##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552765_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1095313
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76620472869399	31.0	31.0	34.0	30.0	34.0
2	31.943202536626515	33.0	31.0	34.0	30.0	34.0
3	31.982082747123425	33.0	31.0	34.0	30.0	34.0
4	35.58753251353723	37.0	35.0	37.0	33.0	37.0
5	35.458551117351846	37.0	35.0	37.0	33.0	37.0
6	35.435330357623805	37.0	35.0	37.0	33.0	37.0
7	35.78488979862377	37.0	35.0	37.0	35.0	37.0
8	35.76133123591156	37.0	35.0	37.0	35.0	37.0
9	37.34299419435358	39.0	37.0	39.0	35.0	39.0
10	36.77380620881885	39.0	37.0	39.0	32.0	39.0
11	36.627199713689144	39.0	35.0	39.0	32.0	39.0
12	36.46407921753873	38.0	35.0	39.0	32.0	39.0
13	36.39459679561915	38.0	35.0	39.0	32.0	39.0
14	37.44982758353092	39.0	36.0	41.0	32.0	41.0
15	37.54375781169401	39.0	36.0	41.0	32.0	41.0
16	37.616050389249466	39.0	36.0	41.0	33.0	41.0
17	37.609137296827484	39.0	36.0	41.0	33.0	41.0
18	37.56458656110171	39.0	36.0	41.0	33.0	41.0
19	37.53640009750637	39.0	36.0	41.0	32.0	41.0
20	37.47454471918073	39.0	36.0	41.0	32.0	41.0
21	37.410793992219574	39.0	36.0	41.0	32.0	41.0
22	37.34310466505921	39.0	35.0	41.0	32.0	41.0
23	37.29983027682498	39.0	35.0	41.0	32.0	41.0
24	37.27590743467849	39.0	35.0	41.0	32.0	41.0
25	37.218655306747934	39.0	35.0	41.0	32.0	41.0
26	37.117391101904204	39.0	35.0	41.0	31.0	41.0
27	37.04776169003746	39.0	35.0	41.0	31.0	41.0
28	36.94301446253263	39.0	35.0	41.0	31.0	41.0
29	36.92553817949755	39.0	35.0	41.0	31.0	41.0
30	36.85039710110261	39.0	35.0	40.0	31.0	41.0
31	36.7247599544605	39.0	35.0	40.0	31.0	41.0
32	36.62691942851039	39.0	35.0	40.0	30.0	41.0
33	36.531170542119014	39.0	35.0	40.0	30.0	41.0
34	36.41973298956554	39.0	35.0	40.0	30.0	41.0
35	36.39117129076346	39.0	35.0	40.0	30.0	41.0
36	36.2974181809218	38.0	35.0	40.0	30.0	41.0
37	36.219240527593485	38.0	35.0	40.0	30.0	41.0
38	36.15913624689929	38.0	35.0	40.0	30.0	41.0
39	36.13888450150779	38.0	35.0	40.0	30.0	41.0
40	36.034857616042174	38.0	35.0	40.0	29.0	41.0
41	35.9128924791361	38.0	35.0	40.0	29.0	41.0
42	35.803542001236174	38.0	34.0	40.0	29.0	41.0
43	35.71353667855672	38.0	34.0	40.0	29.0	41.0
44	35.62877460598021	38.0	34.0	40.0	28.0	41.0
45	35.5747681256408	38.0	34.0	40.0	28.0	41.0
46	35.45502061967675	38.0	34.0	40.0	28.0	41.0
47	35.32356687083966	38.0	34.0	40.0	27.0	41.0
48	35.251820255945105	37.0	34.0	40.0	27.0	41.0
49	35.1860061918374	37.0	34.0	40.0	27.0	41.0
50	35.086361615355614	37.0	34.0	40.0	27.0	41.0
51	33.541084603213875	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	9.0
12	5.0
13	18.0
14	26.0
15	62.0
16	111.0
17	223.0
18	439.0
19	784.0
20	1258.0
21	2011.0
22	2938.0
23	4150.0
24	5680.0
25	7463.0
26	9577.0
27	12028.0
28	15176.0
29	19230.0
30	24128.0
31	30853.0
32	39419.0
33	52811.0
34	82308.0
35	95445.0
36	97918.0
37	138231.0
38	210839.0
39	242127.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.212226094276254	20.775705209378508	26.148598619755266	10.863470076589978
2	31.255084163157015	24.681255495004624	25.584011145672513	18.479649196165845
