##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552762_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	224419
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.074690645622695	33.0	33.0	33.0	33.0	33.0
2	32.18436941613678	33.0	33.0	33.0	33.0	33.0
3	32.22664302042162	33.0	33.0	33.0	33.0	33.0
4	32.17747606040487	33.0	33.0	33.0	33.0	33.0
5	32.21839505567711	33.0	33.0	33.0	33.0	33.0
6	35.68825723312197	37.0	37.0	37.0	33.0	37.0
7	35.85708429321938	37.0	37.0	37.0	33.0	37.0
8	35.872626649258756	37.0	37.0	37.0	37.0	37.0
9	35.75792602230649	37.0	37.0	37.0	33.0	37.0
10	35.826293673886795	37.0	37.0	37.0	33.0	37.0
11	35.81222178157821	37.0	37.0	37.0	33.0	37.0
12	35.796340773285685	37.0	37.0	37.0	33.0	37.0
13	35.80942344453901	37.0	37.0	37.0	33.0	37.0
14	35.8799344084057	37.0	37.0	37.0	33.0	37.0
15	35.91581372343696	37.0	37.0	37.0	37.0	37.0
16	35.93264830517915	37.0	37.0	37.0	37.0	37.0
17	35.95570785004835	37.0	37.0	37.0	37.0	37.0
18	35.93698840116033	37.0	37.0	37.0	37.0	37.0
19	35.92755960948048	37.0	37.0	37.0	37.0	37.0
20	35.895619354867456	37.0	37.0	37.0	37.0	37.0
21	35.80199537472318	37.0	37.0	37.0	33.0	37.0
22	35.8513806763242	37.0	37.0	37.0	33.0	37.0
23	35.86998872644473	37.0	37.0	37.0	37.0	37.0
24	35.881311297171806	37.0	37.0	37.0	37.0	37.0
25	35.882327253931265	37.0	37.0	37.0	37.0	37.0
26	35.83781676239534	37.0	37.0	37.0	33.0	37.0
27	35.82153471853987	37.0	37.0	37.0	33.0	37.0
28	35.82288487160178	37.0	37.0	37.0	33.0	37.0
29	35.806170600528475	37.0	37.0	37.0	33.0	37.0
30	35.814400741470195	37.0	37.0	37.0	33.0	37.0
31	35.792009589205904	37.0	37.0	37.0	33.0	37.0
32	35.740895378733526	37.0	37.0	37.0	33.0	37.0
33	35.722077898930124	37.0	37.0	37.0	33.0	37.0
34	35.71496620161395	37.0	37.0	37.0	33.0	37.0
35	35.68889889002268	37.0	37.0	37.0	33.0	37.0
36	35.66778659560911	37.0	37.0	37.0	33.0	37.0
37	35.68961629808528	37.0	37.0	37.0	33.0	37.0
38	35.667630637334625	37.0	37.0	37.0	33.0	37.0
39	35.65496682544704	37.0	37.0	37.0	33.0	37.0
40	35.6357260303272	37.0	37.0	37.0	33.0	37.0
41	35.637633177226526	37.0	37.0	37.0	33.0	37.0
42	35.606717791274356	37.0	37.0	37.0	33.0	37.0
43	35.60901260588453	37.0	37.0	37.0	33.0	37.0
44	35.57561525539281	37.0	37.0	37.0	33.0	37.0
45	35.548656753661675	37.0	37.0	37.0	33.0	37.0
46	35.565121491495816	37.0	37.0	37.0	33.0	37.0
47	35.56849019022454	37.0	37.0	37.0	33.0	37.0
48	35.54346111514622	37.0	37.0	37.0	33.0	37.0
49	35.539727028460156	37.0	37.0	37.0	33.0	37.0
50	35.54577375355919	37.0	37.0	37.0	33.0	37.0
51	35.217543969093526	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	179.0
15	609.0
16	412.0
17	240.0
18	228.0
19	234.0
20	213.0
21	261.0
22	296.0
23	402.0
24	512.0
25	769.0
26	1050.0
27	1446.0
28	2130.0
29	2488.0
30	3144.0
31	3907.0
32	5203.0
33	7147.0
34	11236.0
35	23964.0
36	158349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.44604957690748	19.346846746487596	24.494806589459895	13.712297087145028
2	30.29066166411935	23.46503638283746	26.489290122494086	19.75501183054911
3	32.157259412081864	23.80725339654842	26.241984858679523	17.793502332690192
4	29.84283861883352	25.74291838035104	24.693096395581478	19.72114660523396
5	25.876596901331883	29.572808006452217	23.964548456235878	20.58604663598002
6	26.046368622977557	36.16583266122744	22.890664337689767	14.897134378105239
7	81.20301756981361	6.2806625107499805	7.573333808634741	4.9429861108016695
8	82.15168947370766	5.356052740632478	6.969998083941199	5.52225970171866
9	76.86247599356561	7.419157914436834	9.432356440408343	6.286009651589215
10	43.263270935170375	24.6489824836578	16.21876935553585	15.868977225635975
11	32.25840949295737	26.009384232172856	22.271732785548462	19.460473489321313
12	28.399110592240405	22.945472531291912	28.30553562755382	20.349881248913864
13	24.68641246953244	25.463975866571015	29.63118096061385	20.2184307032827
14	20.60119686835785	28.72974213413303	28.283701469126942	22.38535952838218
