##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552760_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	569500
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.187083406496928	33.0	33.0	33.0	27.0	33.0
2	31.377367866549605	33.0	33.0	33.0	27.0	33.0
3	31.4900825285338	33.0	33.0	33.0	27.0	33.0
4	31.415891132572433	33.0	33.0	33.0	27.0	33.0
5	31.52371202809482	33.0	33.0	33.0	27.0	33.0
6	34.85705531167691	37.0	37.0	37.0	27.0	37.0
7	35.156147497805094	37.0	37.0	37.0	33.0	37.0
8	35.24901316944688	37.0	37.0	37.0	33.0	37.0
9	35.139044776119405	37.0	37.0	37.0	33.0	37.0
10	35.232797190518	37.0	37.0	37.0	33.0	37.0
11	35.232997366110624	37.0	37.0	37.0	33.0	37.0
12	35.24076733977173	37.0	37.0	37.0	33.0	37.0
13	35.26220544337138	37.0	37.0	37.0	33.0	37.0
14	35.33341176470588	37.0	37.0	37.0	33.0	37.0
15	35.38044424934153	37.0	37.0	37.0	33.0	37.0
16	35.41188059701493	37.0	37.0	37.0	33.0	37.0
17	35.403244951712026	37.0	37.0	37.0	33.0	37.0
18	35.3927216856892	37.0	37.0	37.0	33.0	37.0
19	35.38885689201054	37.0	37.0	37.0	33.0	37.0
20	35.36011237928007	37.0	37.0	37.0	33.0	37.0
21	35.29273397717296	37.0	37.0	37.0	33.0	37.0
22	35.30479192273925	37.0	37.0	37.0	33.0	37.0
23	35.3083230904302	37.0	37.0	37.0	33.0	37.0
24	35.29986303775242	37.0	37.0	37.0	33.0	37.0
25	35.291606672519755	37.0	37.0	37.0	33.0	37.0
26	35.23616330114135	37.0	37.0	37.0	33.0	37.0
27	35.23101141352063	37.0	37.0	37.0	33.0	37.0
28	35.2122405618964	37.0	37.0	37.0	33.0	37.0
29	35.21035118525022	37.0	37.0	37.0	33.0	37.0
30	35.180920105355575	37.0	37.0	37.0	33.0	37.0
31	35.1195153643547	37.0	37.0	37.0	33.0	37.0
32	35.10299209833187	37.0	37.0	37.0	33.0	37.0
33	35.09309043020193	37.0	37.0	37.0	33.0	37.0
34	35.079195785776996	37.0	37.0	37.0	33.0	37.0
35	35.06344161545215	37.0	37.0	37.0	33.0	37.0
36	35.05912906057946	37.0	37.0	37.0	33.0	37.0
37	35.04781211589113	37.0	37.0	37.0	33.0	37.0
38	35.00438103599649	37.0	37.0	37.0	27.0	37.0
39	35.007278314310796	37.0	37.0	37.0	27.0	37.0
40	34.971453906935906	37.0	37.0	37.0	27.0	37.0
41	34.947657594381035	37.0	37.0	37.0	27.0	37.0
42	34.944712906057944	37.0	37.0	37.0	27.0	37.0
43	34.936446005267776	37.0	37.0	37.0	27.0	37.0
44	34.9055223880597	37.0	37.0	37.0	27.0	37.0
45	34.84821773485513	37.0	37.0	37.0	27.0	37.0
46	34.87041615452151	37.0	37.0	37.0	27.0	37.0
47	34.843250219490784	37.0	37.0	37.0	27.0	37.0
48	34.84555399473222	37.0	37.0	37.0	27.0	37.0
49	34.83307287093942	37.0	37.0	37.0	27.0	37.0
50	34.81878138718174	37.0	37.0	37.0	27.0	37.0
51	34.38059877085163	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	459.0
15	1841.0
16	1987.0
17	1694.0
18	1553.0
19	1637.0
20	1599.0
21	1787.0
22	2006.0
23	2384.0
24	3045.0
25	3781.0
26	4909.0
27	6275.0
28	7722.0
29	9029.0
30	10887.0
31	13641.0
32	17138.0
33	23051.0
34	33875.0
35	69196.0
36	350004.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.43985952589991	19.024056189640035	22.92326602282704	14.612818261633013
2	29.42616330114135	24.05513608428446	25.697805092186126	20.82089552238806
3	31.739420544337136	24.3441615452151	26.72835820895522	17.188059701492538
4	29.299209833187007	25.945917471466203	25.667427568042143	19.087445127304655
5	25.696224758560138	29.35627743634767	24.73959613696225	20.20790166812994