3	31.664738755040794	24.317797743658662	26.951200250521996	17.066263250778544
4	28.71599259754974	26.25130898656366	26.021602957328177	19.011095458558422
5	25.66070155288945	30.289424118950475	24.47574346328401	19.574130864876068
6	26.857710992200403	36.15980089709517	22.378808614523884	14.603679496180543
7	81.87495263910863	6.273366608448909	7.198672890762732	4.653007861679721
8	83.26524016422702	4.989441374292097	6.676630333064612	5.068688128416261
9	77.38244684396149	7.077429008876915	9.269313885619908	6.270810261541677
10	41.809053667764374	25.31879015404729	16.67961578105984	16.19254039712849
11	30.79265926725968	26.822652520329804	22.463076764358682	19.921611448051834
12	27.86618984710307	23.504970725263007	29.39552438435406	19.233315043279866
13	24.769449463304095	25.32152909716218	29.65079388266185	20.258227556871873
14	20.660578300449277	28.216409373393724	28.14811839172912	22.974893934427875
15	20.112515783159697	26.17416208882758	32.23845603950652	21.474866088506207
16	21.80107421348966	25.10926100575817	30.256739397779448	22.83292538297272
17	22.587972570397685	25.315229527997936	27.27658669257098	24.8202112090334
18	23.31415768825897	24.959623413581323	29.218953851547457	22.507265046612247
19	23.877010498368957	26.662241751901057	26.912398556394386	22.548349193335604
20	25.72351464832427	25.82047323459139	26.978133191151755	21.477878925932586
21	24.57388892490092	26.79836722471111	26.999588245551724	21.628155604836245
22	22.577473288457274	25.37977728740552	26.13937751126847	25.90337191286874
23	22.30558753525248	26.799371503853237	26.406424464970286	24.488616495923996
24	22.945678541202376	25.016684728474875	27.91585601558641	24.121780714736335
25	22.11997849016674	26.46202501020256	26.424501489528563	24.993495010102134
26	22.068486359606798	26.778555536180072	26.321152035993368	24.83180606821977
27	23.67359832303643	26.610019236510478	26.80466679387536	22.911715646577736
28	20.125114921488198	26.479097755618714	28.39526235879607	25.00052496409702
29	22.20835505467387	24.87745512013461	26.66434160828914	26.249848216902382
30	23.864502658144293	24.767532203123675	25.922635812776807	25.44532932595523
31	23.48990653813111	27.040763690378917	24.86932958889377	24.600000182596208
32	23.044006599026943	27.93740236809022	24.06088487948194	24.9577061534009
33	23.57271391830463	26.59057274039475	24.139218652567802	25.697494688732807
34	20.30396790689054	25.750447588954025	27.895770432743884	26.049814071411554
35	22.600023920103204	24.790721921496413	25.435560428845456	27.17369372955493
36	23.266500078059877	28.456158194050467	24.671943088413997	23.605398639475656
37	22.450477626030185	27.582891830919564	28.432420687054748	21.5342098559955
38	21.29692608414216	28.325328011262535	25.52238492558748	24.855360979007827
39	22.131755945560766	25.246573353918013	26.85058974010169	25.771080960419535
40	22.382277942469415	24.922099892907323	28.479074018111717	24.216548146511546
41	20.16656426062687	26.75682658746861	26.752626874692442	26.32398227721208