15	18.94759356382481	25.804410500002227	33.872800431336024	21.375195504836935
16	20.944305072208678	24.00286963225039	31.961197581309957	23.09162771423097
17	21.047683128433867	24.083076744838895	27.635360642369854	27.233879484357388
18	21.98165039502003	24.563428230230063	29.7871392350915	23.667782139658406
19	24.349988191730645	25.675187929720742	27.423702984150182	22.551120894398423
20	25.96838948573873	26.624751023754673	26.202772492525146	21.204086997981456
21	23.951180604137797	26.62653340403442	27.366221220128423	22.056064771699365
22	22.40273773610969	25.38555113426225	25.99601638007477	26.215694749553293
23	22.277079926387692	26.90592151288438	26.305704953680394	24.51129360704753
24	22.998052749544378	24.398112459283748	28.323359430351264	24.28047536082061
25	21.253993645814305	26.17559119325904	26.91305103400336	25.657364126923298
26	21.35380694148	28.32558740570094	26.77848132288264	23.542124329936414
27	22.76545212303771	26.797641910889897	27.86840686394646	22.568499102125934
28	19.779073964325658	26.471020724626705	30.330319625343666	23.41958568570397
29	21.321724096444598	27.022667421207654	27.710220614119123	23.945387868228625
30	24.28493131151997	26.099394436299956	26.045031837767745	23.570642414412326
31	22.97354502069789	27.879992335764797	26.041021482138323	23.10544116139899
32	23.724818308610235	27.945049215975477	24.299635948827863	24.030496526586433
33	25.113292546531262	25.8151047816807	24.863313712297085	24.208288959490954
34	21.249092100045004	25.72509457755359	27.91073839559039	25.11507492681101
35	22.274406355968075	26.0802338482927	26.13727001724453	25.508089778494693
36	25.045116500831032	26.460326442948233	25.249199042861793	23.245358013358942
37	22.36307977488537	27.116687980964176	28.503379838605465	22.016852405544984
38	22.367090130514796	27.990499913108962	25.786141102134845	23.856268854241396
39	24.168630998266636	25.213551437266897	27.391174544044844	23.226643020421623
40	24.409252336032154	23.360321541402467	28.26810564167918	23.9623204808862
41	20.11772621747713	24.537138121103826	26.67822243214701	28.66691322927203
42	21.879609124004652	24.562537040090188	26.315953640288924	27.241900195616235
43	23.202135291575136	24.022475815327578	27.86707007873665	24.908318814360637
44	22.12156724697998	24.861531332017343	27.85994055761767	25.15696086338501
45	23.259617055596895	24.615117258342654	26.867600336869874	25.257665349190578
46	23.210156002833983	25.239395951323196	27.63580623743979	23.91464180840303
47	21.308356244346513	24.924805831948273	29.242622059629532	24.52421586407568
48	21.428221318159334	23.379927724479657	28.724840588363733	26.46701036899728
49	21.94689397956501	23.043503446677864	29.9399783440796	25.069624229677522
50	21.276718994381046	23.620103467175237	29.044777848577887	26.05839968986583
51	21.12610786074263	23.695409033994448	25.875260116122075	29.30322298914085
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	103.0
1	129.0
2	155.0
3	332.0
4	509.0
5	413.5
6	318.0
7	302.5
8	287.0
9	309.0
10	331.0
11	352.0
12	373.0
13	413.5
14	454.0
15	447.0
16	440.0
17	465.5
18	491.0
19	470.5
20	450.0
21	537.0
22	624.0
23	701.0
24	778.0
25	999.0
26	1479.0
27	1738.0
28	2101.5
29	2465.0
30	2700.0
31	2935.0
32	3364.5
33	3794.0
34	4243.0
35	4692.0
36	5154.5
37	5617.0
38	6179.5
39	6742.0
40	7635.0
41	8528.0
42	10089.0
43	11650.0
44	13198.5
45	14747.0
46	16812.5
47	18878.0
48	21065.5
49	23253.0
50	23169.0
51	23085.0
52	20568.5
53	18052.0
54	15981.0
55	13910.0
56	12698.0
57	11486.0
58	10696.0
59	9906.0
60	9479.5
61	9053.0
62	8516.0
63	7979.0
64	7087.5
65	6196.0
66	5365.0
67	4534.0
68	3665.0
69	2796.0
70	2384.0
71	1972.0
72	1835.5
73	1699.0
74	1346.5
75	797.5
76	601.0
77	487.5
78	374.0
79	248.0
80	122.0
81	84.5
82	47.0
83	40.5
84	34.0
85	19.0
86	4.0
