##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552763_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	572559
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7862910197901	31.0	31.0	34.0	30.0	34.0
2	31.97018822514361	33.0	31.0	34.0	30.0	34.0
3	32.000653207791686	33.0	31.0	34.0	30.0	34.0
4	35.609175648273805	37.0	35.0	37.0	33.0	37.0
5	35.489308525409605	37.0	35.0	37.0	33.0	37.0
6	35.45394972395858	37.0	35.0	37.0	33.0	37.0
7	35.80027909787463	37.0	35.0	37.0	35.0	37.0
8	35.751623850118506	37.0	35.0	37.0	35.0	37.0
9	37.36589242331358	39.0	37.0	39.0	35.0	39.0
10	36.84203549328541	39.0	37.0	39.0	32.0	39.0
11	36.65831468896655	39.0	35.0	39.0	32.0	39.0
12	36.417050469907906	38.0	35.0	39.0	32.0	39.0
13	36.296224493894954	38.0	35.0	39.0	32.0	39.0
14	37.383768310340066	39.0	36.0	41.0	32.0	41.0
15	37.51378809869376	39.0	36.0	41.0	32.0	41.0
16	37.599981835933065	39.0	36.0	41.0	33.0	41.0
17	37.61157540096305	39.0	36.0	41.0	33.0	41.0
18	37.59809207435391	39.0	36.0	41.0	33.0	41.0
19	37.57867748127267	39.0	36.0	41.0	33.0	41.0
20	37.498708430048254	39.0	36.0	41.0	32.0	41.0
21	37.42607486739358	39.0	35.0	41.0	32.0	41.0
22	37.361400309837066	39.0	35.0	41.0	32.0	41.0
23	37.313560698548095	39.0	35.0	41.0	32.0	41.0
24	37.30240551628741	39.0	35.0	41.0	32.0	41.0
25	37.24497737351085	39.0	35.0	41.0	32.0	41.0
26	37.133560034861034	39.0	35.0	41.0	32.0	41.0
27	37.068406924002595	39.0	35.0	41.0	32.0	41.0
28	36.99722997979248	39.0	35.0	41.0	31.0	41.0
29	36.947568722175355	39.0	35.0	41.0	31.0	41.0
30	36.88344257971668	39.0	35.0	40.0	31.0	41.0
31	36.72970645819907	39.0	35.0	40.0	31.0	41.0
32	36.59587570887891	39.0	35.0	40.0	30.0	41.0
33	36.52193223755106	38.0	35.0	40.0	30.0	41.0
34	36.41380713603314	38.0	35.0	40.0	30.0	41.0
35	36.375290581407334	38.0	35.0	40.0	30.0	41.0
36	36.289725600331145	38.0	35.0	40.0	30.0	41.0
37	36.184489284073784	38.0	35.0	40.0	30.0	41.0
38	36.151587871293614	38.0	35.0	40.0	30.0	41.0
39	36.084096136817344	38.0	35.0	40.0	30.0	41.0
40	35.982841942926406	38.0	35.0	40.0	30.0	41.0
41	35.89982167776596	38.0	35.0	40.0	29.0	41.0
42	35.83774423247211	38.0	34.0	40.0	29.0	41.0
43	35.76461465106653	38.0	34.0	40.0	29.0	41.0
44	35.613662522115625	38.0	34.0	40.0	29.0	41.0
45	35.56995872914407	38.0	34.0	40.0	29.0	41.0
46	35.45504306106445	38.0	34.0	40.0	28.0	41.0
47	35.349113366482754	37.0	34.0	40.0	28.0	41.0
48	35.303670014793234	37.0	34.0	40.0	28.0	41.0
49	35.26779074296273	37.0	34.0	40.0	28.0	41.0
50	35.15507746799893	37.0	34.0	40.0	27.0	41.0
51	33.599695402569864	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	3.0
11	9.0
12	3.0
13	7.0
14	17.0
15	25.0
16	45.0
17	102.0
18	213.0
19	379.0
20	568.0
21	930.0
22	1362.0
23	2014.0
24	2823.0
25	3707.0
26	4796.0
27	6042.0
28	7680.0
29	9870.0
30	12325.0
31	15869.0
32	20719.0
33	28127.0
34	45419.0
35	52699.0
36	51527.0
37	71077.0
38	109057.0
39	125121.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.43038708674565	20.31703282980444	26.422255173702624	10.830324909747292
