391846289484086	25.282582920378193
45	22.5332644230282	23.962747899755772	26.60647148836125	26.89751618885477
46	21.98380607829427	25.13766860223553	27.850707850707852	25.027817468762354
47	20.57013143627317	25.756847961572372	29.558467353742945	24.114553248411514
48	21.37678736103933	25.248127767812807	28.30612389667508	25.068960974472787
49	22.819850221425025	23.453216996524084	29.408080746663423	24.31885203538747
50	20.98095846127342	23.465174961237953	28.76762026368326	26.786246313805368
51	20.404746703959304	23.35390508618855	26.573029722636022	29.668318487216126
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	125.0
1	220.0
2	315.0
3	665.0
4	1015.0
5	773.0
6	531.0
7	556.5
8	582.0
9	576.0
10	570.0
11	601.0
12	632.0
13	694.5
14	757.0
15	796.0
16	835.0
17	852.5
18	870.0
19	872.5
20	875.0
21	1074.5
22	1274.0
23	1345.0
24	1416.0
25	2239.0
26	3473.0
27	3884.0
28	4851.5
29	5819.0
30	6668.5
31	7518.0
32	8619.5
33	9721.0
34	11591.5
35	13462.0
36	14504.0
37	15546.0
38	16170.0
39	16794.0
40	19473.5
41	22153.0
42	24457.0
43	26761.0
44	29762.0
45	32763.0
46	36579.0
47	40395.0
48	46479.5
49	52564.0
50	51830.5
51	51097.0
52	45310.5
53	39524.0
54	34242.0
55	28960.0
56	25602.0
57	22244.0
58	21918.0
59	21592.0
60	20820.0
61	20048.0
62	18286.0
63	16524.0
64	13921.0
65	11318.0
66	9449.5
67	7581.0
68	6160.5
69	4740.0
70	4170.0
71	3600.0
72	2996.5
73	2393.0
74	1978.0
75	1191.5
76	820.0
77	760.0
78	700.0
79	450.5
80	201.0
81	193.5
82	186.0
83	112.0
84	38.0
85	28.0
86	18.0
87	13.5
88	9.0
89	4.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	493395.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.398168582435726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.38412345453924	21.785344051701557
2	11.541605285048094	7.2477053690374245
3	4.177266818543227	3.9347558334730555
4	2.0858023966746417	2.6196150112175554
5	1.2833602521555352	2.014758077458836
6	1.0036509841366115	1.890768167870931
7	0.7752940531565651	1.70399693673788
8	0.7124070518107097	1.7894621369654948
9	0.615747436180006	1.7400007624844172
>10	8.292025884894178	49.741872459357765
>50	0.08796697522169813	1.87653659702411
>100	0.03751532766807714	2.197213143203879
>500	0.0012936319885543843	0.29346270375539385
>1k	0.0019404479828315764	1.164508749711715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	2058	0.417110023409236	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT	1976	0.40049047923063674	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	1700	0.34455152565388786	No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	849	0.17207308545891223	No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	596	0.12079571134689246	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.41163773447238017	0.0
2	0.0	0.0	0.0	1.4957589760739367	0.0
3	0.0	0.0	0.0	1.9842114330303307	0.0
4	0.0	0.0	0.0	2.498201238358719	0.0
5	0.0	0.0	0.0	3.8119559379401897	0.0
6	0.0	0.0	0.0	4.48849299243	0.0
7	0.0	0.0	0.0	5.230292159426018	0.0
8	0.0	0.0	0.0	6.569989562115547	0.0
9	0.0	0.0	0.0	7.035134121748295	0.0
10	0.0	0.0	0.0	8.287680256184194	0.0
11	0.0	0.0	0.0	10.309995034404484	0.0
12	0.0	0.0	0.0	11.832912777794668	0.0
13	0.0	0.0	0.0	12.418042339302183	0.0
14	0.0	0.0	0.0	12.624773254694514	0.0
15	0.0	0.0	0.0	13.051611791769272	0.0
16	0.0	0.0	0.0	13.976225944729881	0.0
17	0.0	0.0	0.0	15.106152271506602	0.0
18	0.0	0.0	0.0	16.34917256964501	0.0
19	0.0	0.0	0.0	17.09482260663363	0.0
20	0.0	0.0	0.0	17.811287102625684	0.0
21	6.080321040950963E-4	0.0	0.0	18.65989724257441	0.0
22	0.0012160642081901925	0.0	0.0	19.610454098643075	0.0
23	0.0014187415762218912	0.0	0.0	20.50466664639893	0.0
24	0.0014187415762218912	0.0	0.0	21.216672240294287	0.0
25	0.0018240963122852887	0.0	0.0	21.836459631735224	0.0
26	0.002432128416380385	0.0	0.0	22.407807132216583	0.0
27	0.0026348057844120835	0.0	0.0	22.95037444643744	0.0
28	0.0028374831524437824	0.0	0.0	23.5051024027402	0.0
29	0.0028374831524437824	0.0	0.0	24.080706127950222	0.0
30	0.0028374831524437824	0.0	0.0	24.766769018737524	0.0
31	0.0028374831524437824	0.0	0.0	25.397906342788232	0.0
32	0.0028374831524437824	0.0	0.0	25.973307390630225	0.0
33	0.0028374831524437824	0.0	0.0	26.53573708691819	0.0
34	0.003040160520475481	0.0	0.0	27.07506156325054	0.0
35	0.0036481926245705773	0.0	0.0	27.686539182602175	0.0
36	0.0036481926245705773	0.0	0.0	28.258292037819597	0.0
37	0.0036481926245705773	0.0	0.0	28.869972334539263	0.0
38	0.0036481926245705773	0.0	0.0	29.428753838202656	0.0
39	0.0036481926245705773	0.0	0.0	29.995237081851254	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCATA	20	7.029369E-4	45.0	18
CAACGTA	20	7.029369E-4	45.0	1
TAACGCG	20	7.029369E-4	45.0	1
TCGGACG	20	7.029369E-4	45.0	1
GCGATCA	20	7.029369E-4	45.0	9
ATGCGTA	20	7.029369E-4	45.0	12
AAATGCG	20	7.029369E-4	45.0	1
GCATACC	20	7.029369E-4	45.0	42
GCTACGA	70	0.0	41.785713	10
ACGGGTA	60	3.6379788E-12	41.250004	5
CTCGTAA	100	0.0	40.500004	33
CCGCTAT	45	1.9244908E-8	40.000004	25
ACGGGTC	45	1.9244908E-8	40.000004	5
AATGCGG	90	0.0	40.000004	2
GCGTATG	45	1.9244908E-8	40.000004	1
TATGGGC	170	0.0	39.705883	4
CGTAAGG	85	0.0	39.705883	2
CGACAGG	125	0.0	39.6	2
GACCCAC	40	3.4528784E-7	39.375	12
CGATGCA	40	3.4528784E-7	39.375	10
>>END_MODULE