87	3.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	224419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.34343348825188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.82375816862447	23.948507033718176
2	9.85714094802817	6.573418471698029
3	3.497307193735049	3.4983668940686843
4	1.7640219700918094	2.352741969262852
5	1.2602066043913456	2.1009807547489294
6	0.9501663793449064	1.9009085683475997
7	0.8245466329898835	1.9245251070542158
8	0.7737641823357255	2.0639963639442294
9	0.7644095203731174	2.293923420031281
>10	8.395140921300566	49.043530182382064
>50	0.058800732336393643	1.35282663232614
>100	0.026727605607451656	1.6224116496375083
>500	0.0026727605607451653	0.7575116188914486
>1k	0.0013363802803725826	0.5663513338888418
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	1271	0.5663513338888418	TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGCT	941	0.4193049608099136	TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	759	0.338206658081535	TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	402	0.1791292181143308	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	234	0.10426924636505822	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCC	227	0.10115008087550519	No Hit
ACTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	226	0.10070448580556904	TruSeq Adapter, Index 20 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	225	0.10025889073563289	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4366831685374233	0.0
2	0.0	0.0	0.0	1.6865773397083135	0.0
3	0.0	0.0	0.0	2.2253017792611143	0.0
4	0.0	0.0	0.0	2.748875986436086	0.0
5	0.0	0.0	0.0	4.026397051943017	0.0
6	0.0	0.0	0.0	4.710385484295002	0.0
7	0.0	0.0	0.0	5.406850578605198	0.0
8	0.0	0.0	0.0	6.635801781489089	0.0
9	0.0	0.0	0.0	7.084961611984725	0.0
10	0.0	0.0	0.0	8.311684839518936	0.0
11	0.0	0.0	0.0	10.263391245839257	0.0
12	0.0	0.0	0.0	11.69998975131339	0.0
13	0.0	0.0	0.0	12.274361796461084	0.0
14	0.0	0.0	0.0	12.504288852548136	0.0
15	0.0	0.0	0.0	12.890174183112839	0.0
16	0.0	0.0	0.0	13.751955048369345	0.0
17	0.0	0.0	0.0	14.792865131740182	0.0
18	0.0	0.0	0.0	15.995080630427905	0.0
19	0.0	0.0	0.0	16.671048351521037	0.0
20	0.0	0.0	0.0	17.281959192403495	0.0
21	8.911901398722925E-4	0.0	0.0	18.010061536679157	0.0
22	8.911901398722925E-4	0.0	0.0	18.78316898301837	0.0
23	8.911901398722925E-4	0.0	0.0	19.543799767399374	0.0
24	0.0026735704196168774	0.0	0.0	20.180555122338127	0.0
25	0.0031191654895530237	0.0	0.0	20.7201707520308	0.0
26	0.0031191654895530237	0.0	0.0	21.196957476862476	0.0
27	0.0031191654895530237	0.0	0.0	21.667505870715047	0.0
28	0.0031191654895530237	0.0	0.0	22.122904032189787	0.0
29	0.0031191654895530237	0.0	0.0	22.647369429504632	0.0
30	0.0031191654895530237	0.0	0.0	23.20347207678494	0.0
31	0.0031191654895530237	0.0	0.0	23.695409033994448	0.0
32	0.0031191654895530237	0.0	0.0	24.199822653162165	0.0
33	0.00356476055948917	0.0	0.0	24.652992839287226	0.0
34	0.00356476055948917	0.0	0.0	25.13111634932871	0.0
35	0.00356476055948917	0.0	0.0	25.635975563566365	0.0
36	0.00356476055948917	0.0	0.0	26.12568454542619	0.0
37	0.00356476055948917	0.0	0.0	26.591776988579397	0.0
38	0.004010355629425316	0.0	0.0	27.03202491767631	0.0
39	0.004010355629425316	0.0	0.0	27.51549556855703	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCCCC	20	7.0211437E-4	45.0	15
TTGGACG	20	7.0211437E-4	45.0	1
ACGCATT	20	7.0211437E-4	45.0	17
ACTCTAG	20	7.0211437E-4	45.0	41
CCATGTA	25	3.8807426E-5	45.0	30
TGTGATA	20	7.0211437E-4	45.0	37
CTACGAA	20	7.0211437E-4	45.0	11
TACGAAT	20	7.0211437E-4	45.0	12
CGCTACA	20	7.0211437E-4	45.0	28
GGGCGAA	20	7.0211437E-4	45.0	7
CGAATAT	20	7.0211437E-4	45.0	14
GCGATAT	20	7.0211437E-4	45.0	9
CATAAGG	40	6.7720975E-9	45.0	2
CTCGAGG	25	3.8807426E-5	45.0	2
CGTAAGG	40	6.7720975E-9	45.0	2
CGCACGG	25	3.8807426E-5	45.0	2
GCTACGA	20	7.0211437E-4	45.0	10
TAGTGCG	25	3.8807426E-5	45.0	1
AAAGGGT	45	3.8198777E-10	45.0	4
TATACGG	20	7.0211437E-4	45.0	2
>>END_MODULE