2	31.658396776576737	23.806629535122145	26.20655687885441	18.32841680944671
3	32.23004092154695	24.14283942790175	26.712181626696985	16.914938023854308
4	29.08818130533273	26.17319787131108	25.2435120223418	19.495108801014393
5	25.76817410956775	30.092095312448148	23.983030569775345	20.156700008208762
6	26.439022004719163	36.05794337352133	23.140671965683886	14.36236265607562
7	82.18541669941438	5.820884834576	7.25008252424641	4.743615941763206
8	82.82779591273564	5.0689972561779655	6.933084625339922	5.170122205746482
9	77.23902689504489	7.196812904870939	9.292142818469364	6.272017381614821
10	43.37387064040561	24.522712943120272	16.155714956886538	15.94770145958757
11	32.42565395007327	25.84537139403974	22.223211930997504	19.505762724889486
12	28.539242244030742	22.8717040514602	28.29385268592407	20.295201018584986
13	24.37128750050213	25.8006598446623	29.82958961434542	19.99846304049015
14	20.34113514939072	29.004696459229528	28.44929518180659	22.20487320957316
15	19.031925094182434	26.11695912560976	33.83825946321689	21.012856316990913
16	20.74965200092916	24.404471853555705	32.0057845567007	22.840091588814428
17	20.767117449904728	24.522188979651006	27.66509652280376	27.045597047640506
18	21.62152721378932	25.189718439497067	29.61249408357916	23.576260263134454
19	24.30561741235401	26.16114671151794	27.287144206972556	22.246091669155494
20	25.909644246269814	27.275267701669172	25.85777186281239	20.95731618924862
21	23.644899477608423	27.081401218040412	27.169951044346526	22.103748260004647
22	22.384243370552205	25.74948607916389	25.65307680081878	26.21319374946512
23	22.714689665169878	27.166632609041162	26.104558656837114	24.014119068951846
24	23.085131837941592	24.858224217940858	28.110465471680644	23.946178472436902
25	21.32461458120473	26.793745273412874	26.390118747587586	25.491521397794813
26	21.51935433728227	28.979720867194477	26.293884123732226	23.20704067179103
27	23.036053926320257	27.21483724821372	27.132400329049062	22.616708496416962
28	19.909039941735262	27.028131598664938	29.585247983177283	23.477580476422517
29	21.43953723546394	27.428265034695116	27.19335474597378	23.938842983867165
30	24.68706281798033	26.398152854116347	25.601029762871597	23.313754565031726
31	23.133860440583415	28.278483090825574	25.370835145373665	23.216821323217346
32	23.870727732862466	28.540639479948794	23.639310533936243	23.949322253252504
33	25.274775176008063	26.26139838863768	24.60742037065176	23.8564060647025
34	21.2617389648927	26.07381946664012	27.559430556501603	25.105011011965576
35	22.31350830220117	26.27938780108251	25.712459327335697	25.69464456938062
36	24.980657015259563	27.092753759874526	24.56445536617187	23.36213385869404
37	22.26879675282373	27.41411802102491	28.29577388531138	22.021311340839983
38	22.43768764441743	28.053004144551043	25.261152125807122	24.248156085224405
39	24.256888809712187	25.426025964136446	27.02690901723665	23.290176208914716
40	24.403947890086435	23.753359915746675	28.048987091286666	23.793705102880228
