FastQCFastQC Report
Sat 18 Jun 2016
SRR3552762_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552762_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224419
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC12710.5663513338888418TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGCT9410.4193049608099136TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC7590.338206658081535TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC4020.1791292181143308TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.10426924636505822No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCC2270.10115008087550519No Hit
ACTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC2260.10070448580556904TruSeq Adapter, Index 20 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2250.10025889073563289No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCCC207.0211437E-445.015
TTGGACG207.0211437E-445.01
ACGCATT207.0211437E-445.017
ACTCTAG207.0211437E-445.041
CCATGTA253.8807426E-545.030
TGTGATA207.0211437E-445.037
CTACGAA207.0211437E-445.011
TACGAAT207.0211437E-445.012
CGCTACA207.0211437E-445.028
GGGCGAA207.0211437E-445.07
CGAATAT207.0211437E-445.014
GCGATAT207.0211437E-445.09
CATAAGG406.7720975E-945.02
CTCGAGG253.8807426E-545.02
CGTAAGG406.7720975E-945.02
CGCACGG253.8807426E-545.02
GCTACGA207.0211437E-445.010
TAGTGCG253.8807426E-545.01
AAAGGGT453.8198777E-1045.04
TATACGG207.0211437E-445.02