6	26.240386303775242	34.87603160667252	23.04354697102722	15.840035118525023
7	76.83353819139596	7.280597014925373	10.059174714661983	5.826690079016681
8	76.73415276558384	6.396663740122915	9.328533801580335	7.540649692712906
9	71.53397717295874	8.171553994732221	12.327831431079893	7.966637401229148
10	40.898331870061455	23.097629499561016	18.5369622475856	17.467076382791923
11	30.024758560140473	25.008077260755044	23.26286215978929	21.70430201931519
12	25.569446883230906	21.928358208955224	31.58612818261633	20.916066725197542
13	24.02669007901668	25.453028972783144	30.97067603160667	19.549604916593502
14	20.040386303775243	28.62739244951712	28.43582089552239	22.89640035118525
15	18.569095697980682	26.431606672519752	33.76733977172959	21.231957857769974
16	20.53713784021071	24.748551360842843	31.268129938542582	23.44618086040386
17	20.753819139596136	24.73028972783143	27.760842844600525	26.755048287971906
18	20.93977172958736	24.672168568920107	31.209306409130814	23.17875329236172
19	21.919402985074626	25.888147497805093	28.80649692712906	23.38595258999122
20	23.42791922739245	25.501668129938544	29.077963125548727	21.99244951712028
21	23.24108867427568	27.438454784899037	28.837928007023706	20.48252853380158
22	20.997014925373133	26.20070237050044	26.382440737489027	26.419841966637403
23	20.175943810359964	26.261633011413522	27.60860403863038	25.953819139596135
24	22.7982440737489	24.48340649692713	27.64073748902546	25.077611940298507
25	20.40017559262511	26.713432835820893	26.98244073748903	25.903950834064972
26	20.92396839332748	25.540825285338016	28.61316944688323	24.922036874451273
27	21.950658472344163	24.78595258999122	27.58121158911326	25.68217734855136
28	17.86022827041264	25.716769095697984	28.84021071115013	27.582791922739247
29	22.017910447761192	23.05531167690957	29.108165057067602	25.818612818261634
30	24.4342405618964	23.957506584723443	26.29604916593503	25.312203687445127
31	22.72484635645303	25.111150131694473	26.2955223880597	25.8684811237928
32	22.728884986830554	25.006496927129064	28.236698858647934	24.02791922739245
33	22.971027216856893	23.17928007023705	27.622124670763824	26.22756804214223
34	20.382967515364356	22.86707638279192	30.305882352941175	26.444073748902547
35	22.258999122036872	21.423880597014925	29.31150131694469	27.005618964003514
36	24.51764705882353	22.601755926251098	28.28709394205443	24.59350307287094
37	20.329236172080773	23.43178226514486	31.92361720807726	24.315364354697103
38	20.187884108867426	25.086742756804213	29.974187884108865	24.75118525021949
39	23.412993854258122	21.514661984196664	32.855838454784895	22.216505706760316
40	24.15171202809482	20.512028094820018	32.99385425812116	22.342405618964005
41	20.33239683933275	22.229675153643548	30.4558384547849	26.982089552238808
42	21.33784021071115	20.830553116769096	31.946093064091308	25.885513608428447
43	22.77787532923617	20.426865671641792	30.89411764705882	25.901141352063213
44	21.701316944688323	21.416856892010536	29.03652326602283	27.845302897278312
45	22.956277436347676	20.5387181738367	29.51273046532046	26.99227392449517
46	23.746268656716417	21.590517998244074	30.5513608428446	24.111852502194907
47	19.49464442493415	22.017208077260754	34.176294995610185	24.31185250219491
