FastQCFastQC Report
Sat 18 Jun 2016
SRR3552766_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552766_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080134
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC73890.6840817898519999No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC50340.46605328598118384No Hit
CTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGCT48230.4465186726832041Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC23150.21432525964371088No Hit
ACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC14340.13276130554171983No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCC13470.1247067493477661No Hit
GCCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTG11320.1048018116270759No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCG207.033099E-445.02
TTGACCG351.211829E-745.01
GTACGCG207.033099E-445.01
CTTCGCG302.1653796E-644.9999961
ACGATAG302.1653796E-644.9999961
TAGTACG302.1653796E-644.9999961
TGTAGCG700.041.7857131
CGCGAGG1350.041.6666642
GTTCGCG501.0822987E-940.51
CGTGCGG950.040.2631572
GGTCGTT403.4579716E-739.3758
TAGTGCG403.4579716E-739.3751
GCGTCCG403.4579716E-739.3751
TATCCGG403.4579716E-739.3752
TTCGCGG1150.039.1304322
TCATGCG356.2479394E-638.5714261
GACGTAG659.094947E-1238.076921
GGATCGC659.094947E-1238.076928
CGGTAGT1550.037.74193612
TTGCGAG900.037.51