SRR3552760_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552760_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	569500
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

$Duplication level graph$

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	5724	1.0050921861281825	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	3743	0.657243195785777	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT	3195	0.5610184372256365	TruSeq Adapter, Index 22 (95% over 23bp)
GCATGGGGGGCCAGATTCTGTGTGTTTCTCTAACCTCTTTGTAAATAAATG	1576	0.2767339771729588	No Hit
TCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1527	0.2681299385425812	TruSeq Adapter, Index 20 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	989	0.1736611062335382	TruSeq Adapter, Index 22 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	897	0.15750658472344162	TruSeq Adapter, Index 22 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	840	0.14749780509218613	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC	799	0.14029850746268657	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCC	785	0.13784021071115013	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG	752	0.13204565408252855	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	750	0.13169446883230904	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC	746	0.13099209833187006	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	714	0.1253731343283582	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	700	0.12291483757682177	No Hit
ACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	693	0.12168568920105356	TruSeq Adapter, Index 22 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	601	0.10553116769095698	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTTC	25	3.8883176E-5	45.000004	13
TAACGCA	25	3.8883176E-5	45.000004	24
ACCACGC	25	3.8883176E-5	45.000004	22
GCATAAG	20	7.030286E-4	45.0	1
ATTAGTC	35	1.2104829E-7	45.0	31
GACGTAC	20	7.030286E-4	45.0	8
CAACCTA	20	7.030286E-4	45.0	27
CGCGATT	20	7.030286E-4	45.0	14
GCGTTCC	20	7.030286E-4	45.0	12
CGAGGTT	20	7.030286E-4	45.0	27
GGACGTA	20	7.030286E-4	45.0	7
CGTACGC	20	7.030286E-4	45.0	10
TACTATC	20	7.030286E-4	45.0	12
CGCCGAT	20	7.030286E-4	45.0	26
AGTCGTT	20	7.030286E-4	45.0	35
ACACGAC	30	2.163566E-6	44.999996	26
GACACGA	30	2.163566E-6	44.999996	25
TAACCTC	260	0.0	44.134617	31
GACTCAC	75	0.0	42.000004	21
CACGGGA	135	0.0	41.666664	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content