48	20.28042142230026	20.71922739244952	32.00193151887621	26.998419666374012
49	21.99262510974539	19.719402985074627	33.8414398595259	24.44653204565408
50	21.0647936786655	19.63441615452151	32.3819139596137	26.9188762071993
51	21.04670763827919	19.015627743634766	29.098331870061457	30.839332748024585
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	340.0
1	352.0
2	364.0
3	865.0
4	1366.0
5	1067.5
6	769.0
7	755.0
8	741.0
9	805.5
10	870.0
11	951.0
12	1032.0
13	1124.5
14	1217.0
15	1272.5
16	1328.0
17	1305.5
18	1283.0
19	1201.0
20	1119.0
21	1306.5
22	1494.0
23	1689.5
24	1885.0
25	2802.0
26	3930.0
27	4141.0
28	4462.0
29	4783.0
30	5777.0
31	6771.0
32	7749.5
33	8728.0
34	9659.0
35	10590.0
36	11086.0
37	11582.0
38	13506.5
39	15431.0
40	17381.0
41	19331.0
42	23534.5
43	27738.0
44	31624.0
45	35510.0
46	43416.0
47	51322.0
48	61482.5
49	71643.0
50	71461.0
51	71279.0
52	60402.0
53	49525.0
54	43639.0
55	37753.0
56	33983.0
57	30213.0
58	26839.5
59	23466.0
60	21381.5
61	19297.0
62	17509.5
63	15722.0
64	13780.0
65	11838.0
66	10023.5
67	8209.0
68	7231.5
69	6254.0
70	5382.5
71	4511.0
72	3612.5
73	2714.0
74	2011.5
75	1236.5
76	1164.0
77	878.5
78	593.0
79	429.5
80	266.0
81	210.0
82	154.0
83	105.5
84	57.0
85	49.0
86	41.0
87	39.5
88	38.0
89	19.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	569500.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.924602314801284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.64857309399135	20.542812493162586
2	12.532465593280847	7.50058097805719
3	5.33167655771878	4.786449019826494
4	2.8782923539132264	3.4452701614634624
5	1.7167022469218838	2.5685816016031584
6	1.0728249138451653	1.9262315340136515
7	0.7476350144243612	1.5660876338288807
8	0.5824092911074659	1.3942693136668998
9	0.45791929097035305	1.2332747407107232
>10	5.5933693108005205	40.30767001126196
>50	0.36109161406441814	6.600696242490694
>100	0.06704306840935438	3.7229778177377675
>500	0.007057165095721513	1.63068364259788
>1k	0.0023523883652405045	1.7670725723424687
>5k	5.880970913101261E-4	1.0073422372361607
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	5724	1.0050921861281825	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	3743	0.657243195785777	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT	3195	0.5610184372256365	TruSeq Adapter, Index 22 (95% over 23bp)
GCATGGGGGGCCAGATTCTGTGTGTTTCTCTAACCTCTTTGTAAATAAATG	1576	0.2767339771729588	No Hit
TCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1527	0.2681299385425812	TruSeq Adapter, Index 20 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	989	0.1736611062335382	TruSeq Adapter, Index 22 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	897	0.15750658472344162	TruSeq Adapter, Index 22 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	840	0.14749780509218613	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC	799	0.14029850746268657	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCC	785	0.13784021071115013	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG	752	0.13204565408252855	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	750	0.13169446883230904	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC	746	0.13099209833187006	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	714	0.1253731343283582	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	700	0.12291483757682177	No Hit
ACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	693	0.12168568920105356	TruSeq Adapter, Index 22 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	601	0.10553116769095698	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.511852502194908E-4	0.0	0.0	0.5740122914837577	0.0
2	3.511852502194908E-4	0.0	0.0	2.7320456540825284	0.0
3	3.511852502194908E-4	0.0	0.0	3.6010535557506587	0.0
4	3.511852502194908E-4	0.0	0.0	4.428797190517998	0.0
5	3.511852502194908E-4	0.0	0.0	6.492712906057945	0.0
6	3.511852502194908E-4	0.0	0.0	7.469710272168569	0.0
7	3.511852502194908E-4	0.0	0.0	8.664091308165057	0.0
8	3.511852502194908E-4	0.0	0.0	10.750307287093943	0.0
9	3.511852502194908E-4	0.0	0.0	11.450570676031607	0.0
10	3.511852502194908E-4	0.0	0.0	13.396488147497806	0.0
11	3.511852502194908E-4	0.0	0.0	16.044600526777874	0.0
12	3.511852502194908E-4	0.0	0.0	18.129762949956103	0.0
13	3.511852502194908E-4	0.0	0.0	18.87041264266901	0.0
14	3.511852502194908E-4	0.0	0.0	19.174714661984197	0.0
15	3.511852502194908E-4	0.0	0.0	19.733801580333626	0.0
16	3.511852502194908E-4	0.0	0.0	20.89534679543459	0.0
17	3.511852502194908E-4	0.0	0.0	22.373661106233538	0.0
18	3.511852502194908E-4	0.0	0.0	24.16505706760316	0.0
19	3.511852502194908E-4	0.0	0.0	25.150482879719053	0.0
20	3.511852502194908E-4	0.0	0.0	26.033011413520633	0.0
21	0.004038630377524144	0.0	0.0	27.091308165057068	0.0
22	0.004389815627743635	0.0	0.0	28.169622475856013	0.0
23	0.005443371378402107	0.0	0.0	29.26040386303775	0.0
24	0.006145741878841089	0.0	0.0	30.175592625109747	0.0
25	0.0064969271290605795	0.0	0.0	30.947322212467075	0.0
26	0.0064969271290605795	0.0	0.0	31.659877085162424	0.0
27	0.0064969271290605795	0.0	0.0	32.342756804214225	0.0
28	0.0064969271290605795	0.0	0.0	33.02036874451273	0.0
29	0.0064969271290605795	0.0	0.0	33.69833187006146	0.0
30	0.0064969271290605795	0.0	0.0	34.443195785777	0.0
31	0.006672519754170325	0.0	0.0	35.09043020193152	0.0
32	0.006672519754170325	0.0	0.0	35.79683933274802	0.0
33	0.006672519754170325	0.0	0.0	36.41545215100966	0.0
34	0.00684811237928007	0.0	0.0	37.064266900790166	0.0
35	0.00684811237928007	0.0	0.0	37.70289727831431	0.0
36	0.00684811237928007	0.0	0.0	38.35873573309921	0.0
37	0.007901668129938543	0.0	0.0	38.968568920105355	0.0
38	0.007901668129938543	0.0	0.0	39.60772607550483	0.0
39	0.007901668129938543	0.0	0.0	40.22177348551361	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTTC	25	3.8883176E-5	45.000004	13
TAACGCA	25	3.8883176E-5	45.000004	24
ACCACGC	25	3.8883176E-5	45.000004	22
GCATAAG	20	7.030286E-4	45.0	1
ATTAGTC	35	1.2104829E-7	45.0	31
GACGTAC	20	7.030286E-4	45.0	8
CAACCTA	20	7.030286E-4	45.0	27
CGCGATT	20	7.030286E-4	45.0	14
GCGTTCC	20	7.030286E-4	45.0	12
CGAGGTT	20	7.030286E-4	45.0	27
GGACGTA	20	7.030286E-4	45.0	7
CGTACGC	20	7.030286E-4	45.0	10
TACTATC	20	7.030286E-4	45.0	12
CGCCGAT	20	7.030286E-4	45.0	26
AGTCGTT	20	7.030286E-4	45.0	35
ACACGAC	30	2.163566E-6	44.999996	26
GACACGA	30	2.163566E-6	44.999996	25
TAACCTC	260	0.0	44.134617	31
GACTCAC	75	0.0	42.000004	21
CACGGGA	135	0.0	41.666664	4
>>END_MODULE