41	20.02972619415641	24.90590489364415	26.51901376102725	28.545355151172192
42	21.858184047408212	24.68461765512375	26.077312556435235	27.3798857410328
43	23.446317322756258	24.20536573523427	27.695835713000754	24.652481229008714
44	22.20417459161414	25.155835468484472	27.521705186714385	25.11828475318701
45	23.448063867653815	24.74993843429236	26.32095556964435	25.481042128409477
46	23.468498442955223	25.149198597873756	27.25448381738825	24.12781914178277
47	21.300162952638942	24.78312278734593	29.2127099565285	24.704004303486627
48	21.437965345056142	23.103121250386423	28.864798212935263	26.594115191622176
49	22.093443645109062	22.867512343706064	29.98066574798405	25.058378263200826
50	21.21877396041281	23.43583805337092	29.164679971845697	26.180708014370573
51	20.91085809497362	23.54272660110137	25.933746565856097	29.612668738068916
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	205.0
1	344.0
2	483.0
3	682.5
4	882.0
5	694.5
6	507.0
7	532.5
8	558.0
9	621.5
10	685.0
11	710.5
12	736.0
13	842.5
14	949.0
15	935.0
16	921.0
17	1085.5
18	1250.0
19	1145.5
20	1041.0
21	1361.0
22	1681.0
23	1791.0
24	1901.0
25	2553.0
26	3762.5
27	4320.0
28	5521.0
29	6722.0
30	7337.0
31	7952.0
32	8938.5
33	9925.0
34	11317.0
35	12709.0
36	13552.0
37	14395.0
38	15796.5
39	17198.0
40	19973.5
41	22749.0
42	26900.0
43	31051.0
44	34829.5
45	38608.0
46	44173.5
47	49739.0
48	55365.0
49	60991.0
50	59732.5
51	58474.0
52	51803.5
53	45133.0
54	39713.5
55	34294.0
56	31541.5
57	28789.0
58	26696.5
59	24604.0
60	23592.0
61	22580.0
62	21112.0
63	19644.0
64	17451.0
65	15258.0
66	13077.0
67	10896.0
68	8813.0
69	6730.0
70	5769.5
71	4809.0
72	4561.5
73	4314.0
74	3413.5
75	2038.0
76	1563.0
77	1178.5
78	794.0
79	631.0
80	468.0
81	344.5
82	221.0
83	142.5
84	64.0
85	47.0
86	30.0
87	20.5
88	11.0
89	6.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	572559.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.529040557498817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.629786977261	17.77581655752499
2	12.325199444397924	6.293010329905931
3	4.778704261495657	3.659872047120452
4	2.412705589874744	2.4637623542886575
5	1.384292953814177	1.7669835480690983
6	0.8875049610904363	1.3594290087995498
7	0.6572958859182988	1.1746093330919756
8	0.4751997996361619	0.9705115966261512
9	0.39259581446300235	0.9020335023117243
>10	5.751046861733699	39.26223241632655
>50	1.2301953433603041	19.44141116645543
>100	0.06998322644882936	3.1493285472446133
>500	0.0027444402528952684	0.42510680668312995
>1k	0.0027444402528952684	1.3558927855517549
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGCT	2359	0.41200994133355695	TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	2222	0.38808227623703406	TruSeq Adapter, Index 22 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	1939	0.3386550556361877	TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	1221	0.21325313199163756	TruSeq Adapter, Index 22 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	730	0.12749777752161787	TruSeq Adapter, Index 20 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	632	0.11038163752556504	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.746544897556409E-4	0.0	0.0	0.43314313459398945	0.0
