FastQCFastQC Report
Sat 18 Jun 2016
SRR3552765_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552765_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095313
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC42040.3838172284999813No Hit
CCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC34350.3136089866549562No Hit
CTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGCT33420.30511826299879574No Hit
TCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC21660.19775169289509026No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC21260.194099768741903No Hit
ACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC14140.12909551881516973No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11360.10371464595051824No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG207.03314E-445.0000041
ACGCATT302.1654068E-645.00000417
CGTGCAA207.03314E-445.00000445
CGACAGT253.8906845E-545.00000416
TCTAGCG302.1654068E-645.0000041
GCCGATA502.1827873E-1145.0000049
TGCGACG406.8139343E-945.0000041
ATGACCG207.03314E-445.00000438
ACGCTAA253.8906845E-545.00000439
CCGCGTA207.03314E-445.00000433
CACGTAT302.1654068E-645.00000430
CGCCCAT207.03314E-445.00000424
CGGACTA253.8906845E-545.00000426
AGTAACG551.8189894E-1245.01
TAGCCCG700.041.7857171
AAGGGCG3550.040.563385
TATCCGT451.9281288E-840.027
CGTAAGG2100.039.6428572
CGACCAA800.039.37500429
CGTGTAC3350.038.95522318