42	21.70612418550679	26.570761051863713	26.35648440217545	25.366630360454046
43	23.207430204882073	24.529791940751185	27.426132986644003	24.836644867722743
44	21.82061200770921	23.84806899945495	27.318675118436463	27.012643874399373
45	22.387299338180043	22.96165570937257	26.47078962817021	28.18025532427717
46	22.236292274445752	24.845318187586564	27.64890035998842	25.269489177979267
47	20.173685512725587	25.344536219327264	29.790114789105942	24.691663478841207
48	20.015100706373428	24.888502190698	28.69590701470721	26.400490088221357
49	21.387219908829714	24.321084475396532	29.578211890117256	24.713483725656502
50	20.53340004181453	23.614711046066283	29.167918211506667	26.68397070061252
51	20.030986576439794	23.858568281395364	27.117454097595843	28.992991044568996
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	448.0
1	703.0
2	958.0
3	1316.0
4	1674.0
5	1325.5
6	977.0
7	985.0
8	993.0
9	1064.0
10	1135.0
11	1282.5
12	1430.0
13	1555.0
14	1680.0
15	1790.0
16	1900.0
17	1887.0
18	1874.0
19	2151.5
20	2429.0
21	2641.0
22	2853.0
23	3184.0
24	3515.0
25	4842.0
26	7399.5
27	8630.0
28	10151.0
29	11672.0
30	14150.0
31	16628.0
32	18094.0
33	19560.0
34	22371.5
35	25183.0
36	28537.0
37	31891.0
38	33628.0
39	35365.0
40	39351.0
41	43337.0
42	49685.5
43	56034.0
44	62740.5
45	69447.0
46	79513.5
47	89580.0
48	102675.5
49	115771.0
50	113771.5
51	111772.0
52	97312.0
53	82852.0
54	73866.0
55	64880.0
56	60852.5
57	56825.0
58	54363.5
59	51902.0
60	49183.5
61	46465.0
62	43055.0
63	39645.0
64	33894.0
65	28143.0
66	24243.5
67	20344.0
68	17109.0
69	13874.0
70	12123.0
71	10372.0
72	8262.0
73	6152.0
74	5305.5
75	3720.0
76	2981.0
77	2388.0
78	1795.0
79	1381.0
80	967.0
81	734.0
82	501.0
83	300.0
84	99.0
85	88.5
86	78.0
87	50.5
88	23.0
89	13.5
90	4.0
91	6.5
92	9.0
93	17.0
94	25.0
95	18.0
96	11.0
97	6.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1095313.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.130176700978915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.7702427569631	15.906678667377715
2	12.547517161576675	5.804525782116677
3	5.165729884257169	3.58452735037187
4	2.6292435042564035	2.432594673734065
5	1.512841657114675	1.7496147424832094
6	0.9988737759085086	1.386247616324269
7	0.6713871718938094	1.087051274447202
8	0.48513513547072645	0.8977009125832998
9	0.3563036036078477	0.7417228779580559
>10	4.805805837063013	29.778869811650466
>50	1.9377179787732044	29.711112652799244
>100	0.11088514708355855	4.350163804519118
>500	0.004752220589295355	0.744705980727725
>1k	0.003564165441971517	1.8244838529071126
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	4204	0.3838172284999813	No Hit
CCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	3435	0.3136089866549562	No Hit
CTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGCT	3342	0.30511826299879574	No Hit
TCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	2166	0.19775169289509026	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2126	0.194099768741903	No Hit
ACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	1414	0.12909551881516973	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1136	0.10371464595051824	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8259620765936313E-4	0.0	0.0	0.32228230651877593	0.0
2	1.8259620765936313E-4	0.0	0.0	1.4074515686383708	0.0
3	1.8259620765936313E-4	0.0	0.0	1.94099768741903	0.0
4	1.8259620765936313E-4	0.0	0.0	2.526948917797926	0.0
5	1.8259620765936313E-4	0.0	0.0	3.967450400022642	0.0
6	1.8259620765936313E-4	0.0	0.0	4.739010675487281	0.0
7	1.8259620765936313E-4	0.0	0.0	5.574114431217378	0.0
8	1.8259620765936313E-4	0.0	0.0	6.85219658672909	0.0
9	1.8259620765936313E-4	0.0	0.0	7.291523062357518	0.0
10	1.8259620765936313E-4	0.0	0.0	8.437862054043	0.0
11	1.8259620765936313E-4	0.0	0.0	10.198272092086919	0.0
12	1.8259620765936313E-4	0.0	0.0	11.483110307282027	0.0
13	2.738943114890447E-4	0.0	0.0	11.970824777940187	0.0
14	2.738943114890447E-4	0.0	0.0	12.152599302665083	0.0
15	2.738943114890447E-4	0.0	0.0	12.512861620377006	0.0
16	2.738943114890447E-4	0.0	0.0	13.353443262336885	0.0
17	2.738943114890447E-4	0.0	0.0	14.360917838097421	0.0
18	2.738943114890447E-4	0.0	0.0	15.478224032765063	0.0
19	2.738943114890447E-4	0.0	0.0	16.102611764856256	0.0
20	2.738943114890447E-4	0.0	0.0	16.684180686251327	0.0
21	4.564905191484078E-4	0.0	0.0	17.436568359911732	0.0
22	0.0010955772459561787	0.0	0.0	18.201189979485317	0.0
23	0.0010955772459561787	0.0	0.0	18.935957119106593	0.0
24	0.001460769661274905	0.0	0.0	19.532955420048882	0.0
25	0.0020085582842529945	0.0	0.0	20.069057885736772	0.0
26	0.0020085582842529945	0.0	0.0	20.53075239680347	0.0
27	0.0021911544919123575	0.0	0.0	20.99335988890847	0.0
28	0.002556346907231084	0.0	0.0	21.458249833609205	0.0
29	0.002556346907231084	0.0	0.0	21.958746038803522	0.0
30	0.002556346907231084	0.0	0.0	22.530911255504133	0.0
31	0.002556346907231084	0.0	0.0	23.05998376719714	0.0
32	0.002556346907231084	0.0	0.0	23.572257427785484	0.0
33	0.002556346907231084	0.0	0.0	24.050842088060673	0.0
34	0.003286731737868536	0.0	0.0	24.547138580478823	0.0
35	0.003286731737868536	0.0	0.0	25.071828783188003	0.0
36	0.003286731737868536	0.0	0.0	25.578715855650394	0.0
37	0.003286731737868536	0.0	0.0	26.0502705619307	0.0
38	0.003286731737868536	0.0	0.0	26.526298875298657	0.0
39	0.003286731737868536	0.0	0.0	27.042772248663166	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	20	7.03314E-4	45.000004	1
ACGCATT	30	2.1654068E-6	45.000004	17
CGTGCAA	20	7.03314E-4	45.000004	45
CGACAGT	25	3.8906845E-5	45.000004	16
TCTAGCG	30	2.1654068E-6	45.000004	1
GCCGATA	50	2.1827873E-11	45.000004	9
TGCGACG	40	6.8139343E-9	45.000004	1
ATGACCG	20	7.03314E-4	45.000004	38
ACGCTAA	25	3.8906845E-5	45.000004	39
CCGCGTA	20	7.03314E-4	45.000004	33
CACGTAT	30	2.1654068E-6	45.000004	30
CGCCCAT	20	7.03314E-4	45.000004	24
CGGACTA	25	3.8906845E-5	45.000004	26
AGTAACG	55	1.8189894E-12	45.0	1
TAGCCCG	70	0.0	41.785717	1
AAGGGCG	355	0.0	40.56338	5
TATCCGT	45	1.9281288E-8	40.0	27
CGTAAGG	210	0.0	39.642857	2
CGACCAA	80	0.0	39.375004	29
CGTGTAC	335	0.0	38.955223	18
>>END_MODULE