2	1.746544897556409E-4	0.0	0.0	1.5661268096388319	0.0
3	1.746544897556409E-4	0.0	0.0	2.0960285315574465	0.0
4	1.746544897556409E-4	0.0	0.0	2.65562151673452	0.0
5	1.746544897556409E-4	0.0	0.0	4.086740405792241	0.0
6	1.746544897556409E-4	0.0	0.0	4.873034918672137	0.0
7	1.746544897556409E-4	0.0	0.0	5.688846040320736	0.0
8	1.746544897556409E-4	0.0	0.0	7.019189288789453	0.0
9	1.746544897556409E-4	0.0	0.0	7.479927832764833	0.0
10	1.746544897556409E-4	0.0	0.0	8.673167306775373	0.0
11	1.746544897556409E-4	0.0	0.0	10.6664989983565	0.0
12	1.746544897556409E-4	0.0	0.0	12.107573193330294	0.0
13	3.493089795112818E-4	0.0	0.0	12.669262032384436	0.0
14	3.493089795112818E-4	0.0	0.0	12.88705618110972	0.0
15	3.493089795112818E-4	0.0	0.0	13.313038481623728	0.0
16	3.493089795112818E-4	0.0	0.0	14.227180081004752	0.0
17	5.239634692669227E-4	0.0	0.0	15.324534240139444	0.0
18	5.239634692669227E-4	0.0	0.0	16.542225342715774	0.0
19	5.239634692669227E-4	0.0	0.0	17.218836836029126	0.0
20	8.732724487782045E-4	0.0	0.0	17.82680911486851	0.0
21	0.0013972359180451273	0.0	0.0	18.593367670406018	0.0
22	0.001571890407800768	0.0	0.0	19.36272069777962	0.0
23	0.00192119938731205	0.0	0.0	20.164210151268254	0.0
24	0.0038423987746241	0.0	0.0	20.79750733112221	0.0
25	0.0038423987746241	0.0	0.0	21.374216456295333	0.0
26	0.0038423987746241	0.0	0.0	21.839670671494115	0.0
27	0.0038423987746241	0.0	0.0	22.30267972383632	0.0
28	0.0038423987746241	0.0	0.0	22.776866663522885	0.0
29	0.0038423987746241	0.0	0.0	23.26677250728746	0.0
30	0.0038423987746241	0.0	0.0	23.825841528995266	0.0
31	0.0038423987746241	0.0	0.0	24.333736785204668	0.0
32	0.0038423987746241	0.0	0.0	24.843553240801384	0.0
33	0.0045410167336466635	0.0	0.0	25.3301406492606	0.0
34	0.004890325713157945	0.0	0.0	25.812012386496413	0.0
35	0.004890325713157945	0.0	0.0	26.317113170869728	0.0
36	0.004890325713157945	0.0	0.0	26.833566497077157	0.0
37	0.004890325713157945	0.0	0.0	27.330109211452445	0.0
38	0.005064980202913586	0.0	0.0	27.81477542052435	0.0
39	0.0052396346926692275	0.0	0.0	28.315684497143526	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAT	20	7.0303166E-4	45.000004	41
GATACGT	20	7.0303166E-4	45.000004	11
CGTATAG	20	7.0303166E-4	45.000004	15
CTATCGA	20	7.0303166E-4	45.000004	40
GTTTATA	20	7.0303166E-4	45.000004	28
ACTCTCG	20	7.0303166E-4	45.000004	44
ATTCGTT	20	7.0303166E-4	45.000004	36
TCAATTG	20	7.0303166E-4	45.000004	17
ACTACGC	20	7.0303166E-4	45.000004	36
GTCGTAA	20	7.0303166E-4	45.000004	26
TCGTAAG	20	7.0303166E-4	45.000004	1
GTATACG	20	7.0303166E-4	45.000004	1
CGATGAA	20	7.0303166E-4	45.000004	10
ACCGTAT	20	7.0303166E-4	45.000004	13
ACGTAGG	20	7.0303166E-4	45.000004	2
CAGTACG	20	7.0303166E-4	45.000004	1
GATTCGT	20	7.0303166E-4	45.000004	35
TTACCGT	20	7.0303166E-4	45.000004	38
TATCGCG	20	7.0303166E-4	45.000004	1
AGTACGA	20	7.0303166E-4	45.000004	33
>>END_MODULE